Objective To observe the effect of adjusting high dietary profein intake on nutritional status of peritoneal dialysis (PD) patients. Method Thirty three PD patients whose daily protein intake (DPI) was over 1.2g/(kg? d) were included in study from July, 2005 to November, 2006. A recipe was given by a professional dietitian for every patient to reduce DPI meanwhile maintaining sufficient daily energy intake (DEI). DPI, DEI, serum albumin (ALB), lean body mass(LBM) and subjective global assessment(SGA) and the prevalence of hyperphosphatemia, hyperkalemia and metabolic acidosis were evaluated before and after 3 and 6 mo adjustment. Results At the end of observation, 28 patients were enrolled in the study. Their mean age was 61.37?12.25 yrs, 11 males and 17 females with dialysis period 8.8 mon (1～66 mo). DPI and DEI were significantly decreased during 6 mo (P=0.000). Nobody complained about gastrointestinal symptoms, and the quality and satisfaction of life during the study were not significantly different. Serum Alb at 3 rd month was higher than the start of study (36.22?2.79g/L vs 37.34?3.32 g/L,P

Six taxane diterpenoids were isolated from the stem bark of Tax us cuspidata Sieb. et Zucc.. On the basis of physical and chemical properties and spectra analysis,they were identified as 2'-de sacetoxyaustrospicatine(Ⅰ). taxol(Ⅱ),cephalomannine (Ⅲ), 2-deacetoxyl taxinine J (Ⅳ),taxinine B(Ⅴ) and 10-deacetyl-7-0-?-Dxylosyl-taxol (Ⅵ).

Objective To study the relationship between the cerebrospinal fluid (CSF) volume and intracranial measurement line in normal Chinese by useing CT data and computer technology.Methods By CT scan,to measure CSF volume and third ventricle maximum width,suprasellar cistern minimus width,lateral ventricle width in hypothalamic layer,caudate nucleus pitch and other index by age and gender in 178 normal adults.Results There were significant difference between male and female CSF volume in the groups of 14-20 years(23 cases),31-40 years(33 cases),41-50 years(32 cases) and ＞ 60 years (13 cases)(P＜ 0.05 or ＜ 0.01).The CSF volume between male and female had significant difference (t =3.302,P =0.001).The CSF volume and third ventricle maximum width had significant correlation (r =0.685).Conclusion The third ventricle maximum width is the most closely with CSF volume.

Objective To clarify the clinical features of solid pseudo-papillary tumor of pancreas. Methods Clinical data of 38 patients with solid pseudo-papillary tumor of pancreas were analyzed retrospectively. Results The clinical manifestations included a palpable abdominal mass, abdominal pain and discomfort. Tumor occured in 1 case younger than 10 years old, 11 cases between 11 years old and 20 years old, 7 cases between 21 years old and 30 years old, 9 cases between 31 years old and 40 years old, 5 cases between 41 years old and 50 years old and 1 case older than 50 years. Immunohistochemistry staining showed CgA( + ) in 6 cases,Vim( + )in 29 cases,AAT( + ) in 29 cases,Syn( + ) in 21cases,CD10( + ) in 34 cases,CD56( + ) in 37 cases, Ki-67 ( + ) in 36 cases and PR( + ) in 38 cases. Conclusion Solid pseudo-papillary tumor of pancreas is closely associated with gonadal hormone and reproductive system.

Objective To discuss the medication principles of diagnosis and treatment for insomnia from spleen and stomach; To provide references for clinical treatment for insomnia.Methods Relevant articles about diagnosis and treatment forinsomnia from spleen and stomach were searched in CNKI and Wanfang Data through computers, the data were input into Traditional Chinese Medicine Inheritance Supporting System (V2.5) and then association rules, apriori arithmetic, improved mutual information method, and complex system entropy cluster methods were used to analyze the medication principles of diagnosis and treatment for insomnia from spleen and stomach. Results Through screening, totally 87 prescriptions were included, involving 165 herbal medicine. Data mining results showed that there were 30 high-frequency herbal medicine, 19 high-frequency medicinal combinations, and 4 medicinal association rules. Evolution led to 13 medicinal combinations of 2 herbal medicine and 3 new prescriptions. Conclusion The main method of diagnosis and treatment for insomnia from the spleen and stomach is regulating qi activity of spleen and stomach. The features of common medicine are invigorating spleen to reduce phlegm, clearing stomach and calming the adverse-rising energy, and tonifying qi and soothing nerves, which can offer references for clinical treatment for insomnia.

Objective To investigate the changes and clinical significance of platelets aggregation rate of patients with coronary heart disease before and after percutaneous transluminal coronary stenting Methods There are 13 patients underwent coronary angiography and 20 patients underwent percutaneous transluminal coronary stenting Their blood samples were taken from the coronary sinus and coronary artery before and after interventional therapy Results The level of platelets aggregation rate in percutaneous transluminal coronary stenting group was higher after interventional therapy ( P

Objective To observe effects of senior neurogenic hypertension treated with microvascular decompression(MVD) Methods All 692 cases of senior cranial nerve disease with MVD were retrospectively analyze, 236 cases of preoperative neurogenic hypertension(prosopalgia 179 cases,facial spasm 48 cases, glossopharyngeal neuralgia 9cases)were treated with MVD Results In 236 cases,145 Patients had cure results(61 4%),36cases obvious effectiveness(15 3%),18 cases effectiveness(9 7%),32 cases ineffectiveness (13 6%) Two hundred and eight cases have been flowed up for 74 months average,133 cases had cure prognosis,30 cases obvious effectiveness,18 cases effectiveness,27 cases ineffectiveness Conclusion It is pathogenesis of neurogenic hypertension that oppressing cranial nerve roots and bulb by abnormal vascular tab leads to long term aching stimulation and emotional stress MVD is an effective treatment of neurogenic hypertension

Objective To study the effect of cumulus cell apoptosis on the structure of human oocyte cultured in vitro and to probe into the reason why cumulus cell apoptosis rate can be used clinically to predict the developmental potential of oocyte. Methods Cumulus-enclosed oocyte complexes (COCs) at the GV stage were cultured in vitro. The apoptosis rate of cumulus cells for every COC was evaluated with HE staining, DAPI staining and TUNEL labeling methods. Oocytes were divided into two groups according to their cumulus cell apoptosis rate. Structure of oocytes was observed under light microscope and transmission electron microscope. Mature oocytes at MⅡ stage were fertilized and cultured in vitro for two days. Then the embryos were evaluated according to their morphology under light microscope.Results The oocytes with low cumulus cell apoptosis rate had a normal structure and a promising developmental potential, while the oocytes with high cumulus cell apoptosis rate had deformed structures and a poor developmental potential. Some deformed oocytes had uneven perivitelline space. For oocytes with a small perivitelline space, the organelle development was slow compared with those with a large perivitelline space. Some had unevenly piled organelles. Secondary lysosomes and large space probably caused by the decopmounding of lysosomes were found in the ooplasm of these oocytes. The deformed oocytes appeared to have lots of swollen mitochondria with blurred cristae and membrane. Some of these oocytes had fractured first polar bodies and abnormally thick or thin zona pellucida. The cell junctions and microvilli of cumulus cells reduced as well. Conclusion The effect of cumulus cell apoptosis on the structure of oocyte cultured in vitro was revealed. The reason why cumulus cell apoptosis rate prognosticated the developmental potential of oocyte was demonstrated in the aspect of oocyte structure.

2?l 10% ricin were injected into the pelvic nerve of cats. After 3～5 survival days 20% HRP solution was injected into the ipsilateral lateral parabrachial nucleus, 2～3 days later the animals were perfused with paraformaldehyde and glutaraldehyde, the brain and spinal cord were removed and processed for light and electron microscopy. It was confirmed that the primary afferent terminals (degenerated) of pelvic nerve formed axo-dendritic and axo-somatic synapses with the retrograde labelled neurons projected to the lateral parabrachial nucleus which were located in the dorsal commissure nucleus (DCN), intermediolateral nucleus (IML) and lamina Ⅰ of sacral spinal cord. Hence, the secondary afferent pathway of pelvic visceral sensation, as. indicated at the ultrastructural level, might originate from DCN, IML and lamina Ⅰ of sacral spinal cord and project to the lateral parabrachial nucleus.

The Nissl and Golgi staining methods and electron microscopy were u(?)o investigate the architecture of dorsal commissure nucleus (DCN) of the sacral spinal cord in cat. A morphologically specific nucleus could be identified in the medial part of the lamina Ⅵ of dorsal horn. The nucleus located beside the dorsal funiculus at the lumbar level and gradually moved toward the midline of the dorsal gray commissure in the ventromedial direction as the dorsal gray commissure enlarged at the segments S_(1-2) and two ellipsoid nuclei was formed at the two sides of the midline. A large triangular nucleus was gradually formed by fusion of the two nuclei at S_3-Co_1. Golgi stain positive neurons could be classified into three types: (1) small-triangular neurons (60%); (2) fusiform neurons (30%); (3) oval and irregular neurons (10%). The cell organells of most neurons were plentiful. The cell nucleus was irregular and often with deep invaginations. A large number of free ribosomes and saccules could be found in the canals formed by the nuclear invagination. There were axo-dentritic, axo-axonic, axo-somatic synaptic contacts in the DCN with percentages of 89%, 6%, and 5% respectively. The serial synapse, axo-spinous synapse and crest synapses could also be found.