Objective To obtain stable-expressing cell lines which could constantly express tropic1808 gene and investigate the effect of tropic1808 by detecting the expression level of NF-H in PC12 cells. Methods The recombinant tropic1808 plasmid was constructed and transfected into PC12 cells.PC12 cells stable-expressing tropic1808 were established by G418 selection and confirmed by Western blot.The expression of NF-H in the PC12 stable-expressing tropic1808 was observed by RT-PCR,real time PCR,Western blot analysis and cytohistochemistry methods. Results Western blot confirmed that tropic1808 gene could express stably in PC12 cells.The data of RT-PCR,real time PCR,Western blot and cytohistochemistry all demonstrated that the expression of NF-H was increased in PC12 cells stable-expressing tropic1808.Conclusion PC12 cell lines stable-expressing tropic1808 were obtained and the expression of NF-H up-regulated in the PC12 cells stable-expressing tropic1808 gene.

Cell biology is an important course for all the students of medical school,and this course should be given with different content for the students with different academic background.The major content of cell biology course for the freshmen should be the cellular structure and the sub-cellular structure,and for the senior undergraduates,the content should include molecular basis of the cell function,and for the postgraduates,this course should focused on the molecular mechanisms of the cell function.To teach students in accordance of their background and aptitude is a good way to get a satisfied effect in a course for both teachers and students.

Anticoagulants ; therapeutic use ; Child, Preschool ; Fontan Procedure ; adverse effects ; Heparin ; therapeutic use ; Humans ; Male ; Protein-Losing Enteropathies ; drug therapy ; etiology

Proteome is defined as all proteins expressed in a cell, a tissue or an organism. Proteomics, a science of understanding the life activity law at the whole protein level, takes proteome as a research object; it is also a scientific method for quantitative detection of one or more protein populations or proteomes in a cell or tissue. Cerebral ischemic preconditioning has been one of the research hotspots in the field of neuroscience for decades. Its neuroprotec-five mechanism is associated with the changes of protein expression levels after cerebral ischemia, Therefore, proteomics has become more and more important in its mechanism study. This article mainly presents the major techniques of proteomics and their application in the studies of cerebral ischemic preconditioning.

Objective to compare the calculated effective radiation dose variations in cardiac interventional procedures of coronary angiography (CAG) and percutaneous transluminal coronary angioplasty (PCI) by using the different tissue weighting factors recommended by ICRP Publication 60 with that recommended by ICRP Publication 103. Methods Measurements of organ doses were carried out with an anthropomorphic phantom equipped with thermoluminecent dosimetry. The effective radiation doses were separately calculated using the tissue weighting factors provided by ICRP 60 and provided by ICRP 103, and the results obtained from ICRP 60 were compared with that obtained from ICRP 103. Conversion factors of effective, radiation dose to dose area product (DAP) were also calculated according to different publication.Results The variations in the effective dose were 6.88% for CAG and 8.46% for PCI. The effective dose variations in CAG and PCI were. 7.25% and 8.76% for female and were 6.51% and 8.17% for male,respectively. The effective conversion factors of DAP were. 0.10 ～ 0.11 and 0.13 ～ 0.14 for CAG and PCI,respectively, according to ICRP 60 and 103. Conclusion The variation of conversion factors of effective dose indicates that in making estimation of effective radiation dose. by using the widely accepted conversion factors, the adjustment of organ weighting factors recommended by ICRP 103 should be considered.

Myocardial infarction (MI) is a major cause of death and disability worldwide.The definition of myocardial infarction has been updated.Released in 2012,Third Universal Definition of Myocardial Infarction of ESC/ACCF/AHA/WHF Expert Consensus,made the clinical application value of troponin clear and suggested to monitor the dynamic changes of cTn in patients with suspected,emphasized the importance of clinical differential diagnosis.In consensus,it also noted that the application of high sensitive troponin can lead to false positives results,and pointeds out that in the assessment of cardiac injury associated with percutaneous or surgical coronary procedures,the rational cut-off value of cTn remains controversival.In addition to acute coronary syndrome,cTn elevations are common in many disease states,and with the improvement of detection sensitivity,low level increase of cTn,will be more.In acute and chronic heart failure,the cTn is elevated,and ACS may be the cause of worsening heart failure,So we should identify whether the cTn elevation is pure from the myocardial injury of heart failure or myocardial necrosis caused by ACS.Nowadays,the increased incidence of myocardial infarction has much to do with the wide application of cTn.Clinical applications of cardiac biomarkers and imaging techniques are flexible as the differences in the diagnostic techniques of myocardial infarction among countries and regions result.

Objective To evaluate the effect of a new schistosomiasis control strategy based on the infection source control in four-lake regions of Hubei Province. Methods The new strategy based on the infection source control included the safe water sup-ply,feces harmless treatment,replacing cattle with machine,captive livestock,chemotherapy,Oncomelania hupensis snail con-trol in susceptible areas,hardening ditches,etc,and the new control strategy was implemented for 7 years in 9 counties(cities or districts)of the four-lake regions. The schistosomiasis situations were investigated before and after the intervention of the new strat-egy. Results The coverage rates of feces harmless toilets(three format toilets),biogas digesters,and safe water supply were 42.00%,23.16% and 93.76%,respectively. The number of ditch hardening was 1 960;the area of eliminating snails was 1 378.42 hm2;the number of persons who received the health education was 3 524 818 accounting for 92.17%of the total popula-tion;the number of person-time of taking active protection measures was 516 636 963. The average annual decline rates of schisto-some infection were 24.42%and 38.38%in residents and cattle,respectively. The endemic villages decreased by 4.77%,the vil-lages with snails decreased by 7.05%,and the villages with snails accounted between 63.19%and 66.21%of the total endemic vil-lages. The area with snails decreased by 90.35%,and the advance schistosomiasis patients decreased by 5.55%,and the fatality rate of advanced schistosomiasis patients decreased by 1.24%. No acute schistosomiasis patients occurred. Type One and Two en-demic(heavy endemic)villages decreased to zero;Type Three endemic(moderate endemic)villages decreased by 10.22%;but Type Four endemic(mild endemic)villages increased by 66.38%;and Type Five endemic(no endemic for 5 years)villages de-creased by 0.22%. The standards of schistosomiasis transmission controlled have achieved on schedule. Conclusion The imple-mentation of the new schistosomiasis control strategy based on the infection source control in four-lake regions of Hubei Province can effectively control schistosomiasis.

Objective To establish a high sensitive spectrophotometry for determination of trace cadmium in the water. Methods A complicated ion-association complex of Cd(Ⅱ)-potassium iodide-malachite green was formed in the phosphate acid, and the addition of gelatine could enhance the sensitivity of the reaction.The maximum absorption of the ion-association complex was at 680 nm,the effect of experimental conditions such as the reagents concentration,the temperature and the influence of foreign matters were considered.Results In the optimum condition(6.0 ml of 40% potassium iodide-aseorbic acid solution,0.5 ml of 5.0 mol/L phosphate acid solution,0.5 ml of 0.5% gelatine solution,1.5 ml of 1.0?10~(-3)mol/L malachite green solution in a 25ml volumetric flask,diluted with water and mixed well and determined immediately),the linear regression equation was △A=0.011+ 0.957 c,r=0.998 5.Beer's law was obeyed in the range of 0.02 ?g/ml to 0.80 ?g/ml for Cd(Ⅱ)and the limit detection was 0.02 ?g/ ml.The composing ratio of the complex was MG:Cd:I=2:1:4,and its apparent molar absorptivity coefficient was 1.08?10~5 L/(mol? cm).The recovery rates of Cd(Ⅱ)were 97.0%-101.5%,RSDs were 1.36%-3.58%.Conclusion This method is sensitive,simple, rapid and is applicable to the determination of the trace Cd(Ⅱ)in water.

OBJECTIVE:To observe long-term efficacy of intensive rosuvastatin therapy in the treatment of acute coronary syn-drome (ACS) and explore its possible mechanism,so as to provide reference for clinical diagnosis and treatment. METHODS:ACS patients receiving rosuvastatin after cured in our hospital during Jan. 2013-Jan. 2015 were divided into observation group(55 cases)and control group(47 cases)according to the dose of rosuvastatin. Under same guidance of physicans,2 groups were addi-tionally given Rosuvastatin calcium tablets(20 mg for observation group,10 mg for control group,qd,po)orally on the basis of routine treatment. Both groups were treated for 1 year. The incidence of 1-year accumulative main adverse cardiac event (MACE) were compared between 2 groups as well as the levels of blood lipid and serum cytokines,the occurrence of ADR before treat-ment,after 3 months of treatment. RESULTS:The incidence of accumulative MACE in observation group was significantly lower than control group,with statistical significance (P<0.05). After treatment,the levels of TC and LDL-C in 2 groups were de-creased significantly;the level of LDL-C was inCreased significantly compared to before treatment,and the observation group was significantly lower than the control group,with statistical significance(P<0.05). The levels of IL-1β and TNF-α in 2 groups were decreased significantly (P<0.05);the observation group was significantly lower than the control group,with statistical signifi-cance (P<0.05). The peripheral blood LDL-C was positively correlated with IL-1β and TNF-α in observation group after treat-ment,with statistical significance(P<0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:Intensive rosuvastatin therapy can significantly improve the long-term prognosis for ACS patients,which may associate with lipid metabolic regulation and anti-inflammatory effect.

OBJECTIVE:To explore the significance of clinical pharmacists providing pharmaceutical care for pregnant patients with asthma in respiratory department. METHODS:Clinical pharmacists participated in the analysis of 2 cases of pregnancy complicating with asthma and applied pharmaceutical suggestion and therapy plan as penicillin anti-infective thera-py,intravenous dripping of magnesium sulfate combined with doxofylline,albuterol aerosol treatment instead. RESULTS:The asthma symptom had been relieved significantly and then the patients were discharged from the hospital after clinical phar-macists disposed the symptom appropriately. CONCLUSIONS:The participation of clinical pharmacists in pharmaceutical care for pregnant patients with asthma in respiratory department. Can improve the prognosis effectively and guarantee the safety of drug use.