As the great progress in computer technology, the image reconstruction of PET has been extensively studied, especially the fast methods being able to suppress the noise and meanwhile improve spatial resolution. This paper gives a brief description of the principle and research status of PET reconstructions, and that of 3D PET as well.

Objective: To observe the therapeutic effects of Erchen decoction, Taohongsiwu decoction on CYP2E1 activity and microsome protein content in non-alcoholic fatty liver rats.Methods: Experimental animal model of non-alcoholic fatty liver rats were established , then treated with Erchen decoction, TaohongSiwu decoction.Microsome protein content and cyp2e1 activity are tested after treatment.Results: Microsome protein content of non-alcoholic fatty liver rats reduced and cyp2e1 activity increased. Compared with model group, in Erchen decoction group, the microsome protein content in hepatocyte increased significantly (P

Objective To investigate the protection against podocyte injury by simvastatin in DM rats. Methods We divided DM rats induced by STZ into 3 groups of NC,DM and SVT,measured Scr,TC and UAER，observed renal pathomorphology and FPW，and detected nephrin,podocin,NF-κB and MCP-1 by immunohistochemistry stain,real time-PCR. Results The levels of Scr,UAER,NF-κB and MCP-1 were higher in DM than in NC and SVT group.The level of FPW was higher in DM than in SVT group.The levels of nephrin and podocin were lower in DM than in NC and SVT group (all P<0.01). Conclusions Simvastatin can suppress inflammatory cytokines,maintain podocyte protein,relieve podocyte injury.

Objective To test HBV DNA by using PCR-microfluidic chip assay. Methods Pooled sera ( 5?50ul ) negative for ELISA serological tests were tested for HBV DNA using PCR-microfluidic chips assay. Individual donor samples were tested if the pooled sera were positive. The sensitivity of PCR-microfluidic chips assay was determined by serial dilutions of the standard control serum. The specificity of PCR-microfludic chips assay was also determined by testing 56 various serum samples. Serial dilutions of the standard control sera were tested repeatedly for understanding the inter- and intra-assay variation of this method. Results Seven of 545 nonrenumerated donors (1.28%) were found positive for HBV DNA. The sensitivity of PCR-microfluidic chips assay was 4.81?102copies/ml. The HBV DNA was positive for all 37 samples from HBeAg positive patients. The HBV DNA tests of samples from HCV RNA positive patients, anti-HAV IgM positive patients were all negative. The inter- and intra assay CV ranges were 15.6%~40.2% and 11.9%~30.6% respectively. Conclusion It is necessary to test HBV DNA for improving blood safety and it is feasible to test pooled serum samples for HBV DNA by PCR-microfluidic chips assay, because it is convenient, time-saving, sensitive, specific and the results are reproducible.

OBJECTIVE:To observe the efficacy and safety of sequential chemotherapy on the basis of 5-FU combined with three-dimensional conformal intensity modulated radiotherapy in the treatment of postoperative advanced gastric cancer. METH-ODS:110 patients with advanced gastric cancer were randomly divided into control group(55 cases)and sequential group(55 cas-es). Control group was given 400 mg/(m2·d) 5-FU injection by intravenous injection,then given 600 mg/m2 for continuous infu-sion 22 h,d1-2+50 mg/d Pirarubicin hydrochloride for injection,intravenous injection,d1+150 mg/d Oxaliplatin for injection,intra-venous injection,d1,three-dimensional conformal intensity modulated radiotherapy was conducted for 2 weeks after the 4th chemo-therapy,and the total dosage was DT40-45 Gy/1.8 Gy/24-25F. sequential group was additionally given three-dimensional conformal intensity modulated radiotherapy;3-week was regarded as a treatment course,and it lasted 6 courses. The total survival rate and progression-free survival rate in 1,2 and 3 years,metastasis rate and incidence of adverse reactions in 2 groups were followed-up. RESULTS:The total survival rate and progression-free survival rate in 1,2 and 3 years in sequential group were significantly high-er than control group,lymphatic metastasis rate and distant metastasis rate in sequential group were significantly lower than control group,the differences were statistically significant (P<0.05). And there were no significant differences in the nausea,vomiting, hair loss and incidence of bone marrow suppression between 2 groups (P>0.05). CONCLUSIONS:Sequential chemotherapy on the basis of 5-FU combined with three-dimensional conformal intensity modulated radiotherapy can effectively prolong the survival time and reduce long-term metastasis rate of patients in the treatment of postoperative advanced gastric cancer,with similar safety.

Purpose:To study the efficacies and adverse effects of three different regimens in treatment of patients with gastrointestinal adenocarcinoma, which consisted of two doses of CF(20 mg/m 2, 200 mg/m 2) and two methods of administration of 5-Fu (continuous intravenous infusion and two hours per day intravenously) and DDP 20 mg/m 2. Methods:We selected 36 cases with GI adenocarcinoma and randomizedly divided them into A and B groups, who were hospitalized from January 1999 to November 2000. At the same time, 29 cases hospitalized from April 1997 to December 1998 with the same disease were designated as C group (control) . A group: CF 200 mg/(m 2.d) for 5 days, 2 hours intravenously ; 5-Fu 3 g/m 2 , continuous intravenous infusion during 5 days; DDP 20 mg/(m 2.d) for 5 days, 2 hours IV. B group: CF 20mg/(m 2.d) for 5 days, 2 hours, IV.; the dosage and usage of 5-Fu and DDP as A group. C group: CF 200 mg/m 2, usage as A, B groups; 5-Fu 600 mg/(m 2.d) for 5 days, 2 hours; dosage and usage of DDP as A, B groups. One cycle of these regimens consisted of three weeks and they were evaluated after three cycles. Results:There were 8 cases of gastral cancer and 12 of rectal cancer in A group whose response rates were 50% and 41.7% respectively. B group had 7 cases of gastric cancer and 9 cases of rectal cancer, their response rates were 42.9% and 44.4%. C group consisted of 14 cases of gastric cancer and 15 cases of rectal cancer, whose response rates were 35.7%and 40%, respectively. Adverse effects included hematological, renal and bladder and gastrointestinal toxicity. A group was similar to B group. C group had more renal and bladder toxicities(27.5%) and incidence of phlebitis was 34.5%. Conclusions:Combination regimen of low dose of CF, continuous intravenous infusion of 5-Fu and DDP is of better efficacy, tolerance and relative economy, especially suitable for to the elderly patients.

Purpose:To study the apoptosis and proliferation in ovarian cancer and the relationship between gene and the histological grading as well as response to chemotherapy. Methods:In our study apoptosis in fifty fresh samples of epithelial ovarian carcinomas were evaluated by TDT (terminal deoxynucleotidyl transferase) assay. We used the techniques of immunohistochemistry to detect the proliferation and the protein expression of bcl-2,bax and p53 gene.Results:In 66% (30/50) samples of fresh epithelial ovarian carcinoma apoptosis was observed in different degrees. Our results showed that PCAN and p53 expression in poorly differentiated ovarian carcinoma is higher than that in well middle differentiated (P

Betatrophin,discovered in 2013,is a protein secreted by liver,white adipose tissues,and brown adipose tissues.Betatrophin can improve glucose tolerance in mice by promoting islet β cell proliferation and increasing the number of β cells significantly and specifically.At the same time,Betatrophin is involved in lipid metabolism.Betatrophin may work as the new target for treatment of diabetes and disorders of lipid metabolism.

Objective To analyse the characteristics of maxillofacial injuries resulted in different causes. Methods A retrospective review was done of patients admitted to the Stomatology Hospital of Fourth Military Medical University from January, 1996 to July, 2003. Data was grouped by different causes. Results 1 188 patients were reviewed, the majority of victims caused by different causes were males, the ratio of male and female is 3.34∶1. Most industrial and sport injury occurred in men. Traffic accidents were the major cause of the midface fracture and mandibular fracture. While violence was the major cause of pure soft injure and alveolar bone fracture. Conclusions Traffic accidents and violence were the major causes of facial injury. The facial injury caused by traffic accidents, industrial accidents and fall was more serious than those caused by other causes. More emphasis should be put on the prevention and treatment of these injuries to minimize the maxillofacial malformation.

Objective To design a more reasonable method by comparing three existing injury severity scores for evaluating maxillofacial injury severity according to its features by retrospective analysis. Methods Nine hundred and eighty-four cases with maxillofacial trauma (≤30 days before hospitalization) selected from 1 159 cases were stratified by the number of fractures and the sites of trauma. The injury severity was evaluated with ISS, RFISS and MISS respectively. The results of ISS/RFISS/MISS were compared and analyzed with SPSS software. Results MISS could accurately differentiate single from multiple facial injuries. It was more meaningful for evaluating different sites of facial injury compared with ISS and RFISS. Conclusion MISS can be used to evaluate maxillofacial injury objectively and accurately.