OBJECTIVE:To observe the clinical therapeutic effect of KLT combined with chemotherapy on late can?cers.METHODS:64patients with late cancers were assigned as observation group:receiving KLT+chemotherapy;47patients with late cancers were assigned as control group:receiving routine chemotherapy.RESULTS:In the observation group,the ef?fective rate was48.44%,body weight increase accounted for57.81%,KSP increased85.93%and relief from pain accounted for71.87%;In the control group:the effective rate was34.04%,body weight increase accounted for22.66%,KSD increased48.93%and relief from pain accounted for46.81%.The incidence of ARDs in observation group was obviouly lower than those in control group.CONCLUSION:KLT combined with chemotherapy can raise the therapeut effect on patients with late cancers and improve their life quality.

Gait recognition is a new technology in biometric recognition and medical treatment which has advantages such as long-distance and non-invasiveness. Depending on the differences between different people's walking pos tures, we can recognize individuals by characteristics extracted from the images of walking movement. A complete gait recognition process usually includes gait sequence acquisition, gait detection, feature extracting and recognition. In this paper, the commonly used methods of these four processes are introduced, and feature extraction methods are classified from different multi-angle views. And then the new algorithm of multi-view emerged in recent years is highlighted. In addition, this paper summarizes the existing difficulties of gait recognition, and looks into the future development trends of it.
Algorithms ; Biometry ; Gait ; Humans ; Image Interpretation, Computer-Assisted ; Walking

[ ABSTRACT] Diabetes is characterized by an absolute or relative deficiency inβ-cell mass, which cannot be re-versed with existing therapeutic strategies.The restoration of the endogenous islet β-cells can stabilize the level of blood glucose.The isletβ-cells can be obtained from the directional differentiation of stem cells, but the process is complex and has the risk of teratomas generation.Cell direct reprogramming, one terminal differentiated cell can transdifferentiate into another kind of terminal differentiated cell, which is other than directional differentiation from stem cells.Direct reprogram-ming gives rise to the generation of isletβ-cells from one terminal differentiated cell, may be preferable for diabetes therapy because of its unique advantage.

Objective To investigate the value of serum ischemia-modified protein (IMA),hypersensitivity C reaction protein (hs-CRP),myoglobin (MYO)creatine kinase MB (CK-MB)and ultra-sensitivity troponin T (hs-cTnT)in the early diagnosis of patients with acute coronary syndrome(ACS).Methods Serum levels of IMA,hs-CRP,MYO,CK-MB and hs-cTnT were meaused in 94 patients with ACS[including 40 cases of unstable angina pectoris(UAP),20 cases of non-ST segment elevation myocardial in-farction(NSTEMI),non-Q wave myocardial infarction and 34 cases of ST segment elevation myocardial infarction(STEMI),Q wave myocardial infarction]and 99 cases of controls.The efficiency,sensitivity,specificity,negative and positive predictive values in the early diagnosis of ACS were compared among 5 kinds markers by the ROC curve.Results The detection results of serum IMA,hs-CRP,MYO,CK-MB and hs-cTnT had statistically significant differences between the UAP,NSTEMI and STEMI groups with the normal control group (P <0.05),serum IMA,hs-CRP,MYO,CK-MB and hs-cTnT had statistically significant differences between the UAP group with the NSTEMI group and between the UAP group with STEMI group (P <0.05),but the difference between NSTEMI and STEMI group group was not statistically significant (P >0.05).Conclusion In the patients with ACS,the multiple indicators combined detection can achieve the early diagnostic value.

BACKGROUND: Artesunate can relieve pulmonary fibrosis, but its mechanisms are rarely reported.OBJECTIVE: To explore the effect of artesunate on apoptosis of HFL-I cells and the role of Fas, FasL and Caspase-3 in the artesunate-mediated apoptosis of HFL-I cells. METHODS: Cell counting kit-8 (CCK-8) assay was used to determine the effects of artesunate at 1, 10, 100 mg/L on the growth of HFL-I cells in vitro. Apoptosis ratio was examined by flow cytometry (FCM). The mRNA level of Fas, FasL and Caspase-3, were assessed by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS AND CONCLUSION: Artesunate had a significantly inhibitory effect on the proliferation of HFL-I cells in a dose-dependent manner in vitro. The apoptosis rate of HFL-I cells was significantly increased in the artesunate-treated group compared with the control group (P < 0.05 or P < 0.01). The mRNA levels of Fas, FasL and Caspase-3 were significantly higher in the artesunate-treated group than the control group (P < 0.05). The findings of this study demonstrate that artesunate can exert a marked anti-pulmonary fibrosis effect by up-regulating mRNA levels of Fas, FasL and Caspase-3, which can induce the growth inhibition and apoptosis in HFL-I cells.

Objective To detect the serum levels of epidermal growth factor（EGF）in rheumatoid arthritis（RA）patients,and explore the possible role of EGF in RA.Methods The serum level of EGF was measured by enzyme-linked immunosorbent assay（ELISA）in 38 RA patients diagnosed according to dianostic criteria for RA by American Rheumatism Association and 20 healthy controls.Rheumatoid factor（RF）,erythrocyte sedimentation rate（ESR）and C-creative protein（CRP）were also measured in these 38 RA patients.The relation beween the serum level changes of EGF and the corresponding clinical indexes in the two groups was statistically analyzed.Results Compared with the healthy individuals,the serum EGF levels of RA patients increased significantly（P 0.05）.Compared with simple bone rarefaction patients,the serum levels of EGF increased significantly in the bone erosion patients（P 0.05）.The serum levels of EGF in RA patients had no correlation with RF,ESR and CRP levels（P 0.05）.Conclusion The evident increase of serum EGF level in RA patients,especially in bone erosion patients,suggests that EGF may partly play a role in the pathogenesis of RA.

Objective Summarize the lesion characters, procedure results and clinical follow up results of the elderly patients with coronary heart disease who underwent coronary angioplasty Methods 53 cases of coronary heart disease patients undergoing PTCA and stenting were ≥65 years old 35 were male, 18 were female 33 patients were diagnosed acute Q wave myocardial infarction, 2 patients acute non Q wave myocardial infarction, 14 patients unstable angina pectoris, and 4 patients coronary heart disease with severe heart failure PTCA and stenting were performed with conventional method, and temporary pacemakers were used if necessary Heparin was used if target vessel were involved in small vessels or the patients had multivessel diffuse lesions or PTCA was performed in more than one vessel Results Multivessel diffuse severe atherosclerosis was common in elderly patients The lesions were complex, and the procedures were difficult The success rate was 98% and the mortality was 11% during hospitalization 30 patients were follow up in 8 7?5 4 months, during which 11 (36%) patients had angina, 1 (3 5%) patients suffered acute myocardium infarction, 1 (3 5%) patient died of cerebral infarction, and the other 17 patients were asymptomatic Conclusion PTCA for the elderly patients is feasible The lesions in elderly patients with coronary heart disease are complex, the procedures are difficult and the mortality in hospitalization and the recurrent of ischemic chest pain are higher However, if we sufficiently estimate the lesion′s characters, PTCA can be safely performed in the elderly patients, and PTCA is the better method for elderly patients than medicine treatment

Objective:To explore the therapeutic effect of the extraction of bone-melon injection for the earlier fracture.Method:100 patients with early fracture were divided into two groups.50 cases in the treatment group used the extraction of the bone-melon injection and 50 cases in the controlled group used compound ossotide injections.Their curative effects were judged according to their healing time,level of relieving pain and functional recovery condition.Result: The patients in the treatment group healed earlier(P

BACKGROUND:Excessive mechanical stimulation can lead to endoplasmic reticulum stress. Endoplasmic reticulum stress plays an important role in the occurrence and development of degenerative diseases. Caspase-12 is a specific molecule of endoplasmic reticulum stress, and the intensity of endoplasmic reticulum stress can be reflected by caspase-12 expression. OBJECTIVE:To observe the expression rule of Caspase-12 in chondrocytes under the cyclic tensile stress. METHODS:Human chondrocytes were used as test subjects. After group assignment, mechanical loading system was used. The loading group and corresponding Z-ATAD-FMK inhibitor group received 2, 12, 24, 36, and 48 hours of mechanical stimulation. The blank group (0 hour) and the paired Z-ATAD-FMK inhibitor group received the same process as the loading group except the loading. After loading, cel morphology and growth were observed under the microscope. RT-PCR and western blot assay were used to detect the expression changes of caspase-12 gene and protein in each group. RESULTS AND CONCLUSION:(1) Morphological observation results demonstrated that apoptosis appeared at 2 hours after loading, peaked at 24 hours. With prolonged time, cel growth showed the trend along stress, but apoptosis weakened. It is indicated that cyclic tensile stress could make chondrocyte apoptosis. Endoplasmic reticulum stress might be activated. The model of cel s in vitro was established successful y. (2) Caspase-12 gene and protein expression showed consistent trend, and peaked at 24 hours, which showed significant differences as compared with the blank and inhibitor groups (P<0.05). (3) Cyclic tensile stress can induce chondrocyte endoplasmic reticulum stress, and affect caspase-12 expression.

Objective To explore the value of spectral curve in the differential diagnosis of benign and malignant tumor of the musculoskeletal system.Methods 100 patients with mass of the musculoskeletal system which were found by MRI underwent Dis-covery HD750 Gemstone CT scan ,and the images acquired were processed.Areas with homogenous density which did not contain necrosis,cyst,hemorrhage and calcification were chosen as region of interest(ROI).The optimal Kev on single energy images was selected,then the Kev-CT curve was automatically generated.All 100 patients proved by histological examination were divided into four groups,38 cases were as group A with benign tumor,10 cases as group B with borderline tumor,49 cases as group C with ma-lignant tumor and 3 cases of lipoma(that were analyzed separately since its curve was arc shaped).Average slope rate of every group was calculated according to the formula:K=(HU40keV-HU80keV)/40.Kruskal Wallis test was applied for statistical analysis,P value of less than 0.05 was considered statistical significant.Results The spectral curve of group C was sharply falling type with a mean slope rate of 1.64±1.00,that of group A was gradually falling type with a mean slope rate of 0.75±0.30,and that of group B was 1.34±0.45.The difference of slope rate between group A and C and between A and B were of statistical significance(P<0.05).The spectral curve of 3 lipomas showed arc shaped rising type with a mean slope rate of-2.00.Conclusion Spectral curve is useful in the differential diagnosis of benign and malignant tumor of the musculoskeletal system.Arc shaped curve is a specific sign for tumors containing abundant fat.