The animal experiments,clinical researches and epidemiological investigations indicated that genistein has the effect for prevention and treatment of osteoporosis,cardiovascular disease and cancer.Genistein can inhibit cellular and humoral immunity and can regulate cell apoptosis.The chemical structure,biological effects and the mechanism of genistein attracted much attention.The current research advance on genistein was summarized and the prospect on the theoretical significance,clinical importance was presented in this paper.

Hypothyroidism is one of the most common endocrine disorders in childbearing or pregnant women.Gestational hypothyroidism can increase the incidence rate of the variety of pregnancy complications and adverse pregnancy outcome.It also can make adverse effects on the fetus and neonate,such as death,placental abruption,fetal malformation,intrauterine growth retardation and preterm,low birth weight,neonatal respiratory distress syndrome,it can also cause abnormal thyroid function of neonate,severely can cause nerve,mental,physical and mental developmental abnormalities and even death.Therefore,it is recommended that childbearing women who are at high risk for pregnancy should strengthen to monitor pregnancy thyroid function and treat as soon as possible in order to reduce the adverse effects on offspring.

OBJECTIVE: To evaluate the current situation and trend of narcotic drug use in our hospital where the authors work in order to provide the reference for scientific management and rational use of these drugs. METHODS: The yearly amount of narcotic drugs administered in the whole hospital, the yearly amount in the separate departments, as well as drug expenditures and ratios between January 2002 and November 2005 were added up and analyzed statistically. RESULTS: The amount of bucinnazine use dominated in the first. The amount of morphine for oral use was increasing year by year. The amount of fentangl transdernal patch use was also bigger and increasing rapidly. However, the amount of pethidine and morphine for injection use was decreasing. CONCLUSION:Analgetics for oral and transdermal use will be the main categories of analgetics in the future.

Through summarizing medical consumable emergency safeguard mechanism in "5.12 earthquake relief",and combining with the related laws and regulations of the emergency safeguard,the characteristic of the medical consumable emergency safeguard mechanism are analyzed,and then the correlated proposal about which the medical consumable e-mergency mechanism in construction of military hospital emergencies are putted forward.

Active constituents in mycelium and fermcntation broth of Ganoderma lucidum were deter mined. Results showed that the amount of polysaccharides obtained from mycelium by 1mol/L NaOH extraction and hot-water extraction were 2% and 0.64% respectively, while the fer mentation broth contains 0.1% polysaccharide. The mycelium contains 17 kinds of amino aci ds, totalling at 14.7%. The fermentation broth contains 29 kinds of amino acids, totalling at 0.93%. The mycelium and fermentation broth contain 22 and 17 kinds of trace elements res pectively, predominately P, Fe, Zn and Ca

Vildagliptin,a dipeptidyl peptidase-4 (DPP-4) inhibitor,is always used in combination with metformin.Data from multiple clinical trials showed that vildagliptin and metformin combination therapy can improve HbA1c,fasting blood glucose,and postprandial blood glucose without weight gain,good gastrointestinal (GI)tolerance,and no increase in the incidence of hypoglycemia.Vildagliptin increases plasma levels of glucagon-like peptide-1 ( GLP-1 ).When glucose levels are above normal fasting levels,enhanced GLP-1 levels stimulate insulin secretion.Mefformin has been shown to increase insulin sensitivity and inhibit hepatic glucose production.The effect of vildagliptin to increase plasma levels of intact GLP-1 was enhanced in patients receiving concomitant metformin.

With the increasingly wide application of advanced science and technology, medical equipment, which reflects the overall strength of the hospital and medical activities, becomes more and more important. Medical equipment raises the level of medical care and increases the hospital income; while meantime, it is also bound to be accompanied by a range of purchase and management problems. The problems of medical equipment purchase and management in hospital are analyzed, and improvement opinions about equipment purchase, approval, use, maintenance and rejection are proposed in order to improve the actuality of equipment purchase and management.

Cationic colloidal gold (CCG) nanoparticles were used for labeling on the anioinic sites of living cells under two-photon fluorescence (TPF) microscope,and for delivering macromolecules into the target cells when irradiated by focused femtosecond laser pulses. 15 nm CCG nanoparticles which were made by conjugation with poly-L-Lysine,were attached on the anionic sites,especially on the membrane,of CHO-K1 cells because of their strong positive charge at physiological pH. Target cells labeled with cationic gold nanoparticles were imaged under TPF microscope,and lifetime images of the same targets were taken by time correlated single photon counting (TCSPC) technique in order to verify the fluorescence of the marker and the luminescence of the gold particles. The results shown that CCG nanoparticles first accumulated on the negatively charged sites of the membrane,then entered via endocytic pathway and attached anionic sites in plasma. A macromolecular 10 ku fluorescein isothiocyanate dextran (FITC-D) was added into the sample and the focused femtosecond laser of TPL microscope was employed to scan the target cells layer by layer. Typical laser power level used in biological imaging is about 3～5 mW. Here the laser power of scanning was below 5 mW in order to prevent photochemical damage of the fs-pulses alone and to localize effects to the nanoparticles on a nano-scale. After scanning the target cells under stack mode,macromolecular fluoresceins surrounding the cells was observed to cross the membrane and to diffuse in the cytoplasma. Comparing with the images before scanning,the two-photon fluorescence and fluorescence lifetime images revealed the delivery of FITC-D into target cells. Photothermal effects,which may be responsible for the permeabilisation,are highly localized in nanoscale and are not expected to cause damage exceeding the cell membrane. After extensive of laser scanning also cell death occurred. The ratio of the uptake of FITC-D and cellular death under different conditions were measured by flow cytometer. The results shown: with the increased scanning times or ratio of particles to cells,transfer efficiency increased first and decreased afterwards,but the ratio of cellular death went up all along.

CHN (the standards of skeletal maturity of hand and wrist for Chinese) is becoming more and more important in rencent years. At present, CHN bone age is applied in forecasting body height, menophania date, infantile endocrine diseases, judicial identification, orthopedics and so on. The clinical application of CHN bone age is introducted. In addition, the existing problems and future trends of bone age are pointed out.

Objective:To investigate whether the fish oil improves intestinal motility function during intra-abdominal sepsis and hypertension.Methods: 25 rats were divided into three groups.The rats were supplemented by gavage with: ①normal saline as control group(n=9);②fish oil(0.3%,n=8)and ③fish oil(0.6%,n=8)for 4 weeks before operation.The rats were killed on the 24th hour after operation.In vitro ileual smooth muscle contractions were recorded.Ileum sections were assessed pathologically.Immunopositivity of c-kit as markers of ICC-MY by confocal microscopy were evaluated.Results: In the bowel segment with NS,muscles showed a significant reduction in the frequency of contraction compared to that with FO.This change was associated with the changes of ICC-MY network.Conclusion:The intestinal motility may be protected by an enteral fish oil nutrition through attenuating injury of ICC-MY network.