Objective To investigate the free radical scavenging effects and antioxidation activity of squid(Dosidicus eschrichitii Steenstrup) skin gelatin hydrolysate.Methods The free radical scavenging effects of the hydrolysate on superoxide and hydroxyl free radicals were assessed by chemiluminescence analysis method.The activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and content of malondialdehyde(MDA),hydroxyprolyne(Hyp) in blood serum and skin tissue in galactose treated mice were analyzed after 42 days of hydrolysate feeding.Results and Conclusion The fraction with relative molecule weight less than 2000 D of the hydrolysate had the highest free radical scavenging effects on superoxide and hydroxyl free radicals,and the activities of SOD,GSH-Px were enhanced,the quantity of MDA in blood serum and skin tissue of the mice was decreased and the quantity of Hyp in skin tissue of mice was increased after the hydrolysate administration.

Nursing is an important component in the clinic of traditional Chinese medicine,therefore having been emphasized broadly and continuously by generations of doctors.However,because of the combination of the treatment and nursing in traditional Chinese medicine,nursing hash't formed its own system until now.Literatures on the knowledge of nursing in traditional Chinese medicine are also sporadically recorded.The author focused on the development of nursing in traditional Chinese medicine to demonstrate that nursing in China has a long-term history with plenty of contents and various techniques.

Guidelines as Topic ; Hand-Arm Vibration Syndrome ; diagnosis ; Humans ; Occupational Diseases ; diagnosis ; Reference Standards

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The article is to illustrate the application of teaching design,multi-media,network teaching and long-distance education,and the introduction of virtual reality techniques in medical teaching?

To understand the undergraduate students'psychological health condition of medical colleges and find countermeasures through the survey and analysis of the undergraduate students'psychological health condition of Hubei College of Traditional Medicine.

To increase the skill operant level of clinical skills,strengthening training proposal was carried out among the clinical speciality students before graduation practice.The problem existing in the skill operant teaching was analyzed and the corresponding measures ware provided at the same time in order to use them for the reference and make uninterrupted enhancement in the process of teaching in future.

OBJECTIVE: To improve the hospital drug batch management by using Hospital information system(HIS) so as to improve the management level on hospital drugs.METHODS: A rational and detailed operation processes for the stock out and stock-in of drugs was designed;The flow of drugs was accurately recorded by using multi-kind of tables and forms;Rules and regulations were consummated,operational processes were standardized and the internal control measure on drug management was perfected.RESULTS: With the HIS drug control system,the whole-range drug management from the procurement and inventory to the clinical use of drugs has been effectuated,the working efficiency and working quality have been enhanced,and a more standard and scientific management of drugs has been achieved.CONCLUSIONS: Drug batch management method is practical and scientific,but a more effective and consummated system is needed to guarantee its implementation.

OBJECTIVE:To develop an HPLC method for determination of main component in low concentration of alprostadil for injection. METHODS: Diode array detector was used for full wavelength scanning of the low concentration of alprostadil sample solution (20 ?g?mL-1) with detection wavelength set at 214 nm and the reference wavelength at 250 nm. The mobile phase consisted of acetonitrile-0.02 mol?L-1 KH2PO4 solution (pH 4.9,40∶60) at a flow rate of 1 mL?min-1. RESULTS: The linear range of alprostadil was 10～320 ?g?mL-1 (r=0.999 9) and the average recovery rate was 98.26% (RSD=1.68%). CONCLUSION: The method is of low cost and it is applicable for the determination of the low concentration of alprostadil for injection.

OBJECTIVE To investigate ?-lactamases genes in Pseudomonas aeruginosa(PAE) isolated from clinical patients.METHODS The drug-sensitivity to 16 antibiotics was detected by the disc agar diffusion(DAD) and microdilution.The related drug-resistance genes TEM,SHV,OXA-10,PER,VEB,GES,CARB,IMP,VIM,SPM,GIM,DHA,F-OX,MOX,CTX-M9,CTX-M1 and OprD2 were detected by PCR method.RESULTS Resistant rates of 34 MRPA to amikacin,ceftazidime,ciprofloxacin,cefoperazone/sulbactam,cefepime,imipenem,levofloxacin,piperacillin/tazobactam,aztreonam,piperacillin and tobramycin were 14.7%,29.4%,61.8%,67.6%,70.6%,73.5%,76.5%,76.5%,79.4%,91.2% and 94.1%,respectively,with 100% to others antibiotics.The positive rates of gene encoding extended-spectrum ?-lactamases FOX,IMP,DHA,and VIM were 23.5%,11.8%,11.8% and 5.9%.Twenty-four were deleted out outer membrane protein gene oprD2.Other ?-lactamase genes were absent in all isolates.CONCLUSIONS Clinical P.aeruginosa carries various ?-lactamases genes,with high rate of oprD2 gene.