BACKGROUND:Studies have shown that emodin protects against intestinal ischemia-reperfusion injury by inhibiting the release of inflammatory factors. OBJECTIVE:To investigate the effect of emodin on renal ischemia/reperfusion injury after bone marrow mesenchymal stem cel transplantation. METHODS:Forty Sprague-Dawley rats were randomized into five groups (n=8 per group):sham group, model group (renal ischemia/reperfusion injury group), bone marrow mesenchymal stem cel group (cel transplantation group), low-dose emodin group, high-dose emodin group. Rats in the latter four groups were pretreated with or without different concentrations (30 and 60 mg/kg) of emodin for 7 days, and then were subjected to clamping bilateral renal pedicles for 45 minutes, fol owed by injection of 1 mL bone marrow mesenchymal stem cel suspension. Six hours after reperfusion, the pathological changes of renal tissues were examined by hematoxylin-eosin staining;the mRNA levels of tumor necrosis factorα, interleukin-6, interleukin-18, TLR2 and TLR4 detected by real-time fluorescence quantitative PCR;and the protein expression of COX-2, ICAM-1 and iNOS determined by western blot. RESULTS AND CONCLUSION:Compared with the sham group, rats in the model group showed obvious features of severe acute tubular damage and inflammatory cel infiltration. In the cel transplantation group, tubular epitheliael cel s were partial y lost with some inflammatory cel s infiltrated in the renal interstitium. In the emodin groups, the tubular lumen was practical y intact with little renal interstitial inflammatory cel s. Compared with the sham group, a significant increase in the mRNA levels of tumor necrosis factorα, interleukin-6, interleukin-18, TLR2 and TLR4 as wel as in the protein levels of COX-2, ICAM-1 and iNOS was found in the model group (both P<0.05). However, bone marrow mesenchymal stem cel transplantation attenuated this ischemia/reperfusion-induced increase in the expression of the above-mentioned factors (P<0.05). Furthermore, the effects of bone marrow mesenchymal stem cel transplantation were further augmented by pretreatment with emodin in a dose-dependent manner. These findings suggest that emodin can enhance the protective effects of bone marrow mesenchymal stem cel s on renal ischemia/reperfusion injury.

Objective To study the effect of treating fatty liever with Tiaozhisugan decoction combined with simvastatin. Methods A total of 60 cases with fatty liver were randomly recruited into a control group and a treatment group.The control group was treated with simvastatin, vitamin E, and pioglitazone, while the treatment group was additionally treated with Tiaozhisugan decoction on that basis. Results The total effective rate was 93.9% and 79.6% in the treatment group and the control group respectively, the difference showing statistical difference (χ2=2.586, P＜0.01 ) .Conclusion It was effective to treat fatty liver with Tiaozhisugan decoction combined with simvastatin.

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Child ; Child, Preschool ; Enuresis ; physiopathology ; therapy ; Female ; Humans ; Male ; Treatment Outcome

Objective To observe the clinical effects of treating chronic type B hepatitis with Gankang Granule combined with poly I-C and immune ribonucleic acid. Methods A total of 100 cases of chronic type B hepatitis were randomly recruited into a control group and a treatment group, 50 cases in each group. The control group was treated with poly I-C and immune ribonucleic acid, while the treatment group was treated with Gankang Granule on the basis of the control group. Results Symptoms in both groups were improved. 23 eases in the treatment group showed negative value of HBeAg,contrasting to 13 cases in the control group. 38 cases in the treatment group turned to be negative value of HBV- DNA, higher than the number of 23 cases in the control group. The treatment group demonstrated a significantly better therapeutic results than the control group (χ2 value was 6.267 and 6.345 respectively, both P＜0.05). Conclusion Gankang Granule combined with poly I-C and immune ribonucleic acid can obviously improve hepatic functions and symptoms of chronic type B hepatitis,shorten the course of disease, and increase the rate of negative value of HBeAg and HBV- DNA.

Adult ; Female ; Foreign Bodies ; surgery ; Humans ; Neck ; Oropharynx ; surgery

Objective To investigate the therapeutic effect ofHuoxue-Buqi-Qutan decoction for vertigo due to vertebrobasilar insufficiency.Methods A total of 120 patients with vertigo due to vertebrobasilar insufficiency (VBI) were randomly divided into a treatment group and a control group, with 60 patients in each group. The treatment group was treated withHuoxue-Buqi-Qutandecoction and the control group was treated with nimodipine injection. The clinical efficacy, the change of strength before and after treatment were observed; the mean blood flow velocities of the vertebrobasilar arteries were detected with transcranial Doppler.Results After the treatment, the total effective rate was 91.7% (55/60) and 80% (48/60) in the treatment group and the control group, respectively, with significant difference (χ2=12.234,P=0.001). The mean blood flow velocities of the basilar artery, left and right basilar arteries were significantly increased in both groups (tvalues were 2.909, 4.558, 5.424, 9.831 in the treatment group, and 8.388, 11.191 in the control group, allP<0.01), the improvements of the mean blood flow velocities in the treatment group were significant better than those in the control group (tvalues were 7.385, 6.940, 3.890, allP<0.01). The vertigo grade was improved in both groups after the treatment than those before the treatment, while the proportion of grade 0 and grade 3 in the treatment group was significantly better than the control group (χ2 values were 8.298, 4.5472,P=0.004, 0.033).ConclusionHuoxue-Buqi-Qutan decoction can obviously improve the vertebrobasilar blood supply and the vertigo grade in patients with vertigo due to VBI, and then improve the clinical efficacy.

Objective To investigate the technical success rate of operation,safety,efficacy and clinical value of super-selec-tive uterine arterial embolization(SSUAE)in the treatment of massive postpartum hemorrhage.Methods The clinical data of 65 patients with massive postpartum hemorrhage who were not cured by medical treatment were retrospectively analyzed.Forty-one patients who underwent successful SSUAE were set as research team (group A)and 24 patients who underwent hysterectomy were set as control group (group B).The indications,success rate of hemostasis,bleeding volume and postoperative complications were compared.Results In group A,1 1 patients were in a critical condition and couldn′t tolerate the hysterectomy (26.8%),success rate of hemostasis was 95.1% (39/41 ),and 2 patients who with a poor outcome of embolization underwent hysterectomy after SSUAE (4.9%).In group B,the success rate of hemostasis was 95.8% (23/24),there was no statistical significance of hemostasis success rate between the two groups (P >0.05).There was significant statistical significance between the two groups in average operation time and bleeding volume in surgery,which were 33.8 min and 61.5 min,2 1 55.5 mL and 3 21 5.7 mL respectively(P <0.01).No postoperative complications occurred in group A,but 3 postoperative complications occurred in group B,which had signif-icant statistical significance (P <0.01).Conclusion SSUAE performed its excellent advantages,such as wide indications,minimal trauma,high success rate of hemostasis,low complications and reach the purpose of reserving uterus.

Objective: To analyze the risk factors and the predictive value of LDL/HDL ratio in acute ischemic stroke recurrence.
Methods: A total of 143 patients with acute ischemic stroke treated in our hospital were studied. The patients were followed-up for 2 years, the risk factors and the predictive value of LDL/HDL ratio for stroke recurrence were analyzed.
Results: Single factor analysis indicated that lacunar infarction, hypertension, blood pressure, the levels of LDL, HDL, ratio of LDL/HDL, stroke scale score, life ability score were the risk factors of ischemic stroke recurrence. Multivariate analysis presented that hypertension, ratio of LDL/HDL, TG level were the independent risk factors for ischemic stroke recurrence. LDL and ratio of LDL/HDL were positively related to ischemic stroke recurrence, HDL was negatively related to ischemic stroke recurrence, P<0.05. The speciifcity and sensitivity of LDL/HDL for predicting ischemic stroke recurrence were at 81.16%and 95.65%respectively.
Conclusion: The predictive value of LDL/HDL ratio could help us to identify the patients with high risk of ischemic stroke recurrence which is important for the early clinical intervention.

Lennox-Gastaut syndrome ( LGS) is an globally recognized refractory epilepsy,also known as an age-dependent epileptic encephalopathy. It is characterized by multiple seizure types, mental retardation, and slow spike-wave complexes discharges or fast rhythmic bursts during sleep on electroencephalographic re-cordings. Many antiepileptic drugs were reported for the treatment of LGS,but it was difficult to compare their efficacy because of the small sample size and design limitations. Valproate,topiramate and lamotrigine are rec-ommended to be the first-line drugs by most guidelines or expert consensus,and the combination of valproate and lamotrigine or the combination of lamotrigine and topiramate should be preferred as early as possible for initial treatment of LGS in children. Recent progresses of medical treatment of LGS are reviewed and some optical re-gimes are proposed in this paper.