To investigate the efficacy and safety of treatment of rabbit knee joint toughness with autogenous synovium transplantation, we carried out the experiment at the experimental center of Department of Surgery, the Medical College of Xianning University between October 2003 and October 2005. Sixty black rabbits aged 6-10 months were selected and the lateral synovium of joint was resected to establish the artificial knee joint toughness models 4 weeks later. Then the rabbits were randomly divided into simple relaxation group (SR) and synovium transplantation group (ST) with 30 animals in each group. The latter group was subjected to antogenous synovium transplantation besides the relaxation of knee joint toughness. At the end of the 8th week, the ranges of motion of knee joint of the SR group: 16 cases were 0?-20?, 10 were 20?-40?, 4 were 40?-60?, and 0 was 60?-90?. The range of the ST group: 2 cases were 0?-20?, 8 were 20?-40?, 16 were 40?-60?, and 4 was 60?-90?. There were significantly differences between the two groups (P

ObjectiveThe investigate the action of self-care in geriatric type 2 diabetes patients.Methods96 geriatric type 2 patients were investigated through questionnaire. Results47 patients determined their blood sugar once or twice a week. 68 cases took medicine strictly.56 cases were good on alimentary control. 52 patients did exercise more than 1.5 h every day. The patients below 75 age old understood more about diabetes than that upward of 75.ConclusionTo improve the level of self-care on geriatric type 2 patients, the health care education must be done.

Objective:To clone interleukin 4 cDNA of Tibet pig.Methods:To amplify cDNA,using RT PCR from the total RNA from the lymphocyte stimulated by 10 ?g/ml Con A in vitro,to subclone into pMD T vector,and then to determine it by digesting and sequencing.Results:The cloned Tibet pig interleukin 4 was 98% homologus to the necleotide acids of Chenghua pig a special specie of pig in Sichuan province and 99% homologus to that of hybrid of Landrance with Chanbai pig.Conclusion:IL 4 of Tibet pig was successfully cloned from the lymphocyte after stimulating 70 h with ConA.

To explore the effect of acupuncture at Neiguan (PC 6) on the cardiovascular functions in the patient with low pulse pressure syndrome. Methods: 49 cases were randomly divided into acupuncture group and medicine group. Acupuncture group was treated by acupuncture at bilateral Neiguan (PC 6) for 3 consecutive days, and the medicine group was given intravenous drip of Shenmai Injectio for 6 consecutive days. 20ml each day. Blood pressure, pulse pressure. SV, CO. LVWL, MAP, TPR. CCP, PAWP, BV, MHR and CMBV were examined before and after treatment. 23 healthy subjects were observed as controls. Results: The pulse pressure of patients before treatment was significantly lower than that of controls (P

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Tumor surface antigen human epidermal growth factor receptor 2 (Her2) is a type I receptor tyrosine kinase, which belongs to human epidermal growth factor receptor family. Her2-overexpression is associated with tumorigenesis and metastasis. Due to significant clinical effects, Her2-targeted cancer therapy especially therapeutic antibody has become the hot spot in the field of cancer treatment. Anti-Her2 antibody drugs include monoclonal antibodies, antibody-drug conjugates, bispecific antibodies and emerging "two in one" antibody. Based on structure and function of Her2, this review focuses on recent advances in active mechanisms and clinical researches of these antibodies.
Antibodies, Bispecific ; therapeutic use ; Antibodies, Monoclonal ; therapeutic use ; Humans ; Immunoconjugates ; therapeutic use ; Neoplasms ; drug therapy ; Receptor, ErbB-2 ; immunology

Objective To compare the clinical outcomes of gefitinib and erlotinib treating non-small-cell lung cancer (NSCLC)with epidermal growth factor receptor (EGFR)mutation in either exon 19 or 21 . Methods A total of 242 patients diagnosed as NSCLC with EGFR mutation in either exon 19 or 21 from May 201 3 to December 2014 in our hospital were chosen in this study.According to age,sex,smoking history,eastern cooperative oncology group performance status and types of EGFR mutation,all the patients were matched to 121 pairs,and randomly divided into group A and B.Patients in group A received gefitinib treatment,and those in group B received erlotinib treatment.Based on the response evaluation criteria in solid tumors (RECIST),overall response rate (ORR),disease control rate (DCR),progression-free survival (PFS)were assessed.To assess the independent risk factors for PFS by univariate and multivariate Cox regression analysis.The subgroup analysis was performed for the 63 NSCLC patients using these two drugs as the first-line treatment.To evaluate the adverse drug reactions and quality of life between A and B groups.Results The median PFS of group A and B were 11 .6 months and 9.5 months,respectively,with no significant difference (HR =0.39,P >0.05).The ORR and DCR in the two groups were 76.9%,74.4% (χ2 =1 .03,P =0.58)and 90.1 %,86.8% (χ2 =1 .46,P =0.31 ). The independent risk factors of poor PFS were ECOG PS≥2 (HR =2.60,95%CI:1 .54 -4.43,P =0.001 )and non-adenocarcinoma (HR =3.61 ,95%CI:1 .54-8.66,P =0.003).For patients receiving these two drugs as the first-line treatment,there was no significant difference between two groups in overall response rates (76.6% vs. 90.2%,χ2 =0.83,P =0.12)and median PFS (11 .6 months vs.14.4 months,HR =0.59,P >0.05).The adverse drug reactions were significant differences in emotion function (F =10.27,P =0.03),diarrhea (F =10.24,P =0.03)and pain (F =9.02,P =0.04).After receiving drug treatment,the quality of life scores were improved,and most of the differences were statistically significant between A and B groups(P <0.05). Conclusion As for NSCLC with EGFR mutation in either exon 1 9 or 21 ,both gefitinib and erlotinib are well tolerated and have similar clinical effectiveness.

Intraperitoneal chemotherapy is a useful treatment of advanced gastric cancer.Following intraperitoneal chemotherapy, the concentration of drugs in the peritoneum and in the portal vein was high, lasting and sustained.Early post-operation chemotherapy showed better results.This article will summarize the newest research progress of intraperitoneal chemotherapy in the treatment of advanced gastric cancer.

Objective To identify the potential correlation of serum 25-hydroxyvitamin D3 [25 (OH)D3]and C-reactive protein (CRP) with coronary artery disease.Methods We measured the serum 25 (OH)D3 and CRP levels in 416 suspected coronary heart disease patients who underwent coronary angiography.Gensini scores were used to assess the severity of coronary stenosis.Based on the results of coronary angiography,the 416 patients were divided into normal group (n =246),stable angina group (n =110),and acute coronary syndrome (ACS) group (n =60).Results The serum levels of 25 (OH) D3 in the normal group and stable angina group were significantly higher than that in the ACS gropu [(33.15 ± 20.95) nmol/L vs.(15.55 ±5.7) nmol/L,(28.93 ± 16.45) nmol/Lvs.(15.55 ± 5.7) nmol/L,bothP＜0.001].TheCRPlevelin the normal group was significantly lower than those in the stable angina group and the ACS group [(4.33 ± 0.12) rng/Lvs.(5.68 ± 0.25) mg/L,(4.33 ± 0.12) mg/Lvs.(5.73 ± 0.31) mg/L,bothP＜0.001].The serum 25 (OH)D3 was not correlated with CRP or Gensini score in the normal group.In the ACS group,the serum 25 (OH) D3 was negatively correlated with CRP level and Gensini score (r =-0.026,P =0.045 ; r =-0.256,P =0.048),while CRP level was positively correlated with Gensini score (r =0.459,P ＜0.001).Inthe stable angina group,the serum 25 (OH)D3 was also negatively correlated with CRP level and Gensini score (r =-0.211,P =0.027; r =-0.208,P =0.029),and the latter two were positively correlated (r =0.574,P ＜ 0.001).Conclusions Low serum 25 (OH) D3 levels are associated with coronary artery stenosis.Combining with CRP,it may be involved in the pathogenesis of coronary heart disease through inflammatory mechanism.

Objective To study the prevalence of thyroid diseases in systemic lupus erythematosus (SLE) patients and to analyze the prevalence of subclinical hypothyroidism (SCH) with lupus nephritis (LN).Methods A total of 813 hospitalized SLE patients were retrospectively analyzed.The demographic,clinical and biochemical data were collected.The prevalence of different thyroid diseases was calculated.Among all patients,83 patients with SCH and 562 patients without any thyroid diseases were recruited according to thyroid hormone,thyroid stimulating hormone (TSH) and anti-thyroid antibodies levels.Case control study was performed to explore the potential risk factors for SCH in SLE.T test,Mann-Whitney U test,x2 test and Logistic regression analysis were used for statistical analysis.Results Among the 813 patients,13 (1.6％) had clinical hypothyroidism,83(10.2％) had SCH,13 (1.6％) had central hypothyroidism,27(3.3％) had autoimmunethyroid disease (AITD),10 (1.2％) had hyperthyroidism,95 (11.7％) had euthyroid sick syndrome (ESS) and 11 (1.4％) had nodules.SCH was more frequent in patients with LN (13.7％,50/366) than those without LN [7.4％(33/447),x2=8.654,P＜0.01].Meanwhile,prevalence of LN was also significantly higher in SCH group in case control study [60.2％(50/83) vs 42.9％(241/562),x2=8.800,P＜0.01].Besides,SLE patients with SCH had more severe proteinuria,hypoalbuminemia,and hyperlipidemia,which were complications of LN.In addition,the SCH group presented significantly higher anti-dsDNA antibody positive rate [50.6％(42/83) vs 33.7％(212/562),x2=5.026,P＜0.01].In Logistic regression models,24-hour urine protein and serum creatinine was retained as independent correlated factors with SCH after adjusted for demographic variables,risk factors,and other potential confounders.The presence of SCH was associated with increased 24-hour urine protein levels,occurring in 10.4％ of subjects with 24-hour urine protein ≤ 150 mg,11.9％ with 24-hour urine protein 150.1-1 500 mg,17.2％ with 24-hour urine protein 1 500.1-3 500 mg,and d24.4％ with 24-hour urine protein ＞3 500 mg (P＜0.05 for trend).In addition,when eGFR ≥30 ml·min-1· 1.73 m-2,the prevalence of SCH was increased as eGFR decreased:occurring in 12.8％ with eGFR ≥90 ml·min-1· 1.73 m-2,12.6％ with eGFR 60-89.9 ml·min-1· 1.73 m-2 and 20.0％ with eGFR 30-59.9 ml ·min-1· 1.73 m-2.Conclusion Thyroid diseases are common in SLE patients,and SCH is closely related with LN and disease activity.