Objective: To study the effect of EEG biofeedback in the treatment of children with ADHD and comorbid tic disorder. Methods:14 children with ADHD and comorbid tic (criteria of DSM-IV) aged 7 to 14 received EEG biofeedback treatment (average 34 sessions). The outcome was evaluated with Conners Parent Symptom Questionnaire(PSQ),Rutter Questionnaire,Achenbach CBCL,and C-WISC-R,WMS,CPT before and after treatment. YGTSS was used to evaluate the severity of tic disorder. Results: Hyperactivity index in PSQ, the total score of Rutter, and hyperactivity factor in CBCL were greatly reduced after treatment (P

Objective:To explore the therapeutic effect,security and influencing factors of methylphenidate titration program,try to find the potential predicting index of therapeutic effect.Methods:This study was openly designed.The patients who were fit for DSM-Ⅳ diagnosis criteria of attention deficit hyperactivity disorder(ADHD) underwent systematic methylphenidate titration treatment until they attained the most optimal dose with best therapeutic effect and minimal side effects.The primary evaluative index of therapeutic effect was ADHD rating scale filled by teachers(ADHD-RS-Ⅳ),and secondary index included abbreviated Conners questionnaire filled by teachers,ADHD rating scale and Conners questionnaire filled by parents.The matching sample t analyses were used,then the differences of rating scale between pre and post treatment were compared.The potential influencing factors information of therapeutic effects including symptom severity degree when visiting,IQ,sensory integration capability and achievement of school etc.were collected.Finally,the correlation analysis was used for continuous variables andvariance analysis for dichotomization variables to analyze and check the factors' influence of declining scores of behavior scale.Results:The final prescribed daily dose for patients who completed dose titration was(17.3?7.7) mg on average,computed by kilo bodyweight was(0.45?0.11) mg/(kg?d).All the scale scores between pre and post treatment were significantly different(the pre-treatment score of ADHD rating scale completed by teachers was 30.25?9.16,and post-treatment score were 19.87?10.03,P

OBJECTIVE To investigate the trend of resistance to antimicrobial agents among clinical isolates of Escherichia coli and Klebsiella pneumoniae in 2006-2007.METHODS Agar diffusion test was used to analyze the changes in drug susceptibility of E.coli and K.pneumoniae from 30 hospitals in Anhui in 2006-2007.The resistant rate,intermediate rate and susceptibility rate of drugs were calculated according to the criteria in guidelines of CLSI(2007).RESULTS Carbapenems were the most active antimicrobial agents tested against E.coli and K.pneumoniae.Cefepime,ceftazidime,piperacillin/tazobactam and amikacin showed excellent activity against E.coli and K.pneumoniae.E.coli and K.pneumoniae were still resistant to cefuroxime,ceftriaxone,cefotaxime and aztreonam.ESBLs-producing E.coli strains accounted for 48.9% in the year of 2006 and for 47.2% in the year of 2007.ESBLs-producing K.pneumoniae strains accounted for 36.6% in the year of 2006 and for 40.0% in the year of 2007.CONCLUSIONS Imipenem remains highly active against E.coli and K.pneumoniae.

OBJECTIVE:To establish the method to determine the concentration of human plasma felodipine by HPLC-MS.METHODS:The HPLC separating system was adopted and the mobile phase was a mixture of methanol-water-acetic acid with a flow rate at1ml/min and split ratio at2∶1,the fixed phase was Hypersil C 18 column;The mass spectrometric de-tection system and AP-ESI positive ion model were adopted with the atmosperic pressure at0.054Pa(400psi),the protecting gas at N 2 10L/min,capillary voltage at4000V,the ion source temperature at100℃and the fragment voltage at220V,the ions collected by SIM were felodipine m/z411(M+Na),Nimodipine m/z441(M+Na).RESULTS:The linear concentration range for felodipine was0.2～50ng/ml with a minimum detection limit at0.15ng/ml,the recovery was above90.93%with both the intra-day RSD and the inter-day RSD at less than9.8%(RSD

To investigate the changes of drug resistance of pseudomonas aeruginosa ( PA) to common antimicrobial agents in recent 3 years in Anhui province. The average drug resistance rate was 15. 3%, 17. 0% for piperacillin/tazobactam, amikacin respectively, while the average resistance rate was higher for the third generation cephalospo-rin cefotaxime, ceftriaxone and ceftizoxime, which was between 42. 0% and 55. 0%. The average drug resistance rate was 25. 2% for imipenem. The resistance rates for ceftazidime, cefepime, ceftriaxone and ceftizoxime were sig-nificantly increased( P<0. 05 ) . The resistance of PA to the third generation cephalosporin and ciprofloxacin in-creased gradually, close attention should be paid to it in clinical practice.

At present,the technology to predict the response to neoadjuvant therapy with biomakers has been widely used in clinical practice.The approaches of biomarkers detection are various,including immunohistochemistry,detection of serum biomarkers conventional blood tests,gene expression profile analysis,single nucleotide polymorphisms,miRNAs,proteomics analysis.With the development of biotechnology,the technology of biomarkers detection is expected to become effective means in assessment of adjuvant therapy,risk,prognosis and individualization in esophageal cancer treatment.

ObjectiveTo investigate the characteristics of sexual behavior and HIV infections between normal men who have sex with men (MSM) and money boy (MB) in Jining city,so as to provide the basis for intervention.MethodsSentinel surveillance,VCT and respondent-driven sampling were adopted to recruit the target population,and the data was aualyzed with SPSS13.0.ResultsThe average age of normal MSM was 26.45 ± 5.8 and MB was 22.23 ± 3.3.The rates of single,marriaged and divorced about normal MSM and MB were 48.24％,45.53％,6.7％ and 85.4％,9.8％,4.9％ respectively.The educational level of normal MSM(74.8％ )was higher than that of MB(51.22％ ).MB group's rate of condom used(75.61％ ;51.22％ ) was higher than that of normal MSM ( 52.03 ％ ;32.79％ ).There was statistical significance in age,educational level,marital status and infecting rates of HIV between MB group and normal MSM group.ConclusionCompared with normal MSM,MB is the high risk population and bridge population of transmission of HIV.

FLIP is a protein containing death domain.Human beings have three subtypes of c-FLIPL,c-FLIPs and c-FLIPR,which can inhibit the apoptosis of a variety of tumor cells.c-FLIPL plays a dual role in the apoptotic signal.The expression level of FLIP not only decides the opening and closing of the apoptosis pathway but also achieve the conversion of cells in the apeptotic signaling and proliferative signaling pathway.The regulation of FLIP's expression is a multi-layered,involving multiple signaling pathways.FLIP will probably become an attractive death receptor signaling target of therapy.

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Objective To investigate the expression of Ptch-1 and Gli-1, hedgehog pathway-related genes in squamous cell carcinoma (SCC), and the effect of cyclopamine, a specific inhibitor of hedgehog signaling pathway, on the proliferation of a SCC cell line Tca. Methods Skin samples were resected from 42 patients with SCC and 10 normal human controls. Immunohistochemistry and in situ hybridization were employed to study the expression and distribution of Ptch-1 and Gli-1 in these specimens. Tca cells were incubated with cyclopamine (1, 2, 5, 10 μmol/mL) for 48 hours, or cyclopamine (5 μmol/mL) for 1-8 days. The same concentrations of lycopersicin served as the control treatment. Then, MTT assay was performed to detect the proliferation of Tca cells. A fraction of Tca cells were cultured in the presence of 5 μmol/mL cyclopamine for 72 hours followed by BrdU assay for the evaluation of cell growth and proliferation. Results A significant increment was shown in the expression of both Patch-1 and Gli-1 by immunohistochemistry (χ2= 5.656, 6.732, P<0.05, 0.01, respectively) and in situ hybridization (χ2=6.787, 9.600, respectively, both P<0.01) in SCC tissue compared with the control specimens. And both of them were predominantly distributed in the cytoplasm of SCC cells. As MTT assay revealed, cyclopamine notably inhibited the proliferation of Tea cells, and the effect increased with the concentration and action time of cyclopamine. Further more, the percentage of BrdU-positive cells was 26% in cyclopamine-treated Tca cells, significantly higher than that in the blank control cells (77%) and lycopersicin-treated cellls (72%). Conclusions Hedgehog signaling pathway is activated in the lesions of SCC, and inhibition of the pathway may facilitate the treatment of SCC.