This paper discusses the actuality of the artificial bioactive functionally gradient material and its developing trend.

Objective To investigate the changes and clinical indications of interleukin-2 receptor, ferritin and fi-brinectin in the cerebrospinal fluid of central nervous system leukemia.Methods ELISA was used to detect the changes of-soluble interleukin-2 receptor, ferritin and fibrinectin in the cerebrospinal fluid of central nervous system leukemia group ( CNSL) and non-CNSL group.Results The concentrations of soluble interleukin-2 receptor, ferritin and fibronectin ince-rebrospinal fluid of the CNSL group were significantly higher than those in non -CNSL group and the control group ( p <0.05).Conclusion The soluble interleukin 2 receptor, ferritin and fibronectin in cerebrospinal fluid are indicative of cen-tral nervous system leukemia.The result suggests that combined detection is important in early diagnosis of central nervous system leukemia.

Objective:To investigate the mutation of Brg1 gene and expression of Brg1 protein in gastric adenocarcinoma,and to study its relation with tumor development.Methods:By means of polymerase chain reaction-single strand conform action polymorphism(PCR-SSCP)37 cases of gastric mucosa were searched for Brg1 gene mutation in exon 4 and 16.By immunohistochemistry the expression of Brg1 protein in 30 cases of gastric adenocarcinoma was analyzed for their role in gastric cancer development and prognosis.Results:In 37 cases of gastric adenocarcinoma,no mutation of Brg1 gene was detected.Brg1 protein expression was 76.67% in gastric carcinoma which was higher than that in normal tissue(44.44%.) The expression of Brg1 was related to tumor growth,lymphnode metastasis and tumor differentiation.Conclusion:Genetic abnormalities of Brg1 might be rare and might not play a major role in stomach carcinogeneses.An increased expression of Brg1 protein was associated with the advanced stage of gastric carcinoma,the Brg1 expression can be used as an important indicator for differentiating malignancy and prognosis of gastric carcinoma.

To compare the efficacy and adverse effects of bortezomib+cyclophosphamide+dexamethasone (PCD) and vincristine+adriamycin+dexamethasone (VAD) regimens in multiple myeloma (MM). Methods:A total of 41 patients with MM were analyzed retrospectively and divided into two groups according to their treatment protocols:PCD group (21 cases) and VAD group (20 cases). Clinical effects and adverse effects were observed in both groups. Patients accepted two to four cycles of PCD or VAD regi-mens. Results:In the PCD group, three patients achieved complete remission (CR), three patients had very good partial remission (VG-PR), three patients were under partial remission (PR), eight patients had stable disease status (SD), and four patients had progressive dis-ease (PD). In the VAD group, none achieved CR, one patient had VGPR, two patients were under PR, nine patients had SD status, and nine patients had PD. The rate of patients who achieved efficacy (CR+VGPR+PR) in the PCD group was 42.9%, which was higher than that of the VAD group (15.0%). The rate of newly diagnosed patients who achieved good efficacy (CR+VGPR) in the PCD group was 50%, which was higher than that of the VAD group (7.7%). The incidence of herpes infection, cytopenia, fatigue, and gastrointestinal symptoms was similar in the two groups, whereas the incidence of neurotoxicity in the PCD group was higher than that of the VAD group. Conclusion:The response rate of PCD is higher compared with that of conventional VAD chemotherapy, especially in newly di-agnosed MM. PCD may improve CR and VGPR rates and may bring about more severe toxicities, such as neuropathy.

Objective To compare the results difference between the fluorescence staining and the acid fast staining (Ziehi‐Neelsen staining) methods in the detection of Mycobacterium tuberculosis ,and to compare the effects of the methylene blue solu‐tion ,Haris hematoxylin solution and potassium permanganate liquid as the redyeing reagents of the fluorescence staining method . Methods 198 sputum specimens collected from the patients with suspected tuberculosis symptoms and were performed the Ziehi‐Neelsen staining and the fluorescence staining respectively For comparing the difference in the detecting rate of Mycobacterium tu‐berculosis between the two kinds of method .The fluorescence staining adopted 0 .3% methylene blue solution ,0 .5% Haris hema‐toxylin solution and 0 .5% potassium permanganate solution as the redyeing reagents for comparing the effects of the fluorescence microscopic examination among different redying reagents .Results The detection rate of Mycobacterium tuberculosis was 66 .67%(132/198) for the Ziehi‐Neelsen staining ,94 .9% (188/198) for the fluorescence stainings and 94 .95% (188/198) for the methyl‐ene blue staining ,in which the detection rate of methylene blue redyeing was 94 .95% ,which of hematoxylin redyeing was 94 .44%(187/198) and which of potassium permanganate redyeing was 94 .44 (187/198) ,the differences among them were statistically sig‐nificant(P< 0 .05) .Conclusion The fluorescent staining method has the higher positive detection rate of Mycobacterium tubercu‐losis than the Ziehl‐Neelsen staining method ,in which 0 .3% methylene blue solution is a good background quenching redyeing solu‐tion .

This paper summarized the application of Female Sexual Function Index (FSFI), and introduced its scoring system.

The fatty acid components and microviscosity of erythrocyte membrane in 39 type 2 diabetic patients with and without coronary heart disease(CHD) were examined by high-performance liquid chromatography (HPLC) and fluorescence polarization teachnique,and their relationship with insulin sensitivity index (ISI) and CHD were analyzed.The results showed that contents and composition of erythrocyte membrane arachidonic acid (AA,C20:4)were significantly lower in type 2 diabetic patients with or without CHD than those in control subjects (P＜0.01 and P＜0.05).The total fatty acids contents were also significantly lower in patients with CHD than those in normal subjects (P＜0.05).In patients with or without CHD,erythrocyte membrane microviscosity was significantly increased as compared with the controls (P＜0.01 and P＜0.05),and it was higher in diabetics with CHD than those without CHD (P＜0.05).AA contents of erythrocyte membrane was negatively correlated with microviscosity and positively correlated with insulin sensitivity index (ISI),while the microviscosities were negatively correlated with ISI in diabetics.AA,linoleic acid contents,microviscosity of erythrocyte membrane and ISI were all correlated with the incidence of CHD in diabetics.In diabetic patients,the fatty acid metabolic abnormality could cause the alterations of functions,structures and fluidity of erythrocyte membrane,thus may contributing to increased IRS and might be related to the incidence of CHD.

Objective To study the phosphatidylinositol 3-kinase (PI3K) and protein kinase B (Akt) expression and clinical significance in the tissue of epithelial ovarian cancer (EOC).Methods Western Blot and immunohistochemical staining were applied to investigate the expression of PI3K and Akt in specimens of 41 patients with EOC (EOC group),20 patients with normal ovary (NO,NO group) and 20 patients with benign epithelial ovarian tumor (BEOT,BEOT group).Results The positive expression rate of PI3K in EOC group [70.7％(29/41)] was significantly higher than that in NO group and BEOT group [10.0％ (2/20),20.0％ (4/20)] (P ＜0.01).The positive expression rate of Akt in EOC group [73.2％ (30/41)] was significantly higher than that in NO group and BEOT group [10.0％ (2/20),30.0％ (6/20)] (P＜ 0.01).There was significant difference in the expression of PI3K and Akt between Ⅰ-1Ⅱ stage and Ⅲ-Ⅳ stage in EOC patients (P ＜ 0.05).There was no significant difference in the expression of PI3K and Akt between serous cystadenocarcinoma and mucous cystadenocarcinoma (P ＞ 0.05).Conclusions The expression of PI3K and Akt increases obvioualy in the tissue of EOC.PI3K and Akt may play important roles in carcinogenesis and metastasis of EOC.PI3K/Akt pathway may be served as a potential target for anticancer therapy.

Objective To explore the curative effect of dynamic hip screw(DHS) and proximal femoral nail (PFN)in treatment of intertrochanteric fractures. Methods Dynamic hip screw(DHS) group is A group:30 patients, proximal femoral nail( PFN) group is B group:30 patients. In the respect of the average length of the incision, operation time,blood loss,X-ray exposure times,postoperative drainage volume,blood transfusion volume,antibiotics usage time,hospitalization time,postoperative ambulation time,healing time of fracture,criteria of Harris hip function score after half a year,complication rate,this paper made the comparative analysis of clinical efficacy and statistical analysis between the two groups. Results 60 patients were followed up for 9 months to 4 years, with the average of 2 years and 3 months and no death. The average length of the incision, operation time, blood loss, X-ray exposure times, postoperative drainage volume,blood transfusion volume, DHS group were( 13. 4 ± 1. 9) cm, (92. 8 ± 13. 0) min, (321.0±39.4)ml,(5. 8 ± 1.2)times, (84.1 ± 10. 1)ml, (316.6 ±79. 1 )ml;PFN group were(7.6 ± 1.1 )cm,(59. 0±6. 9)min, (204. 6 ±32. 7) ml, (5.0 ±0.9) times, (53. 9 ± 8. 7) ml, ( 196. 6 ± 18. 2) ml; the differences between two groups were statistically significant ( all P ＜ 0. 05 ) . Hospitalization time, postoperative ambulation time, healing time of fracture,DHS group were(6.2±3.7)d,(17.8±1.8)d,(69. 8 ± 12. 3)d, (13. 0 ± 1. 6) weeks,PFN group were(4.7 ±2. 6)d,( 16.0 ±2. 2)d, (46.9 ±4. 9)d, (11. 3 ±0.7) weeks, the difference was statistically significant (all P＜0. 05). Antibiotic use of time,hip function(Harris score standard)excellent and good rate after half a year, there's no significant difference between the two groups( all P ＞ 0. 05). Complication rate, DHS group:40% ; PFN group: 10%. The difference was statistically significant (P ＜ 0. 05). Conclusion Intertrochanteric fracture fixation PFN group had an advantage of smaller surgical trauma,less bleeding,shorter healing time,more rapid postoperative recovery,lower complication rate and other characteristics comparing with the DHS group. So it was worth being recommended for a wider use.