Objective To confirm the expression and localization of COX-2 gene in mature rat testis,as COX-2 not only plays a central role in inflammation by up-regulating the synthesis of prostaglandins,but also mediates critical reproductive functions by other mechanisms. Methods In the mature Wistar rats,COX-2 expression and its localization were examined by using immunohistochemistry,RT-PCR and Western-blot. Results The results of RT-PCR and Western-blot showed that COX-2 mRNA and its protein exist in the testis. Expression of COX-2 was localized in spermatogenic cells on immunohistochemical study.No COX-2 expression in sertoli cells and leydig cells was found. Conclusions The present study confirmed the expression of COX-2 in rat testis and its localization in spermatogenic cells.These suggest that COX-2 may play a role in spermatogenesis.

Objective To investigate the effect of Bushenzhuanggutang combined with salmon calcitonin see calcimar in the treatment of renal osteodystrophy,and explore the mechanism of treatment.Methods 48 patients with renal osteodystrophy were divided into two groups:combined treatment group and control group.The patients were treated with Bushenzhuanggutang and salmon calcitonin see calcimar in combined treatment group.The patients were treated with salmon calcitonin see calcimar in the other group.Then the levels of serum calcium、phosphorus,PTH and BUN、Scr were detected before and after the treatment.Results The level of serum calcium increased significantly in two groups,while the level of serum phosphorus and PTH decreased(P

This paper introduces some information of the electronic control system for LGJ-80 freeze dryer, including its system structure, hardware system and software system. The automatic control during overall course, automatic adjustment for degree of vacuum and antijamming are realized in this paper.

Objective To improve the freeze-dryer shelf temperature control effect.Methods The fuzzy-PID control algorithm that's adjusting the PID parameters in real-time in the control process,was adopted to control the shelf temperature,expecting to adapt the changing of process parameters of shelf.Results In the process of freeze-drying temperature control shelves,the shelf temperature static performance and dynamic performance were improved,and the effect of shelf temperature control was archived satisfyingly.Conclusion In the freeze-drying process,the process parameters of shelf are changed along with the drying time and the working environment.The conventional PID control is difficult to obtain satisfactory results.The fuzzy-PID controller,which can adjust PID parameters on real-time in the controlling process,be used in the shelf temperature control,and the result is satisfied.

Objective To explore influence of valsartan on MAU of patients with DN at early stage.Methods 163 patients with DN at early stage were randomly divided into experimental group and control group.Two groups were given routine therapy, and experimental group were added the therapy of valsartan.Period of treatment was 24 weeks, and compared the UAER, Cr, BUN of two groups.Results After treatment of 24 weeks, UAER, Cr, BUN of experimental groups was (142.4 ± 15.6) μg/min, ( 68.7 ±9.4) μmol/L, (3.5 ± 1.2 ) mmol/L, compared with (195.8±23.7)(μg/min,(93.8 ± 13.6) μmol/L, (6.3 ±1.5) mmol/L before treatment, had significant difference (t = 13.675,11.287,2.469,all P<0.05).Compared with( 199.6 ±24.7)μg/min,(87.7 ±11.3)μmol/L、(6.2 ± 1.3)mmol/L of control group after treatment, declined more significantly (t = 13.246,10.312,2.518,all P<0.05).There was no serious complications occurred in two groups.Conclusion Valsartan used in patients with DN at early stage,can significantly improve the function of kidney and enhance clinical effect,decline the side reaction.

OBJECTIVE:To optimize the extraction craft of gentiopicrin from gentian.METHODS:The extraction craft was optimized by orthogonal test with gentiopicrin as an index,and the content of gentiopicroside was determined by TLC scanning method.RESTLUTS:The quantity of menstruum and the extraction times of gentiopicrin had significant influence in the gentiopicrin extraction(P

Objective To develop a high-speed data acquisition system with sampling frequency of 2 GHz to meet the requirement of accurate measurement of flight time.Methods The ALTERA STRATIX Ⅲ series FPGA was used as the main control chip, matching with high-performance ADC chip with sampling frequency up to 2 GHz and sampling accuracy up to 12 bit, while extending 512 MB DDR3 SDRAM for data cache and exchanging data with the host computer through Gigabit Ethernet.In addition, a variety of PCB wiring methods were used to effectively ensure the signal integrity.Results The 250 MHz sine signal was input into the data acquisition board with 2 GHz sampling frequency,by analyzing and calculating the sampling data,acquired the following performance index of SNR=44.6639 and ENOB=7.1.Conclusion This high-speed data acquisition system based on ADC technology is a fast and reliable path and method for the measurement and analysis of biological mass spectrometry data.

Objective To investigate the real type of the first earlier onset spinocerebellar ataxia family in China. Methods Two family members were subjected to autopsy, whose genetypings were confirmed by polymerase chain reaction (PCR) and direct sequencing technique. Golgi staining, immunohistochemistry and electron microscopy methods were used to detect the neurodegeneration in central nervous system of 2 patients. Results The light microscopic and electron microscopic showed synaptic degeneration of Purkinje cell in the cerebellar cortex, which was accompanied by deterioration of Purkinje cell, and both inferior olivary complex, dentate nucleus and anterior central gyrus. Conclusions There is severer neurodegeneration in the central nervous system of earlier onset spiuocerebellar ataxia 6 patient, especially in cerebellar cortex, inferior olivary complex and dentate nucleus, and the neurodegeneration may depend on disease duration.

Objective To investigate the level of thyroid hormone in Xinjiang Uygur and Han old people with mild cognitive dysfunction (MCI),and to provide basis for early intervention of MCI.Methods Case control study was carried out among 5398 residents who was above 60 years old in Xinjiang,Han and Uyghur,from July 2008 to October 2010.According to the diagnosis of MCI by American Psychiatric Society and DSM-Ⅳ,314 patients were diagnosed with MCI matching 299 healthy people as control group.Serum T3 (triiodothyronine),T4 (thyroxine) and TSH (thyroid stimulating hormone) level was determined and compared between control group and MCI group with different nationalities and genders SPSS 17.0 statistical software was used to depict median and quartile of the data,two groups of distribution between the location of the median use Wilcoxon compares the rank of testing and analysis.Results (1) the level of serum T3 in MCI group was lower than that of the control group (1.26 μg/L vs.1.28 μg/L,Z=2.41,P＜0.05),serum TSH level of MCI group was 5.37 mIU/L higher than that of the control group (Z =4.13,P ＜ 0.05).(2) Male serum TSH level of MCI was 5.74 mIU/L higher than that of the control group (Z =5.09,P ＜ 0.05).Women serum T3 level of MCI was 1.29 μg/L lower than that of the control group 1.53 μg/L (Z =2.62,P ＜ 0.05).(3) Serum TSH level of MCI in Han nationality was 6.79 mIU/L higher than that of the control group (Z =4.95,P ＜ 0.05).Serum T3 level of MCI in Uygur nationality was 1.29 μg/L lower than those of the control group 1.39 μg/L (Z =2.58,P ＜0.05),and T4 level was 82.37 μg/L lower than that of the control group 92.71 μg/L (Z =4.28,P ＜ 0.05).(4) Uygur's T4 level was 82.3 μg/L,higher than the 42.12 μg/L in Han nationality (Z =9.83,P ＜ 0.05),level of TSH in Uygur with MCI was 6.79 lower than that of the Han nationality (Z =4.28,P ＜ 0.05).(5) The unconditional Logistic regression analysis showed hypertension,sugar metabolism disorders,hyperlipidemia,low level of T3 were independent risk factors for MCI (OR values were 2.014,2.466,2.096,0.470,respectively,95％ CI were 1.361-2.981,1.649-3.687,1.396-3.149,0.334-0.663,respectively,P ＜ 0.05).Conclusions (1) Thyroid hormone level in old people related to the occurrence of MCI ; (2)Aside recognized independent risk factors for MCI as hypertension,sugar metabolism disorders,hyperlipidemia,low T3 level may be one of the MCI independent risk factors.

B→O blood conversion has very important meanings in the blood transfusion during the period of peace and war.In the leader of Professor Zhang Yangpei,from Academy of Military Medical Sciences,B→O blood conversion has been achieved by using gene engineering.They obtained the great breakthrough in the domain of blood type conversion.In order to realize standardization and automatization,it is necessary to develop a device applying to B→O blood conversion.This device automatically accomplishes the process of B→O blood conversion,shortens the time of B→O blood conversion to quarter than the time by manual operation,and ensures the standardization and obturation.In the condition of one-off pipeline,this device meets the clinical demands for blood,and lays a foundation for clinical expansion of B→O blood conversion.This paper presented the control system of B→O blood conversion device,including design of software and hardware.This device provides a safe and efficient tool for B→O blood conversion.