Based on describing the development overview of the personalized medicine,biological and information technologies,the paper proposes that it is feasible to construct a personalized medical information system based on patient gene information,introduces the business process,structural design,data acquisition and database design of the system,gives an outlook for the future development of the system.

s in clinical education and medical education assessment/monitoring system.Authors hold that these factors will contribute to a growing quality momentum in its education and Success in refoms.

0.05),the overall response rate was 56.0% .Symptoms(18/25) related to cancer improved and side-reactions were tolerable. Conclusions:Treatment of relapse and metastatic colorectal cancer with combined chemotherapy based on oxaliplatin,irinotecan and 5-Fu by arterial intervention and iv drip is safe and effective,with only slight side-effects. This treatment can improve the symptoms and life quality of patients.

OBJECTIVE:To establish a HPLC method for the determination of main component and the related substances in pirenoxinum natricum eye drops. METHODS: A Inertsil C8-3 column was used with pH2.2 perchloric acid-methanol (35∶65) as mobile phase at a flow of 1.0 mL?min-1. The detection wavelength was 230 nm and the sample size was 10 ?L. RESULTS: Pirenoxinum natricum and the related substances could be baseline separated. The calibration curve was linear in the range of 49.5～990 ng(r=0.999 9). The detection limits was 89.3 pg, and average recovery was 99.1%, RSD was 0.42%. CONCLUSIONS: This method is rapid, accurate sensitive and can be used for the quality control of pirenoxinum natricum eye drops.

Objective A method was established for genotyping of hepatitis B virus (HBV),based on the restriction fragment length polymorphism(RFLP)created by BsrI, StyI,DpnI and HpaII action on an amplified segment of the S region. Methods 223 full-genomic sequences were analyzed and the aligned nucleotide and amino acid sequences of S gene, genotype specific regions were identified by the restriction enzymes, BsrI, StyI,DpnI and HpaII. Pre S PCR-RFLP genotyping method was applied to a number of serum samples from hepatitis B e antigen (HBeAg) positive and negative Chinese chronic HBV carriers. And in 90 samples the following genotypes were observed: 30B, 30 C, 30D. The method using S gene PCR-RFLP was confirmed to be correct by these 90 samples. Three samples of each genotype B, C and D were randomly selected and directly sequenced their S gene to confirm that HBV S gene PCR-RFLP genotyping method was correct disectly. Results The results of two PCR-RFLP HBV genotyping methods were coincide with that of S gene sequence. Conclusions The method for genotyping of hepatitis B virus (HBV), based on S gene RFLP is established to be highly sensitive, differential and accurate. The RFLP patterns are easy to be recognized because of its simplicity and singleness.

Objective To discuss the effect of small needle knife therapy on collagensⅠ and Ⅲ in the hypertrophic scar tissues that was subcutaneously transplanted into nude mice. Methods Six samples of human hypertrophic scar tissues without cuticle were subcutaneously transplanted into the back of 24 nude mice to create the animal models of hypertrophic scar. Ten days after of the operation,the mice were divided into control,0.1 mg/ml triamcinolone,0.2 mg/ml triamcinolone,and small needle-knife groups with 6 mice in each. Specimens of the scar tissues were collected in 14 days for immunohistochemistry to detect the collagen Ⅰ and Ⅲ. Results In all the groups,collagens Ⅰand Ⅲ distributed extensively in the cytoplasm of fibroblast and tissues. Image analysis showed that the concentration of collagens Ⅰand Ⅲ in 0.1 mg/ml and 0.2 mg/ml triamcinolone groups were significantly lower than those in the control (0.09?0.03,0.11?0.05 and 0.12?0.02,0.11?0.01 vs. 0.17?0.04,0.19?0.03,P0.05). Conclusions Small needle-knife therapy can reduce the concentration of collagens Ⅰand Ⅲ in transplanted hypertrophic scar tissues in nude mice.

Objective To explore the relationship between hepatitis B virus (HBV) genotype and outcome of HBV infection. Methods PCR amplification of HBV small S gene in combination with a restriction fragment length polymorphism (RFLP) analysis was applied to identify HBV genotype in the serum samples of 80 asymptomatic HBV carriers (AsC group) and 120 liver cirrhosis patients with HBV infection (LC group) in Gangdong province of China. Results The frequency of HBV genotypes B, C and D in AsC group was 45% (37/80), 33.75%(26/80) and 21.25%(17/80)respectively, and that of HBV genotypes B, C and D in LC group was 32.5%(39/120), 65.8%(79/120) and 1.6%(2/120) respectively. There is no HBV genotypes A, E and F in this series. The distribution of HBV genotypes in the two groups was not significant correlation with the state of HBeAg. There was a significant difference in the distribution of HBV genotypes between the two groups (P

Public participation is an indispensable link in the public health emergency management system, as well as a dynamic factor and a cornerstone. It undertakes the functions of early warning, emergency response and publicness maintenance, so that it values in improving the effectiveness of public health emergency management. The main paths to realize the value of public participation in the public health emergency management system are to be the gatekeepers of public health early warning and surveillance, the participants of the management and support of public health emergency prevention and control. It is a complex system to establish and improve the national public health system. Our society should pay attention to and give full play to the value of public participation.

Objective To investigate neuroprotective function of peroxisome proliferator-activated receptor gamma (PPARγ) agonist, rosiglitazone against reperfusion injury after focal cerebral ischemia in mice model.Methods To establish cerebral isebemia-reperfusion injury mice model, adult male mice underwent 2 hours of middle cerebral artery occlusion followed by 22 hours reperfusion (MCAO/R). One hour before MCAO/R, mice were treated with either vehicle (MCAO/R group) or rosiglitazone (6 mg/kg, rosiglitazone group). 2,3,5-triphenyhetrazolium chloride (TIC) staining was applied to determine the volume of cerebralinfarction.TheneurologicaldeficitwasscoredatZeaLonga 5-pointscale. Myeloperoxidase (MPO) activity was measured in brain tissue as an index of neutrophil accumulation. RT-PCR, immunohistochemistry and Western blot were performed to examine the mRNA and protein expression of pro-inflammatory mediators (ICAM-1, IL-1β and COX-2).Results (1) The volume of cerebral infarction in rosiglitazone group was significantly decreased from that of MCAO/R group ( 29. 1 ± 6. 6 vs 57.8 ± 9. 7 ,t = 5. 980, P < 0. 01 ), and rosiglitazone markedly improved neurological function in treated mice than MCAO/R mice(1.2 +0.4 vs 3.3 ±0.8, t =5.812, P<0.01). (2) Compared with MCAO/R group, MPO activity in the rosiglitazone-treated group was significantly lower ((0. 049 + 0. 005 ) U/g vs (0. 083 ±0. 008) U/g,t =5. 904, P <0. 01 ). (3) The mRNA and protein expression of pro-inflammatory mediators (ICAM-1, IL-1β and COX-2) in rosiglitazone group were also significantly decreased from those in MCAO/R group, as demonstrated by RT-PCR (0.313 ±0.024, 0.205 ±0.007, 0.359 ±0.060, t = 7.464, 19.656, 29.319, P <0.01, respectively) and Western blot (0.274±0.014, 0.205±0.025, 0. 146±0.015, t=79.909, 21.392, 95. 105, P<0.01, respectively). ConclusionThe present study suggests that PPARγ agonist, rosiglitazone, has neureprotective properties to cerebral ischemia-reperfusian injury and that the protection is partially mediated via anti-inflarmmatory actions.

Objective To understand the incidence of nosocomial infections for providing basis for the development of prevention and control measures.Methods All the hospitalized patients were investigated,data collection used by nosocomial infection surveillance system,the results were statistically analyzed.Results Nosocomial infections occurred in 46 case-times with the infection rate of 3.04％,the top 3 prevalence rates were in ICU(46.15％),department of hematology(21.87％),department of neurosurgery (1 1.76％).The main infection sites were lower respiratory tract (36.96％) and upper respiratory tract (28.26％).The utilization rate of antibiotics was 44.09％.There were 183 patients who received etiology examination with the submission rate of 32.28％.Conclusion The investigation of prevalence of nosocomial infections can contribute to understanding of the incidence of nosocomial infections,and taking interventions to the key departments,strengthening the clinical specimens submission,and standardizing the reasonable use of antibiotics can decrease the incidence of nosocomial infections.