Objective To investigate the influencing factors of medication compliance of glucocorticoid for patients with interstitial lung disease (ILD). Method The outline in-depth interviews with 15 patients with ILD was done using content analysis method, and the influence factors of patients medication compliance were analyzed. Result The influence factors of medication compliance in ILD patients can be summarized as the following three themes: patients′individual factors, factors related to medical and absence of social and family support. Conclusion Nurses should master the influencing factors of hormone medication adherence in ILD patients, ready to take appropriate intervention measures, improve the medication compliance in patients with ILD hormone and promote their rehabilitation.

Objective　To investigate the influencing factors of rifampicin resistance and the mutation frequency and distribution of rpoB gene in Mycobacterium tuberculosis, and to analyze the mutation characteristics of resistance-determining region (RRDR), so as to provide reliable laboratory evidence for the epidemic trend, prevention, and treatment of rifampicin-resistant tuberculosis. Methods　A total of 312 patients with pulmonary tuberculosis who were treated in the Second People's Hospital of Fuyang from March 2019 to December 2021 were selected. After Xpert MTB/RIF and liquid drug susceptibility test, 156 patients with rifampicin-resistant pulmonary tuberculosis were selected as the rifampicin-resistant group, and 156 patients with non-drug-resistant pulmonary tuberculosis were selected as the general group. The general information and rpoB gene mutation of the two groups of patients were analyzed. Results　Among the 312 cases of pulmonary tuberculosis patients, the presence of complications, retreatment, self-medication, non-standard medication, treatment interruption, and low awareness of transmission routes were significantly higher in the rifampicin-resistant group than in the general group, and the differences were all statistically significant; logistic regression analysis showed that comorbidity and retreatment were the risk factors of rifampicin resistance. Among the 156 rifampicin-resistant specimens, 152 (97.44%) had single-gene mutations and 4 (2.56%) had double-gene mutations. The highest single-gene mutation was ProbeE, followed by ProbeD; the double-gene mutations were D+E, followed by A+B; statistically significant differences were observed for ProbeE between the initial treatment and retreatment groups (χ2=3.97, P<0.05), while others showed no statistically significant differences; among 118 multidrug resistance mutations, ProbeE accounted for the highest proportion, followed by ProbeD; there were 38 cases of single rifampicin resistance, and the proportion of mutation sites from high to low was ProbeE and ProbeD; all 4 cases of double-gene mutations were MDR-TB; there were no statistically significant differences in mutation sites between MDR-TB and RR-TB. 　Conclusions Attention should be paid to pulmonary tuberculosis patients with comorbidities and retreatment patients, and timely intervention should be given to reduce the probability of rifampicin resistance. The rpoB gene mutations in rifampicin-resistant patients are mainly single-probe mutations, with no difference in the rpoB gene mutations sites between the rifampicin-resistant patients and multidrug-resistant patients. Keywords: Mycobacterium tuberculosis; rifampin; gene; mutation

AIM protective effects of the Marine Face Pack (MFP) on hairless mice damaged by ultraviolet B(UVB) and it's relation to antioxidants were studied. MATHODS oxidative damage models of hairless mice irradiated by ultraviolet B (radiating intension; UVB 5. 15 ? 10-2J/cm2 ? 30 day) were established. The mice were randomly divided into four groups: control, UVB radiated groups (Water model, 10%MFP, 10%VitC). The ultrastructures of skin were observed by electromicroscope. The expression of Bcl - 2 protein and NOS were examined by immunohistochemical methods. The contents of MDA, the activities of GSH-Px, SOD and the T-AOC in the supernates of skin of those mice were detected by biochemical methods. RESULTS The large dilatant vacuolations and few rough endoplasmic reticu-lum in fibroblasts and epidermic cells of mice in UVB model group were observed under electromicroscope. MFP could keep the ultrastructure of epidermis and fibroblasts in the mice of MFP groups normal. MFP could enhance the expression of Bcl-2 protein and inhibit the ac-tivitiy of NOS(P

Objective To observe the effects of grape seed proanthocyanidin extract(GSPE) plus atorvastatin treatment on matrix metalloproteinase-9 ( MMP-9) in experimental atherosclerosis rabbits and to explore its mechanism. Methods Thirty male New Zealand rabbits were randomized into five groups. The normal control group were fed with standard diet for 24 weeks. And the other groups were fed with standard diet containing 1 % cholesterol for 12 weeks. In the sequential 12 weeks, the model control group was fed with standard diet. The GSPE group was fed with standard diet containing 1% grape seed proanthocyanidin(GSP). The atorvastatin group was fed with standard diet containing atorvastatin(2. 5 mg · kg~(-1) · d~(-1)). The GSPE plus atorvastatin group was fed with standard diet containing 1% GSP and atorvastatin (2. 5 mg~(-1) · kg~(-1) · d~(-1)). Blood samples were drawn from ear middle arteries of rabbits just before the experiment and at the 12th and the 24th weekend of the experiment. All the rabbits were fasted for at least eight hours before the blood was drawn. The blood samples were analyzed for the matrix metalloproteinase-9 (MMP-9). All the rabbits were sacrificed at the 24th weekend, and the expression of MMP-9 was observed in the thoracic aortic tissue using immunohistochemistry technique. Results The serum level and aorta expression of MMP-9 were increased in model group compared to control group (all P< 0. 05). The severity of atherosclerosis was less in three drug groups than that in model control group. The GSPE,atorvastatin and GSPE plus atorvastatin groups versus model group showed less atherosclerotic lession, the decreased expression of aorta MMP-9 and the decreased serum level of MMP-9 C(1. 06±0. 21), ( 1. 07 ±0.20), (0.81 + 0.16) vs. (1. 32±0. 24)ng/ml,all P<0. 05]. The effect in the GSPE and atorvastatin group was most obvious. Conclusions GSPE plus atorvastatin group has the most efficacy of anti-atherogenesis, which is associated with its reducing the expression of matrix metalloproteinase.

Objective To analyze the effect of PDCA cycle quality management model on multi-drug resistant organism (MDROs) infec-tion control in a tertiary rehabilitation hospital. Methods From March, 2013 to December, 2015, targeted surveillance of MDROs infection control was performed with PDCA cycle quality control. The MDROs detection rate, the awareness rate of MDROs prevention and control, the rate of doctor issuing isolation orders and execution rate of medical sanitation disinfection and isolation were analyzed. Results The de-tection rates of methicillin-resistant staphylococcus aureus and Carbapenem-resistant Pseudomonas aeruginosa decreased (χ2>3.922, P<0.05), while no significant difference was found in the detection rate of multidrug-resistance Acinetobacter baumanii (χ2=8.775, P=0.071). The awareness rate of MDROs prevention and control, the rate of doctor issuing isolation orders and execution rate of medical sanitation dis-infection and isolation significantly improved (χ2>399.17, P<0.001). Conclusion PDCA cycle quality management model played an impor-tant role in the prevention and control of MDROs in rehabilitation hospital.

Objective To explore the clinical value of combined detection of serum beta human chorionic gonadotropin (β-hCG),estradiol (E2),progesterone (P) and antiphospholipid antibodies (ACA) in predicting the outcomes of early threatened abortion.Methods 80 pregnant women were chosen from six to eight weeks singleton pregnancies,50 cases of threatened abortion and 30 cases of normal pregnancy.The serum levels of β-HCG,estradiol,progesterone and ACA-IgG,ACA-IgM and ACA-IgA were detected.The threatened abortion patients were given intramuscular progesterone 20 mg/day/time for 1 week,then,the pregnancy outcomes were analyzed and the serum levels of β-HCG,estradiol and progesterone were explored.Results The serum β-HCG,estradiol and progesterone of the threatened abortion group [(5 140.77 ± 2 365.47) IU/L,(680.28 ± 306.06) pg/mL,(15.59 ± 3.65) pg/L]were lower than those of the normal pregnant women [(14 520.4 ± 11 016.24) IU/L,(1 142.81 ± 670.57) pg/mL,(27.6 ± 4.78) pg/L] (t =3.224,P =0.003 ; t =2.43,P =0.022 ; t =7.72,P =0.001).According to pregnancy outcome,patients with threatened miscarriages were divided into the two groups,including 45 patients with continuing pregnancies and 5 patients with inevitable miscarriages.The serum levels of β-HCG,estradiol and progesterone in ongoing pregnancies group [(10 829.42 ± 6 926.28) IU/L,(832.9 ± 397.91) pg/mL,(25.62 ± 4.51) pg/L] were higher than those in inevitable miscarriages [(6 310.14 ± 2 593.19) IU/L,(487.14 ± 275.47) pg/mL,(13.6 ±4.84) pg/L] (P ＜ 0.05) ;The serum levels of ACA-IgG,ACA-IgM and ACA-IgA in 80 cases tested,only 2 cases who appeared in the threatened abortion group were detected with the positive of serum ACA-IgG,accounted for 4％ (2/50) (x2 =68.05,P =0.000),occurred spontaneous abortion in the end.Conclusion Combined detection of serum ACA,β-HCG,estradiol and progesterone may be helpful for prognosis of threatened abortion.However,the clinical predictive value of ACA needs further study in the early threatened abortion.

Objective To observe the effect of grape seed proanthocyanidin extract (GSPE) on vascular remodeling in ouabain-induced hypertensive rats.Methods A total of 30 male SpragueDawley rats were randomized into 3 groups:control group (received 0.9％ 1 ml normal saline by intraperitoneal injection and oral gavage in the morning),ouabain treatment group (received 2 mg/kg ouabain by intraperitoneal injection and 0.9 ％ 1 ml normal saline by oral gavage in the morning),and GSPE treatment group (received 2 mg/kg ouabain by intraperitoneal injection and 250 mg Kg 1 d-1 GSPE by oral gavage in the morning).Blood pressure was determined before and 5 weeks after the treatment.The aortas were observed 5 weeks after the treatment.The mRNA and protein levels of nuclear factor-κB (NF-κB p65) and transforming growth factor-β1 (TGF-β1) in rat aorta were detected using real-time PCR and Western blot,respectively.Morphological observations were obtained by Hemotoxylin and Eosin staining and Electron microscope.Results The systolic blood pressure was significantly lower in GSPE treatment group than in ouabain treatment group [(133.6±6.0) mm Hg vs.(146.5±7.9) mm Hg,1 mm Hg=0.133 kPa,P＜0.01].Morphological observation showed that the thickening aortic intimal and structural disorder were found in the ouabain treatment group,and aortic intimal structural integrity were normal in the other two groups.The mRNA and protein levels of NF-κB p65 and TGF-β1 were significantly lower in GSPE treatment group than in the ouabain treatment group (NF-κBp65:2.77±0.58 vs.3.14±0.64,0.73±0.20 vs.0.93±0.21,both P＜0.05; TGF-β1:5.80±0.67 vs.6.09±0.95,0.42±0.14 vs.0.69±0.16,both P＜0.05).Conclusions GSPE may inhibit endogenous ouabain,and delay the process of elevated blood pressure and vascular remodeling by inhibiting NF-κ B p65 and (or) TGF-β3 1 pathways.

Objective To evaluate the value of fat-saturated three-dimensional fast imaging with steady state procession(FS-3D-FISP)sequence in detecting hyaline cartilage defects in osteoarthritis in rabbits.Methods Osteoarthritis was induced in eighteen male rabbits by injecting papain into the left knee joints.The same volume of sterile saline solution was injected into right knee joints as controls.MRI of knee joints was done at 1,2 and 4 weeks after injection using FS-3D-FISP sequence.The the rabbits were killed and pathohistological examinations of the femoral condylars and tibial plateaus were performed.The cartilage changes were observed by MR image according to the Outerbridge method and by pathology using Collins staging.Results There was closely correlation between MR imaging and pathology in grading of cartilage injury in grade I～II and grade III～IV(P＜0.001).Conclusion FS-3D-FISP can evaluate accurately the degree of articular injury.

Objective To investigate the association between carotid-femoral pulse wave velocity (cfPWV) and composition of the ascending aorta in patients with coronary heart disease (CAD),Methods The study population comprised 60 consecutive CAD patients who underwent coronary artery bypass graft (CABG) surgery in Qilu Hospital of Shandong University.cfPWV vas measured using an automatic device (Complior,Artech,France).A quantitative study was conducted on ascending aorta specimens by histological observation (Masson staining and weigert's resorcin-fuchsin staining) and computer image analysis.Bivariate analyses were performed to study the association between composition of the ascending aortic media and cfPWV.Results cfPWV of the CAD patients was higher (14.2±2.0) m/s than that of the normal subjects.On Masson's-stained specimen slides,disorganization of smooth muscle and focal accumulations of collagen (44.1 ± 3.9)％ were visible alongthe medial aorta of the CAD patients.Weigert's-stained cross sections of the ascending aortic media in CAD patients frequently exhibited focal breakdown or discontinuous segments of elastic fibers(18.4±3.2)％,cfPWV had a positive correlation with relative contents of collagen in the ascending aorta(r=0.68,P＜0.01)and a negative correlation vith relative contents of elastin in the ascending aorta (r=-0.59,P＜0.01),but no relation with relative contents of smooth muscle(r=0.01,P＞0.05).Conclusions The reduced aortic elasticity in CAD patients can be partly ascribed to decreased elastin,increased collagen,and their disorganization.cfPWV can reflect the quantitative changes of collagen and elastin in the ascending aortic media in CAD patients.

Objective To investigate the effects of Free fatty acids (FFAs) on extracellular matrix(ECM) mRNA expression and secretion.Methods Rat glomerular mesangial cells(HBZY-1 cells) were cultured in vitro and stimulated with OA in different concentration.The expression of collagen Ⅳ(Col Ⅳ) and fibronectin (FN) and transforming growth factor-beta1(TGF-?_1) mRNA were measured by semi-quantitative RT-PCR.The levels of Col Ⅳ and FN in cultured supernatant were determined by ELISA.Results The mRNA expression of Col Ⅳ, FN and TGF-?_1 of 25、100、400 ?mol/L stimulated OA groups were 0.94?0.17、1.16?0.15、1.28?0.19 and 0.82?0.11、0.97?0.07、1.09?0.08 and 1.15? 0.07、1.24?0.06、1.36?0.05 respectively , which increased significantly compared with their control group(0.73?0.16、0.53?0.09、 0.96?0.11 P