In the teaching reform of cytobiology,we should first formulate a reasonable syllabus according to training goals,pay attention to course content reform,strengthen connection with other preclinical medicine subjects,combine quality-oriented education in class instruction,and cultivate students scientific and original spirit.The experimental courses should be offered from higher level and be conformed with subject development.The teacher should have innovative thinking,adopt diversified teaching methods,and fully arouse students' study enthusiasm.

Objective To investigate the relationship between the serum concentration of amiodarone and QT dispersion.Methods The serum concentration of Am and DEA were determined by HPLC during the treatment in forty Patients to study the relationship between the serum concentration and QTd.Results After using amiodarone,QT interval was significantly increased[(383±31)ms vs(418±29)ms,P＜0.05].In the same time QTd was significantly decreased[(34±12)ms vs(21±9)ms,P＜0.05].With maintaining dosage,the serum concentration of DEA had positive relationship with the increment of QT(r=0.50,P＜0.05).While with loading dosage,the serum concentration of amiodarone had no relationship with it.The serum concentration of amiodarone had no relationshiP with the decrement of QTd in all patients.And it varied in a large range.Between the groups of different therapeutic effect,the serum concentration had no statistical difference.Two patients appeared the adverse effects.Their serum concentration of amiodarone weren't higher than the average concentration of the group.Conclusion The serum concentration of amiodarone couldn't be an index tO estimate the effect and the occurrence of adverse effects.

A new method of the separation of hydrocarbons and glycerides in liver oil of Mustelus jriseas was designed. The Waters Prep-500A Liquid Chromatograph with silica gel column and differential refractive index detector was used. The mobile phase was petroleum ether and ethyl acetate. The pure hydrocarbons' and glycerides were obtained from the liver oil.

Tripterygium Hypoglaucum Hutch and Tripterygium Wilfordii Hook.f are all Evonymus alata family Tripterygium Wilfordii genus plants.They are generally used in the clinical treatment in department of dermatology.They resemble each other not only in some chemical constitutions,but also in some pharmacologic actions(such as anti-inflammatory,anti-tumor,immune suppression,antifertility and so on),but they are all poisonous plants.When they are used for therapy,they can induce some toxic reactions,even the death of patients.In order to facilitate the adoption of the two drugs,we review their pharmacologic actions.

Objective To probe into the application feature and use tendency of traditional Chinese medicine (TCM) injections in our hospital;To evaluate rational drug use and provide reference for related researches. Methods TCM injections in our hospital during 2010-2012 were analyzed by employing the analytic method of defined daily dose, with a purpose to evaluate frequency of drug use and rationality of medication expenses. Results From 2010 to 2012, drug possession rate of TCM injections in our hospital was 4.07%-4.47%;the amount of medication accounted for 10.50%to 12.69%of the amount of all drugs;the annual growth rate increased significantly, among which TCM injections used for removing blood stasis took the lead;frequency of drug use rose annually;medication synchrony in 3 years indicated that daily expensesof Shenkang Injection, Aidi Injection, salvianolateinjection, safflor yellow sodium chloride injection and other TCM injections were on the high side. Conclusion From 2010 to 2012, TCM injections in the clinical application in our hospital were of relatively high frequency, and the TCM injections used for removing blood stasis took the lead. Daily expenses of TCM injections drugs were on the high side and there may be unreasonable application.

Objective:To investigate the protective effect of punicalagin in LPS induced ALI.Methods: ALI mice model was induced by intraperitoneal injection of LSP(20 mg/kg bodyweight) to induce acute lung injury in mice.A total of 60 mice were divided into 6 groups,10 rats in each group:normal control group,LPS group,LPS+punicalagin group (10,20,40 mg/kg bodyweight),LPS+dexamethasone group.2 h before LPS injection,punicalagin and dexamethasone were intraperitoneal injection in mice,and then by intra-peritoneal injection of a lethal dose of LPS, normal control group mice were given intraperitoneal injection of an equal volume of PBS.HE staining,observed the lung tissue of mice in the different treatment conditions of the pathological changes and the degree of lung injury,weighted the wet lung and dry lung of the mice respectively, observed degree of pulmonary edema in mice, inspect MPO activity of lung homogenate, analyzed the activation and infiltration of polymorphonuclear, counted the total number of cells and the number of inflammatory cells in alveolar lavage fluid, detected the concentration of inflammatory cytokines in bronchoalveolar lavage fluid with ELISA,and analyzed the impact of punicalagin on inflammation of lung tissue.Results: HE staining of lung tissue in mice and found that the LPS-induced lung inflammatory cell infiltration, interstitial edema and pulmonary parenchymal damage were significantly reduced.Different dose of punicalagin could significantly reduce the lung wet/dry lung weight ratio of ALI in mice ( P<0.05).Punicalagin ALI could reduce lung tissue caused exudation,edema.The different doses of punicalagin could significantly reduce the inflammatory cells in bronchoalveolar lavage fluid,the sum of the number of neutrophils and macrophages (P<0.05).Punicalagin could significantly reduce the concentration in the bronchoalveolar lavage cytokines IL-6,IL-12,TNF-αand IL-1β(P<0.05).The MPO activity was detected that punicalagin reduce neutrophil infiltration induced by LPS in the lung tissue.Conclusion: Punicalagin has protective effects on LPS induced acute lung injury.

Objective: The clinical experience of treatment in 38 patients of Reinke′s edema by surgery of minimally invasive was reported from June 1997 to December 2000. Methods: Thirty-eight patients (25 males and 13 females, ranging in age from 46 to 67 years old) with Reinke′s edema of vocal cord were diagnosed by frequency-flash-laryngoscope and pathologic. The bilateral vocal cord stripping surgery with micro-suspension laryngoscope under general anaesthesia was completed in all 38 patients. Results: After treatment 3 months to 1 year follow-up in 33 cases, 27 cases(81.8%) had acquired good voice and only 6 cases (18.2%) had acquired lower tone voice. Conclusion: None of them had vocal cord adhesive with bilateral vocal cord mucous stripping operation which remained anterior 1/2 parts of vocal cord mucous on one side.

0.05);According to average 15 months follow-up,the complication rate was 0 and the excellent rate of shoulder fuction was 97.1% in the cases fixed with CHP,both were better than those of the cases fixed with K-wire of 38.9% and 72.2% respectively(P

Objective To study the relationship between carotid intima media thicknes(IMT) and cerebral infarction in patients with type 2 diabetes.Methods The IMT of the carotid artery was measured by high resolution B mode ultrasound, the relationships between carotid IMT and risk factors of cerebrovascular disease were evaluated.Results The serum levels of fibrinogen, total cholesterol and low density lipoprotein cholesterol were significantly higher in diabetic subjects with cerebral infarction than those in control group ( P

Chemotherapy plays an important role in advanced colorectal cancer. Patients may show various clinical response to selection different drugs. The importance of individual therapy based on pharmacogenomics has been emphasized in cancer therapy. To explore the individual therapy of colorectal cancer,the progress of molecular markers which influence the efficacy of anti-tumor drugs containing fluorouracil,oxaliplatin,CPT-11,monoclonal antibody drugs is reviewed in this article.