Objective:To study the expression of IQ motif containing GTPase activating protein 1 (IQGAP1 )in oral squamous cell cancer(OSCC)tissue,and to explore the effects of IQGAP1 on cell proliferation and invasion as well as its underlying mechanism. Methods:Expression levels of IQGAP1 in tumor and adjacent normal tissues were examined by western blot and RT-PCR.OSCC cell line SCC-4 cells was transfected with the recombinant plasmid-pcDNA3.1 -IQGAP1 by lipofectamine,and then treated with an Akt in-hibitor.The phosphorylation of Akt,cell proliferation and invasion were detected by western blot,MTT assay and Transwell invasion as-say respectively.Results:Protein and mRNA expression levels of IQGAP1 were higher in cancer tissue than in adjacent normal tissue (P <0.05).Transfection of pcDNA3.1 -IQGAP1 increased IQGAP1 expression,enhanced the capability of cell proliferation and inva-sion (P <0.05),increased p Akt level in the cells.Preconditioning with an Akt inhibitor reduced p Akt level.Furthermore,silencing Akt pathway blocked the increase of cell proliferation and invasion induced by IQGAP1 overexpression(P <0.05).Conclusion:IQ-GAP1 overexprission can mediate the ability of proliferation and invasion of OSCC cells by regulating the activation of Akt pathway.

The whole process of mini-implant was introduced, including preparation, operating methods, points for attention during the procedure, post-operation of implanting and the medical orders. Principles of implanting position and eight normal positions of implanting were introduced. These might be useful for clinical application.

0.05).Conclusions:Metronidazole and chlorhexidine were ideal root canal ultrasonic irrigations and they could kill bacteria of root canal and prevent the incidence of postoperative pain,so it can increase success of root canal therapy.

Objective:To explore the surgical options of unstable intertrochanteric fractures in the elderly.Methods: Eighty two elderly patients with unstable intertrochanteric fractures were randomly divided into two groups, 41 cases were in each group, and group PFNA were treated with proximal femoral nail anti rotation (PFNA) fixation, LCP group were treated with proximal femoral locking plate (LCP) fixation, compared the operation time, bleeding and the fluoroscopy times between the two groups, all the patients were followed up for 12 months and compared fracture healing time, hip function Harris score and the excellent and good rate, and compared of the incidence rate of the internal fixation complications.Results: All the patients were successfully completed operation, there was no significant difference in the operation time and the fluoroscopy times, the bleeding volume of the LCP group was more than that of the PFNA group (t=7.419,P<0.05), the difference was statistically significant. All the discharged patients were followed up for 12 months, the fracture healing time of the PFNA group was shorter than that of the LCP group, the difference was statistically significant(t=3.016,P<0.05); Harris score of the hip joint and the excellent rate of hip joint function of the group PFNA after 12 months were better than those of the LCP group, the difference was statistically significant;The complication rate of internal fixation of the LCP group was higher than that of the PFNA group, there was no statistically significant difference (x2=1.051,P>0.05).Conclusion: PFNA internal fixation for unstable intertrochanteric fractures in the elderly is the ideal internal fixation.

AIM: To study the effects of salvia miltiorrhiza compound and astragali in the treatment of patients with exacerbating chronic cor pulmonale. METHODS: 115 cases were randomly divided into two groups, with 56 cases in routine treatment group and 59 cases in salvia miltiorrhiza and astragali group. Besides routine treatment, such as oxygen therapy, ventilation improvement, administration of antibiotics, expectorant, and antiasthma, the patients in salvia miltiorrhiza and astragali group were treated with salvia miltiorrhiza compound and astragali, while subjects in routine treatment group were treated with routine therapy only. RESULTS: After treatment, the highly effective rates of two groups were 57.6 % and 33.9 %, and total effective rates were 98.3 % and 89.3 %, respectively. The therapeutic effect was significantly different (P

Objective The misdiagnosis reasons of multiple myeloma were explored in order to reduce the in- cidence of misdiagnosis.Methods Medical records of the patients with multiple myeloma in Huainan Xinkang Hos- pital from 1997 to 2006 were checked retrospectively,and all the records were statistically analyzed.Results Among atl the 21 cases with multiple myeloma,only 6 cases were diagnosed correct.And the other 15 cases were diagnosed incorrect with the misdiagnosis incidence of 71.4%.Conclusion The main misdiagnosis reasons of multiple myelo- ma were absence of characteristic clinical manifestations,and the manifestations were with variety and similarity to other common diseases.

We reseach an effective way to improve the mental health education of college students according to their mental puzzlement.

Objective To investigate the relationship between the root diameter and the diameter of the root canal and the diameter of the root canal of the maxillary central incisors of Han nationality.Methods November 2014 to 2015 during November,select the database in a local three grade hospital in 420 cases of Han nationality in CBCT image data as the research object,according to the age will be all of the subjects were divided into six (8～12 years old,13 to 17,18 22,23 to 27,28 to 32,33 to 37 years old),70 cases in each group,men and women,15 cases in each group.Of all the research object of the maxillary central incisor root diameter and root pipe diameter were measured,take the cemento enamel junction from the apical 3/4,analysis of maxillary central incisor root diameter and root tube diameter and age,men and women of the maxillary central incisor root diameter.Results The men and women of the maxillary central incisor root diameter compared with significant difference (P＜0.05);male and female maxillary central incisor root diameter compared with significant difference (P＜0.05);male and female maxillary incisor root diameter and root diameter ratio compared with the difference significant (P＜0.05);different genders maxillary central incisor root diameter and root diameter ratio and age has certain correlation (R2=0.623,P＜0.05).Conclusion The root diameter and the diameter of root canal of maxillary central incisors of Han nationality were correlated with the age,and the trend of male root canal diameter was higher than that of female.

Objective:To Study the expressions of Matrix metalloproteinase-9(MMP-9)in human dental pulps in patients under the effects of orthodontic forces.Methods:10 orthodontic patients were selected,the orthodontic teeth to be extracted were decided before orthodontic treatments had started.One side of the orthoodontic tooth underwent routine orthodontic forces.The teeth were extracted within 1 to 7 days after orthodontic forces had been used.There were 20 teeth as under-forces group.The other side of the pre-extracted teeth did not receive any orthodontic forces.There are also 20 teeth as homologized control group.20 third molares clinically diagnosed with endodontitis and without worth of remaining were as inflamed group.Iimmunohistochemical streptavidin peroxidase method(SP method) was used.The expression of MMP-9 was analyzed by analysing images and half-quantitative analysis.Results:There was expression of MMP-9 in control,under-forces and inflamed groups.In under-forces pulpal tissues,MMP-9 was mainly expressed in odontoblasts and fibroblasts.In under-forces pulps,the expression of MMP-9 was manifestly stronger than that of controls,but weaker than that of the inflamed one.The differences among the three groups had statistical significance(P

Acute retinal necrosis (ARN) syndrome is a kind of rare eye infectious disease caused by herpetic virus primarily.Visual prognosis of ARN patient is poor because of a high rate of complications including retinal detachment.The infection and antiinfection runs through the pathological process in ARN,such as invasion and dissemination of virus as well as the immunologic response of body.The multiple mechanisms are associated with the entry and spread of virus,including binding of viral surface protein and host receptor.Establishment of latency of the virus relies on access to related neuron,ganglion,ocular tissue which may occur after initial entry and replication.Activation of the immunologic system makes it possible to eliminate the virus and control infection.The relationship of viral invasion,dissemination and immune response with ARN pathogenesis and progression were reviewed.