In recent years,considerable progress has been made in the treatment of multiple myeloma(MM).However,despite the current improved prognosis of this malignancy,it always ends in relapse and therefore new therapeutic approaches are urgently needed to overcome it.The chimeric antigen receptor(CAR)-T cells targeting B cell maturation antigen(BCMA),cluster of differentiation 19(CD19),cluster of differentiation 38(CD38) and kappa light chains have been evaluated,and have achieved remarkable results in clinical trials.However,even when MM is treated with CAR-T cell therapy,most patients eventually relapse,which is the greatest limitation of this therapy.This paperreviewedthe research progress,limitations and optimization of CAR-T cell immunotherapy in the treatment of MM.

Objective To establish a HPLC method to determine the antiseptics in compound dexamethasone acetate cream and evaluate the usage rationality of antimicrobial preservatives. Methods The content of ethyl hydroxybenzoate was calculated by external standard with HPLC.The effectiveness of antimicrobial preservatives was measured according to the preservative effectiveness test in ChP(2015edition) by inoculating 5 kinds of test microorganisms including Pseudomonas aeruginosa,Escherichia coli,Staphylococcusaureus,Candida albicans and Aspergillusniger.Results The linear ranges of ethyl hydroxybenzoate was 6.63-132.6 μg/mL ( r =0.999 7 ) .The average recoveries was 99.2%.The samples from different batche had different antimicrobial effectiveness due to the content of ethyl hydroxybenzoate.Conclusion The described method can be used in the determination of the preservatives of ethyl hydroxybenzoate in compound dexamethasone acetate cream.At present the use of antibacterial agent is arbitrary, it is necessary to strengthen research and supervision.

Objective To interview the work feelings of the Middle youth patients with peritoneal dialysis. Methods Using the purposive sampling method, 11 patients were interviewed, and analyzed the content of the interview. After the interview, data were transcribed and extracted the keywords. Results Eight keywords were extracted, the patients had four positive feelings:self-perceived burden relived;more social support; more glad, negative feelings relived; peritoneal dialysis could be sure. 4 negative themes were extracted:tedious;had economic pressure;lack of self-realization;could not take the family burden. Conclusions Work can improve the living conditions of middle youth patients with peritoneal dialysis. More patients should be encouraged to return to work.

Gefitinib and erlotinib are the first generation of targeted therapeutic agents approved for the treatment of advanced non-small cell lung cancer. Despite a dramatic initial response, most patients subsequently and ultimately become resistant to the drugs. Recent studies have proposed various mechanisms of such resistance, some of which related to a second mutation in epidermal growth factor receptor, aberrant activation of other tyrosine kinase receptors, bypassing signal pathways and constitutive activation of downstream signal molecules. Other researches also associated the drug resistance to internalization of epidermal growth factor receptor and changes in tumor microenvironment. Furture studies need to focus on overcoming or reversing resistance to the drugs and developing new targeted therapeutic agents.

A systematic comparative study ou the morphology and histology of the rhizomes of polygonatum including "Huangjing" and "Yuzhu" currently available on the Chinese market werepresented. The differences between this two types of herbal medicines were given.

Objective To study the distribution and drug‐resistance of pathogens isolated from vaginal secretion in the patients with vaginitis in Dalian area so as to provide an evidence for clinical diagnosis and treatment .Methods The statistical analysis was performed on the detection results of pathogens isolated from vaginal secretion in 5 308 patients with vaginitis .Results In 5 308 patients with vaginitis ,the routine detection of vaginal secretion found that 810 cases(15 .3% ) were bacterial vaginosis ,1 110 cases (20 .9% ) were candida positive and 60 cases (1 .1% ) were trichomoniasis positive ,in which 3 526 cases were performed the patho‐gen detection ,2 615 strains of pathogen were isolated ,including 1 948 strains (74 .5% ) of mycoplasma ,136 strains (5 .2% ) of Chlamydia ,17 strains (0 .7% ) of Neisseria gonorrhoeae ,276 strains(10 .6% ) of candida ,140 strains (5 .4% ) of Gram‐positive cocci and 98 strains(3 .7% ) of Gram‐negative bacilli .The susceptibility rates of Ureaplasma urealyticum to ofloxacin ,levofloxacin ,spar‐floxacin and roxithromycin were lower ,which were 7 .8% ,18 .2% ,26 .3% and 27 .2% respectively ,the susceptibility rates to clar‐ithromycin ,doxycycline and minocycline were higher ,which were 89 .4% ,99 .8% and 99 .9% respectively .The drug resistance of Mycoplasma humenis was higher .The more sensitive antibacterial drugs were only josamycin ,doxycycline and minocycline ,the sen‐sitivity rates were 75 .3% ,96 .2% and 97 .3% respectively ,which to other antibacterial drugs were less than 7 .0% .Candida albi‐cans was generally sensitive to antifungal drugs .The susceptibility rates of Staphylococcus aureus to commonly used antibacterial drugs were higher .All of 24 strains of Streptococcus agalactiae were sensitive to penicillin .No Gram‐positive coccus resistant to daptomycin ,linezolid and vancomycin was found .No Gram‐negative bacillus resistant to daptomycin ,linezolid and vancomycin was found .No Gram‐negative bacillus resistant to imipenem and meropenem was found .Conclusion The pathogens causing vaginitis are very complex .In the treatment of female vaginitis ,clinicians should rationally use antibacterial drugs according to the results of pathogens detection and drug susceptibility test .

abstract Cell metabolite analysis is of great interest to analytical chemists and physiologists, with some meta-bolites having been identified as important indicators of major diseases such as cancer. A high-throughput and sensitive method for drug metabolite analysis will largely promote the drug discovery industry. The basic barrier of metabolite analysis comes from the interference of complex components in cell biological system and low abundance of target substances. As a powerful tool in biosample analysis, microfluidic chip enhances the sensitivity and throughput by integrating multiple functional units into one chip. In this review, we discussed three critical steps of establishing functional microfluidic platform for cellular metabolism study. Cell in vitro culture model, on chip sample pretreatment, and microchip combined detectors were described in details and demonstrated by works in five years. And a brief summary was given to discuss the advantages as well as challenges of applying microchip method in cell metabolite and biosample analysis.

Objective Research and analysis on the clinical effect of rehabilitation of linguistic disturbance of child cerebral palsy patients,mainly through the treatment of acupuncture.Methods Effect comparison between the treatments of acupuncture and the only common way of 121 cerebral palsy children.Results The treatment mainly by acupuncture was obvious better than the common one.Conclusions The treatment mainly by acupuncture is one of the effective ways of linguistic disturbance of infantile cerebral palsy,and there are good prospects and special advantages for the treatment of Traditional Chinese Medical Science

Objective:The experiment was designed to apply proteomics to analyze the signal transduction molecules in T lymphocyte strain JurkatD1.1 cells with the stimulation of prolactin.Methods:JurkatD1.1 cells were cultured in the presence of recombinant human prolactin(rhPRL).Phosphated metal affinity chromography(PMAC) and immunoprecipitation(IP) assay were applied to enrich phosphoproteins from the total cell lystates which were resolved by one-dimensional electrophoresis(1 DE) or 2 DE.The different bands and different spots in the gels between control group and rhPRL-treated group were excised,digested,and analyzed by MALDI-TOF mass spectrometry.Results:Three major protein bands were observed specifically in the rhPRL-treated group and other two major protein bands were observed specifically in the control group.The bands were excised and analyzed by MALDI-TOF mass spectrometry.Protein band a in rhPRL-treated group was successfully identified as hot shock protein 90(hsp90).Nine different protein spots which were observed in the 2 DE gels between the two groups were excised and analyzed by MALDI-TOF mass spectrometry.In the rhPRL-treated group the protein spot 4 was identified as nuclear receptor co-repressor 2 variant(NcoR 2 variant);The protein spot 5 was identified as galactose-1-phosphate uridyl transferase;And the protein spot 6 was identified as zinc finger ZIM3.Conclusion:Hot shock protein 90 participates the signal transduction pathways of prolactin.Nuclear receptor co-repressor 2 variant and zinc finger ZIM3 may play a role in the process of prolactin modulating target genes' expression.

The material of this paper comes from a real consolidation case of the radiography department of a hospital, which succeeds in sharing several automatic processors through layer 3 switching.