1.Effects of transfection of ICAP-1α and its mutants on adhesion and migration of 2H-11 cells.
Jie, ZHANG ; Wangcui, LUO ; Zhengxiang, LIU ; Jingyang, LIN ; Zhongliang, CHENG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2010;30(5):569-74
This study examined the effect of integrin cytoplasmic domain-associated protein 1α (ICAP-1α) and its mutatants T38A and I138A on the adhesion, migration and tube formation of 2H-11 cells. rAAV-ICAP-1α, rAAV-T38A and rAAV-I138A were constructed. After infection, the expression of ICAP-1α and p-ERK1/2, p-c-Jun protein was measured by Western blotting. Adhesion ability was evaluated by using MTT. Cell migration was determined by using Boyden chamber method. Tube formation test was conducted on Matrigel. The results showed that in ICAP-1α, T38A and I138A groups, ICAP-1α protein expression was increased. In T38A and I138A groups, phospho-ERK1/2, phospho-c-Jun protein expressions were significantly increased as compared with the control group and the GFP group. ICAP-1α group protein expression was obviously decreased when compared with the control group and the GFP group. Cell adhesion ratio was 0.1429±0.0080 in control group, 0.1434±0.0077 in GFP group and the ratio in T38A and I138A groups increased to 0.3210±0.0082 and 0.3250±0.0079, respectively. In ICAP-1α group, the ratio was decreased to 0.1005±0.0073. In T38A and I138A groups, the number of migrating 2H-11 cells was increased to 31.45±3.20 and 33.10±5.40 against 18.51±2.80 in control group and 20.47±3.12 in GFP group. In ICAP-1α group, the number was decreased to 12.06±1.72. The number of tube-like structures was increased to 20.41±2.54 in T38A and to 22.26±3.07 in I138A groups as compared to those of control group 12.45±1.84 and GFP group 13.63±2.71. In ICAP-1α group, the number of tube-like structures was decreased to 8.32±1.24. It was suggested that rAAV-T38A and rAAV-I138A transfection can substantially increase 2H-11 cell adhesion, migration and angiogenisis, while rAAV-ICAP-1α can greatly inhibit the effect. These effects might be correlated with ERK1/2 and c-Jun protein phosphorylation.
2.Association of growth responses during growth hormone treatment in short children with small for gestational age with their serum insulin-like growth factor-1 and IGF-binding protein-3 levels
Jingyang ZHENG ; Feng LIN ; Erli QU ; Qing CHEN
Chinese Journal of Endocrinology and Metabolism 2015;31(11):974-976
The efficacy of growth hormone (GH) treatment in short children with small for gestational age (SGA) was evaluated and the association of serum insulin-like growth factor-1 (IGF) and IGF-binding protein-3 (IGFBP-3) levels with height increments was investigated.Fifty-five short children with SGA in our hospital from Sep 2009 to Sep 2013 were collected.Before GH treatment, insulin and arginine stimulation test were performed.The children were treated with recombinant GH with 0.15 U · kg-1 · d-1 for at least 12 months.After GH treatment, the height was (120.5 ± 17.63) cm, height SDS was (-1.53 ± 1.04) SDS, HtSDS was-2.04 ± 0.47, bone age was (6.19 ± 2.34) year, growth velocity was (7.24 ± 1.19) cm/year, all these parameters were significant higher than those before GH treatment.Pre-treatment serum levels of IGF-Ⅰ and IGFBP-3 were negatively correlated with post treatment growth velocity(r =-0.37 and r =-0.31;P =0.004 and P =0.021).GH treatment could effectively improve the height of short children with SGA.Children with lower pre-treatment serum levels of IGF-Ⅰ and IGFBP-3 may reach the higher growth velocity.
3.Effects of chronic intrauterine hypoxia on intimal-media thickening and strain rate of abdominal aorta of male offspring rabbits during adult age
Huitong LIN ; Guorong Lü ; Jingyang YANG ; Boyi LI ; Zhenhua WANG
Chinese Journal of Ultrasonography 2010;19(1):73-76
Objective To investigate the the effects of chronic intrauterine hypoxia on intimal-media thickening(IMT) and strain rate(SR) in abdominal aorta of male offspring rabbits during adult age.Methods Sixten New-Zealand rabbits were assigned randomly to 2 groups: chronic intrauterine hypoxia group (CIH, 12% O_2, n = 8) and normal oxygen group (NO,21%O_2, n = 8).After delivery,2 male offspring rabbits per litter were selected and breast-fed for 3 months, randomly divided into high-fat diet and normal diet groups.Finally, there were 4 groups in this experiment:chronic intrauterine hypoxia with high fat diet (CIH + HFD, n = 8) ,non-chronic intrauterine hypoxia with high fat diet (NCIH + HFD, n = 8),chronic intrauterine hypoxia with normal diet (CIH + ND, n = 8) and normal control (NC, n = 8).At sixth months of age, Serum levels of total cholesterol(TC) and triglyceride(TG) were assayed.SR in the abdominal aorta of male offspring rabbits was evaluated by ultrasonography.Then, abdominal aorta was taken out and observed by electron microscope and IMT was measured.Results CIH increased the levels of TC and TG (P < 0.01), thickened the IMT (P<0.05) and decreased the SR(P<0.05) of abdominal aorta of male offspring rabbits during adult age.There were relevant pathological changes in different groups.All these above-mentioned profiles were aggravated significantly after feeding high fat diet (P<0.05 or P<0.01).Conclusions CIH increased the IMT and decreased the SR of abdominal aorta of male offspring rabbits during adult age.
4.Effects of early minimal enteral nutrition on the immune system in newborn infants under mechanical ventilation with intratracheal intubation
Erli QU ; Qing CHEN ; Xiurui DENG ; Yudan HUANG ; Jingyang ZHENG ; Feng LIN ; Zhenlang LIN
Chinese Pediatric Emergency Medicine 2013;20(4):383-386
Objective To study the effect of minimal enteral nutrition on the immune system of newborn infants under mechanical ventilation (MV) with intratracheal intubation.Methods Eighty-one patients under MV with intratracheal intubation were divided into two groups:treatment group and control group.Forty patients in treatmet group accepted early minimal enteral nutrition,while other 41 patients in control group accepted normal feeding,then T-lymphocytes (CD3,CD4,CD8),CD4/CD8,immunoglobulins (IgG,IgA,IgM),occurane of secondary infection,time of beginning fully enteral feeding,occurance of abdominal distension were recorded.Results There were no significant differences of T-lymphocytes (CD3,CD4,CD8),CD4/CD8,immunoglobulins (IgG,IgA,IgM) changes between the two groups in which the time of patients beginning MV under intratracheal intubation was less than five days (P > 0.05) ; while comparing patients in the two groups whose MV time was equal or greater than five days,the changes of T-lymphocytes CD3[(37.3 ± 1.6)%,(42.6 ±2.8)%],CD4[(29.1 ±1.7)%,(34.6 ±2.1)%],CD4/CD8 (17.9 ±1.8,18.9 ± 1.6),immunoglobulins IgA[(55.42 ±3.98) mg/L,(129.00 ±7.76) mg/L],IgM[(130.29 ±10.92) mg/L,(317.33 ± 11.64) mg/L] in treatment group were more significant than those in control group(P <0.05) ;furthermore the differences of the secondary infection occurance,the time beginning fully enteral feeding,intravenous nutrition and hospital stays in the treatment group [20% (8/40),(8.56 ± 1.78) d,(10.56 ± 1.78) d,(12.63 ± 1.73) d] were lower significantly than those in the control group [48.78% (20/41),(10.20 ± 1.65) d,(12.15 ± 1.69) d,(14.15 ± 1.64) d] (P < 0.05).These were no significant differences on abdominal distension and gastric retention between the treatment group [20% (8/40),17.5% (7/40)]and the control group [24.39% (10/41),19.51% (8/41)] (P > 0.05).Conclusion Early minimal enteral nutrition in infants under long time MV with intratracheal intubation could improve immune function recovery,reduce the occurance rate of secondary infection,shorten the time beginning fully enteral feeding,intravenous nutrition and hospital stay.
5.rAAV-CD151 activates PI3K/Akt/eNOS pathway and promotes neovascularization in swine
Houjuan ZUO ; Sha WEN ; Zhengxiang LIU ; Tao LIU ; Xiaochun LIU ; Yu LIU ; Jingyang LIN ; Daowen WANG
Journal of Medical Postgraduates 2003;0(07):-
Objective: The signal transduction pathway of phosphatidylinositol-3-kinase(PI3K) may play important roles in promoting angiogenesis induced by growth factors.The involvement of CD151,a member of transmember-4 superfamily,remains unclear.This study aimed to investigate the effects of the recombinant adeno-associated virus mediated CD151(rAAV-CD151) gene delivery on capillary density in myocardial infarction swine and its mechanisms.Methods: We established the swine model of myocardial infarction by coronary artery ligation,and intramuscularly injected rAAV-CD151,rAAV-GFP and rAAV-antiCD151 into the ischemic myocardium.Eight weeks after coronary artery ligation,we detected the expression of CD151,and measured capillary density by immunostaining for the von Willebrand factor.Results: Compared with the control and the rAAV-GFP groups,the rAAV-CD151 group showed a higher CD151 protein expression and capillary density in ischemic myocardium after myocardial infarction(P
6.Effect of awake tracheal intubation with intubating larynegeal mask airway on stress responses of hypertensive patients
Jingyang ZENG ; Weibin ZHUANG ; Lin CHEN ; Yangyi LI ; Shunyuan LI ; Wenqin XIE
Chinese Journal of Anesthesiology 2016;36(12):1476-1479
Objective To evaluate the effect of awake tracheal intubation with intubating larynegeal mask airway (ILMA) on stress responses of hypertensive patients.Methods Sixty hypertensive patients,aged 45-64 yr,with body mass index of 20.3-27.5 kg/m2,of American Society of Anesthesiologists physical status Ⅱ or Ⅲ,undergoing elective abdominal surgery under general anesthesia,were divided into 2 groups (n=30 each) using a random number table:direct laryngoscope group (group D) and ILMA group (group I).At 3 min after topical anesthesia (T1),while epiglottis and glottis were exposed with direct laryngoscope in group D or during ILMA insertion in group I (T2),immediately after completion of intubation (T3),when the maximum change in hemodynamics after intubation appeared (at about 15 s after tracheal tubes were placed,T4),and at 5 min after completion of intubation (T5),mean arterial pressure (MAP) and heart rate (HR) were recorded,and blood samples were collected for determination of plasma epinephrine concentrations by radio-immunity method.Successful intubation at first attempt was recorded.Results Compared with the parameters at T1,the MAP,HR and plasma epinephrine concentrations were significantly increased at T2-4 in group D (P<0.01),and no significant change was found in the parameters mentioned above at the other time point in group I (P>0.05).Compared with group D,the MAP,HR and plasma epinephrine concentrations were significantly decreased at T2-4 (P<0.01),and no significant change was found in the success rate of intubation at first attempt in group I (P>0.05).Conclusion Awake tracheal intubation with ILMA does not induce strong stress responses and is helpful in avoiding the occurrence of cerebrovascular accidents,thus increasing the safty of awake tracheal intubation in hypertensive patients.
7.Clinical observation on modified adrenalectomy in treatment of pheochromocytoma
Jingyang GUO ; Wenzeng YANG ; Yanqiao ZHANG ; Xiangyang LIN ; Wei ZHANG ; Zhenyu CUI
China Journal of Endoscopy 2017;23(5):102-104
Objective To observe the clinical effect of modified laparoscopic adrenalectomy for adrenal pheochromocytoma. Methods 23 patients with adrenal pheochromocytoma were treated with modified posterior laparoscopic adrenalectomy. The changes of blood pressure, heart rate, operation time and blood loss during and after operation were observed. Results 23 patients were successfully operated, the operation time was (62.3 ± 11.7) min, the intraoperative blood loss was (34.4 ± 17.7) ml, there has no hemorrhage occurred while 3 cases were transferred to ICU for intraoperative blood pressure and heart rate fluctuation significantly, and back to general ward after 2 days, while there was no significant difference in blood pressure and heart rate preoperatively. Conclusion Modified laparoscopic adrenalectomy is safe and effective for pheochromocytoma and holds the advantages of clear anatomy, short operative time, less bleeding and less change in blood pressure and heart rate.
8.CD151 promotes proliferation and migration of PC3 cells via the formation of CD151-integrin α3/α6 complex.
Wuxiao, YANG ; Pengcheng, LI ; Jingyang, LIN ; Houjuan, ZUO ; Ping, ZUO ; Yuanlin, ZOU ; Zhengxiang, LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2012;32(3):383-8
Over-expression of CD151 was found to be associated with metastasis and poor prognosis of prostatic carcinoma. This study was designed to examine the mechanism by which CD151 promotes the proliferation and migration of prostatic cancer cells. The pAAV-CD151, pAAV-GFP and pAAV-CD151-AAA mutant plasmids were constructed and used to transiently transfect PC3 cells (a prostatic carcinoma 3 cell line) by the mediation of Fugene HD. Then, the cells were assigned to control group, pAAV-GFP group, pAAV-CD151 group, and pAAV-CD151-AAA group respectively. Cell proliferation was evaluated by using the 3-[4,5-dimet-hylthiazol-2-yl]-2,5, diphenyltetrazolium bromide (MTT) method. Cell migration assay was performed by using Boyden chambers. The formation of CD151-integrin α3/α6 complex was determined by the method of co-immunoprecipitation. The protein expression levels of CD151 and extracellular signal-regulated kinase (ERK) were measured by Western blotting. The results showed that transfection of pAAV-CD151 or pAAV-CD151-AAA mutant increased the expression of CD151 protein in PC3 cells. Co-immunoprecipitation showed that more CD151-integrin α3/α6 complex was formed in the pAAV-CD151 group than in the control group, the pAAV-GFP group and the pAAV-CD151-AAA mutant group. Furthermore, the proliferative and migrating capacity of PC3 cells was substantially increased in the pAAV-CD151 group but inhibited in the pAAV-CD151-AAA mutant group. CD151 transfection increased the expression of phospho-ERK. Taken together, it was concluded that CD151 promotes the proliferation and migration of PC3 cells through the formation of CD151-integrin complex and the activation of phosphorylated ERK.
9.Effects of CD151 on the expression of the integrin αβ1/α6β1 in the HUVECs
Yu LIU ; Zhengxiang LIU ; Houjuan ZUO ; Jingyang LIN ; Zhongliang CHENG ; Daowen WANG
Journal of Chinese Physician 2009;11(8):1009-1013
sfection could promote the expression of integrin α3β1/α6β1. The mechanism of regulating integrin α3β1/α6β1 may lie in allosterism of an extracellullar CDI51 site (QRD194-196) and C -terminal Ves. transportation target motif(YRSL245-248).
10.CD151 promotes proliferation and migration of PC3 cells via the formation of CD151-integrin α3/α6 complex.
Wuxiao YANG ; Pengcheng LI ; Jingyang LIN ; Houjuan ZUO ; Ping ZUO ; Yuanlin ZOU ; Zhengxiang LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2012;32(3):383-388
Over-expression of CD151 was found to be associated with metastasis and poor prognosis of prostatic carcinoma. This study was designed to examine the mechanism by which CD151 promotes the proliferation and migration of prostatic cancer cells. The pAAV-CD151, pAAV-GFP and pAAV-CD151-AAA mutant plasmids were constructed and used to transiently transfect PC3 cells (a prostatic carcinoma 3 cell line) by the mediation of Fugene HD. Then, the cells were assigned to control group, pAAV-GFP group, pAAV-CD151 group, and pAAV-CD151-AAA group respectively. Cell proliferation was evaluated by using the 3-[4,5-dimet-hylthiazol-2-yl]-2,5, diphenyltetrazolium bromide (MTT) method. Cell migration assay was performed by using Boyden chambers. The formation of CD151-integrin α3/α6 complex was determined by the method of co-immunoprecipitation. The protein expression levels of CD151 and extracellular signal-regulated kinase (ERK) were measured by Western blotting. The results showed that transfection of pAAV-CD151 or pAAV-CD151-AAA mutant increased the expression of CD151 protein in PC3 cells. Co-immunoprecipitation showed that more CD151-integrin α3/α6 complex was formed in the pAAV-CD151 group than in the control group, the pAAV-GFP group and the pAAV-CD151-AAA mutant group. Furthermore, the proliferative and migrating capacity of PC3 cells was substantially increased in the pAAV-CD151 group but inhibited in the pAAV-CD151-AAA mutant group. CD151 transfection increased the expression of phospho-ERK. Taken together, it was concluded that CD151 promotes the proliferation and migration of PC3 cells through the formation of CD151-integrin complex and the activation of phosphorylated ERK.
Cell Line, Tumor
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Cell Movement
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Cell Proliferation
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Humans
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Integrin alpha3
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metabolism
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Integrin alpha6
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metabolism
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Male
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Prostatic Neoplasms
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metabolism
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pathology
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Tetraspanin 24
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metabolism