BACKGROUND:Pancreas or islet cel transplantation and stem cel transplantation bring hope to cure diabetes, but pancreas or islet transplantation appears to have a lack of donors as wel as immune rejection problems, limiting their clinical development. Therefore, stem cel transplantation therapy has become the current hotspot.
OBJECTIVE:To study the therapeutic effects of huaman telomerase reverse transcriptase (hTERT)-modified bone marrow mesenchymal stem cel s transplantation on diabetes mel itus in SD rats.
METHODS:Bone marrow mesenchymal stem cel s were transfected with PLXSN carrying hTERT. Thirty-six male SD rats were randomly divided into control group (n=6), stem cel group (n=10), hTERT transfection group (n=10), diabetes mel itus group (n=10). Except the control group, the rats were injected with stretozotocin (45 mg/kg) to make diabetes mel itus models. After modeling, rats in the stem cel group and hTERT transfection group were respectively intravenously injected with 1 mL of bone marrow mesenchymal stem cel s (1.5×1010/L) and 1 mL of hTERT-modified bone marrow mesenchymal stem cel s (1.5×1010/L).
RESULTS AND CONCLUSION:At 24 hours after modeling, the fasting blood-glucose level was significantly increased in the diabetes mel itus group, which was higher than the normal value (6.7 mmol/L). At 15 days after cel transplantation, the fasting blood-glucose levels were signficiantly decreased in the stem cel group and hTERT transfection group as compared with the diabetes mel itus group (P<0.05), but the body mass of rats was increased in these two group (P<0.05), especial y in the hTERT transfection group. At 45 days after cel transplantation, the fasting blood-glucose level and body mass in the stem cel group and hTERT transfection group were close to those in the control group (P>0.05), and moreover, the hTERT group had better outcomes than the stem cel group. Meanwhile, in the diabetes mel itus group, the fasting blood-glucose level was stil at a higher level, and the body mass decreased continously. These findings suggest that hTERT-modified bone marrow mesenchymal stem cel transplantation is effective for treatment of diabetes mel itus in rats.

BACKGROUND:Studies have reported that underin vitro experimental environment, antisense endothelin nucleic acid nanometer carrier can express target nucleic acid and produce RNA interference effect after enfolding by 12-alkylated chitosan nanoparticles, which can effectively inhibit the excessive generation of endothelin from inflammatory cytokines induced by alergen. OBJECTIVE:To investigate the effect of 12-alkylated chitosan nanoparticles enfolding antisense endothelin converting enzyme RNA expression plasmid on airway hyperresponsiveness in asthmatic mouse models. METHODS: Forty Balb/c mice were randomly divided into four groups: control, chitosan nanoparticles, normal saline and plasmid groups. Mice in the chitosan nanoparticles, normal saline and plasmid groups were subjected to sensitization by an intraperitoneal injection with ovalbumin (0, 14 days) and motivation by aerosol inhalation of ovalbumin (24, 25, 26 days) to induce asthma models. Mice in the control group were subjected to sensitization and motivation by the perfusion of normal saline. At 24hours before the first excitation, mice in the control, chitosan nanoparticles, normal saline and plasmid groups were perfused with normal salinevia airway, 12-alkylated chitosan nanoparticles, normal saline and antisense endothelin converting enzyme RNA. At 48 hours after the last excitation, the airway reactivity of mice was detected. After 28 days, bronchoalveolar lavage fluid cytology, lung histopathology, cytokines in spleen cel culture supernatant were detected. RESULTS AND CONCLUSION:Compared with the control group, the total number of plasmid cels, the percentage of eosinophils, eosinophil absolute counts, interleukin-4 levels, endothelin levels and airway hyperresponsiveness of mice in chitosan nanoparticles, normal saline and plasmid groups were increased (P < 0.05), and the lung inflammation was more severe. These indicators in the chitosan nanoparticles group were al lower than those in the normal saline and plasmid groups (P < 0.05) and the degree of inflammation was lighter than that in the saline and plasmid groups. These results demonstrate that 12-alkylated chitosan nanoparticles enfolding antisense endothelin converting enzyme RNA expression plasmid can reduce the synthetic amount of asthma endothelin and inhibit airway responsiveness.

Primary liver cancer ( PLC) is one of the most invasive malignant tumors in the clinics .The PLC within Milan criteria can be cured by liver transplantation or liver resection .However,PLC exceeding Milan criteria has been a question for us to overcome all the time .In recent years ,transcatheter arterial chemoemboliza-tion( TACE) and its combination therapy for PLC exceeding Milan criteria have made some breakthrough .A great deal of literatures have confirmed that TACE and its combined therapy for PLC exceeding Milan criteria can im -prove the survival rate obviously,it also contains many advantages,such as low cost,safe,painless,minimally in-vasive,repetitive operation and so on .The progress of TACE for PLC exceeding Milan criteria in recent years is summarized in this paper .

Parkinson disease (PD) is a common neurodegenerative disease,of which clinical symptoms give priority to movement disorders,accompanied by a series of non-motor symptoms,such as pain.Studies suggest that 40％ to 85％ of PD patients combined with pain,of more nature is related to muscleskeletal and dystonia.PD combined with pain severely reduces the quality of life of patients.However,it is still lack of uniform clinical evaluation and effective treatment.This paper makes a brief overview about the status and research progress of the evaluation and treatment of PD combined with pain.

BACKGROUND:Studies demonstrated that matrix metalloproteinase-1(MMP-1) and tissue inhibitor of metalloproteinases-1(TIMP-1) exhibit important role in corneal defect of rats with alkali burns.OBJECTIVE:To observe the promoting effect of recombinant human epidermal growth factor(rhEGF) on rats' corneas following alkali burns,in addition,to explore the effect and significance of rhEGF on the expression of MMP-1 and TIMP-1 in alkali burned rats.DESIGN,TIME AND SETTING:A randomized controlled animal experiment was performed at the Laboratory Animal Center,Liaoning Medical College from March 2008 to May 2009.MATERIALS:Sixty healthy SD rats,weighting 180-210 g,with 6-8 weeks old,half male and half female.All rats were confirmed that no anterior segment disease before the experiment with slit lamp microscope examination.METHODS:Sixty rats were randomly divided into experimental and control groups,with 30 animals in each group.After the preparation of corneal alkali burn models,cornea of rats in the experimental and control groups were given rhEGF eye drops,and physiological saline eye drops respectively,fourth a day,consecutive for 21 days.Six rats of each group were killed at days 1,4,7,14 and 21 after operation.MAIN OUTCOME MEASURES:①Changes of cornea after alkali burned was observed by slit lamp microscope.②The expressions of MMP-1 and TIMP-1 in cornea were detected with immunohistochemistry and computer image analysis system at different time points.RESULTS:The slit lamp microscope results showed that the area of corneal ulcer in the experimental group was obvious smaller than the control group at the corresponding time points.The expression of MMP-1 gradually increased and reached a peak level at 14 days after surgery,then began to decrease.TIMP-1 expressed in the early phrase,but later,its activity gradually decreased,reached the lowest level at 14 days,then began to increase,and peaked at 21 days.The level of MMP-1 in the experimental group was greatly lower than that in the control group at each time points.However,the level of TIMP-1 of the experimental group was greatly higher compared with the control group.CONCLUSION:The rhEGF eye drops can improve effectively the healing of corneal epithelial defect by adjusting the proportion of matrix metalloproteinase and its tissue inhibitor.

Bidding invitation and procurement of medical equipment is one of the most important works in hospital.It can increase the working efficiency to hold the key taches of medical equipment procurement from 4 aspects including the science of decison-making,the comfirmability of bidding invitation,the right choice of the way of bidding,etc.So some important taches of medical equipment procurement are analyzed and discussed and some points for attention are raised.[Chinese Medical Equipment Journal,2008,29(2):76-77]

Objective]To study Xin'an physician Wu Cheng asthenic diseases related theory of ideas in Bujuji, in order to more comprehensively understand asthenic diseases,to broaden the thinking, enhance the clinical curative effect. [Method]From the academic source of asthenic diseases , syndrome differentiation and treatment,prescription medication,clinical academic value, make research on Wu's thought about asthenic diseases treatment. [Result]Wu Cheng combined with their own perception and experience of clinical evidence, first put forward“external damage caused by asthenic diseases”;Aiming at the asthenic diseases of the treatment,Wu Cheng creatively put forward“support solution method”, “supplementary care method”, “treatment of spleen yin”;Wu Cheng's life was exploring the asthenic diseases,the theory of asthenic diseases had good clinical academic value,not only for the development of the cause of disease theory,but also enriching the theory of health prevention.[Conclusion]Wu Cheng Bujuji rich in content and its syndrome differentiation of asthenic diseases, with much experience, as today, treatment based on the thought of clinical asthenic diseases treatment still has important guiding significance.

Objective To investigate the significance of serum cortisol and high‐sensitivity C‐reactive protein (hs‐CRP) test in elderly patients with neurasthenia .Methods A total of 160 elderly patients with neurasthenia in our hospital from January 2012 to June 2015 were selected as the research subjects (observation group) and 160 individuals undergoing the physical examination as the control group .The differences of serum BDNF ,CORT and hs‐CRP levels were compared between the two groups .The patients were divided into the obvious anxiety group and anxiety group according to the Hamilton Anxiety Scale (HAMD) .The differences of serum BDNF ,CORT and hs‐CRP levels were compared between these two groups ;meanwhile serum BDNF ,CORT and hs‐CRP levels after treatment in the patients with ideal curative effect were detected and the detection results were statistically compared with those before treatment .Results The BDNF and CORT levels in the observation group were significantly lower than those in the control group ,while the hs‐CRP level was significantly higher than that in the control group ;the BDNF and CORT levels in the obvious anxiety group were significantly lower than those in the anxiety group ,while the hs‐CRP level was significantly higher than that in the anxiety group ;the treatment outcome also showed that the BDNF and CORT levels after treatment in the patients obtai‐ning the ideal effect were significantly increased compared with the pre‐treatment levels ;while the hs‐CRP level was significantly decreased .Conclusion BDNF ,CORT and hs‐CRP are significantly correlated with neurasthenia occurrence and its severity ,which deserves to pay attention to in clinical practice .

Objective To investigate the effects of gemcitabine,a cytotoxic drug,combined with photodynamic therapy (PDT) in treatment of human pancreatic cancer xenograft in nude mice.Methods The animal model of human pancreatic cancer was developed by suturing small pieces of SW1990 tumors into the dorsum of nude mice.Sixty animal models were randomly divided into five groups with 12 each:control group ( without any treatment ), photosensitizer group ( 2 mg/kg of Photosan, without illumination ),chemotherapy group (receiving 50 mg/kg of gemcitabine i.p. on day 0,3,6 and 9 after transplantation),photodynamic group (2 mg/kg photosan combined with laser irradiation) and combination group (50 mg/kg of gemcitabine and 2 mg/kg of photosan combined with laser irradiation). The tumor sizes were measured twice every week.All mice were sacrificed after 21 days.The tumor volume was calculated,and inhibitory effect and changes before and after treatment were analyzed.Results The tumor was grown bigger in control,photosensitizer and chemotherapy groups (all P value ＜0.05).On day 6,9,12,15,18and 21, the tumor size was significantly smaller in photodynamic group than those in control and photosensitize groups.While the tumor size on day 18 and 21 was smaller in combined group than those in photodynamic group(all P value ＜0.05). The tumor mass was (0.29 ± 0.20) g in combined group,which was lower than that in photodynamic,control,photosensitize and chemotherapy groups[(0.69±0.23) g,(1.65±0.21) g,(1.62±0.12) g,(1.37±0.19) g,respectively,P＜0.05].The inhibitory effect were 82.420%00 and 58.18% in combined group and photodynamic group,respectively(P＜0.05),while there was 1.80% and 17.00% in photosensitize and chemotherapy groups,respectively.Conclusions Photodynamic therapy has significant and short anti-tumor effect,but it can be significantly enhanced by combined with small dose of gemcitabine.

In this paper, POCT detection technology which has been carried out in community health institutions is summarized and introduced. The paper has made the research on the existing resistance and urgent problem of POCT in the community. At the same time, the paper described the prospect of the development of POCT detection technology in community health institutions in view of the future.
Humans ; Point-of-Care Systems ; Public Health