Objective To investigate the antitumor activity of the filtrate of a novel Staphylococcus aureus with incompletehemolytic phenotype(SIHP), and to provide references for screening new antitumor drugs.MethodsThe epidemiologicalcharacteristics of the novel SIHP were summarized and analyzed. The hemolytic phenotypes of the novel SIHP, classic Staphy-lococcus aureus with complete hemolytic phenotype(SCHP) and quality control strains ATCC29213 and ATCC25923 weredetected on blood agar plate(BAP) by three-point method. Staphylococcus aureus filtrate was prepared by inoculating LBbroth, centrifuging and filtering after fermentation. The Staphylococcus aureus filtrate and its diluents with RPMI1640 ofdifferent volume fractions(1∶1, 1∶3, 1∶7) were co-incubated with human non-small cell lung cancer HCC827 cells, themorphological changes were observed, and the cells were classified and counted under a microscope.ResultsThe clinicalspecimen isolation rate of the novel SIHP was only 2. 01%, and its hemolytic phenotype on BAP was unique with strongrecognition. Compared with the classical SCHP and quality control strain ATCC25923, the novel SIHP filtrate showed signifi-cantly stronger tumor cytotoxicity, and damaged HCC827 cells in a concentration-dependent manner, leading to the release of cell contents, balloon-like degeneration of cell membrane or bare nucleus-like degeneration of cells.ConclusionThe novel SIHP may be a new subspecies of Staphylococcus aureus with a unique genetic background, and the filtrate contains highly effective antitumor active substances, which can provide a material basis for the screening of new antitumor drugs.

The clinical manifestation of endometrial uterine cancers is similar to other gynecological diseases like endometrial hyperplasia,polyps,uterine myoma,and adenomyosis.This similarity may result in missed diagnosis of endometrial cancer,which should be paid wide attention.In these cases,pathological examination reports malignancy of uterine specimen after simple hysterectomy.Personalized treatment,such as observation and reoperation,should be supplemented for these patients,according to the pathological characteristics of the tumor,risk factors of lymphatic and ovarian metastasis and the willingness of patient.

Objective To study CT and MRI value in diagnosis of osteoblastoma,in order to achive more information about the disease. Methods The clinical,CT,MRI and histological data of 12 patients with osteoblastomas. Results(1)There were 6 patients wereexamined by CT,the lesions showed mainly lytic with varying degrees of matrix mineralisation,and mild adjacent reactive sclerosis;(2)10 patients were examined by MRI(included 4 patients who have been examined by CT),the lesions presented mixed signal intensity with compart,slightly low and intermediate signal intensity on T_1-weighted images,high signal intensity on T_2-weighted images were found in 9 cases and mainly low signal intensity was found in 1 case.8 cases showed clear low signal intensity surrounding the lension borh on T_1 and T_2,2 cases showed blurry high signal in the adjacent tissue;(3) The tumors presented moderate enhancemen with low dense area inside the lesions after injection of contrast media. Conclusion Osteoblastomas have classical features on CT,while show no characteristic appearances on MRI.

Objective To study the conventional MRI features of adult Japanese encephalitis (JE)and explore the early features of diffusion weighted imaging(DWI).Methods Thirty-two patients with adult JE were included.MR scanning including T1 WI,T2 WI and FLAIR were done in all patients,and DWI in 28 patients.Twenty-three patients received DWI in the early stage (within 1 to 7 days),in which the appearances of DWI were analyzed,ADC graphs were obtained and the signal noise ratio and the ADC values of lesions were calculated.Results Twenty-eight cases were abnormal on MRI,and 4 patients were normal.The lesions of JE involved the thalamus in 22 cases,the substantia nigra of midbrain in 13,kellogg’s seahorse in 9,the basal ganglia in 7,the splenium corpo-ris callosi in 4,the pons in 6 and the insular lobe in 2,extensive cerebral edema in 2.The early features of DWI in 23 patients showed cytotoxic edema in 2,vasogenic edema in 12,both two edemas existing in 5,and normal in 3.DWI were superior to T2 WI in 6 cases,and T2 WI were superior to DWI in 9 cases,DWI was similar to T2 WI in 5 cases on showing the size and signal of the le-sions.Conclusion Adult JE often occurs in the thalamus,substantia nigra of midbrain and kellogg’s seahorse.DWI shows vasogenic edema in the early stage.Comprehensive analysis of DWI,ADC and T2 WI details helps to early detection and qualitative adult JE le-sions.

Objective: To demonstrate that expression of the CCRSDelta32 protein in PBMCs able to down-regulate surface expression of the HIV-1 coreceptor CCR5 and CXCR4.Methods:CCR5Delta32 gene was amplified from human peripheral blood mononuclear cells(PBMCs)genomic DNA by using PCR, and then cloned into lentiviral vector pLenti6/V5-D-TOPO.Recombinant lentiviral particles were produced by packaging using 293T cells.Human PBMCs were transfected with the constructed recombinant lentiviral particles and the expression of CCR5Delta32 was detected by Western blot.The level of CCR5 and CXCR4 expression on transfected PBMCs was detected by FACS analysis.Results: The recombinant lentiviral vector pLenti-CCR5Delta32 was constructed successfully, and the target protein was expressed in PBMCs.FACS analysis showed that CCR5Delta32 protein expressed in PBMCs was able to down-regulate cell surface expression of CCR5 and CXCR4.Conclusion: This study is expected to be used for the gene therapy on AIDS, which deserves further study.

Background and purpose:The incidence of Hashimoto’s disease (HD) coexistent with thyroid carcinoma is 0.5%-38%, and it is increased significantly in recent years. Therefore, we investigated the clinical characteristics and surgical experiences in patients with HD coexistent with thyroid neoplasm. Methods:The clinical data in 417 cases of HD coexistent with thyroid nodules including 93 cases of HD coexistent with thyroid carcinoma treated surgically and confirmed pathologically from Jun. 1985 to Dec. 2007 were analyzed. Results:The coexistent rate of HD with thyroid carcinoma (TC) was 22.3% respectively. The coexistent rate of HD with TC was 9.2% before 1995, and it went up to 24.7% after that year (P

Background and purpose: The incidence of Hashimoto's disease (HD) coexistent with thyroid carcinoma is 0.5%-38%, and it is increased significantly in recent years. Therefore, we investigated the clinical characteristics and surgical experiences in patients with HD coexistent with thyroid neoplasm. Methods: The clinical data in 417 cases of HD coexistent with thyroid nodules including 93 cases of HD coexistent with thyroid carcinoma treated surgically and confirmed pathologically from Jun. 1985 to Dec. 2007 were analyzed. Results: The coexistent rate of HD with thyroid carcinoma (TC) was 22.3% respectively. The coexistent rate of HD with TC was 9.2% before 1995, and it went up to 24.7% after that year (P<0.05). Among those HD coexistent with TC patients, 66 cases were papillary carcinoma, 15 cases were follicular carcinoma, 9 cases were mixture and 3 cases were lymphoma. There were 43 cases of micro-foci thyroid carcinoma, which accounted for 46.2% of the malignancy. Unilateral or bilateral, total, subtotal or partial thyroidectomy and biopsy were the main operative procedures for the disease. Conclusion: There is a high incidence of thyroid carcinoma in patients with HD coexistent with thyroid nodules, and it is rising up recently. In the clinical treatment of HD, we should be aware of the coexistent thyroid tumors and micro-foci thyroid carcinoma.

Objective To investigate the effects of environmental endocrine disruptors-phytoestrogen soybean isoflavone on serum glucose and lipid in rats with orchiectomy.Methods Fifty-four healthy clean male Wistar rats aged 7 weeks were randomly divided into 6 groups,9 in each group,according to their weight and named as the groups of SHAM,ORCH,L-SI,M-SI,H-SI and EC.The rats were injected intraperitoneally with 1.5% sodium pentobarbital according to 40 mg/kg,and they were either shamoperated or orchiectomized.The rats were fed on high fat diet for 4 weeks.There was 1,3,9 g soybean isoflavone and 0.45 mg estrobene per kilogram high fat diet in L-SI,M-SI,H-SI and EC respectively.At the 2nd and 4th week,serum insulin,fasting blood glucose and lipid (TG,TC,HDL and LDL) were detected.Results The weights of rats with orchiectomy was significantly decreased compared with sham-operated rats,and compared with the EC group,the weights of other groups'rats were significantly higher.The weight was decreased along with the increase of the dose of soy isoflavone.At the end of 4 weeks,SIF significantly decreased serum glucose compared with ORCH,and the insulin level was significantly increased in M-SI and H-SI group.SIF and estrogen could significantly decrease TC,but they did not have obvious effect on LDL.SIF could not improve HDL level in rats with orchiectomy.Even though there was no statistic difference,the TG levels of SIF groups were deceased.Conclusion SIF can decrease serum glucose level via raising insulin level in rats with orchiectomy.SIF can decrease serum TC level significantly and regulate TG level while there is no influence on HDL and LDL.

In order to evaluate the effect of chinese medicine (Yi Qi Xiao Zheng decoction) in reducing radiation side effect, an observation of peripheral blood WBC, stem cell amount, the function of liver and renal in mice was made during and after radiation.The results showed that WBC and stem cell amount were higher in Chinese medicine combined with radiation group than radiation only group after 3 fraction radiation(P

Objective To explore the feasibility for applying passive individual samplers to determine NO2 and SO2 in the air simultaneously and to monitor the levels of them in the indoor and outdoor air. Methods Ten households were selected in urban and suburban area of Beijing respectively. Passive individual sampler made by Green blue Co. in Japan was used to sample and the ion chromatographic technique was used to determine the concentration of NO2 and SO2. Results There was no significant difference had been seen between the NO2 and SO2 level monitored by passive sampler and by the assay recommended by the national standard. It showed that the levels of NO2 and SO2 in ambient air were higher that that in indoor air, both indoor and outdoor air pollution in the urban area was more serious than that in the suburban area. The outdoor sulfur dioxide concentration in the urban area in winter was as 3.14 times high as that in the suburban area in 2001. The level of sulfur dioxide in the urban ambient air in 2002 was much lower than that in 2001. Conclusion Passive individual samplers with molecular diffusion will be suitable for environmental quantity monitoring.