Objective: To understand the prevalence of latent tuberculosis infection (LTBI) among college freshmen in Chaoyang District of Beijing from 2013 to 2015, and to provide evidence for prevention and control of tuberculosis in schools. Methods: The latent tuberculosis infection was identified through a tuberculin purified-protein derivative (TB-PPD) skin test. TB-PPD test results of 65 233 college students in the Chaoyang District during 2013 to 2015 were collected and stored, by using descriptive statistical analysis. Results: Among the 65 233 freshmen, 63 135 were effective PPD test results, 11 754 were PPD positive, 2 720 were PPD strong positive, the LTBI status: the PPD positive rate was 18.62% and the PPD strong positive rate was 4.31%. The positive rate and the strong positive rate in male (19.56%, 4.76%) were higher than that in female(17.87%, 3.95%)(χ2=29.37, 24.29), in ethnic minorities were higher than that in Han Nationality (18.25%,4.16%) and in Tibetans (29.45%, 8.69%) were the highest(χ2=102.01, 70.04), among freshmen from the western region (21.88%, 5.11%)were the highest and from Beijing city (16.25%, 3.46%) were lower (χ2=220.63, 99.03, P<0.01), among those with BCG scar (18.94%, 4.34%) are slightly higher than those without BCG scar(16.96%, 4.08%) (χ2=48.46, 10.43, P<0.01). 19 cases of tuberculosis patients were found, the prevalence rate was 29.13/100 000 (19/65 233), of which 68.42% (13/19) were found through the strong positive for PPD. Among the strong positive students (2 707), there were 1 433(52.94%) who received preventive therapy, and during the follow-up two years, 7 were newly diagnosed with tuberculosis, the annual incidence rate was 129.29/100 000 (7/2 707). Conclusion Freshmen from high-risk areas of tuberculosis are the focus of tuberculosis screening, for which we should continue to carry out PPD screening. Those with latent tuberculosis infection are high-risk groups of tuberculosis, and should be paid more attention in the prevention and control of tuberculosis in schools.