Objective To study the clinical ralue of electromyogram in te sting peripheral nerves in diabetic patients and study the factors affecting per ipheral nearopathy. Methods FBG, 2HBG, HbA1c and electromyogram of nerves peronaeus communis and superficialis were measured in 2 3 diabetic patients. Result The result of electromyogram accord ed with clinical symptoms. Diabetic patients with neurological symptoms had higher voltage, prolo nged time limit of light contract peak value, and increased multiphase electric p otential compared with those without neurological symptoms (p

Objective To investigate clinical value of anti-adhesion agent:chitosan in preventing adhesions followed by laparotomy surgery in obstetrics and gynecology.MethodsFrom Jan.2006 to Dec.2009,770 patients underwent laparotomy surgery at Department of Obstetrics and Gynecology in the Second Affiliated Hospital of Harbin Medical University.One hundred and twenty-five patients underwent secondary surgery due to disease recurrence or cesarean section,the previous surgery were 18 cases with myomectomy,20 cases with endometriosis surgery,5 cases with resection of pelvic abscess,20 cases with ectopic pregnancy surgery,27 cases with benign adnexal neoplasm resection,9 cases with cyto-reductive surgery in ovarian cancer,26 cases with variable infertility surgery.Those 125 patients were managed by different agents washing before abdomen closure,which were assigned into two groups,including 59 cases washed by saline in control group and 66 cases washed by chitosan in study group.The abdomen adhesion in secondary surgery was evaluated by adhesion classification system.Results In study group,37 patients without adhesions,20 patients with degree Ⅰ adhesion,6 patients with degree Ⅱ adhesion,3 patients with degree Ⅲ adhesion,and no patients with degree Ⅳ adhesion were observed.While in control group,there were 11 patients without adhesions,23 patients with degree Ⅱ adhesion,14 patients with degree Ⅱ adhesion,8 patients with degree Ⅲ adhesion,and 3 patients with degree Ⅳ adhesion.The distribution of adhesion reached statistical significance between the two groups (x2 =20.9999,P =0.0003 ).Twenty-six patients in previous surgeries due to infertility included 17 cases in control group and 9 cases in study group.They all were managed by cesarean section in secondary surgery,it was found that 15 cases without adhesion and 2 cases with degree Ⅰ adhesion were in study group and 2 cases without adhesion and 7 cases with degree Ⅰ adhesion were in control group.It reached statistical difference ( P =0.0016 ).ConclusionAnti-adhesion agent could prevent adhesion followed by surgery in obstetrics and gynecology effectively.

Objective To study the inhibitory effect of Curcumin combined with Glycyrrhetinic acid on proliferation of HepG-2 cell,and probe the reasonability and scientificity of curcuma combined with glycyrrhiza.Methods The CCK-8 method was used to test the proliferation inhibition rate of HepG-2 cells after treated with Curcumin (10.00,5.00,2.50,and 1.25 μg/mL),Glycyrrhetinic acid (20.0,10.0,5.0,and 2.5 μg/mL) and Curcumin combined with Glycyrrhetinic acid in corresponding concentration for different time (8,16,or 24 h).Flow cytometry was used to detect the cell apoptosis and the cell cycle of HepG-2 cells after treated with Curcumin (5 μg/mL),Glycyrrhetinic acid (10 μg/mL) and Curcumin combined with Glycyrrhetinic acid in corresponding concentration for 24 h.Results Curcumin,Glycyrrhetinic acid and drug combination obviously inhibited the proliferation of HepG-2 cells in a time-and concentration-dependent manner,the effects of combination group was more stronger and showed additive effect or synergistic effect.The apoptosis rate of HepG-2 cells was significantly increased after treated with three groups of drug,combination group showed additive effect;Curcumin,Glycyrrhetinic acid and the drug combination showed significant G2 arrest.Conclusion Curcumin combined with Glycyrrhetinic acid has positive effect on inhabiting the proliferation and promoting apoptosis of HepG-2 cell.Curcuma combined with glycyrrhiza possess reasonability and scientificity.

Objective The purpose of this study was to explore the relationship between the loss of the gene deleted in colorectal carcinoma (DCC) gene expression in ovarian carcinoma and the transformation, progression of the tumor and its clinicopathological factors. Methods DCC gene mRNA expression were examined by reverse transcription polymerase chain reaction (RT-PCR) in 34 malignant, 10 benign and 10 normal ovarian samples. To clarify the expression of DCC gene by the DNA cloning and the DNA sequencing analysis in normal ovarian sample. Results The expression of DCC gene was lost in no normal ovarian tissues, in 2 (2/10) benign lesions, while the loss of DCC gene expression was found in 19(19/34,56%) carcinomas ( P

Objective To evaluate the inhibitory effect of endostatin on ovarian cancer cell line SKOV3 and to investigate the possible mechanism of the inhibition.Methods The effect of endostatin on SKOV3 cell proliferation was studied by means of MTT.The cell apoptosis was detected by transmission electronic microscopy.BCL-2 and BAX expressions were measured in SKOV3 cells treated by endostatin by immunocytochemistry,RT-PCR and Western blot.Results Endostatin inhibited SKOV3 cell proliferation(P

Background and purpose:Ovarian cancer is one of three common malignant tumors in the female reproductive system,whose mortality is the highest in all of the gynecological cancers.The genetic instability and hypermutation rate of tumor cells were found to be associated with chemotherapeutic resistance,and accounted for the high recurrence rate and the failure of current treatment.Invasion and metastasis of tumor are closely related to angiogenesis.Experiments in vivo and in vitro have confirmed that there are many kinds of promoter and suppressor of angiogenesis in primary malignant tumor cells.Angiogenesis could be a potential molecular target for cancer treatment.Endostatin is a specific inhibitor of vessel endothelium.Animal experiments have confirmed that Endostatin could inhibit tumor growth through anti-angiogenesis and result in the decrease of invasion and metastasis of cancer,and it would not easily induced drug resistance in the cells.Thus Endostatin could be safely used for a variety of cancer treatments,being able treat any patho-angiogensis.Recently it has been reported that Endostatin may significantly enhance anti-tumor effects when it is combined with traditional treatment methods such as surgery,chemotherapy,radiotherapy and immunotherapy.Although the study of Endostatin was done in vitro,the primary results were very encouraging and provide the rationale for future clinical trials.In this study,we aim to evaluate the inhibitory effect of Endostatin on SKOV_(3) cell and to investigate the possible mechanism of the inhibition.Methods:1.The effects of Endostatin on SKOV_(3) cells proliferation was studied by means!of MTT.2.The cell apoptosis and cell cycle were detected by transmission electron microscope and cell flow cytometry.3.bcl-2 and bax expression were determined in SKOV_(3) cell by immunocytochemistry,RT-PCR and Western blot analysis.Results:Endostatin inhibited SKOV_(3) cell proliferation,and the value of A_(490) of 15?g/ml 72 h Endostation group(0.454) was much lower than that of PBS control group(1.369)(P

Recurrent spontaneous abortion (RSA) was one of the most difficult infertile diseases which was usually defined as consecutive still birth for more than three times during the first 20 weeks of gestation. Various factors and pathogeneses were thought to play a role in RSA. Recent studies had indicated that the disorders of blood vessel growth and the secondary abnormal blood perfusion may contribute to the occurrence of RSA. However, blood vessel growth was linked with the regulation of angiogenesis related factors. To provide references for the clinical prevention and treatment, the purpose of this paper was to evaluate the association between solubility vascular endothelial growth factor receptor-1(sflt-1), thrombospondin 1(TSP-1) and ovary, endometrial abnormal blood perfusion in cases of RSA.

Objective To evaluate whether the timing of maternal hypoxia during pregnancy could myocardial remodeling in adult offspring.Methods Twenty-four pregnant rats were assigned to the maternal hypoxia group starting from the early period of pregnancy (G1,day 3 to 21 of pregnancy),the group starting from the middle period of pregnancy (G2,day 9 to 21 of pregnancy),the group starting from the late period of pregnancy (G3,day 15 to 21 of pregnancy),or the control group (G0).Six rats were included in each group.Rats in the maternal hypoxia groups (G1,G2 and G3) were subjected to hypoxia for 3 hours in a low pressure cabin with an oxygen concentration of (10± 1) ％.Systolic blood pressure,wet weight of the left ventricle normalized for body weight (left ventricular weight/body weight,LVW/BW),diameter of cardiomyocytes,and the expression levels of collagen Ⅰ and collagen Ⅲ were measured in adult male offspring at the age of 3 and 5 months.One-way ANOVA and SNK test were used for statistical analysis.Results Maternal hypoxia from day 3 to 21 of pregnancy induced higher systolic blood pressure in male offspring at the age of 3 months and 5 months [3 months:G1 (122.0± 11.7) mmHg,G0 (108.3±9.5) mmHg,F=2.97; 5 months:G1 (128.5±7.9) mmHg,G0 (114.6±-10.0) mmHg,F=3.17,both P＜0.05].Maternal hypoxia also led to a higher LVW/BW ratio (G1 1.99±0.03,G0 1.80±0.02,P＜0.05),and increased collagen Ⅰ and collagen Ⅲ expression (collagen Ⅰ /β-actin:G1 1.76±0.07,G0 0.48±0.04,P＜0.01; collagen Ⅲ /β-actin:G1 0.68±0.05,G0 0.34±0.03,P＜0.01) in adult male offspring at the age of 5 months.Maternal hypoxia from day 9 to 21 of pregnancy induced a higher LVW/BW ratio (3 months:G2 2.15 ± 0.05,G0 1.98 ± 0.02,P＜0.05; 5 months:G2 1.96±±0.05,G0 1.80±0.02,P＜0.05),and increased collagen Ⅰ and collagen Ⅲ expression (3 months:collagen Ⅰ /β-actin G2 0.98±±0.02,G0 0.87±0.02,P＜0.05; collagen Ⅲ /β-actin:G2 0.87±±0.01,G0 0.82±0.01,P＜0.05; 5 months; collagen Ⅰ /β-actin G2 1.61±0.05,G0 0.48±0.04,P＜0.01; collagen Ⅲ / β-actin:G2 0.61 ±0.04,G0 0.34±0.03,P＜0.01) in male offspring at the age of 3 months and 5 months,but did not affect systolic blood pressure.Maternal hypoxia from day 15 to 2l did not have any effect.The diameter of cardiomyocytes in male offspring was not significantly different between the groups.Conclusions Maternal hypoxia during different periods of pregnancy has different effects on cardiac structure in adult rat offspring.Maternal hypoxia started in early or mid-pregnancy leads to cardiac collagen deposition without an increase in myocyte size in adult offspring,which may be independent of the change in blood pressure.

Purpose To investigate the adverse reactions and management in the process of transvaginal real-time three-dimensional hysterosalpingo-contrast sonography (3D-HyCoSy).Materials and Methods A total of 976 patients with infertility accepting 3D-HyCoSy in Jiangsu Provincial Hospital of Traditional Chinese Medicine,Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,from December 2014 to August 2016 were retrospectively analyzed.The pain degree was evaluated using visual analog scale (VAS).Adverse reactions,including contrast countercurrent,vomiting,syncope and allergies,were recorded.The relationship between adverse reactions and ultrasound imaging signs was explored.Results There were 66 cases (6.76％) of VAS grade 0,346 cases of mild pain (35.45％),374 cases of moderate pain (38.32％) and 190 cases (19.47％) of severe pain.With the increase in the degree of tubal obstruction,the degree of pain gradually increased.Pain was the most common adverse reaction among patients,followed by vomiting (15 cases,1.54％),syncope (1 case,0.10％),and allergies (1 case,0.10％).The incidence of contrast countercurrent was 12.30％ (120 cases).The adverse reaction incidence of patients with abnormal ultrasound imaging signs was significantly higher than that of patients with normal signs (x2=29.02,P＜0.01).Conclusion With the extensive use of 3D-HyCoSy examination,its clinical application value has attracted a lot of attention,meanwhile,the understanding of its safety and related adverse reactions should also be improved.The state of patients should be closely observed before,during and after 3D-HyCoSy.Medical personnel should watch out for syncope and allergic reactions,and prepare for emergency management.

Objective To evaluate the relationship between the heart diastolic function and pulse wave velocity in patients with diabetes mellitus.Methods Fifty-five patients with diabetes mellitus underwent echocardiogram exam,PWV,and BNP determination.Patients were divided into two groups according to the results:the group with normal heart diastolic function(n =33)and the group with abnormal heart diastolic function( n =22).Another 30 healthy volunteers served as control group.The values of PWV were compared.between the three groups.The relationship between E/E' and PWV,BNP and PWV were evaluated with correlation analysis.Results The PWV value of abnormal heart diastolic function group was significantly higher than that of other two groups.PWV was positively correlated with E/E'and BNP ( r =0.58,P ＜ 0.05 ; r =0.63,P ＜ 0.05).Conclusion PWV is associated with the heart diastolic function.PWV can serve as an important factor to predict abnormal heart diastolic function in patients with diabetes mellitus.