Objective To study the efficacy and safety of lamivudine in treatment of chronic hepatitis B with pregnancy. Methods 42 women with chronic hepatitis B in front of and during pregnancy were treated by lamivudine 100 mg once daily. The efficacy of antiviral therapy,mothers or their infants complications,the risk of perinatal transmission of HBV infection were observed. Results In lamivudine-treated group, serum HBV-DNA was not detected in 85.71%(36/42). Normalization of liver function was achieved in 88. 10% (37/42). Severe hepatitis B was not oc-cured,the rates of mothers or their infants complications and HBV perinatal transmission were also decreased obviously. Compared with control group,the differences were statistically significant(P <0. 05). Conclusions Lamivudine in the treatment of chronic hepatitis B during pregnancy was safe and effective,and conduced to improve the curative effect in treatment of chronic hepatitis B, and reduced mothers or their infants complications and HBV perinatal transmission.

Aim To investigate the effects of isoliquiritigenin(ISL) on cognition dysfunction and brain energy metabolism following repeated cerebral ischemia-reperfusion in mice.Methods Mouse models of learning and memory deficits were made by repeated occlusion of bilateral common carotid arteries and reperfusion. Step-down test and Y-maze task were used to examine the learning and memory abilities of mice. The brain energy state was analyzed by reversed-phase high performance liquid chromatogra phy. Results ISL 10, 20 and 40 mg?kg -1 ig reduced the n umber of errors and extended the latency of memory in step-down test, decreased the number of cumulated stimulation and increased the number of right choices i n Y-maze task. ISL(10, 20, 40 mg?kg -1) increased the levels of ATP, ener gy charge (EC) and total adenine nucleotides(TAN) in a dose-dependent manner. Conclusion The results suggest that ISL improves cognition dysfun ction induced by repeated cerebral ischemia-reperfusion in mice, which may be r elated to the improvement of brain energy metabolism.

Objective To evaluate the efficacy of laparoscopic operations in the treatment of ectopic pregnancy.Methods A total of 148 cases of tubal pregnancy from January 2002 to January 2005 were studied.Laparoscopic operations included 106 cases of oviduct-sparing operation(79 cases of oviduct fenestration and 27 cases of lesion clearance),37 cases of salpingectomy,and 5 cases of lesion clearance.Results The diagnosis and operation were laparoscopically completed in all the 148 cases.Persistent ectopic pregnancy occurred in 2 cases.Postoperative monitoring of serum ?-hCG levels showed the shortest recovery time to normal levels after the treatment of salpingectomy(8?1 d),and the longest one after lesion clearance (15?2 d).Regular menstruation resumed at((31?)2 d) after salpingectomy and at(43?3 d) after lesion clearance.Follow-up checkups with uterotubography in 84 cases for 1~3 months(mean,2.3 months) found a patent rate of both oviducts of 82.1%(69/84).Conclusions Laparoscopic operations is a safe and effective option in the treatment of ectopic pregnancy.

Objective To explore the feasibility of laparoscopic subtotal cholecystectomy without using titanium clips.Methods Laparoscopic subtotal cholecystectomy was performed without using titanium clips on 48 patients,who had serious adhesions at the Calot's triangle.The cystic duct was ligated by silk.Results The operations were completed uneventfully in all the cases without converting to open surgery.No massive hemorrhage or biliary leakage occurred during the operation.The patients were followed up for 3 months to 3 years(mean,18 months),during which no one developed abdominal pain,fever,or jaundice.Conclusions Laparoscopic subtotal cholecystectomy without using titanium clips is a safe and simple method for patients with serious adhesions at the Calot's triangle.The rate of postoperative complications is low.

Objective To find out the defects existing in the rinsing and sterilizing process of the automatic bottle washer and to put forward an improvement measure and efficiency assessment.Methods To make the washer more effective,an automatic brush pre-washing process and a rotary spraying device were designed and used.The total bacteria amount,the total coliform group and the disinfectant (ClO2) residual level were employed as the assessment parameters.Results An external automatic pre-washing machine was installed on the bottle washer;the bottle washer was innovated so that its spray nozzle could be in directions and the rinsing and sterilizing process could be enhanced.The results showed that the improved machine ran well in treating the polluted and recycled bottles and all the assessment indexes were better than that of before improvement.Conclusion The improved automatic bottle washer is considered effective in cleanout and sterilization of bottles with a satisfactory result.

Objective To explore the causes of an outbreak of 168 cases of incisional infection in one hospital.Methods The secretory substances of incisioral wound infection from 168 cases of operation patients were collected,cultured and identified in bacterium strain.We also did drug sensitivity test,pathological examination and investigation of environment.Results Above these experinental studies demostrated that the large-scale outbreak of incisiona infection was caused by mycobacterium chelonei in operation room.Used 0.137% glutaradehyde as disinfectant immersion surgical tools ,but the concentration too low to kill the mycobacterium chelonei,which polluted the hickeyes of surgical instrument.The pollution leading to outbreak of incisional infection.Conclusions By reason of the event lesson,authors suggest that the doctors and nurses should strengthen the detection of atypical mycobacterium,so as to reduce the iatrogenic infection,reducing,or stoping the outbreak of incisinal Infection.

Objective To realize the isolation of multidrug-resistant organisms (MDROs)from ward environment, and provide evidence for clinical prevention and control of MDRO transmission.Methods Patients with MDRO in-fection in a stomatology hospital from September 2012 to July 2014 were as trial group,12 randomly selected pa-tients without MDRO infection were as control group,environmental object surface in patients’ward were taken specimens and performed culture,isolated organisms were identified and performed antimicrobial susceptibility tes-ting.Results Of 44 patients with MDRO infection,13 patients’surrounding environment were detected MDROs,the total detection rate was 29.55%(13/44).The detection rate of methicillin-resistant Staphylococcus aureus (MRSA),multidrug-resistant Acinetobacter baumannii (MDRAB),and methicillin-resistant Staphylococcus epidermidis (MRSE)were 45.45%(5/11),66.67% (2/3)and 66.67%(6/9)respectively.Detection rates of MRSA from nasal cavity and hands of MRSA-infected patients were 72.73% and 54.54% respectively,from nasal cavity and hands of patients’caregivers were 36.36%and 18.18% respectively.The difference in bacterial count between trial group and control group was not significantly dif-ferent (all P >0.05).Conclusion Isolation rate of MDROs from ward environment of MDRO-infected patients is higher than that of non-MDRO-infected patients,monitoring,cleaning and disinfection for MDRO-infected patients’surrounding environment should be strengthened,so as to prevent the spread of MDROs in hospital.

AIM: The aim of this investigation was to confirm the best operation conditions in the membrane separation of protein from the homogenate of red earthworm, Eisenia faefida. METHODS: Ultrafiltration membrane of fresh red earthworm with the cut-off of 10 000 was used as the target process, with the homogenate flux as index, the best value of operation parameters such as pressure、 temperature, surface rotation speed and feed concentration were fixed on. RESULTS: (1) The best suitable operation conditions were 0.25 MPa, 30?C , 200 r/min, pH=6.7, 33.3% (w:w) concentration and not a bit of NaCl. (2) The molecular weight distribution of protein in the homogenate of red earthworm mainly lay between 50 000～100 000. CONCLUSION: The obtained experimental results will provide pilot scale test with data support.

Objective To explore the character of the myelodysplastic syndrome(MDS) with autoimmune disorders (AID).Methods The clinical data of 60 MDS patients were reviewed.Results 5 of 60 MDS patients had AID ,of which 3 had Graves' disease.The commonest MDS was refractory anemia with ringed sideroblasts (RAS).Conclusion MDS might occur with Graves' disease.Humoral immunological abnormality might play an important role in MDS patients with autoimmune disorders and FAB types might not be associated with this.

Objective Diabetic macular edema( DME) is a common complication of diabetic patients.Laser photocoagula-tion therapy is effective in the treatment of DME despite of some patients with unsatisfactory curative effect or recurrence.This study was to explore efficacy and safety of aescin combined with laser photocoagulation in DME treatment. Methods A total of 102 pa-tients with diabetic macular edema in our hospital were randomly divided into control group(51 cases of 67 eyes) and combined treat-ment group (51 cases of 69 eyes).Aescin combined with laser photocoagulation were applied in observation group, and laser photoco-agulation was applied in control group.Observation was made on the changes of intraocular pressure, best corrected visual acuity( BV-CA), central macular thickness(CMT) and the leakage in macular area between the two groups before and after he treatment, along with detection on adverse reactions during the treatment. Results Com-pared with pre-treatment, there were no significant differences be-
tween the two groups in intraocular pressure at 1, 2, 3, 6 months after therapy(P>0.05), and compared with control group in the same time point, the intraocular pressure showed no change significantly in combined treatment group(P>0.05).Compared with con-trol group after therapy, the average value of BVCA in observation group showed significantly decreased at 2, 3, 6 months(Z=2.130, 1.932, 2.283;P=0.037, 0.028, 0.005) while the average value of CMT showed significant increase( Z=1.979, 2.315, 3.08;P=0.045, 0.031, 0.000).Compared with control group at the same time point, the number of patients who suffered from the leakage in macular area significantly decreased in combined treatment group at 2, 3, 6 months(χ2 =4.213, 5.074, 6.625; P=0.040, 0.024, 0.010) .7 patients(13.7%) in combined treatment group suffered from stomach and nausea during the treatment, without any observation of serious adverse reaction. Conclusion Conclusion Aescin combined with laser photocoagulation is effective for the rehabilitation of patients and chconsolidate effect with high safety, which is of great clinical application.