Objective:To study the biologic characters of human foreskin fibroblasts(HFF)to lay the foundation for a long-term culture for human embryonic germ cells in vitro.Methods:The method of separating HFF was investigated.By immunocytochemical method,MTT and flow cytometry,we investigated the morphology and the purity of HFF,study the cell growth curve and cell cycle of HFF,and the effects of different concentrations of mitomycin on growth of HFF at different times.Results:Epidermis and dermis were more easily separated after overnight digestion(12~16 h)in 0.25% Trypsin at 4℃,compared with incubation for 2 h in 5% CO2 at 37℃.Vimentin was strongly positive stained with the purity of 95 percent.There was no obvious stranded period.The rapid proliferation period was in 1~6 d of cell growth curve.The platform stage began at 7 d.Majority of the cells were in G1/G0 phases.The cell cycle corresponded to cell growth curve.Mitomycin C(MMC)inhibited the proliferation of HFF at 12.5 ?g/ml over 2.5 h of MMC treatment.Conclusion:Its biological characters showed that HFF can be used as feeder layer for in-vitro cultured human embryonic germ cells。

Analysis of differences between traditional Chinese medicine (TCM) philosophy with malnstream western medicine was done with reference to intercultural communication theory. It was found that the differences are malnly manifested through holism philosophy of TCM and dualism of Western medicine, intuition thinking mode of TCM and mechanical thinking mode of western medicine, and ideographic Chinese language and objective description of the Western medicine. The translation principle was also discussed with the perspective of intercultural communication. It is of great significance to respect the recipient’s privilege, to create intimate text, and to understand the bilingual differences if TCM translation is to be promoted.

Target volume definition is one of the important factors in precise radiotherapy of esophageal carcinoma.However,there is considerable controversy about delineation of esophageal carcinoma clinical target volume (CTV),and no consistent standard.Recent researches show that pathological characteristics,imaging methods and lymph node metastasis pattern may have guiding functions for CTV delineation.Novel delineation technique such as individual design has been a research hotspot.

Objective To establish an analytical method for fingerprint of Citri Reticulatae decoction pieces by HPLC. Methods Thermo C18 chromatographic column (4.6 mm×250 mm, 5μm) was used with methanol-0.3%phosphoric acid in gradient elution. The flow rate was 0.8 mL/min, the detection wavelength was set at 280 nm, and the temperature was 40 ℃. The similarities of the 15 batches of samples were compared by similarity evaluation, cluster analysis and principal component analysis. Results Based on the fingerprints of Citri Reticulatae decoction pieces, 8 common peaks were identified. The similarities were all more than 0.9 among all batches. 15 batches can be divided into three categories. Conclusion The method is simple and steady. It provides a basis for the quality control and evaluation of Citri Reticulatae decoction pieces.

The human sclera accounts for 95％ of the surface of the eyeball,providing ample contact area which is suitable for targeted trans-scleral ocular drug delivery.Currently there are several tans-scleral sustained-release strategies,including intra-scleral delivery,episcleral delivery,as well as tans-scleral iontophoresis.Different devices and methods have their own advantages and disadvantages,for example,intrascleral delivery is somehow invasive,and episcleral delivery device needs to be made thin to prevent erosion of conjunctiva,iontophoresis needs to be frequently repeated as of its short-term effect.With the development of bio-material engineering technology,episcleral microfilm could become an ideal drug delivery route for posterior segment ocular diseases.

The application of evidence-based medicine in bibliotherapy is defined as evidence-based bibliothera-py.After a description of the connotation, role and significance of bibliotherapy, and foreign and domestic studies on it, the problems in domestic bibliotherapy were pointed out with measures put forward for their solution, and the implementation of bibliotherapy was normalized in view of its methodology.

Lung cancer is one of the most common malignant tumors in the world .However,its pathogen-esis is still unclear .With the deep research of related signal pathways underlying the development ,progression and prognosis of lung cancer , we find that the mTOR signaling pathway , JAK-STAT pathway , Notch signaling and IGF have been identified to play a major role in the growth ,proliferation,differentiation,apoptosis and invasion of tumor cells.Therefore this paper is to review the effects of the above several pathways ,which may prove a new ba-sis for the pathogenesis and therapy of lung cancer .

Objective:To make full use of existing variables set,study on the dynamic connection between economic growth,health input and investment benefits.Methods:Using Factor Augmented VAR(FAVAR) to draw common factors and perform VAR estimation with the variables sets.Results:The impact of economic growth on health output had two sides.Increasing health input would significantly stimulate health output and economic growth.The increase of health level would promote economic growth in short time and hamper in the long run.Conclusion:The government should increase health input and consider the health loss brought by economic growth.It should prevent the not rich but old risk with the pursuit of health benefit.

Objective To study the effective constituents from the stem bark of Erythrina variegata.Methods The compounds Ⅰ-Ⅸ were isolated by a combination of silica gel,Sephadex LH-20,and ODS chromatographies and their structures were identified by spectral methods.Results Nine known compounds were isolated from the stem bark of E.variegata.Their structures were identified by means of spectroscopic analysis as: 5,4′-dihydroxy-8-(3,3-dimethylally)-2″,2″-dimethylpyrano [5,6∶6,7] isoflavone(Ⅰ),stigmasterol(Ⅱ), erythrinasinate B(Ⅲ),3-hydroxy-2′,2′-dimethylpyrano [5,6∶9,10] pterocarpan(Ⅳ),5,7-dihydroxy-8-(3,3-dimethylally)-dihydroflavanone (Ⅴ),5,4′-dihydroxy-2″,2″-dimethylpyrano [5,6∶6,7] isoflavone(Ⅵ),5,7,4′-trihydroxy-6,8-di(3,3-dimethylally) isoflavone(Ⅶ),5,2′,4′-trihydroxy-8-(3,3-dimethylally)-2″,2″-dimethylpyrano [5,6∶6,7] isoflavone(Ⅷ),5,4′-dihydroxy-6-(3,3-dimethylally)-2″,2″-dimethylpyrano [5,6∶7,8] isoflavone(Ⅸ).Conclusion Compounds Ⅰ,Ⅲ,Ⅳ,Ⅶ,and Ⅸ are isolated from this plant for the first time and compound Ⅴ is newly isolated from the plant of Erythrina Linn.