Asthma ; drug therapy ; Autoimmune Diseases ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Graft Rejection ; drug therapy ; Humans ; Immunosuppressive Agents ; therapeutic use ; Male ; Phytotherapy

BACKGROUND:Pancreatic islet transplantationviaportal vein system leads to the apoptsis of a number of islet cels due to local hypoxia,therebyaffecting transplant outcomes. OBJECTIVE:To explore theeffect ofpre-micrvascularization network of tissue-engineered constructs on the survival of transplanted islets and the feasibility of xenogenic islet transplantation. METHODS:A 5-mm-long cylindrical silicone tube filed with Matrigel TM matrix surrounding the superficial epigastric vessel was placed in the groin ofdiabetic mice. After the syngeneic islets with 300 islet equivalents (IEQ) were transplanted into the silicone chamber on days 0, 14 and 28 post-chamber implantation, respectively, the recovery time of blood glucose was observed. The islets with the quantity of 100 IEQ, 200 IEQ and 300 IEQ, respectively, were transplanted on day 28 post-implantation and then the blood glucoselevelwas determined. Moreover, the survival of human pancreatic islets with 1 000 IEQ transplanted into the pre-vascularizated chamber or under the renal capsule of diabetic mice, folowed by the treatment of anti-CD45RB and/or anti-CD40L (MR-1) was analyzed. RESULTS AND CONCLUSION:An abundant micro-vascularized network was established in the silicone chamber on day 28 post-implantation. The time of the blood glucose returningto normal level in diabetic mice was negatively correlated with the time required for pre-vascularization and the number of implanted islets. No islet grafts implanted in the silicone chamber and treated by anti-CD45RB survived for long term. However, one of seven (14.3%) grafts survived for long term, which was not significantly different from the transplantation under the renal capsule group (n=8, MST > 71 days,P> 0.05). The tissue-engineered pre-vascularization network markedly extends the survival time of the islet grafts before transplantation. The transplantation of the xenogenic pancreatic islets into the vascularized silicone chamber might be a promising method in the future clinical application.

ObjectiveTo observe the intraoperative techniques of artificial nasolacrimal duct and evaluate the effectiveness.MethodForty-nine cases(56 eyes) ofchronic dacryocystitis underwent artificial nasolacrimal duct placement.The artificial nasolacrimal duct was dilated and the stent was retrogradely placed.Dacryocystography was performed before operation.ResultsArtificial nasolacrimal duct placement was technically successful in 53 eyes(94.64％),improvement rate was 3.57 ％ (2/56).The total effective rate was 98.21％(55/56) after operation.ConclusionInterventional artificial nasolacrimal duct placement is a safe,simple and effective method for the treatment of chronic dacryocystitis.

Gallbladder cancer is a disease associated with high mortality. Improvement of early diagnosis is of great significance to prolong the survival. Risk factors for gallbladder cancer include gallstones, cholelithiasis, anomalous pancreaticobiliary junction, focal mucosal microcalcifications, and et al.Advances in endoscopic ultrasonography, magnetic resonance cholangiopancreatogram and helical computed tomography have enhanced preoperative diagnosis of gallbladder cancer. Understanding the characteristics of gallbladder cancer with the help of multiple imaging modalities can facilitate accurate diagnosis and may also help in sorting patients to undergo extended resection or an alternative therapy. Resection is currently the most effective and only potentially curative treatment for gallbladder cancer.However, owing to its non-specific symptoms, gallbladder cancer patients often suffer from late diagnosis, and few patients are suitable for surgery. Other treatment strategies such as chemotherapy, radiotherapy, percutaneous biliary drainage, palliative surgery are used in patients with advanced gallbladder cancer.For jaundiced gallbladder cancer patients, preoperative biliary drainage is still under debate. Since biliary inflammation adversely affects the prognosis of gallbladder cancer patients,antibiotics with high concentration in bile is recommended for selected patients. Palliative treatment and molecular target therapy are promising for patients with inoperable gallbladder cancer.

Objective To evaluate the curative effect of procedure for prolapse and hemorrhoids.Methods To retrospectively anabpe the clinical data of 61 patients with procedure for prolapse and hemorrhoids of Inner Mongolia medical school affiliated hospitals form 2002 to 2009.Results The average time of operation was 20～30 minutes.After operation,2 cases appeared urine retention,2 cases appeared bleeding and 1 case appeared prolapse lightly.The average time of being hospitalized was 5 days.The curative effect of allpatients was well after 1～6 years follow-up.Conclusion It was a safe and effective method to use procedure for prolapse and hemorrhoids,but the longterm curative effect of it still need further observation.

Objective To estimate the quality and efficacy of the academic thesis of compound anisodine in traumatic optic neuropathies(TON)treatment. Method We searched Chinese database last or"blunt trauma"as key words,and analyzed them using the standard of evidence-based medicine(EBM).Result 6 RCTS with a total of 415 eyes included are retrieved,and the OR value is 6.54 with a 95%CI of[4.14,10.35],P＜0.00001,the difference is significant;sub-category analyses are made and both show significant difference(P＜0.0001). Conclusion The existing evidence supports that prognosis of TON is better when compound anisodine are adopt in treatment,and this effect is significant in steroid treatment.Compound anisodine can be used alone for TON treatment.However.because there are only 6 thesis are retrieved and all of them have methodological short-comings,the evidence is not convincing.There is an urgent need of well-planed,large-scale and multiple-center studies to assess the role of compound anisodine in traumatic optic neuropathies treatment.

Objective To explore the effect of Tie-2 small interference RNA(siRNA)treatment in human hepatoma transplanted subcutaneously in nude mice.Methods Tumor cells were implanted in the hind flank of male nude mice of 6 weeks.Tumor-bearing mice were divided into two groups(gene therapy group and control group)and injected intra-tumorally with Tie-2-siRNA/Lipofectamine and saline/Lipofectamine respectively.The tumor volume and weight,serum AFP and microvessel density(MVD)and the histological change of the tumor were tested after gene therapy.Results The growth inhibitory rates in gene therapy group were 26.94%,53.01% and 68.91% on day 4,7 and 10 after gene therapy respectively.The tumor volumes of gene therapy group(118.47,111.57 and 104.59 mm3)were smaller than those of the control group(162.17,237.46 and 336.41 mm3)respectively(P

Alcohol is widely abused in contemporary social life, which has become a serious medical and social problem because it hurts human health and endangers public safety. Recent re-search has developed several active substances that can effective-ly improve or treat this syndrome via affecting the mesolimbic do-pamine nervous pathway to dampen rewarding effectiveness in-duced by ethanol. This paper reviews the progress in near-term studies of alcoholism-intervening agents, aiming at providing ref-erences for related mechanism exploration and drug development.

Objective:To explore the relationship between Jiegu pastes and blood biochemical indices and the expression of cal-catonin gene related peptide( CGRP) in the process of fracture healing. Methods: The tibial fracture model of rats was established, and the rats were randomly divided into five groups:the blank control group, model control group, positive control group, Jiegu paste at low dose group ( L, 1 g·ml-1 , calculated by raw materials) and at high dose group ( H, 2 g·ml-1 , calculated by raw materials) with 60 ones in each. The blank control group and the model control group were given 2 ml saline, the positive control group was given 2 ml concentrated dipsacus bone mixture, and Jiegu paste L group and H group was given 2 ml concentrated solution at low concentra-tion and high concentration, respectively, and all the groups were with the following regimen: ig,qd, for 28 days. The blood samples and callus specimens were obtained on the 7th, 14th, 21st and 28th day after fracture. Blood biochemical indices were detected, and HE staining and immunohistochemical staining were performed. Results: Compared with those in the model control group, the serum calcium and phosphorus ion concentration in Jiegu paste H group significantly increased on the 14th and 21st day, and the serum AKP significantly increased on the 14th and 28th day (P<0. 05). The expression of CGRP in Jiegu paste H group was significantly higher than that in the control group (P<0. 05). There were no significant differences between H group and the positive control group (P>0. 05). Conclusion:Jiegu paste can achieve the purpose of promoting fracture healing by improving the activity of serum AKP, in-creasing the concentration of calcium and phosphorus and regulating the activity of neuropeptide CGRP.

This paper digested some papers that were published from January to October,2009,and concerning about Chinese schistosomiasis control and research of schistosome,covering ectopie schistosomiasis,antischistosomal drugs and molluseieides,vector biology,epidemiology,etc.