Objective To analyze the change trend of drug resistance in clinical strains of Escherichia coli,and to provide the basis for rational use of antibiotics in clinic,and to increase the level of drug resistance.Methods The drug resistance of Escherichia coli isolated from various clinical specimens in hospital from January 2012 to November 2016 were analyzed retrospectively.Results From all kinds of clinical specimens 3 458 strains of Escherichia coli were isolated,extended spectrum beta-lactamases producing rate of which was 57.92％.The drug resistance rates of Escherichia coli to Quinolones,Penicillins,Cephalosporins,Aminoglycosides,Sulfonamides were more than 50.00％;The resistance rate of Imipenem,Meropenem,Piperacillin/Tazobactam,Cefoperazone/Sulbactam and Cefoxitin were less than 10.00％ of the antibacterial drugs,the resistance to some third,forth generation Cephalosporins were significantly increased.The Wilcoxon test showed that drug resistance rate of Escherichia coli isolated from sputum was significantly higher than that of blood,urine,secretions,the difference was statistically significant(P<0.05).Conclusion Clinicians should attach great importance to which the drug resistance of Escherichia coli in a hospital increased year by year.

AIM: To investigate the anti-apoptotic effect of hepatocyte growth factor(HGF) on actinomycin D(ActD)-induced apoptosis in hepatocytes and the possible pathway.METHODS: Hepatocytes were exposed to ActD and HGF.The cytotoxic effects of ActD were tested by MTT.Apoptotic cells were identified by Hoechst 33342 staining,flow cytometry and detection of DNA fragmentation with agarose gel.Akt and phospho-Akt were detected by Western blotting analysis.RESULTS: The results showed that ActD induced apoptosis in hepatocytes 5 h after treatment.Phosphatidylinositol-3 kinase(PI-3K) specific inhibitor wortmannin enhanced the apoptotic effect of ActD.Furthermore, HGF significantly reduced the apoptosis in hepatocytes induced by ActD in a concentration-dependent manner.In the presence of wortmannin,HGF did not overcome apoptosis.CONCLUSION: Wortmannin enhances the apoptotic effect of ActD.HGF protects hepatocytes from apoptosis induced by ActD through a PI3K/Akt pathway.

ObjectiveTo discuss neonatal hypoxic-ischemie eneephalopathy points,and the vesting of the re- lationship between the prognosis and current treatment methods. MethodsFrom January 2004 to April 2008, pedi-atric hospital treated 88 cases of HIE were assessed, inspected, grading and treated. Results8 cases in the 88 easesantomatically discharged, 51 patients (63.8 %) were recovered and 28 patients (35.0 %) improved, and 1 patient(1.3 %) died. HIE clinical severity has close relationship with prognosis. Mild and moderate cases were 78.4 % (29/37) ,53.7% (22/41) were recovered and no deaths, and severe 1 case, 1 death. ConchtsionHIE clinical severityand prognosis have close relationship, the time of birth asphyxia situation on the vesting of infants discharged andwas no significant impact.

Laser has been used in the treatment of glaucoma for more than twenty years.Its main applications include laser peripheral iridoplasty,laser trabeculoplasty,laser sclerostomy,and laser cyclophotocoagulation. Other applications such as C02 laser filtering surgery and photodynamic therapy(PDT)have just started.In this paper,a review is provided on the present applications of lasers in the treatment of glaucoma including choice of laser,indications,surgery approach and efficacy.The prospect of the future application is also discussed.

Objective To evlauate the effect of pulmicort respule combined with solution salbutamoI aerosol on the treatment of bronehiolitis.Methods 65 cases of bronchioiltis were randomly divided into two groups.solution saltutamol inhalation group or pulmicort respule combined with solution salbutamoI group,the time of easing asthma symtpoms was assessed.Results Therapeutic group cure rate is higher than the control group(87.9%/62.5%,P＜0.01),there is significant difference.Observation group duration of symptoms was significantly shorter than the oontrol group(P＜0.01),the difference is significant.Condmlon Pulmicort respule combined with solution salbutamol has significant therapeutic effect on the treatment of bronchiolitks,there are obvious advantages.

Objective To discuss the clinical manifestations and the morphological features of intracranial arteriovenous malformation (AVM) in childhood. Methods During the period from Jan. 2012 to June 2013, a total of 14 AVM patients under the age of 14 years were admitted to authors’ hospital. Of the 14 patients, 11 had a history of spontaneous intracerebral hemorrhage (78.6%), 7 complained of headache (50.5% ), 5 showed signs of unilateral limb weakness (35.7% ), and 2 was accompanied with epileptic attacks (14.3% ). The clinical characteristics, the preoperative imaging features and DSA findings were retrospectively analyzed. Results DSA showed that terminal feeding branches were visualized in 9 cases and blood supply from perforating branches was seen in the remaining 5 cases. In seven cases the feeding artery was single, while in the remaining 7 cases the feeding artery was multiple, and in four of them combination blood supply from both anterior and posterior circulation was found. The lesions were situated at the lateral or convex surface of cerebral hemisphere (n = 5), lateral ventricle (n = 4), cerebral bottom (n = 3) or medial surface of cerebral hemisphere (n = 2). The lesion’s diameter was < 30 mm in 10 cases and (30 -60) mm in 4 cases. According to Spetzler- Martin grading, grade Ⅰ was seen in 4 cases, grade Ⅱ in 2 cases, grade Ⅲ in 5 cases and grade Ⅳ in 3 cases. Conclusion Although in childhood AVM lesion is small in size and lower in grading, its onset is early and the lesion is prone to bleeding. DSA findings indicate that most AVMs in childhood take the form of immature type. Nevertheless, its natural course needs to be further studied.

Objective To isolate and purify polysaccharide from Angelica sinensis by ultrafiltration technology. Methods Extract of polysaccharide of Angelica sinensis was ultrafiltrated with different aperture membrane, the separation parameters on membrane separation efficiency of polysaccharide of Angelica sinensis were optimized by and orthogonal test. Results Polysaccharide of Angelica sinensis was separated with 200, 100, 50, 20 kD membrane. The contents of polysaccharide was 30.47% before ultrafiltration. The main component of Angelica sinensis polysaccharide was the fraction with 200 kD (65.42%). The optimum condition of ultrafiltration was obtained as follow:one times of extract volume, 35 ℃, 0.3 MPa. Conclusion The technology was simple and available, and the content of Angelica sinensis polysaccharide was higher.

Objective To know the blood lead level among twins in Ji’ning,Shandong province,China and to assess the genetic and environmental influence on blood lead level of children. Methods A total of 144 pairs of same sex twins aged 6-14 years were randomly selected in June,2008,and their blood lead level was determined and assessed into three groups,namely the relatively safe group (blood lead level

The aim of the study was to explore the role of NF ?B activation in the pathogenesis of smoke inhalation injury and to evaluate the effects of dexamethasone on NF ?B activation. 130 Wistar rats were inflicted with smoke inhalation injury and randomized equally into the control group, smoke inhalation injury group and dexamethasone treatment group.The expressions of NF ?B P 65 ,I?B ?,TNF ?,and ICAM 1 proteins in the lung tissue were determined by immunohistochemistry at 2,6,12,24,48,72 hours after smoke inhalation injury. The results showed that,after smoke inhalation ,the expressions of NF ?BP 65 ,TNF ?,and ICAM 1 increased,whereas the expression of I?B ? decreased. In the dexamethasone treatment group,the expressions of NF ?BP 65 ,TNF ?,and ICAM 1 were down regulated, and I?B ? was up regulated.These results suggest that NF ?B activation may participate in the pathogenesis of smoke inhalation injury.Dexamethasone could suppress NF ?B activation, thus partially blocked the production of cytokines and adhesion molecules,and in turn reduced the damage of inflammatory response. Therefore NF ?B activation might be a key point in the development of smoke inhalation injury and modulation of activation of NF?B might be a potential therapeutic strategy to treat this injury at the transcription level.