There is a strong support for a role played by adiponectin in the function of ovary and placenta.There is evidence of direct effects of adiponectin on the late stages of folliculogenesis,and additive interactions of adiponectin with insulin and gonadotropins in inducing periovulatory changes in ovarian follicles.Adiponectin has demonstrated a potential effect on ovarian function as well as a possible effect on the formation of placenta,via multiple mechanisms,providing new strategies for the etiology investigation,prevention and treatment of reproductive and related metabolic diseases.

Polycystic ovary syndrome (PCOS)is the most common cause of anovulatory infertility in women of reproductive age, exerting significant impacts on glucose and lipid metabolism, cardiovascular and endometrial deseases, as well as breast, skin, bone, and psychology fields. As a complex, multi-system of endocrine and metabolic disease, PCOS would be recognized as a component of metabolic syndrome in an overall consideration. The clinical treatment of PCOS is expected to turn simply regulating menstruation and ovulation adjustment into the combination of insulin resistance improvement with antihyperandrogen and ovulation treatment comprehensively.

Schizophrenia and psychosomatic disorders (PSD) are obviously affected by psycho-social factors. However, there is a hypothesis that schizophaenia and PSD are mutually exclusive. The analysis of PSD was carried out in 500 schizophrenic patients, of which 6 (1.2%) had essential hypertension, 4 (0.8%) peptic ulcer, 2 (0.4%)asthma, 3 (0.6%) rheumatoid arthritis, 2 (0.4%) hyperthroidism, 5 (1.0%) neurodermatitis and 1 (0.2%)chronic urticaria. These 23 patients were 4.6%. The incidence of these 7 diseases is lower in schizophrenic patients than in any other population in the same area and lower than that reported in the data from north America or Roland. In this paper, we try to analyze the psychological and physiological factors.

The unbalance between matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) promotes tumor invasion and metastasis. Studies find that MMP and TIMP expression are close-ly associated with the invasion and metastasis of nasopharyngeal carcinoma, TIMP may become a new agent for nasopharyngeal carcinoma treatment.

AIM:.To report the curative effect and safty of intracameral bevacizumab(avastin)in patients with rubeosis iridis.METHODS:5 cases(5 eyes)with iris rubeosis secondary to diabetic retinopathy,retinal periphlebitis(Eales disease)and branch/central retinal vein occlusion,among which 2 cases were silicon oil eyes and were performed intracameral bevacizumab(avastin)0.03 cc(0.75mg),one case is combined with trabeculectomy.and another with cyclocryosurgery.RESULTS:The rubeosis of all cases disappeared quickly,meanwhile the intraocular pressure decreased to normal range with the help of anti-glaucoma operation and medicine.There are not any sign of rubeosis again in the 2-5 months follow-up visits and intraocular pressure were well-controlled.CONCLUSION:Intracameral bevacizumab(avastin)was an effective treatment for rubeosis,esp,for some cases that can not be injected to vitreous cavity.Short term study of intracameral bevacizumab demonstrated rapid regression of rubeosis and well-tolerated injection with no obvious side effects.

Objective To explore the causes of bleeding after tooth extraction,and provide clinical preventive interventions for the occurrence of bleeding after tooth extraction.Methods 120 cases of bleeding after tooth extraction patients were selected as research subjects,their clinical data were retrospectively analyzed.Results The location of bleeding after tooth extraction analysis were as following:bleeding mandibular molar proportion of 46.7％ ;maxillary molar tooth bleeding proportion of 36.6％ ; anterior mandibular molar tooth bleeding after the proportion 6.7 ％ ;maxillary premolar bleeding proportion was 5.8％ ;the anterior mandible proportion of 2.5 ％ ;the anterior maxilla proportion 1.7％ ;extraction postoperative bleeding time more concentrated within 8 hours after surgery,65.0％ ;occur 15.8％ in the proportion of 8 to 12 hours after surgery;14.2％ in the proportion of 13 to 24 hours after surgery;bleeding time of over 24 hours compared to 5.0％ ;retrospective analysis found the cause of the bleeding,gingival the tissue tearing lead to bleeding the proportion of 41.7％ ; extraction sockets tissue residues cause bleeding ratio of 15.0％ ;sutures become loose resulting in bleeding was 4.2％ ;alveolar bone fracture cause bleeding ratio of 13.3％ ;tooth fossa vascular ruptured bleeding ratio of 9.2％.Conclusion After tooth extraction bleeding occurred within 8h after surgery,to mandibular teeth bit bleeding is the most common,cause more bleeding after tooth extraction,strictly regulate the operation for bleeding has an important role in the prevention of tooth extraction.

Objective To investigate the characteristics of TCM syndrome types in restenosis after percutaneous coronary intervention (PCI) of coronary heart disease, and explore the distribution law. Methods Sixty-two patients with coronary heart disease after PCI were selected. After 6 months of PCI, 62 patients were divided into restenosis group and non-restenosis group by coronary angiography. TCM syndrome types of the two groups were observed. Results There were 21 cases (33.87%) in restenosis group and 41 cases in non-restenosis group. In the deficiency syndrome, the dominant syndrome types were qi deficiency syndrome in two groups. The incidence of yang deficiency in restenosis group was higher than that in non-restenosis group (P<0.05). In the sthenia syndrome group, the dominant syndrome types were blood stasis syndrome in non-restenosis group, and phlegm turbidity syndrome in restenosis group. There was statistical difference in blood stasis syndrome and phlegm turbidity syndrome between the two groups (P<0.05). Conclusion The dominant syndrome types were qi deficiency syndrome and phlegm turbidity syndrome in restenosis after PCI of coronary heart disease. Qi deficiency and phlegm turbidity might be one of the important mechanisms for restenosis after PCI of coronary heart disease.

Objective To investigate the changes of the platelet function in APP/PS-1/tau(3xTg) mice, a murine model for Alzheimer''s disease,and explore its mechanisms.Methods We assessed the change of function of platelet in 3xTg-AD mice by flow cytometry.Adhesion assay and Western blotting were used to compare with the data of wild type mice.Results Platelets from aged 3xTg-AD mice were normal in number and glycoprotein expression (P>0.05), but adhere more avidly on matrices such as fibrinogen, compared with the platelets from age-matching wild type mice (P<0.05).The washed platelets of 3xTg-AD mice were adherent to fibrinogen, and also showed increased phosphorylation of selected signaling proteins, including PI3 kinase effector Akt and p38MAP kinase (P<0.05).In contrast, activation induced by several agonists in 3xTg-AD mice was similar to that of wild type platelets (P>0.05).Conclusions These results demonstrate that Alzheimer''s mutations result in a significant hyper-activated adhesion state of circulating platelets, evident with the progression of the disease.

The aim of synthetic biology is to design artificial life systems. Such system is hoped to create a better production process with desired ability for bioproduction, biotransformation, adaption and environmental monitoring. However, to design a life system involves understanding the cellular regulation networks at multiple levels, in which the controls of protein level, subcelluar location, and activity are especially critical. Thus tuning protein expression has become essential tools in synthetic biology studies, such as part design, module assembly and compatibility optimization. Protein budget, just like budget for a factory, can be thought as the cost estimating criteria for an artificial cell factory. Protein budget control has provided a powerful optimization strategy for synthetic biology.
Artificial Cells ; metabolism ; Metabolic Engineering ; Metabolic Networks and Pathways ; genetics ; Protein Engineering ; Proteins ; economics ; metabolism ; Recombinant Proteins ; biosynthesis ; genetics ; Synthetic Biology ; economics

Objective To study the relationship between coagulation abnormal and inflammatory in the TNBS induced rats colitis model as well as the therapeutic effect of heparin on this model Methods Forty SD-rats were separated into 4 groups randomly, including normal control group, colitis group, heparin group and SASP group. PT, APTT and the activity of antithrombin (AT)were chosen as indexs of coagulation. The level of damage ancl inflammatory state of the colitis rats were assessed by macroscopical score, histological score and the level of TNFα in each group. Results Compared with normal control group, TNBS induced colitis group has a shorter PT [(14.83±0.45)s vs(16.68±1.08 )s, P < 0.05] and APTT[(12.49±1.30)s vs(29.06±1.60) s, P<0.05] and a lower activity level of AT [(111.33± 8.50)% vs(122.13±3.52)%,P<0.05]. In heparin group, PT, APTT were prolonged [PT: (17.83± 0.78)s vs (14.83±0.45)s,P<0.05, APTT:(53.34±9.49)s vs (12.49±1.30)s,P<0.05] and AT activity was higher than colitis group [(131.67±6.92)% vs (111.33±8.50) %, P < 0.05]. SASP group has a similar data in PT, APTT compared with colitis group and no statistical significance(P>0.05). The activity of AT in SASP group is higher than in colitis group [(122. 33±5.82)% vs (111.33±8.50)%,P <0.05]. The heparin therapy group showed lower macroscopical score(2.50±0.55 vs 4.75±1.16, P< 0.05), histological scores(3.83±0.41 vs 7.75±1.04, P<0.05) and the level of TNFα[(84.75± 18.03) ng/L vs (149.93±23.52)ng/L, P < 0.05] compared with the colitis group. Conclusion Coagulation was abnormality in the rat colitis model induced by TNBS; heparin therapy is effective in the colitis model It seemed that the abnormality of coagulation plays an important role in the pathogenesis of the rat colitis model.