Objective To study the correlation of blood gas analysis and diabetes mellitus in chronic obstructive pulmonary disease(COPD)patients and its clinical significance.Methods One hundred and twenty-five patients with stable phase COPD patients including 69 cages with COPD combined with diabetes mellitus(experimental group),56 patients with COPD alone(control group).The blood gas analysis and pulmonary function were detected.Results The simple pulmonary function forced vital capacity(FVC),vital capacity(VC),forced expired volume in one second(FEV1),peak expiratory flow (PEF)(25%-75%)and the diffusion function parameters diffusion capacity for carbon monoxide of the lung (DLCO),DLCO/PRED in control group were significantly higher than those in experimental group(P<0.05).Arterial partial pressure of oxygen(PaO2)was lower in experimental group than that in control group[(61.23±8.34)mm Hg(1 mm Hg=0.133 kPa)vs.(72.34±9.32)mm Hg,P<0.05],while there was no significant difference in partial pressure of carbon dioxide in artery(PaCO2)between two groups[(45.34±6.43)mm Hg vs.(44.89±4.98)mm Hg,P>0.05].Conclusion COPD combined with diabetes mellitus may lead to some damage in lung function and diffusion function,it should be noted in the clinieal treatment of reasonable adjustments in treatment.

Objective To investigate the expression and significance of hypoxia-inducible factor 1 alpha (HIF- 1α), vascular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2) in tissues of condy- loma acuminatum (CA) in pregnant women. Methods Tissue specimens were obtained from the lesions of 30 pregnant women with CA and 30 non-pregnant women with CA, and from the vulva of 15 normal preg- nant women. By immunohistochemical staining, the expressions of HIF -1α, VEGF and Ang-2 were detected in these specimens. Results The expression rates of HIF -1α,VEGF and Ang-2 were 86.67%, 93.33% and 83.33% in pregnant women with CA, respectively, 63.33%, 66.67% and 53.33% in non-pregnant women with CA, respectively, and 0, 6.67% and 0 in normal women, respectively. Enhanced expressions of HIF -1α,VEGF and Ang-2 were observed in pregnant women with CA compared with the latter two groups (P < 0.05). In pregnant women with CA, a significant correlation was noted between the expression of HIF -1α and VEGF (r = 0.412, P < 0.01) and between the expression of Ang-2 and VEGF (r = 0.460, P < 0.01). Further more, the expression of HIF -1α, VEGF and Ang-2 positively correlated with each other in non-preg- nant women with CA. Conclusion In tissues of CA in pregnant women, HIF-1α,Ang-2 and VEGF are over expressed, which may be related to angiogenesis.

Post-traumatic brain syndrome is a common disease in neurosurgery department, referring to a variety of neurological symptoms after brain traumas. Currently, research shows that this disease involves the fields of neurologic anatomy, pathology, trauma mechanics, neurobiochemistry, cerebral hemodynamics, etc, and the treatment majorly includes medication, hyperbaric oxygen, and psychotherapy, but they don't work so well. In primary hospitals, the convenient, low-cost and safe acupuncture-moxibustion therapy should be paid more attention. By retrieving literatures during the latest 10 years, this article has concluded that the acupuncture-moxibustion treatments for this disease covered acupuncture, auricular acupoint therapy, bloodletting therapy, and moxibustion, and the action mechanisms were mostly the improvement of cerebral circulation and cerebral metabolism, and the change of neurotransmitters and cytokines. The improvement of cerebral circulation and increase of cerebral blood flow can be taken as the future direction in the research on acupuncture-moxibustion for this disease. However, in the treatment of post-traumatic brain syndrome with acupuncture-moxibustion, it still requires the standardization of acupuncture and the objectification of evaluation indexes.

At present,there are many problems in the cultivation for medical students including lack of humanistic education,insufficient healthy personality shaping and innovation education.We made explorations in aspects of updating education concept,enhancing faculty construction,emphasizing basic course teaching and promoting students' abilities so as to cultivate the comprehensive quality of students majored in medical imaging (ultrasonic medicine).

Objective To investigate the extraction technique for atractylenolide Ⅰ in Atractylodes macrocephala by supercritical CO_2 fluid extraction and develop a method used for determining the content of atractylenolide Ⅰ in the extract by HPLC. Methods The effects of seven facters, such as the extracting pressure, resolving pressure etc, to the extraction rate of atractylenolide Ⅰ in A. macrocephala by supercritical CO_2 extraction were investigated. RP-HPLC was used to determine the content of atractylenolide Ⅰ in extraction of A. macrocephala. The separation was performed on Hypersil ODS2 column with methanol-water (70∶30) as mobile phase. The flow rate was 1.0 mL/min and the wavelength of UV detector was 220 nm. Results The optimal extracting conditions: taking 10% alcohol as entraiter, the particle size of medicinal substances was 60 screen meshes, extracting pressure 25 MPa, resolving pressure 5 MPa, extracting temperature 40 ℃, resolving temperature 30 ℃, and the extracting time 4 h. Conclusion Supercritical extraction is time-shorter and efficient in extracting atractylenolide Ⅰ from A. macrocephala. It is suitable to both trial and industrialized production. The method established to determine the content of atractylenolide Ⅰ of A. macrocephala by supercritical extraction is simple, sensitive, and reliable.

Adult ; Calcinosis ; metabolism ; pathology ; Diagnosis, Differential ; Fibroma ; metabolism ; pathology ; Gastrointestinal Stromal Tumors ; pathology ; Humans ; Male ; Stomach Neoplasms ; metabolism ; pathology ; Vimentin ; metabolism

Objective To find out demography characteristic and risk behaviors of occupation groups and to evaluate the effect of health education. Methods A baseline survey was conducted among occupation groups in the western part of Liaoning province by stratified random cluster sampling. Health education started to intervene in occupation groups in Linghai city of Liaoning province. An investigation of brucellosis related behaviors was carried out before and after a health education among occupation groups. Other occupational groups were as the control group. The research on high-risk behaviors of occupation groups was in the following eight aspects: protection taken when contact with abortion livestock, timely slaughter and burying deeply of sick animals, not selling sick animals do not eat dead meat, nor drink raw milk, do not eat raw meat, and immunity of livestock. Results Before the intervention, a total of 916 objects, of which intervention group 499 and control group 417 were investigated. The high-risk behaviors of brucellosis were not significantly different between the two groups[70.9% (354/499), 64.3%(321/499 ), 53.8% (268/499), 92.2% (460/499), 82.2% (410/499 ), 87.5% (437/499), 93.6% (467/499), 56.2%(280/499) in intervention group, 68.8% (287/417), 59.2% (247/417 ), 54.7% (228/417), 89.2% (372/417 ), 85.6%(360/417) ,885%(369/417),94.0%(392/417),55.9%(233/417) in control group, all P ＞ 0.05]. After the intervention,a total of 908 survey objects, of which intervention group 499 and control group 409, were investigated. The above eight aspects of high-risk behaviors in the intervention group were 85.4% (426/499),79.0% (394/499),69.9%(349/499),96.4%(481/499),943%(471/499),94.9%(474/499),94.0%(469/499), 99.4%(496/499), respectively; in the control 66.8% (273/409),57.3% (234/409),50.1% (205/409),85.3% (349/409),88.8% (363/409),89.5%(366/409) ,90.1% (369/409), and 57.2% (234/409), respectively. Between the two groups, the difference was statistically significant (x2 = 43.991,49.833,37.111,35.032,9.537,9.826,4.485,253.808, all P ＜ 0.05 ). The above seven aspects of high-risk behaviors except do not eat raw dairy at the beginning of intervention were compared with those of after the intervention, the differences were statistically significant (x2 = 30.426,26.284,27.854,7.364,36.027,17.238,9270.286, all P ＜ 0.05). Conclusions Health education intervention can significantly improve the behavior change rate of occupational groups at high risk of brucellosis, and the education intervention is worthwhile to communicate and promote.

Objective To observe the CT features of mycoplasma pneumoniae pneumonia. Methods CT features of 40 adult patients with serologically proven mycoplasma pneumoniae pneumonia were retrospectively reviewed. Results The most common CT findings of mycoplasma pneumoniae pneumonia were ground-glass opacity (34/40, 85.00%) and consolidation (28/40, 70.00%). The other findings included nodules (18/40, 45.00%), thickening of the bronchovascular bundles (6/40, 15.00%), interlobular septal thickening (6/40, 15.00%), mediastinal lymphadenopathy (6/40, 15.00%) and pleural effusion (4/40, 10.00%). Most of the ground-glass opacity and consolidation showed tendency of lobular distribution and involving the lower lobes. In some patients, the absorption of the lesions was slower than the relief of symptoms. Conclusion CT findings of mycoplasma pneumoniae pneumonia have some features, and can provide help for the diagnosis of mycoplasma pneumoniae pneumonia.

Objective To develop a sensitive HPLC method for the determination of malachite green in fresh water. Methods The HPLC column of ZORBAX SB-C18 (250 mm?4.6 mm, 5 ?m) was employed and acetonitrile and acetate buffer (0.1 mol/L, pH=4.5, V∶V=80∶20) were taken as the mobile phase. The samples were tested at wave length of 588 nm after post-column oxidation with PbO2 flowed at 1.5 ml/min. Results The average recovery rate were 98.5% and 97.8% respectively at the water concentration of 2.0 ?g/L and 10.0 ?g/L,the relative standard deviations (RSD) were 11.2% and 8.0%,the least detection limit was 0.02 ?g/L. Conclusion The method is stable,sensitive and suitable for the detection of malachite green in water.

Objective To understand the clinical features of peripheral cholangiocarcinoma (PC) developed against the background of hepatolithiasis. Methods Between 1995 and 2002, a total of 11 PC patients with definite history of hepatolithiasis were treated surgically in our hospital. The diagnosis, treatment and prognosis of this clinical entity were retrospectively analyzed. Results Peripheral cholangiocarcinoma occurred in 5 1% of patients with hepatolithiasis in this group and the tumor was located in the left lobe in 7 (64 7%) cases. All patients underwent laparotomy, with diagnosis established preoperatively in 3 (27 2%) cases and intraoperative diagnosis was missed in 2 (18 1%) cases. Radical resections were performed in only 3 (27 2%) cases. The overall operative morbidity and mortality were 72 7% and 9 1% respectively . Infection was the main postoperative complication. The postoperative 1 and 2 year survival rate was 33 3% and 11 1% respectively. Conclusions Cholangiocarcinoma may develope in patients with a history of hepatolithiasis. The prognosis of PC is still poor because of the difficulty to achieve early diagnosis and radical resection.