OBJECTIVE:To discuss the prospect of hospital preparation and to find out effective measures for its devel-opment.METHODS:The history of hospital preparation was reviewed,and the way for its survival was discussed on the basis of current situation and newly formed laws and regulations on preparations.RESULTS&CONCLUSION:Hospital preparation can survive and develop only by“controlling scale,guarantee quality,creating specialty and exploiting new drugs”.

The polymorphisms of VNTR loci D1S80 (pMCT 118), D17S30 (pYNZ-22 ) and ApoB3' in blood and tissues were detected by amplified fragment length polymorphism (Amp-FLP) technique,and was applied in the paternity testing cases. The discriminating power (DP) of D1S80,D17S30 and ApoB3'were 0. 962, 0. 956 and 0. 960 respectively. The cumulative probability of paternity exclusion (EPP) of the three VNTR loci was 94. 51 %,higher than that of conventional blood typings.

The membrane fluidity of mitochondria isolated from langendorff perfused rat hearts was studied with ESR spin labeling technique and TBA method was used to detect the content of lipid peroxide. After total ischemia for 40min followed by a 20min reperfusion the 10-DSA region of the mitochondrial membrane showed significantly increased S (0.1(?)2?0.006) and ?_c(7.13?0.09?10~(10)sec) values (i. e. a decrease of membrane fluidity)in comparison with aerobic control (S=0.103?0.007); ?_c=6.86?0.20?10~(-10) sec) (P0.05). In addition, we found that DS182 could remove O_2~? produced by O_2~? generating system. Our results have showed that DS-182 is a potent scavenger of superoxide. The protective effect of DS-182 on mitochondria during ischemia/reperfusion might be due to its antioxidative activity。

Calcium overload has been discovered in the ischemia and reperfusion injury of the heart recently, but there were less reports about changes in myocardial Ca distribution under this same condition by electronmicroscopy. Using the potassium-pyroantimonate method and X-ray microanalysis, we studied cytochemical calcium localization in the rabbit's myocardium at various time periods of ischmia with or without subsequent reperfusion. At the same time, we had the morphometric estimation of Ca granule volume density in the mitochondria by TAS(texture analysis system) and evaluated Danshen's protection for ischemia and-reperfusion injury. The results showed that mitochondrial Ca granule volume density increased significantly with the development of ischmia and post-ischemic reperfusion, especially during reperfusion following 40min of ischemia, however following 60min of ischemia, Ca granule volume density decreased than that observed following 40 min of ischemia. This meant that the limit time point for reversible and irreversible injury could be in the range of 40～60min of ischemia. Danshen pretreatment to the rabbits reduced Ca granule volume density significantly. This demostruted that Danshen acted as a calcium antagonist and contributed an outstanding protection to the myocardial calcium overload.

OBJECTIVE To evaluate the effect of AG490 on the invasion of Hep2 laryngeal carcinoma cells, and to investigate the role of STAT3 signaling pathway in human laryngeal carcinoma invasion.METHODS Janus kinase inhibitor AG490 was used to inhibit the activation of STAT3 signaling pathway of Hep2 cells.Hep2 cell invasion was detected by quantitative Matrigel invasion assay.The expression of p-STAT3 was measured by flow cytometry. RESULTS The activation of STAT3 signaling pathway and the invasion of laryngeal carcinomas Hep2 cell line were suppressed by AG490.The expression of p-STAT3 and the invasion cell number of Hep2 cell line in the group treated by AG490 for 24h decreased compared with that in control group(P

Nonalcoholic fatty liver disease (NAFLD) is associated with obesity, type 2 diabetes,dyslipidemia, hypertension, and metabolic syndrome.It likely represents hepatic manifestation of metabolic syndrome. The prevalence of NAFLD has been estimated to be 20％-30％ in the general population. However, it is much higher in type 2 diabetic patients. Several epidemiological studies indicate that NAFLD is linked to an increased risk of cardiovascular disease( CVD), which is independent of underlying cardiometabolic risk factors. This suggests that NAFLD may also be actively involved in the pathogenesis. The possible molecular mediators linking NAFLD and atherosclerosis(AS) include the release of pro-atherogenic factors by oxidative stress and inflammation as well as atherogenic dyslipidemia, abnormal adipose factors, and insulin resistance, finally aggravating progression of CVD. In this article, the relationship and possible mechanisms between NAFLD and AS are reviewed.

Objective To select the acceptor ingredients of Streptococcus mutans protein in the whole saliva through the interaction between adhesion and saliva protein of Streptococcus mutans. Methods After activation and liquid culture of Streptococcus mutans strains of international standards, we took Su-bonding from liquid culture of bacteria secretion in the process of liquid culture by using chilled ethanol extraction. Then su-bonded components underwent electrophoresis analysis, content determination and antigen detection. We collected 15 with high carries and 15 with no carries. Two hours after eating, the whole non-irritating saliva was collected, electrophoresis samples were prepared after freeze-drying, and then stains were separated with SDSPAGE. Western blot detection was used for adhesive protein and Streptococcus mutans' antibody composition. Results The ingredients of Su-bonding secreted on the surface of Streptococcus mutans were obtained in the cultured saliva. There were at least six kinds of components in the extraction of Western blot detection, not limited to the three known kinds of proline-rich protein. Conclusion The adhesion of Streptococcus mutants can be bound with the proteins of saliva. It may be the material basis in Streptococcus mutants adhered to tooth surface.

Objective To study of relationship between elderly people's alexithymia and mind-reading. Methods There were 108 old people over 70 years old, tested by mind-reading,cognitive ability and twenty-item Toronto alexithymia scale. Results There were significant difference in mind-reading(16.00±4.84, 19.36±2.91,t =3.38,P ＜0.01), memory span(5.80±1.42,7.18±1.13,t =4.28,P ＜0.01), working memory( t =4.05,P ＜0.01),processing speed( t =-4.80,P ＜0.01) between high and low alexithymia groups.Alexithymia scores were negatively related to the scores of mind-reading, memory span,working memory,processing speed,correlation coefficient was 0.227( P <0.05),-0.377( P <0.01),-0.334( P <0.01),-0.470( P <0.01),respectively. With mind-reading,cognitive processing and educational level as independent variables,with total scores, F1, F2, and F3 of TAS as dependent variable,respectively,the regression equation of total scores was significant( F =8.594,P ＜0.01), and the one of F1 was significant( F =8.796,P ＜0.01),but the one of F2( F =1.735,P ＞0.05) and F3( F =1.181,P ＞0.05) was not significant. Conclusion Elderly people's alexithymia is negatively related to mind-reading and several cognitive abilities. Mind-reading could affect emotional recognition, but not affect emotional description and export-oriented thinking.

Objective To assess the clinical diagnosis value of CT guided transthoracic core needle biopsy in patients with pathological changes.Methods The pathological diagnosis was performed after getting the tissue by the groovy needle punctured the lesion.Results The 165 patients included 153 focal pulmonary diseases and 12 diffuse pulmonary diseases,the defined pathological diagnostic rate was 92.1%,false negative rate was 3.9%,18.9%in malignant and benign pathological changes;and complication presented in 33.3%,pneumothorax,bleeding,hemoptysis and hypodermic haematoma were 17.0%,7.9%,7.3%and 1.2%,respectively.Conclusion The technique of pulmonary percutaneous biopsy under CT guided is a perfect method for qualitative diagnosis of difficult pulmonary pathological changes:it is of great clinical application.