A thin layer chromatograph-fluorescent method was used to assay content of scopoletin in Erycibe obtusi folia Benth.. The method showed that it not only can minimize analytical errors in thin layer scan and column contamination in HPLC,but also eliminated mutual interference of components in the preparation. It provides a good assay technique for quality control of hebal preparations. The method was carried out on thin layer plates of silica gel H(40?)with benzene-ether (1. 1: 1 )as the developer,at an excitation spectrum of 347nm,fluorescence spectrum of 420nm and a temperture of 25??1℃.The method showed good linearity(r= 0. 9998, n = 5)in the range of 0.08~0. 40?g/?l of scopoletin and the average recovery was 98. 52 % ~ 102. 67 % with RSD

Objective To study the clinical effects of postpartum haemorrhage and the clinical effects of psychosocial intervention. Methods Choose 88 cases, patients treated in our hospital, it can be divided into two groups, respectively using oxytocin combined conventional nursing mother, yan pei combined psychological intervention treatment, analysis its therapeutic effect. Results The curative effect of observation group of patients, postpartum haemorrhage amount and quality of life, each index significantly better than the control group, the changes of its data through comparison between groups, differences, statistically significant(P<0.05). Conclusion The patients with postpartum haemorrhage, with the treatment of the psychological intervention and the treatment of the psychological intervention, the effect is good.

lymorphism of CD14 gene is a risk factor for diabetic nephropathy.

Objective To investigate the effect of berberine on the radiosensitivity of cervical cancer cells.Methods 5,10,15,20 μmol/L of berberine were used to treat cervical cancer cell lines of Siha,HeLa,Caski.DMSO was applied as control of drug treatment.Cell proliferation was detected by the CCK-8 method,and then the half inhibitory concentration of berberine was calculated.Cell apoptosis and cell cycle distribution were detected by flow cytometry.Protein expressions of Cleaved Caspase-3,Cyclin B1,CDK1,STAT3 and p-STAT3 were detected by Western blot.Cervical cancer cells of Siha were treated by berberine with a half inhibitory concentration for 24 h and then irradiated with 0,2,4,6,8 Gy of X-rays.Cell clone assay was used to detect cell survival.Results Berberine could inhibit the growth of cervical cancer cells with a half inhibition concentration of(16.84 ± 3.52),(23.54 ± 8.67),(21.86 ± 6.35)μmol/L for Siha,Caski,and HeLa cells,respectively.The berberine at 17 μmol/L could induce apoptosis (t =56.847,P ＜ 0.01) and G2/M phase arrest (t =47.251,P ＜ 0.01) in Siha cells,which also inhibited the expressions of Cyclin B1,CDK1 and p-STAT3 and promoted the expression of cleaved Caspase-3,but did not influence the expression of STAT3 in cervical cancer cells.Treatment of cells with 17 μmol/L berberine increased the radiosensitivity of cervical cancer cells with a sensitivity enhancement ratio of 1.55.Conclusions Berberine can inhibit cell proliferation,promote apoptosis,block cell cycle,and increase radiosensitivity of cervical cancer cells.

OBJECTIVESTo explore the significance of noninvasive central blood pressure detection in blood pressure management and vessel function assessment of hypertension.

METHODSA total of 348 subjects were recruited in the study and A-pulse CASP software were derived to measure the parameters of CASP and vessel function. The recruited subjects were divided into different groups for analysis according to age and disease.

RESULTS(1) CASP in hypertension group were both higher than those in normal group, while RAI and PRT lower. (2) Hypertensive patients of which brachial blood pressure were controlled have lower CASP and RAI than those above the level. (3) Hypertensive patients whose brachial blood pressure were controlled have significantly higher CASP than normal subjects.

CONCLUSIONSCentral blood pressure is more reflective of actural blood pressure and vessel function. Enhanced control of CASP in hypertensive patients contributes to improving vessel compliance.

Objective To study the efficacy of entacapone for motor fluctuations in Parkinson's disease. Methods Pubmed, Embase, Cochrane Database were searched for randomized controlled trial studies (RCTs) on entacapone compared to placebo in the treatment of Parkinson' s disease with motor fluctuations. Data were extracted and evaluated by two reviewers independently with designed extractian form. Revman 4. 2. 10 software was used for data analysis. Results Ten RCTs involving 2212 patients were included. The Results showed that entaeapone tends to increase "on" time, decrease "off" time and daily levodopa dosage (WMD=-1.41,95% CI -2. 09- -0.72) and improve "on" time motor symptoms and the quality of life. But it might increase the rate of dyskinesia (OR=2.00, 95% CI 1.55-2.58).Conclusion The systematic review indicates that entaeapone might be an effective agent for parkinsonian patients with motor fluctuations and more high quality RCTs with the same effect measures are required.

Objective To systemically evaluate the therapeutic effect of pramipexole in treating Parkinson's disease. Methods Pubmed, Embase, Cochrane Database were used to search for randomized control trials (RCTs) on Parkinson's disease treated with pramipexole. Meticulous data wre extracted and meta-analysis was performed according to a preset protocol. Results Ten RCTs involving 1738 patients were included. The results of meta-analysis showed that pramipexole decreased the total scores of unified Parkinson's disease rating scale [UPDRS, WMD=-10.01,95%CI (-12.76,-7.26)],UPDRS part [WMD=-2.44,95%CI(-2.93,-1.95)],and UPDRS partⅢ[WMD=-6.61,95%CI(-8.38,-4.84)]. Pramipexole also reduced the score of UPDRS part Ⅳ[WMD=-0.73,95%CI(-1.16,-0.30)] in advanced patients(P＜0.05＝.Three studies referred to the effect of pramipexole in treating parkinsonian tremor. However there was a clinical heterogeneity among the studies, and two of them revealed a significant difference. Conclusions Pramipexole might improve motor symptoms and the quality of life in advanced patients, as well as improve tremor, which needs more RCTs to be confirmed.

Objective To investigate the effect of persistent hypoglycemia on the cerebral oxidative stress and matrix metalloproteinase-2 (MMP-2) activity of newborn rats and to explore the potential mechanism of hypoglycemia-induced brain injury of newborn.MethodsSeven-day-old rats were injected insulin subcutaneously to induce persistent hypoglycemia.Cerebral cortex,hippocampus and thalamus were collected after 36 hours' observation and stored in -80 ℃.Glutathione (GSH)and glutathione disulfide (GSSG) were detected by commercial kit and GSSG/GSH was calculated.MMP-2 activity was detected by gelatin zymography. The data were analyzed by t test,one or two-wayanalysis of variance and Pearson correlation analysis.Six rats were set as the normal glucose group. ResultsThirty-six hours after induction of hypoglycemia, the cerebral GSSG and GSSG/GSH of hypoglycemic pups elevated about 1.5 times higher than those of control group [GSSG:(15.89 ± 5.46) vs (6.15 ± 3.42) mg/g protein,t =3.704,P =0.004; GSSG/GSH:(5.58± 1.79) ％ vs (2.79±1.76) ％,t =2.712,P=0.022].The GSSG and GSSG/GSH in thalamus of hypoglycemia were significantly higher than those of control [GSSG:(15.93 ± 5.75) mg/g protein vs (5.03±5.14)mg/g protein,P＜0.05; GSSG/GSH:(6.50±3.25) ％ vs (2.41±3.12) ％,P＜0.05],whereas there were no significant differences in the cortex ard hippocampus.The total MMP-2 activity of hypoglycemic animals (2.22±0.59) was significantly higher than that of control (1.21± 0.17)(t=4.064,P=0.002),and significant differences were found between the two groups in cortex (2.14 ± 0.5 vs 1.17± 0.27),hippocampus (2.31± 0.72 vs 1.22 ± 0.37) and thalamus (2.22±0.68 vs 1.24±0.18) with all P＜0.01].The activity of MMP-2 was positively related to GSSG (r=0.575,P=0.0002) and GSSG/GSH (r=0.484,P=0.0003).ConclusionsOxidative stress might play an important role in the persistent hypoglycemia induced brain injury of 7-day-old rat pups and thalamus might be most vulnerable to hypoglycemia.Hypoglycemia might also elevate MMP-2 activity which is positively related to GSSG level and GSSG/GSH of the brain.

Objective To study the effect of assessment criteria for thoracic surgery health education in the health education to patients undergoing thoracic surgeries.Methods One hundred and forty patients undergoing selective surgeries were evenly divided by their registration sequence into the control and experiment groups:The former received routine health education and the latter the health education based on thoracic surgery health education criteria.The effects of the health education were assessed by self-designed recognition evaluation criteria for thoracic surgery health education.Results The experiment group was significantly better than the control in terms of health-related knowledge,health belief and health behaviors,except operative approach,anesthesia method and intraoperative medication(all P<0.05).Conclusions The assessment criteria for thoracic surgery health education are helpful for the nurses to perform health education purposefully and pertinently.The criteria are also applicable clinically for the nursing administers to carry out health education assessment.

Objective To establish a reference range of cancellous bone ultrasonic backscatter coefficient (BSC) in term neonates,and to determine the significance of BSC in bone nutritional status in term newborns and infants.Methods Six hundred and sixty-five term newborns,who were born in Shanghai First Maternity and Infant Hospital from July 2014 to August 2014,were enrolled.On the third day and the 42nd day after birth,all infants underwent ultrasonic measurement of the calcaneum bone using a broadband-non-focus ultrasonic transducer at three frequencies (2.25,3.5 and 5 MHz).MATLAB software was used to analyze the backscatter signals.Clinical data of the infants,including gestational age,birth weight and birth age,were recorded at the same time.Statistical analyses of the data included variance analysis,Tukey's test,rank sum test,Dunn test and Pearson or Spearman correlation analysis.Results (1) Of the 665 infants,335 were male.The mean gestational age at birth was (38.0± 1.9) weeks,and the mean birth weight was (3 1524±226) g.(2) On the third day,there were significant differences in BSC values acquired at 2.25 and 3.5 MHz among the groups with different gestational ages or birth weights (2.25 MHz:H=9.842 and 17.271;3.5 MHz:H=6.275 and 21.450,respectively,all P＜0.05).At 3.5 and 2.25 MHz,BSC values were positively correlated with gestational age (r=0.047 and 0.035,respectively,both P＜0.05) and birth weight (r=0.125 and 0.186,both P＜0.05).(3) On the 42nd day,significant differences in BSC were observed at each frequency among the groups with different gestational ages or birth weights (gestational age:H=76.832,16.498 and 32.756;birth weight:H=70.014,18.095 and 34.126;all P＜0.01).Gestational age and birth weight were positively correlated with BSC value acquired at each frequency (r=0.397,0.286 and 0.272 for gestational age,0.451,0.223 and 0.196 for birth weight;all P＜0.01).Conclusions A preliminary BSC reference percentile in term-born infants is established.There are positive correlations between BSC value and newborn gestational age and birth weight at certain frequencies,which can reflect the status of the newborn's cancellous bone.Determination of BSC value should be performed during the follow-up period to assess the nutritional status of neonatal bone.