Objective:To investigate the potential genes associated with cervical intraepithelial neoplasia (CIN) progression through mi-croarray expression profiling data analysis and bioinformatics approaches. Methods:mRNA expression microarray data related to CIN progression were screened from GEO database for the first time. They were re-analyzed by bioinformatics analysis. Results: Two mRNA expression microarray datasets were obtained from the GEO database. Pathway enrichment analysis of the common differen-tially expressed genes identified 3 signaling pathways associated with CIN progression, including Wnt, Endocytosis, and Vibrio cholerae infection. Fourteen differentially expressed genes were also identified. Biological annotation and text mining showed that 3 genes were directly related to CIN progression, and 9 other genes were associated with tumor progression and recurrence. GeneMania tool analysis demonstrated the protein interaction network formed between all the differentially expressed genes and the 24 reported genes. CCND2 and TGFBR2 formed direct interaction with many reported genes. Conclusion:Three signaling pathways and 14 differen-tially expressed genes were associated with CIN progression, as indicated by microarray data analysis results.

Intrabeam,a newly developed intraoperative radiotherapy in recent years,has many advantages such as accurate positioning,small size,easy to move and requiring a lower of protection.It is widely used in the treatment of breast cancer,colorectal cancer,lung cancer,bone metastases tumors and other malignancies.Intrabeam as a new way of intraoperative radiotherapy,is expected to provide more options for the treatment of a variety of neoplasms.

Objective:To evaluate the expression of EGFR and gene nm23H1 and their relation to the tumor oncogenesis and progress of cholangiocarcinoma.Methods:SABC immunohistochemistry was used to detect the expression of EGFR and nm23H1 in cholangiocarcinoma and cholangitis tissue.Results:The positive rate of nm23H1 in cholangiocarcinoma was lower than cholangitis(P

Objective To study age-related structural changes of proximal humerusin Chinese Han adults,for the application inage estimation.Methods Shoulder joint computed radiographs(CR) of 210 Chinese Han adults were sampled,with 124 males aging from 18 to 83 years old and 86 females aging 18 to 60 years old.Four observation indexes (the height of humerus bone marrow cavity,the change of trabecular bone in proximal humeral epiphysis,the change of trabecular bone in humeral macronodular and the change of trabecular bone in subacromial-clavicular bone marrow cavity) related with age were observed and scored.The regression equations of age estimation and change scores were established and tested statistically.Results Every index wasclosely correlated with the age changes inadults.The changes of trabecular bone in proximal humeral epiphysis and the changes of trabecular bone in subacromialclavicular bone marrow cavity have much better effects than the height of humerus bone marrow cavity and the changes of trabecular bone in humeral macronodular.The regression equations could predict the age of Chinese Han adults with satisfactory accuracy.Conclusion CR changes of the height of humerus bone marrow cavity,the changes of trabecular bone in proximal humeral epiphysis,the changes of trabecular bone in humeral macronodular and the changes of trabecular bone in subacromial-clavieular bone marrow cavity can bepracticallyusefulin age estimation for Chinese Han adults.

0.05). For the toxicity,Neutropenia and anemia were common in the three regimens. Thrombocytopenia was significantly mild on the NP regimen. For the PC regimen,gastrointestinal toxicity and nephrotoxicity were mostly mild,and peripheral neurotoxicity was most common. Conclusions:Efficacy was not significantly different between the three investigated regimens. These regimens could be use as first-line chemotherapy in advanced NSCLC. Toxicities of these regimens were different,so clinicians should apply the chemotherapy according to individual characteristics of patients.

Objective To observe inhibition rate of mouse mammary adenocarcinoma(EMT6) and inhibition effect on tumor angiogenesis of Panax notoginseng F.H. Chen and Cordyecps sinensis Sacc. powder. Methods BALB/C mice as bearing cancer animals with subcultured mouse-transplantable EMT6 cell train had accepted intervention treatment by Panax notoginseng F.H. Chen and Cordyceps sinensis Sacc. powder. In this way,inhibition rate of the mouse-transplanted EMT6 by the powder was recorded and the expression of microvessel density(MVD),vascular endothelial growth factor(VEGF) and tissue inhibitor of metalloproteinase-2(TIMP-2) in the tumor tissue after paraffin imbedding and section were detected through immunohistochemistry technique. Results The tumor inhibition rates of two designed dose groups were separately 48.95% and 37.64%. MVD and VEGF expressions of tumor tissue in the two group were significantly suppressed while the TIMP-2 expression increased. Conclusions Panax notoginseng F.H.Chen and Cordyceps sinensis Sacc. powder can restrain the growth of EMT6,and the pathway of this function is likely related to inhibiting tumor angiogenesis.

Histological characters of stems of Cirsium japonicum Fisch. ex DC. and C.chinense Gardn. et Champ. were described, compared and illustrated with line drawings. It was found that the following characters are important for disting uishing the two plants;(1) the type of stomata, vessels or fibres; (2) the presence or absence of secretory canal or tracheids.

Objective In order to provide the reasons for local injecting Xiaozhiling to treat malignant tumors. Methods 10 mice were subcutaneosly injected with S 180 Tumor cell to create tumor models and then Xiaozhiling 10ml/kg were intratumorly injected for 3 times.Finally,observation and comparison were made for the pathologic changes under optic and electronic microscopies. Results The results showed that Xiaozhiling had strong antitumor effect on S 180 tumor(adquate rate was 37.3%).Pathalogical exemination revealed that the tumor tissue necrosed obviously,some wesslincataneous cells breaked up, red blood cells extravasation,micro blood clots formed.No changes occurred in the control group. Conclusions Xiaozhiling could be a new drug for clinical treatment of malignant tumor.

Objective To evaluate the relationship between airway inflammation before treatment and asthma status and response to inhaled corticosteroids treatment in children with mild or moderate asthma. Methods Eighty-seven children diagnosed with mild or moderate asthma were enrolled as study group, 20 healthy children as control group. Sputum induction, cellular differential count, and the assaying of mediators in sputum supernatant were performed before treatment with corticosteroids. Eosinophil cationic protein were measured by enzyme-linked fluorescent assay, interleukin-8 and transforming growth factor-β_1 (TGF-β_1) were measured by enzyme linked immunosorbent assay. Pulmonary function tests were performed for small airway function on the baseline and methacholine bronchial provocation tests were performed to screen airway hyperresponsiveness. SPSS13.0 software was used to analyze the data. Results (1) Among the 87 patients, 64 patients were studied as eosinophil asthma (EA) group, 23 patients as non-eosinophil asthma (NEA) group according to the ratio of eosinophils in sputum. The percentages of inflammatory cells and level of ECP, IL-8 were of significant difference between the two groups (P < 0.05), other indexes as FEV_1% pred, PEF% pred, moderate-severe AHR%. small airway function were also of significant difference between the two groups (P < 0.05). (2) Patients in EA group showed significant improvement in pulmonary function, bronchial hyperresponsiveness and small airway function after treatment with inhaled corticosteroids compared with NEA group. (3) Multiple stepwise regression analysis showed that among the different baseline variants considered only baseline FEVl%pred, sputum eosinophil percentages (EOS%), sputum TGF-β_1 significantly correlated with the response to inhaled cortieosteroids, moreover, sputum eosinophil percentages had the closest correlation (β= 0.583, t = 6.214, P < 0.05). Conclusions There were different patterns of airways inflammation in children with mild or moderate asthma. Sputum eosinophilia was associated with asthma status. Low sputum eosinophils, low FEV_1%pred, high sputum TGF-β_1 before treatment predict poor response to treatment with inhaled corticosteroids. Evaluation of those baseline indexes may be helpful to an individualized therapeutic regime.

Hepatic epithelioid hemangioendothelioma is a rare and low-grade malignant tumor of vascular origin.Clinical symptoms and laboratory findings are unspecific.The definitive diagnosis can only be made by histopathalogi-cal investigation The primary treatments of choice are radical hepatic resection or liver transplantation. The role of differ-ent adjuvant therapies for patients with hepatic epithelioid hemangioendothelioma remains to be determined.