Objective To understand the clinical distribution and drug resistance situation of112 strains of Acinetobacter Bau-mannii isolated from the patients′sputum samples in cerebral surgery ICU in order to provide the basis for the rational use of anti-bacterial drugs in clinical therapy.Methods 112 strains of Acinetobacter Baumannii were performed the retrospective analysis.The VITEK2-Compact automatic microbiological analyzer was adopted to conduct the bacterial identification and the drug susceptibility test and the K-B method was combined as the supplement control.The Whonet 5.6 software was used for conducting the statistical analysis.Results Among 112 strains of Acinetobacter baumannii,75 strains(67.0%)were multi-drug resistant,8 strains(7.1%) were pan-resistant.The antimicrobial susceptibility tests showed that the resistant rates of Acinetobacter baumannii to mezlocillin, piperacillin and nitrofurantoin reached up to 100%.Acinetobacter Baumannii had the lower resistant rate to polymyxin B (2.7%), the resistant rates to minomycin and ceperazone/sulbactam were 4.5% and 10.7% respectively.Conclusion The drug resistance phenomena of Acinetobacter Baumannii in the cerebral surgery department ICU is serious.Minomycin,polymyxin B and ceperazone/sulbactam have the better antibacterial activities to clinically isolated Acinetobacter Baumannii and may be the alternative drugs for therapy.Clinic should strengthen the monitoring and rationally use the antibacterial drugs.

Objective To investigate the impacts of Xuebijing injection on systemic inflammatory response and immune function of patients with acute exacerbation of severe chronic obstructive pulmonary disease (AECOPD). Methods A prospective randomized controlled trial (RCT) was conducted. Forty patients with severe AECOPD were divided into control group and Xuebijing group in accordance with the random number table, each group 20 cases. Both groups were treated by routine conventional basic therapy of severe AECOPD including anti-infection, phlegm-expelling formula, bronchodilators and mechanical ventilation, etc, while in Xuebijing group, intravenous drip of Xuebijing (100 mg, twice a day for 5 days) was additionally used. The changes of data of arterial blood gas analysis, blood routine examination, C-reactive protein (CRP), procalcitonin (PCT), immune function, and acute physiology and chronic health evaluation system Ⅱ (APACHE Ⅱ) score before and after therapy were observed and compared between the two groups. The length of stay in hospital, duration of mechanical ventilation, and prognosis were also compared between the two groups. Besides, according to the difference in APACHE Ⅱ score, all the patients were divided into APACHEⅡscore≥15 score group (18 cases) and APACHEⅡscore<15 score group (22 cases), and the immune function were compared between the two groups. Results ①The immune function was descended and disordered in patients with severe AECOPD. Compared with the < 15 score group, the expressions of CD45+, CD3+, CD4+, CD4+/CD8+ ratio, B-lymphocyte, natural killer cell (NK cell) in ≥ 15 score group were significantly lowered [CD45+(×106/L):663.92±100.61 vs. 1 289.92±169.38, CD3+(×106/L):342.05±108.93 vs. 882.37±172.56, CD4+(×106/L):205.96±63.97 vs. 486.24±108.64, CD4+/CD8+ratio:0.76±0.49 vs. 1.32±0.57, B-lymphocyte (×106/L):124.77±32.72 vs. 166.06±48.02, NK cells (×106/L): 186.47±39.57 vs. 243.51±44.72, all P < 0.05]. There was no statistically significant difference in the expression of CD8+ between the ≥ 15 score group and < 15 score group (P > 0.05). ② Compared with those before therapy, the pH value, oxygenation index, arterial partial pressure of carbon dioxide (PaCO2), white blood cell count (WBC), CRP, PCT were significantly improved after therapy in both control and Xuebijing groups. Compared with those in the control group, WBC, CRP, PCT were significantly lowered in Xuebijing group [WBC (×109/L): 10.29±3.83 vs. 12.69±3.42, CRP (mg/L): 9.06±4.19 vs. 15.26±4.22, PCT (ng/L): 0.18±0.21 vs. 0.42±0.24, all P < 0.05]. There were no statistically significant differences in pH value, oxygenation index and PaCO2 between two groups (all P>0.05). The degrees of improvement of CD45+, CD3+, CD4+, CD8+ and CD4+/CD8+ ratio were more remarkable in Xuebijing group after treatment than those in control group [CD45+ (×106/L): 1 079.38±153.86 vs. 1 015.63±157.11, CD3+ (×106/L): 652.05±100.05 vs. 596.81±106.85, CD4+ (×106/L): 358.92±67.53 vs. 329.99±72.61, CD8+ (×106/L): 205.73±35.19 vs. 230.41±39.74, CD4+/CD8+ratio: 2.16±0.63 vs. 1.52±0.54, all P < 0.05]. The B-lymphocyte and NK cell before treatment and after treatment showed no statistical significant differences between the two groups (all P > 0.05). Compared with the control group, the duration of mechanical ventilation (hours: 56.25±22.87 vs. 69.45±26.59) and the length of stay in hospital (days: 11.00±0.74 vs. 14.00±2.06) were shortened, and APACHE Ⅱ score was significantly lowered in Xuebijing group (8.21±2.97 vs. 12.08±3.12, P < 0.05). The numbers of multiple organ failure and dead patients in Xuebijing group were less than those of control group, but no statistical significant differences were found (both P > 0.05). Conclusion Xuebijing injection for treatment of patients with severe AECOPD can ameliorate inflammatory response, improve immune function, shorten the duration of mechanical ventilation and the length of stay in hospital, and decrease the risk of death.

Objective To observe the short-term clinical efficacy and long-term relapse-prevention effect of acupuncture therapy in treating allergic rhinitis.Method Sixty-four moderate allergic rhinitis patients were randomized into a treatment group and a control group, 32 cases in each group. The treatment group was intervened by acupuncture, while the control group was intervened by oral administration of Loratadine tablets. The two groups were treated with 10 days as a treatment course. The Uni-VAS and Multi-VAS scores were observed before the treatment, after 3 treatment courses, and 6 months after the treatment.Result After the treatment and 6 months after the treatment, the Uni-VAS and Multi-VAS scores were significantly decreased in both groups compared with the scores before the treatment (allP<0.01), and the scores in the treatment group were significantly superior to those in the control group (P<0.01,P<0.05).Conclusion Acupuncture can improve the sings and symptoms of allergic rhinitis and can well prevent the relapse in a long term.

Objective To investigation and analysis the related factors of delayed treatmen (pre-hospital and in-hospital) in pa-tients with acute myocardial infarction (AMI) .Methods 218 patients with AMI from inpatient department of cardiology and emer-gency ,and to analyze the factors that may affect patients medical timely through investigated and analyzed more than 20 specific fac-tors ,including general information ,human and social factors ,etc .Results In 218 case of AMI ,47 cases of timely treatment (21 .6% ) ,171 cases of delaying treatment (78 .4% ) in 218 patients with AMI .The results showed that education level ,disease awareness level ,economic income ,previous history of heart disease ,chest pain level ,treatment distance ,transport choices and whether call a first-aid or not had significant differences between timely treatment group and delaying treatment group (P<0 .05) . Hospital diagnosis and treatment delay may link with waiting time ,waiting for examination ,the doctor′s advice-delivery time .Con-clusion Patients with AMI treatment delay is serious ,the awareness of patients timely treatment and hospital management system should be further improved .

Objective:Through the design mode of Radiology and radiation protection information platform, reflects the importance of radiology information platform for public service, improve the construction speed of the practical application of horizontal, the implementation of training system and supporting platform for the construction of the subject librarian personnel. Methods:The shortcoming of the information platform, puts forward the design scheme of application of accelerating the construction of information platform and corresponding countermeasures. Results:The train subject librarians and the practical application for strong information platform, give full play to their respective functions, to ensure the nuclear emergency radiation well is very necessary. Conclusion: The construct of Radiological Medicine and protection information platform of scientific integrity, effectively play the subject librarian system, is an important guarantee to improve the nuclear accident emergency response mechanism.

OBJECTIVE To know the status of nosocomial infection control of epidemic measles in medical units at all levels of the epidemic area.METHODS The scene of the correlated medical units by cross-sectional study was investigated.RESULTS There were 7 children cases included 1 case of measles,4 cases with fever and 2 ordinary childrens cases in a local city general hospital.At the rural health clinic,a total of 19 cases were studied,including 3 cases with measles infection,2 cases of suspected measles cases,10 cases of children with fever and other children 4 cases.CONCLUSIONS The infected sources concentrate in grass roots medical unit relatively,The problem of isolating infected sources,protecting high risk crowd and measures for medical staff in grass roots medical unit should be improved.

OBJECTIVE To investigate the characteristics of nosocomial infection,bacterial strains and antimicrobial resistance to provide references for the clinical application of drugs.METHODS Using the combined methods of initiative monitoring and recalling system to carry on the clinical informations′statistics,the analysis and the judgment for the 912 inpatients in the rehabilitation center from Jan Oct,2007 to oct,2008 were undertaken.RESULTS The rate of nosocomial infection was 16.9%,the infection site was in mainly respiratory tract,the next was urinary tract.The pathogens were mainly Gram-negative bacteria,in whith Escherichia coli,Klebsiella pneumoniae,and Pseudomonas aeruginosa were the top three.The resistance rate of 34 strains of E.coli to ampicillin was up to 83.0% and to quinolones was 70.0%.13 strains of P.aeruginosa were sensitive to imipenem and meropenem.The resistance rate of K.pneumoniae to the common drug was raising,to piperacillin and aztreonam was 90.0%.CONCLUSIONS The antibiotics must be choosen rationally to enhance effectiveness,reduce side effect and make the resistant strains decreasing in number.

Objective:To detect the expression of gamma-aminobutyric acid(GABA)and GABA receptors in the epithelial cells of rat submandibular gland. Methods:Immunocytochemical expression of GABA and GABA receptors in submandibular gland of SD rat was investigated by EnVision techniques.Furthermore,the distribution pattern and the functional implications of GABA system were assessed.Results:Immunoreactive GABA was detected in both acinar and ductal cells in the submandibular gland of SD rat.Strong staining for GABA was localized mainly in the cytoplasm near the basement side of epithelial cells of the excretory and striated ducts.GABA-A receptor a1 subunit was positively expressed in the acinar and ductal cells,while GABA-B receptor R1 and R2 subunit was only slight expressed in the epithelial cells of the striated ducts.However,no staining for GABA-A receptor b2 subunit was found in the submandibular gland of SD rat. Conclusion:The expression of GABA and its receptors in the epithelial cells of rat submandibular gland suggests that GABA system might be involved in the secretion and/or excretion of saliva in the rat.

Argon-helium knife is a new method for tumor therapy currently.It realizes therapy through fast freezing and fast warming histiocyte.This method is simpler than surgery,radiation therapy and chemical therapy.The side-effect of it is smaller and the cost is low.But there still are some problems after freezing,and its applicable symptom is limited,too.

Objective To investigate the changes of arterial blood flow in pregnant women with severe preeclampsia(SPE) and fetuses and the effect of magnesium sulfate on it.Methods One hundred and seventy-four SPE pregnant women in our hospital from January 2013 to January 2015 were selected as the observation group and 174 normal pregnant women as the control group.The observation group was given the magnesium sulfate treatment for 2 d.The arterial blood flow changes of middle cerebral artery,uterine artery,uterine spiral artery,umbilical artery,fetal middle cerebral artery before and after treatment in the observation group were observed.And maternal blood pressure,dizziness symptoms,no load test(NST) changes were also observed.Results The RI,PI and S/D values of pregnant women and fetal middle cerebral artery before and after treatment had no statistical differences between the control group and observation group(P＞0.05).The RI,PI and S/D values of uterine artery and uterine spiral artery before treatment in the observation group were significantly higher than those in the control group(P＜0.05);the RI,PI and S/D values of uterine artery and uterine spiral artery after treatment in the observation group were significantly lower than those before treatment(P＜0.05).The umbilical artery RI,PI and S/D values before treatment in the observation group were higher than those in the control group(P＜0.05);the PI and S/D values after treatment were lower than those before treatment,but difference was statistically significant(P＞0.05).The systolic pressure,diastolic pressure and mean arterial blood pressure after treatment in the observation group were decreased compared with treatment,and the proportion of dizziness was decreased compared with before treatment,but the difference was not statistically significant(P＞0.05),while the incidence rate of NST no response was significantly lower than that before treatment(P＜0.05).Conclusion The magnesium sulfate therapy can significantly improve the blood flow situation of uterine artery and uterine spiral artery in the patients with SPE and can improve fetal intrauterine reserve.