Virus hepatit is a severe infectious disease which threaten people's health.There is no specific and effective methods to treat it untill now.In recent years,gene therapy,anti-hepatitis virus,has made great progress in vitro and in animal.Gene therapy is going to be a important measure for treating virus hepatitis.However,ethical problems should be solved before appling it in clinic.

The performance and clinical application of the skull repair materials differ depending on their variety. Organic glass has ever been widely applied, but its biocompatibility is poor with the high infection rate of subcutaneous effusion; Bone cement is good at histocompatibility but bad at absorption, thus can be only used for parts of skull repair; Medical silica gel polyester grid is cheap and effective, however, local infection and material's expose occur, leading to unsatisfactory appearance; Titanium and Titanium-alloy, as one kind of high polymer materials, have achieved widely application on the clinical practice for repairing skull defects due to their good biocompatibility and physicochemical property, but many shortages are also observed. With the development of the society, patients require more and more, some insufficient materials for the repair have been abandoned, and the materials for repairing skull defects should be selected according to the individual condition. In this paper, various materials of the skull repair were compared concerning the property and clinical application, to search an optimal material for the skull repair, with the good biocompatibility and good clinical efficiency.

[Objective] To investigate the relevant factors of neonatal hemolysis, especial y the association between maternal health and neonatal hemolysis. [Methods]O and Rh positive pregnant women in late pregnancy in 497 cases, make physical identification, and track whether the newborn hemolysis. [Results] There were significant differences in different physical pregnant women in the postpartum hemolysis newborn during pregnancy, the partial repletion heat, phlegm dampness postpartum hemolytic disease of the newborn was a high incidence of pregnancy, the lowest incidence rate and quality of postpartum hemolytic disease of the newborn. [Conclusion]The constitution(partial phlegm, partial heat) pregnant women for health care, to achieve peace and reduce neonatal ABO hemolytic was significant.

Lung cancer is currently one of the most common malignant tumors in the world, with an increasing trend of morbidity and mortality.The major causes of lung cancer are inhaled carcinogens, such as tobacco and environmental pollution, but a growing number of researches suggest that the incidence of lung cancer is closely related to genetic factors.In the past few years, great progress has been made in research on single nucleotide polymorphism of susceptibility gene in lung cancer, and the most studies that have aimed to ultimately provide scientific basis for preventing and controlling the lung cancer have focused on screening of lung cancer etiology and developing gene therapy.In this review, the new findings of genetic susceptibility in lung cancer have been summarized.

Objective To explore the role of LGR5 positive intestinal stem cells in repairing damage of intestinal mucosa resulting from endotoxemia and to determine whether the damaged intestinal mucosa can be repaired by regulating the proliferation and differentiation of intestinal stem cells.Methods Wistar rats were randomly divided into control group,lipopolysaccharide(LPS) group,and glucagon-like peptides 2(GLP-2) group.LPS was injected interaperitoneally into rats of the LPS group and GLP-2 group at a dose of 5 mg/kg(1 ml/kg) ; saline(1 ml/kg) was injected into the rats of control group.GLP-2 250 μg/kg(1 ml/kg) was rnjected into the rats of GLP-2 group 1 hour after the LPS injection.The terminal ilea were collected from 8 rats in each group at 6 h,24 h,and 72 h post-LPS injection.Structrral changes in the intestinal epthelium of every group were observed under the light microscope and electron microscope.The expression of LGR5 in intestinal stem cell was detected by immunohistochemical method and RT-PCR.Results In general,intestinal edema and hyperemia was observed in both LPS and GLP-2 groups at 6 h.Fracture and lodging of villi,infiltration of inflammatory cells,and visible exudate within the cavity were observed under light microscopy.The inflammatory injury was less severe in the GLP-2 group than in the LPS group at 6 h.The injury of intestina mucosa gradually repaired between 24 h and 72 h after injection of LPS in both LPS group and GLP-2 group.Importantly,samples taken from GLP-2 group and LPS group at the same time point indicated that the GLP-2 group recovered significantly better than the LPS group.And expressions of LGR5 mRNA in GLP-2 group at 6h,24h,72 h(0.13 ±0.05,0.16±0.05,0.16±0.04) were significantly higher than those in LPS group respectively (0.52 ±0.09,0.73 ±0.14,0.48 ±0.24),as shown by RT-PCR,and than that in control group(0.12 ±0.03) (P ＜ 0.05).Conclusion Exogenous GLP-2 may facilitate intestinal stem cell proliferation,thereby promoting the recovery of intestinal mucosa damaged by endotoxemia.GLP-2 appears to promote stem cell proliferation,differentiation,and gradual migration of stem cells from the intestinal crypts to the damaged mucosa.

Surgery is still the basic treatment of esophageal carcinoma. Quality of life, a new evaluation criterion for the health and disease, reflects the surgical outcome of esophageal cancer comprehensively. The findings of clinical research demonstrate that in the surgical treatment of esophageal carcinoma, quality of life could provide us with new selections such as surgical method, lymph node dissection and digestive tract reconstruction. Further application of quality of life scale in esophageal carcinoma surgical treatment, will greatly enhance the surgical outcome and improve patients'quality of life.

Objective To investigate the attitude of clinical nurses to the practice of holistic nursing and provide basis for establishing harmonious relationships between patients and nurses.Methods primary nurses were randomly selected and underwent investigations on quality check of nursing work quarterly by serf-designed inventory.Results 24 nurses considered it necessary to carry out holistic nursing but 70 of them were reluctant to undertake it.They thought it boring because of the high requirement of holistic nursing and much literal document in practice.Conclusion Nursing services calls out seeking truth and dealing with concrete matters at present.The holistic nursing is not equipped with professional title since serious shortage of nursing staff.The emaphasis should be focused on the health education according to different situations in hospitals.Besides we should practice holistic nursing step by step instead of by the modality.

Objective To explore the relationship of pregnancy-associated plasma protein-A (PAPPA),osteoprotegerin (OPG) and endometriosis,so as to apply the new theoretical basis for the etiology of endometriosis.Method PAPP-A and OPG in peritoneal fluid were measured in 25 women with endometriosis(study group) and 25 women with hysteromyoma ablation (control group).Results The levels of PAPP-A and OPG were both higher in peritoneal fluid in study group [(72.3±57.4)U/L and (3.28±2.63) μ g/L]than that in control group [(42.3± 19.7) U/L and (1.73 ±0.45) μ g/L] (P＜0.05).Furthermore,significant correlation was found between the stage of endometriosis and the levels of PAPP-A and OPG in study group (P＜0.05).The concentration of PAPP-A in peritoneal fluid were significantly higher in the secretory phase than the proliferative phase of the menstrual cycle in two groups(P＜0.05) ,and OPG in peritoneal fluid without significant difference(P＞ 0.05).Conclusion PAPP-A and OPG maybe play an important role in the development of endometriosis.

Objective To evaluate the effect of implantation of chitosan membrane with triamcinolone(TA) into perichoroidal space combined with intraocular vancomycin injection on the protection of retina in rabbit endophthalmitis.Methods The drug membrane was made of gelatin-chitosan.The right eyes of 30 normal pigment rabbits were inoculated intravitreally with 0.1 ml mixture of 105 CFU standard strain Staphylococcus aureus(ATCC 25923).Twenty-four hours later,rabbits were equally randomized to intravitreal vancomycin group(Group A),intravitreal vancomycin and TA eye dropping(Group B),and intravitreal vancomycin combined with TA membrane implantation(Group C).Clinical observation of ocular fundus was done every day with indirect ophthalmofundoscope.B ultrasound examination was carried out in 24 h after inoculation and on the 14th day after therapy.Electroretinogram(ERG) was done before therapy and the 14th day after therapy.Histopathologic grading and electron microscopy were carried out at the 14th day.Results After implanting the drug membrane into perichoroidal space,the inflammation in group C was markedly less than the other 2 groups,and its inflammation score was significantly lowest(P

AIM: To investigate the effect of urokinase plasminogen activator (uPA) on metastasis inhibition of gastric cancer SGC7901cells induced by parthenolide. METHODS: The changes of SGC7901 cells reproductive activity were analyzed by MTT. Light microscope was used to observe the cell morphological changes. The effects of parthenolide on migration and invasion capacity of SGC7901 cells were determined by using matrigel and transwell system. The expression of uPA was detected by Western blot and RT-PCR assays. RESULTS: Parthenolide inhibited the proliferation of SGC7901 cells in a time-and concentration -dependent manner ranging from 100 to 200 ?mol/L. Light microscopy showed the suppressing effect on growth of SGC7901 cells. After exposed to parthenolide, the migration and invasion capacity of SGC7901 cells were significantly decreased, the number of cells gradually decreased through the basement membrance. The Western blot assay showed that the expression of uPA protein declined gradually after exposed to parthenolide for various period of time. CONCLUSION: Parthenolide can inhibit the growth and metastasis activity of SGC7901 cells, and uPA played an important role in the latter process.