Objective To develop and verify a qPCR method for the qualitative and quantitative analysis of residual host DNA in human rabies vaccine(Vero cells) stock solution.Methods The qPCR standard curve was established by using the Vero cell DNA quantitative national standard,and the residual host DNA was extracted using magnetic beads.The specificity,repeatability,intermediate precision,accuracy and durability of the method were verified,and the linear range and limit of quantification were determined.The residual DNA of three batches of human rabies vaccine(Vero cells) stock solution was quantitatively analyzed and the fragment size was qualitatively analyzed by using this method.Results The correlation coefficients(R~2) of Vero cell DNA quantitative national standard amplification standard curve were all more than 0.99 by qPCR,and the quantitative range was 0.3 pg/mL～30 ng/mL.The method showed good specificity and repeatability.In the verification of intermediate precision,accuracy and durability,the relative standard deviations(RSD)of detection results of the samples were all less than 10%.The residual DNA content of Vero cells in three batches of stock solution was 0.20～0.77 ng/dose,which met the relevant standard of Chinese Pharmacopoeia(Volume Ⅲ,2020edition).The residual DNA fragments greater than 154 bp accounted for 52%～63%.Conclusion The developed qPCR method for the detection of residual DNA in human rabies vaccine(Vero cells) stock solution had good specificity,repeatability,intermediate precision and durability,and qualitatively and quantitatively analyzed the residual DNA rapidly and accurately,which was of great significance for improving the detection and control of residual DNA content in the production process and final product of human rabies vaccine(Vero cells).

Objective To explore the experience of surgical treatment for appendiceal carcinoid.MethodsThe clinical data of 12 appendiceal carcinoid cases received surgery were retrospectively studied.ResultsPreoperatively,all the 12 cases were considered as appendicitis.The definite diagnosis was verified by pathologic study postoperatively.Among the 12 cases, tumors less than 1 cm 9 cases, between 1 cm to 2cm 2cases, but without serosal involvment or lymph node metastasis(2 cases) recieved simple appendectomy.One case with a tumor larger than 2cm and serosal involvment underwent fight hemicolectomy.ConclusionOwing to its lack of specific symptoms,the diagnosis of appendiceal carcinoid was usually reached after operation.The choice of surgical procedures should based on the size and depth of invasion of the tumor and lymph node metastasis status.

Objective The aim of this study was to explore the effects of volume controlled ventilation (VCV),pressure controlled ventilation(PCV)and pressure controlled ventilation-volume guaranteed (PCV-VG)on respiration and circulation in elderly patients undergoing thoracic surgery. Methods Thirty-six elderly patients who underwent thoracic surgery were enrolled in our study. Patients were divided into VCV,PCV and PCV-VG groups according to randomized design. The hemodynamic and respiratory data and the arterial blood gases had been recorded in the pre-operation,20 min,40 min,60 min after OLV and 20 min after the resumption of two lung ventilation. Results Compared with VCV group,Ppeak value was significantly lower in PCV and PCV-VG groups(P < 0.05),and the difference was not found between the PCV and PCV-VG groups. PaO2 levels in PCV and PCV-VG groups were higher than that in VCV group after the point of OLV+40(P < 0.05). Comparison of PCV group,PaO2 in PCV-VG group was higher,but did not show a significantly improved during OLV(P > 0.05). Conclusion Compared with VCV,the use of PCV and PCV-VG have significant advantages in the operative oxygenation and airway pressure for elderly patients undergoing OLV.

The nose has a three-dimensional structure located in the centre of the face, and plays an important role in the facial aesthetics. The anatomical structure of the nose, especially the three components-nasal cartilages, muscles, and ligaments are spatially connected, which interact with each other, affecting the size, position, shape, and function of the nasal tip. The author attempts to review the literature on the spatial arrangement and relationship between the nasal cartilage, muscle, and ligament from the anatomical and functional perspective, and summarize their effects on the position, shape, and function of the nasal tip. The purpose is to explore how to bionically adjust and transplant the spatial arrangement of the cartilage, ligament and other subunit structures of the nasal tip without affecting the inherent structure and function of the nose, and to seek the theoretical basis for the establishment of the nasal tip shaping technology in line with the anatomical and physiological functions.

The nose has a three-dimensional structure located in the centre of the face, and plays an important role in the facial aesthetics. The anatomical structure of the nose, especially the three components-nasal cartilages, muscles, and ligaments are spatially connected, which interact with each other, affecting the size, position, shape, and function of the nasal tip. The author attempts to review the literature on the spatial arrangement and relationship between the nasal cartilage, muscle, and ligament from the anatomical and functional perspective, and summarize their effects on the position, shape, and function of the nasal tip. The purpose is to explore how to bionically adjust and transplant the spatial arrangement of the cartilage, ligament and other subunit structures of the nasal tip without affecting the inherent structure and function of the nose, and to seek the theoretical basis for the establishment of the nasal tip shaping technology in line with the anatomical and physiological functions.

The nose has a three-dimensional structure located in the centre of the face, and plays an important role in the facial aesthetics. The anatomical structure of the nose, especially the three components-nasal cartilages, muscles, and ligaments are spatially connected, which interact with each other, affecting the size, position, shape, and function of the nasal tip. The author attempts to review the literature on the spatial arrangement and relationship between the nasal cartilage, muscle, and ligament from the anatomical and functional perspective, and summarize their effects on the position, shape, and function of the nasal tip. The purpose is to explore how to bionically adjust and transplant the spatial arrangement of the cartilage, ligament and other subunit structures of the nasal tip without affecting the inherent structure and function of the nose, and to seek the theoretical basis for the establishment of the nasal tip shaping technology in line with the anatomical and physiological functions.

The nose has a three-dimensional structure located in the centre of the face, and plays an important role in the facial aesthetics. The anatomical structure of the nose, especially the three components-nasal cartilages, muscles, and ligaments are spatially connected, which interact with each other, affecting the size, position, shape, and function of the nasal tip. The author attempts to review the literature on the spatial arrangement and relationship between the nasal cartilage, muscle, and ligament from the anatomical and functional perspective, and summarize their effects on the position, shape, and function of the nasal tip. The purpose is to explore how to bionically adjust and transplant the spatial arrangement of the cartilage, ligament and other subunit structures of the nasal tip without affecting the inherent structure and function of the nose, and to seek the theoretical basis for the establishment of the nasal tip shaping technology in line with the anatomical and physiological functions.

Obiective To observe the clinical effect of treating upper respiratory infection induced coughing with atomizated ioint of compound liquorice oral solution and ipratropium bromide.Methods 69 cases of respiratory tract infection induced coughing were randomly recruited into A,B,and C group.Group A was treated with atomizated joint of compound liquorice oral solution and ipratropium bromide;Group C was treated with azithromycin and levofloxaein;Group B was treated with the combined therapy of Group A and Group C.Clinical effects and side effects were observed after the treatment.Results Symptom scores of cough in group A and group B were lower than those in group C.The difference was statistically significant(P＜0.05).Dry mouth and throat complaints disappeared in group A and group A and group B after the treatment.Conclusion Tomizated ioint of compound liquorice oral solution and ipratropium bromide had good therapeutic effects in treating cough induced by upper respiratory infection.

Objective To improve the protective strategy of occupational risks for technicians in blood centers so as to ensure their health and safety.Methods The occupational risk factors were analyzed by the 5W1H method,and the corresponding protective measures were introduced.Then,the protective measures were verified dynamically by the PDCA circulation,and were standardized and further spread.The projects with poor effect were replanned and entered the PDCA recycling.Results The system of the 5W1H method combined with PDCA circulation could identify and control various occupational risks,and effectively prevent the occupational detriments.Condusion Combined application of the 5W1H method with PDCA circulation is an effective way to protect the health and safety of technicians in blood centers and an effective mode to improve the management of occupational risks.

Objective To discuss the perioperative nursing points of vitreoretinal surgery under tempo-rary kerstoprosthesis combined with penetrating keratoplasty for treatment of complex ocular trauma and anteri-or and posterior ocular segment lesion. Methods Clinical data of 21 patients (21 eyes) undergoing vitreo-retinal surgery under artificial cornea combined with penetrating keratoplasty were analyzed, perioperative nursing problems were evaluated and corresponding nursing measures were adopted. Results Visual acuity of 16 eyes were greatly improved,other 5 eyes showed no amelioration.By effective nursing mea-sures, all nursing problems were settled. Conclusions Although the functional outcome of a combined procedure is limited,it contributes to preserve vision of patients. Good periopertive nursing is pivotal for successfal operation.