The animal experiments,clinical researches and epidemiological investigations indicated that genistein has the effect for prevention and treatment of osteoporosis,cardiovascular disease and cancer.Genistein can inhibit cellular and humoral immunity and can regulate cell apoptosis.The chemical structure,biological effects and the mechanism of genistein attracted much attention.The current research advance on genistein was summarized and the prospect on the theoretical significance,clinical importance was presented in this paper.

Hypothyroidism is one of the most common endocrine disorders in childbearing or pregnant women.Gestational hypothyroidism can increase the incidence rate of the variety of pregnancy complications and adverse pregnancy outcome.It also can make adverse effects on the fetus and neonate,such as death,placental abruption,fetal malformation,intrauterine growth retardation and preterm,low birth weight,neonatal respiratory distress syndrome,it can also cause abnormal thyroid function of neonate,severely can cause nerve,mental,physical and mental developmental abnormalities and even death.Therefore,it is recommended that childbearing women who are at high risk for pregnancy should strengthen to monitor pregnancy thyroid function and treat as soon as possible in order to reduce the adverse effects on offspring.

OBJECTIVE: To evaluate the current situation and trend of narcotic drug use in our hospital where the authors work in order to provide the reference for scientific management and rational use of these drugs. METHODS: The yearly amount of narcotic drugs administered in the whole hospital, the yearly amount in the separate departments, as well as drug expenditures and ratios between January 2002 and November 2005 were added up and analyzed statistically. RESULTS: The amount of bucinnazine use dominated in the first. The amount of morphine for oral use was increasing year by year. The amount of fentangl transdernal patch use was also bigger and increasing rapidly. However, the amount of pethidine and morphine for injection use was decreasing. CONCLUSION:Analgetics for oral and transdermal use will be the main categories of analgetics in the future.

Vertical root fracture (VRF) is different from odontoclasis caused by trauma or injury. VRF is defined as a complete or incomplete fracture of roots caused by long-term and persistent stimulus, such as excessive forces from mastication or occlusion, improper tooth anatomy, and tooth treatment accident. Early diagnosis of VRF is essential to prevent the absorption of alveolar bone, thereby improving prognosis. Radiographic examination is the most common and effective evaluation method for VRF. Cone beam computed tomography can provide three-dimensional information for fracture details, which are more precise than traditional periapical films. In this paper, we present the radiographic features, differential diagnosis, and new treatment techniques for VRF.
Cone-Beam Computed Tomography ; Humans ; Tooth Fractures ; Tooth Root

Diagnosis, Differential ; Female ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Leukemia, Myeloid, Chronic-Phase ; genetics ; Lymphoma ; classification ; diagnosis ; pathology ; Male ; Neoplasms ; genetics ; metabolism ; pathology ; Oncogene Proteins ; metabolism ; Prognosis ; Prostatic Neoplasms ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Translocation, Genetic ; Tumor Suppressor Proteins ; metabolism

Objective To explore the changes of T-lymphocyte subsets,D-Dimer and immunoglobulin in mycoplasma pneumonia children and its significance.Methods The levels of T lymphocyte subsets (CD3 +、CD4 +、CD8 +、CD4 +/CD8 +)in 50 children with mycoplasma pneumonia (MPP Group)and 50 healthy children were detected by flow cytometry;The levels of D-Dimer were detected by immunoturbidimetry;The levels of serum immunoglobulin were detected by immune turbidimetric method.Results The results of T lymphocyte subsets:Compared with normal control group, CD3 +and CD4 +peripheral blood cells in MPP group were significantly declined(P<0.001)、CD8 +cells were increased slightly,but with no significant difference、CD4 +/CD8 +ratios were decreased slightly.The results of D-Dimer:Compared with normal control group,the levels of D-Dimer in MPP group were significantly escalated(P<0.001).The results of immunoglobulin:IgA and IgMin MPP group were both significantly higher than those of normal group,but IgG was not different from that in normal group.Conclusion Children with mycoplasma pneumonia have immune disorder,which may generate abnormal clotting.Therefore,besides the conventional anti-mycoplasma pneumonia treatment,we need to detect the levels of D-Dimer according to the clinical symptoms and disease progression and strength the regulation and treatment of the immune system.

Objective To study the T Lymphocyte Subsets and the cytokines in the newborns with infectious diseases. Methods Twenty cases of neonatal bacterial pneumonia (bacterial group), 15 cases of rotavirus enteritis (virus group) and 20 newborns with jaundice (control group) were recruited in this study. The peripheral CD4+T Cells and lymphocyte subsets were assessed by flow cytometry. Results The IL-4 level was significantly different among different groups (F=3.39, P=0.041). The levels of IL-17 and IFN-γdid not differ signiifcantly among different groups (F=0.28 and 1.24 respectively, P>0.05). The IL-4 level was higher in bacterial group than that in virus group and control group (P<0.05). The percentages of CD3+, CD4+, CD19+and NK cells and the ratio of CD4+/CD8+ had significant difference among different groups (F=3.30-26.69, P<0.05). The percentages of CD3+and CD4+cells and the ratio of CD4+/CD8+were lower in bacterial group and virus group than those in control group (P<0.05). The percentage of CD19+cells was higher in bacterial group and virus group than that in control group (P<0.05). The percentage of NK cells was lower in virus group than that in bacterial group and control group (P<0.05). Conclusions The pattern of cytokines level is different in newborns with infection caused by different pathogens. Newborns with infectious diseases have immune dysfunction and Th2-dominated imbalance. The low percentages of T lymphocyte subsets indicate the depressed cellular immunity after infection, which may result in atypical symptom and prolonged disease course.

Objective To investigate the changes of atherosclerosis and vascular tissue after pecutaneous tranluminal angioplasty (PTA) ,and evaluate the role of serum lipid ,lipoprotein (a) [Lp(a)] and tumor necrosis factor‐α(TNF‐α) in this progress .Methods Thirty New Zealand white rabbits with high fate diets were established as observation group ,and 10 rabbits with normal feed were set as control group .Blood biochemistry test was used to detect the levels of serum TC ,TG ,HDL‐C ,LDL‐C ,Lp(a) and TNF‐αbefore the experiment ,after 2 weeks feeding and 1 month ,2 months ,3months after PTA .The correlation among atherosclerosis and Lp(a) ,TNF‐a were measured by multiple logistic regression analysis .Opposite reference value was calculated with receiving operating characteristics (ROC) analysis .Results The levels of TC ,TG and LDL‐C were significant higher than baseline with the feeding time(P<0 .05) .The level of HDL‐C after 2 weeks high cholesterol diet feeding was lower than baseline (P<0 .05) .The content of TNF‐αat 2nd month after PTA was remarkable higher than baseline(P<0 .01) .ROC analysis showed that RR of TNF‐a was 1 .84(P<0 .01) and an area under the curve of 0 .871 for TNF‐α (95% CI:0 .811 -0 .956) compared to 0 .523 for Lp(a) (95% CI:0 .409-0 .720)regarding atherosclerosis .Conclusion The turbulence of lipid could be an important factor of atherosclero‐sis ,and the high level of TNF‐αmay take part in the process of repairing of arteriosclerotic vessel .

Objective To explore the correlation between miR-221/222 expression and the pathological features of thyroid carcinomas.Methods A total of thyroid tissue specimens was collected in thyroid surgery from Mar 2013 to Feb 2014.The expression levels of miR-221/222 were tested with quantitative real-time polymerase chain reaction (RT-PCR).The expression levels of miR-221/222 were analyzed in different thyroid carcinoma tissues.Results The expression levels of miR-221/222 were significantly over-expressed in thyroid carcinoma tissues compared to non-thyroid carcinoma tissues (F =9.358,P =0.0014).No significant difference was found among different groups of non-thyroid carcinoma tissues (F =1.367,P =0.839).The expression levels of miR-221/222 were significantly different in thyroid carcinoma specimens with different pathological features (P ＜ 0.05).Conclusions The expression levels of miR-221/222 were significantly over-expressed in thyroid carcinoma tissues.Both expression levels of miR221/222 were associated with extrathyroidal invasion,regional lymph nodes,and tumor staging.The expression levels of miR-221/222 might guide diagnosis,treatment,and prognosis of thyroid carcinomas.

Objective To investigate effectiveness of dexmedetomidine on preventing shivering of patients after spinal surgery.Methods A total of 78 cases with spinal surgery under anesthesia were randomly divided into study group(dexmedetomidine treatment) and the control group(saline treatment).Heart rate(HR)and mean arterial pressure were observed and recorded at 15,30,60 min after intubation,before and after extubation.Ramsay sedation score,chills grading,chills occur time and postoperative adverse reactions were recorded.Results In study group,HR were ((72.3 ± 5.2) time/min,(83.2 ± 4.5) time/min),mean arterial pressure were((72.7 ±5.0) mmHg,(90.3 ±7.9) mmHg) at before and after extubation,significant lower than that of control group((73.6 ±4.9) time/min,(88.9 ±4.9) time/min; (78.5 ±4.3) mmHg,(95.5 ± 14.9)mmHg;P＜0.05).The incidence of shivering (7.7％) in study group was significantly lower than the control group(30.8 ％,x2 =6.685,P =0.009).Ramsay sedation score at immediately,30 min,60 min into the PACU were (3.1 ± 1.0),(2.1 ± 0.4),(2.0 ± 0.3) respectively,higher than those of control group ((2.0 ± 0.4),(1.3±0.2),(1.1 ±0.3) ; F inter-group =12.543,P＜0.001;F inner-group =9.391,P ＜0.001;F interaction =5.972,P =0.023).The incidence of adverse reactions such as nausea and vomiting in the control group was higher than those of the study group(P =0.003),while the incidence of dry mouth in study group was higher (P =0.002).Conclusion Intraoperative dexmedetomidine can significantly reduce the incidence and degree of shivering in patients with orthopedic spinal surgery after general anesthesia.Dexmedetomidine is proved to be a more ideal drug to prevent shivering in orthopedic spine surgery after general anesthesia.