A method of preparation of Chondroitin su(?)fate from Raja porosa cartilage was presented, using the extraction process of increasing concentration of strong bases, the hydrolysis of complex enzyme and the alcohol precipitat(?)on method. The technologic condition was researched. The white powder of Chondroitin sulfate was obtained at the yield rate of 2.82% and the purity of 93.6%.

The pathogenesis of diabetic retinopathy (DR) is more complex. For the upstream of traditional pathogenesis, to looking for unifying mechanism theory which proposed in foundation of common promoters and the latest view of DR may be the result of chronic inflammation. Both of them provide the basic and clinical theraby of DR with new direction. Therefore, there are many related issuess till needs to intensive study.

Objective To explore the relationship between the expression of E-cadherin catenin complex(CCC) and the invasiveness of pituitary adenoma.Methods The expession of E-cadherin catenin complex was determined by immunohistochemistry in 45 cases of human pituitary adenomas by random sampling,including invasive adenoma 27 cases,noninvasive adenoma 18 cases.The relativity of their expression with hormone-producing,pituitary apoplexy and necrosis or cystoid change,tumor diameter was analyzed.Results The invasive group had a significantly lower expression of E-cad and ?-cat than that of noninvasive group(?2=8.551,P0.05).Moreover,the expression of E-cad was significantly lower in endocrine inactive group than that in endocrine active group(?2 =5.020,P

AIM To study the effect of Topotecan(TPT) on cell cycle and apoptosis of hunman lung cancer SPC A 1 cells and the role of Caspase 3 and bcl 2 involved in TPT induced apoptosis. METHODS Cells were incubated with TPT(5,10,15 and 20 mg?L -1 ) for 24 h. Apoptosis was detected by TUNEL staining, electronic microscope and DNA agarose gel electropheresis. Flow cytometer was used to analyse cell cycle changes and to detect apoptotic rates and bcl 2 expression of cells treated with TPT or TPT and 50 ?mol?L -1 Ac DEVD CHO(specific Caspase 3 inhibitor). RESULTS Apoptosis of cells treated with TPT(5 mg?L -1 ) was not detected, but apoptosis rates increased with the increase of TPT concentration and there was significant difference between each concentration group( P

Objective:To investigate the association between anxiety and the change of humoral immune functions and its correlation with HLA-DQB1 polymorphisms.Methods:Total 31 resident doctors were selected randomly and tested by State-Trait Anxiety Inventory(STAI).IgG,IgA,IgM,complement C3 and complement C4 were detected with BECKMAN array360 system;HLA-DQB1*02、*03、*04、*05 and*06 alleles were individually amplified by polymerase chain reaction(PCR)using exon2 group-specific primers.The correlation between immune function and HLA-DQB1 polgmorphisms were investigated.Results:Statistical analysis showed that there was positive correlation with State Anxiety (Ta) and complement C3,either Trait Anxiety (Tc) and complement C3.There was significant difference between HLA-DQB1*02 positive and negative in Ta (P＜0.05),while no difference in complement C3(P＞0.05).There was significant difference between HLA-DQB1*04 positive and negative in Ta and Tc(P＜0.05),while no difference in complement C3(P＞0.05).Conclusion:Anxiety could change some humoral immune functions and this is related with HLA-DQB1 polymorphism.

Local recurrence and distant metastasis is the major factor of impacting long-term survival for patients with esophageal carcinoma after radical resection. Postoperative radiotherapy and chemotherapy is expected to reduce the rate of local recurrence and distant metastasis, but views for the value of postoperative radiochemotherapy were inconsistent in the past. In recent years, scholars have performed much more clinical studies on postoperative radiotherapy and chemotherapy and found that the combined postoperative radiotherapy and chemotherapy after surgery significantly reduces the recurrence rate and distant metastasis rate and can enhance the survival of the patients.

Objective To investigate the effect of continuous blood purification(CBP) in the patients with severe acute pancreatitis(SAP). Methods Twenty-six patients with severe acute pancreatitis were divided into the control group and the observation group randomly. Fourteen cases in the control group were given conventional treatment only, 12 cases in CBP group were given continuous blood purification as well as the conventional treatment. The APACHE Ⅱ score and CRP of pre- and intra-treatment at the 2nd and 5th day were observed in the two groups. The duration of mechanical ventilation, the duration of ICU stay, the mortality of hospital were observed as well. Results The APACHE Ⅱ score and CRP of the second day and the fifth day of intra-treatment in the control group were 1.61±3.00,10.76±4.30, (149.71±60.06) mg/L and (101.21±33.98) mg/L, respectively, which were significantly different to those of (8.20±1.87) mg/L, (6.19±1.49) mg/L, (109.17±23.91) mg/L,(70.83±25.45)mg/L in the CBP group(P＜0.05). The duration of mechanical ventilation,the duration of ICU stay and the mortality of hospital were (13.29 ± 6.23) d, (16.00 ± 6.86) d, 21.43% in the control group, which were significantly lower than those of (8.00 ±5.27)d,(10.33 ±4.81)d,8.33% in the other group (P ＜0.05).Conclusions CBP is an effective method for the patients with severe acute pancreatitis to shorten the duration of mechanical ventilation and the ICU stay,and decrease the mortality of hospital.

In the hospital administration,the establishment and the implementation of the strategic planning of the hospital occupies a very important position.This paper,combined with the actual situation of a children's hospital in Chongqing,describes the hospital rational use of three level management system of the hospital,office,department,in order to get the full investigation of the national macro policy,the development of the medical industry and the status of the hospital and other aspects,and then supports efficient workflow to complete the hospital strategic planning.This paper also focuses on the target implementation,program implementation and project implementation of three levels,in order to illustrate the process for the implementation of the strategy to provide a reference of.hospital strategic planning formulation and implementation.

Objective To evaluate the effect of sevoflurane preconditioning on autophagy during ischemiareperfusion (I/R) injury to isolated rat hearts and the role of PI3K/Akt signaling pathway.Methods Healthy adult male Wistar rats,aged 6-8 weeks,weighing 250-280 g,were anesthetized.Their hearts were excised and perfused in a Langendorff apparatus.Eighty-four isolated rat hearts with I/R injury were randomly divided into 7 groups (n =12 each):normal control group (NC group),I/R group,sevoflurane preconditioning group (S + I/R group),normal control plus wortmannin group (NC + W group),I/R plus wortmannin group (I/R + W group),sevoflurane preconditioning plus wortmannin group (S + I/R + W group),and solvent group (S + I/R + D group).In NC group,the hearts were continuously perfused with K-H solution for 180 min.In I/R group,the hearts were perfused with K-H solution for 30 min,and then subjected to ischemia for 30 min followed by 120 min of reperfusion.In S+ I/R group,the hearts were perfused with K-H solution for 10 min,and then with K-H solution saturated with 2.5％ sevoflurane for 15 min,followed by 5 min washout with K-H solution before ischemia.In NC + W group,wortmannin (PI3K inhibitor) 15 μg/kg was injected intraperitoneally at 30 min before chest opening,and the other procedures were similar to those previously described in group NC.In I/R + W group,wortmarnin 15 μg/kg was injected intraperitoneally at 30 min before chest opening,and the other procedures were similar to those previously described in group I/R.In S + I/R + W group,wortmannin 15 μg/kg was injected intraperitoneally at 30 min before chest opening,and the other procedures were similar to those previously described in S + I/R group.In S + I/R + D group,dimethyl sulfoxide 1.5 ml/kg was injected intraperitoneally at 30 min before chest opening,and the other procedures were similar to those previously described in group S + I/R.The HR,± dp/dtmax,left ventricular developed pressure (LVDP) and left ventricular end-diastolic pressure (LVEDP) were recorded at the end of equilibration and reperfusion.At the end of reperfusion,coronary effluent was collected to detect lactate dehydrogenase (LDH) activity,and myocardial specimens were obtained to calculate the percentage of myocardial infract size.The expression of autophagy marker LC3-Ⅱ and Akt,phosphor-Akt (p-Akt),mammalian target of rapamycin (mTOR),and phosphor-mTOR (p-mTOR) was determined by Western blot.Results Compared with NC group,no significant change was found in the parameters of hemodynamics in NC + W group,and HR,± dp/dtmax and LVDP were significantly decreased,LVEDP,myocardial infract size,and LDH activity were increased,LC3-Ⅱ expression was up-regulated,and the expression of p-Akt and p-mTOR was down-regulated in the other groups.Compared with group I/R,HR,± dp/dtmax,and LVDP were significantly increased,LVEDP,myocardial infract size,and LDH activity were decreased,LC3-Ⅱ expression was downregulated,and the expression of p-Akt and p-mTOR was up-regulated in S + I/R and S + I/R + D groups,and no significant change was found in each parameter in S+ I/R+ W group.Compared with S + I/R group,HR,± dp/ dtmax and LVDP were significantly decreased,LVEDP,myocardial infract size and LDH activity were increased,LC3-Ⅱ expression was up-regulated,and the expression of p-Akt and p-mTOR was down-regulated in S + I/R + W group,and no significant change was found in each parameter in S + I/R + D group.Conclusion Sevoflurane preconditioning can decrease autophagy of myocardial cells during I/R through activating PI3K/Akt signaling pathway and enhancing mTOR activity in the downstream,thus protecting isolated rat hearts against I/R injury.

Objective To investigate the clinic effect of hypocellular acute myelogenous leukemia (AML) patients with CAG regimen. Methods 29 cases with hypocellular AML were treated with CAG regimen. Results There were 14 cases achieved complete remission (CR), accounting for 48.3 %; 7 cases partial remission (PR), accounting for 24.1%. The total effective rate was 72.4 %, inducing 7 cases of NR, one case dead in early time. Conclusion CAG regimen has been proved to be effective in shortening the period of reduced peripheral blood granulocytes and decreasing the death rate related to chemotherapy, hence,a secure and effective therapy in treating hypocellular AML.