The separation,purification and activity determination of basic fibroblast growth factor (bFGF) with nucleic acid molecular hybridization was described and the changes of bFGF in lung injury treated with prostacy-clin were observed.It was found that bioactive bFGF and bFGF-mRNA could only be found in the injured lung tissues but not in the normal lungs.Administration of prostacyclin could slightly elevate the activity of bFGF and signficantly increase the level of bFGF-mRNA.The findings of this study suggest that there is an increase of bFGF level after lung injury,prostacyclin can influence the expression of bFGF,and bFGF plays an important role in the repair of the injured lung tissues.

Objective To discuss the value of the mammography in the diagnosis of ductal carcinoma in situ (DCIS) and guidelines ofthe operation.Methods 107 pathologically proved DCIS were retrospectively reviewed.Results There were 62 microcacifications(57.9%),the range of microcacifications was varied , of them,13 were masses or local compact with microcacifications ; 17 were localized infiltration and bad structure (15.9%);20 were masses (18.7%) ;1 papillae enhancement (0.9%),7 were normal(6.5%).Conclusion Multiplicity microcacifications are the most frequently appearance of X-ray in DCIS;mammography could find the early microcarcinoma and the early carcinoma , especially the DCIS ; mammography could exactly reflect the multifocus , it is of value in confirming the range of the operation.

Objective:To establish the drug release determination conditions and method for methylphenidate hydrochloride bipolar controlled release osmotic pump tablets. Methods: The drug release of the tablets was determined by HPLC using a Diamonsil C18 (250 mm × 4. 6 mm, 5 μm) column with acetonitrile-KH2 PO4 (0. 02 mol·L-1 ,and pH was adjusted to 3. 0 by 1% H3 PO4 solution) (30∶ 70) as the mobile phase at a flow rate of 1 ml·min-1 , the column temperature was 35 ℃ and the injection volume was 20 μl. The effects of release medium, release apparatus and rotation speed on the release of methylphenidate hydrochloride bipolar controlled release osmotic pump tablets were studied as well. Results:The established drug release determination method had a good linear rela-tionship within the range of 1. 0-24. 0 μg·ml-1(r=0. 999 5), and the average recovery was 100. 5%(RSD=1. 58%, n = 6). Un-der the conditions of 900 ml pH 3. 0 phosphate buffer solution as the release medium and the rotation speed of 50 r·min-1 , the drug was quickly released in 0-2h, and then the release behavior was complied with a zero-level model in vitro in 2-10h with the release e-quation of Q=5. 505t+44. 221(r=0. 994 5). Conclusion:The method is simple, accurate and reliable, and suitable for the quality control of methylphenidate hydrochloride bipolar controlled release osmotic pump tablets.

Objective To study the expression law of zinc finger protein A20 mRNA of mouse liver tissues in the process of trauma with lipopolysaccharide (LPS) infection. Methods A total of 95 healthy mice (either sex, from Kunming, Yunnan province) with a mean body weight of 21 g (18-24 g) were randomized into 4 groups: control, trauma only (Group A), LPS only (Group B) and trauma plus LPS (Group C). The models with closed fracture of bilateral spines as well as endotoximia were made. The expression characteristics of zinc finger protein A20 mRNA of liver tissues were measured by reverse transcription-polymerase chain reaction (RT-PCR) and all result data expressed as total gray ratio of A20 mRNA to GAPDH mRNA (?s). Results In the control group, the A20 mRNA expressed at a low level. There was low expression of zinc finger protein A20 in the Group A at various time points, with no significant difference compared with the control group. After LPS infection, the expression of A20 mRNA in the Group B was elevated more obviously than that in the control group at 0.5 hour, reached peak during 0.5-2 hours and decreased after 2 hours. But expression of A20 mRNA at each time point was higher in the Group B than that in the Group A. In the Group C, after LPS infection, the expression of A20 mRNA was elevated more significantly than that in other two groups 0.5 hour, reached the highest level during 0.5-2 hours, much higher than that in the Group B (P

Objective To explore the effect of different additional filters on radiation dose and image quality in digital mammography.Methods Hologic company's Selenia digital mammography machine and the post-processing workstations and 5 M high resolution medical monitor were used in this study.Mammography phantoms with the thickness from 1.6 cm to 8.6 cm were used to simulate human breast tissue.The same exposure conditions,pressure,compression thickness,the anode were employed with the additional filters of Mo and Rh under the automatic and manual exposure mode.The image kV,mAs,pressure,filter,average glandular dose (AGD),entrance surface dose (ESD),signal-to-noise ratio (SNR),contrast-to-noise ratio (CNR) and image score according to ACR criteria were recorded for the two additional filters.Paired sample t test was performed to compare the indices of Mo and Rh groups by using SPSS 17.0.Results AGD and ESD of Rh and Mo group were both higher with the increase of the thickness of all the phantoms.AGD,ESD and their increased value of Rh filter(1.484 ± 1.041,7.969 ± 7.633,0.423 ± 0.190 and 3.057 ± 2.139) were lower than those of Mo filter(1.915 ± 1.301,12.516 ± 11.632,0.539 ±0.246 and 4.731 ± 3.294),in all the phantoms with different thickness (t values were 4.614,3.209,3.396 and 3.605,P ＜0.05).SNR,CNR,and image score of Rh and Mo group both decreased with the increase of the thickness of all the phantoms.There were no statistical difference (P ＞ 0.05).Conclusions Compared with Mo filter,Rh filter could reduce the radiation dose,and this advantage is more obvious in the thicker phantom when the same image quality is required.

Objective To investigate the readiness for hospital discharge of patients who had undergone spinal operation at West China Hospital, Sichuan University, to guide the medical care and health education. Methods A total of 164 patients after spinal operation who have readying to discharge were selected and investigated by General Information Questionnaire, Activity of Daily Life Scale and Readiness for Hospital Discharge Scale. Apply the Excel for data inputting, SPSS19.0 for statistical analysis and choose rate, x±s and so on for statistical description according to the types of data. Results Of all the 164 patients, 47 cases (28.7%) weren′t well prepared, 117 cases (71.3%) were well prepared;the score of
Readiness for Hospital Discharge Scale was (142.38±34.23 points and the average score of all the articles was (7.12±1.72) points. Conclusions The readiness for hospital discharge score of patients who had undergone a spinal operation at West China Hospital, Sichuan University is low and their readiness for hospital discharge is inadequate. Medical staff can improve the patients′readiness for hospital discharge by strengthening health guidance in order to promote the recovery of patients.

Objective To assess the enhancement characteristic of breast lesions of contrast-enhanced ultrasound (CEUS) in comparison with contrast-enhanced magnetic resonance imaging (MRI).Methods Between August 2011 and March 2013,72 women with 72 lesions were enrolled.All patients underwent ultrasound,CEUS and MRI.The histopathologic results obtained from ultrasound-guided core biopsy or operation excisions were used as the reference standard.CEUS section evaluations were made similar with MRI regarding the size and shape of lesions.Different contrast enhancement patterns including homogeneous/heterogeneous,the tumor areas,the perfusion defect areas,and modality of time-intensity curve were evaluated.Pearson's correlation coefficient,Student's t-tests,and the concordance test were used for evaluation.Results Of the 72 lesions,pathologic examination revealed 56 (77.8％) malignant lesions and 16 (22.2％) benign lesions.The tumor areas measured by CEUS and MRI agreed well,with a correlation of r =0.894,P =0.000.The difference between the two measurements was not significant according to a paired t test (P =0.886).The concordance tests gave a value of the coefficient Kappa =-0.153 (P =0.061),indicating a low concordance between the results obtained with CEUS and those obtained with MRI regarding the enhanced uniformity.There were statistically significant differences in the perfusion defect areas as measured by CEUS and MRI (P =0.01).The CEUS estimates [(0.837 ± 0.827)cm2] were consistently higher than the MRI estimates [(0.576 ± 0.524)cm2].The time-intensity curve patterns between the two groups showed no correlation.Conclusions The enhancement patterns evaluated by CEUS and MRI partly agreed well.There was no direct association between the two methods regarding the enhancement patterns because of the different contrast agent.

Objective To characterize the features of breast mucinous carcinoma on T2WI, dynamic contrast-enhanced MR imaging (DCE-MRI) and diffusion weighted imaging (DWI) and correlate the MR features with histopathology. Methods MR imaging in 8 women (aged 33--65 years old) with mncinous carcinoma of the breast verified histopathologically was performed on 1.5 T scanner with T1WI, FS T2 WI, 3D DCE-MRI using volume imaging for breast assessment(VIBRANT) sequence, DWI with b value of 500 and 1000 s/mm2 by single-shot EPI sequence. According to the lexicon of BI-RADS-MRI designed by ACR, the MR findings were retrospectively studied, including morphology, signal intensity on T1WI and FS T2WI, mass enhancement, the patterns of dynamic enhancement curve, and the mean ADC on DWI. The pathologic diagnosis was made according to the standard criteria by the World Health Organization Classification of Tumors. Results Five patients were pure mucinous carcinoma and 3 patients were the mixed. The amount of mucus ranged from 60% to 90%. All 8 tumors presented as a mass on MRI with low signal intensity on T1WI, high or strongly high signal intensity on FS T2WI and high signal on DWI. The tumor size ranged from 1.3 to 3. 2 cm in diameter. Five tumors showed rim enhancement in early phase and the central part was gradually enhanced in delayed phase. One tumor presented slightly heterogeneous enhancement. Two tumors showed progressively heterogeneous enhancement. The mean ADC values of tumor were (2.41±0.28) ×10-3 mm2/s with b value of 500 s/mm2 and (2.06 ±0. 14) × 10-3 mm2/s with b value of 1000 s/mm2, and higher than that of the normal breast tissue [ ( 1.71 ±0. 32) × 10-3 and ( 1.43 ± 0. 24) × 10-3 mm2/s]. Conclusions The MR features of breast mucinous carcinoma on T1WI, DCE-MRI, and DWI are different from that of invasive ductal carcinomas and may reflect its distinct histological type. The combination of these MR sequences may lead to a pre-operative diagnosis of this tumor.

Surface tension(in terms of hysteresis), lecithin content, PaO2 and lung water content of both bronchial irrigation fluid and lung homogenates were determined after severe inhalation injury in rabbits. In accompany with the decrease of PaO2 and increase of lung water content, there was a remarkable dropping of pulmonary surfactant content as illustrated by progressive increase of surface tension and decrease of lecithin content in both bronchial irrigation fluid and lung homogenate. It was proposed that dropping of pulmonary surfactant played an important role in the pathogenesis of pulmonary edema after inhalation injury. Therefore, successive determinations of lecithin content or and surface tension of bronchial irrigation fluid might serve as an early diagnosis as well as a pro-gnostis aid in severe inhalation injuries.

Objective To evaluate the value and limitation of Gd-DTPA enhanced MRI for neuroblastoma staging in children. Methods Twelve cases of neuroblastoma proved by operation or bone marrow aspiration were examined by gadolinium-enhanced MRI. The age ranged from seven months to five years, mean 3.7 years. Eight tumors originated from adrenal, and four from posterior mediastinum. Conventional sequences, double dose gadolinium-enhanced MRI, and 3D CEMRA were used in all patients. Six cases were examined by CT in same time. Imaging staging on surgic-histopathological-based International Neuroblastoma Staging System (INSS) was performed. Results Six patients were staged by CT, including stage Ⅰ-Ⅱ in 2 cases, stage Ⅲ in 4 cases, and stage Ⅳ in none. Twelve patients were staged by conventional MRI, including stage Ⅰ-Ⅱ in 2 cases, stage Ⅲ in 9 cases, and stage Ⅳ in 1 case. Twelve patients were staged by double dose gadolinium-enhanced MRI, including stage Ⅰ-Ⅱ in 1 case, stage Ⅲ in 1 case, and stage Ⅳ in 10 cases. Conclusion Gadolinium-enhanced MRI was a single best imaging modality for neuroblastoma, most useful for distal to diaphragm metastasis, dumbbell tumor intraspinal extension, and bone marrow metastasis that was not detected by aspirate examination. Enhancement MRI was important in evaluating the therapy and was also helpful in assessing the therapeutic efficacy and relapse. 3D CEMRA helps demonstrate large vascular encasement and tumor erosion into important organs, and it is useful in assessing the respectability. Long examination time and lack in showing the characteristic calcium were the limitations.