Objective: To understand the current status of health education faculty in Yuzhong District of Chongqing, so as to explore the implementation path to improve the construction of health education faculty. Methods: In August 2023, stratified random sampling was used to directly select a total of 555 teachers and school doctors from 17 schools in 11 streets of Yuzhong District, who were invited to conduct a questionnaire survey. A total of 409 fulltime or parttime health education teachers were included in the analysis of the results. Both χ2 test and Fisher exact probability method were analyzed for compare the differences in the frequency of conducting health education courses and specializing in specializing areas among different plant health education teachers in Yuzhong District. Results: Health education course faculty were mainly female teachers, accounting for 81.66%. The proportions of physical education (PE) teachers, school doctors, head teachers and teachers from other disciplines were 14.67%, 8.31%, 62.84%, 14.18%, and the class teachers (81.33%) and school doctor (80.95%) were found of the highest proportion of providing health education courses, and the teaching section was mainly elementary school (first to third grade: 42.30%, fourth to sixth grade: 44.74%). Among the teachers, school doctors had the highest proportion (88.24%) of receiving training related to health education courses. And there was a significant stastical difference in the frequency of conducting health education classes among PE teachers, school doctor, head teachers and the teachers of other courses (χ2=54.46, P<0.01). The results of Fisher exact probability method showed that the differences in selfassessment of the most and least skilled areas among PE teachers, school doctor, head teachers and teachers of other courses were statistically significant (P<0.01). Conclusions Health education faculty in Yuzhong District is mainly composed of PE teachers, school doctor, head teachers and teachers of other courses. The standardized training and assessment of teaching for school doctor could promote the school nurse group developing into specialized health education faculty.

OBJECTIVETo evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases.

METHODSVero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells.

RESULTSFive pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect.

CONCLUSIONsiRNA may be effective in inhibiting SARS-associated coronavirus replication.

Animals ; Cercopithecus aethiops ; RNA, Small Interfering ; pharmacology ; SARS Virus ; drug effects ; Transfection ; Vero Cells ; Virus Replication ; drug effects