The ghrelin is an endogenous ligand of the growth hormone (GH) secretagogue receptor,which can regulate the secretion of GH,stimulate the appetite,adjust the energy metabolism,and improve the cardiovascular function.Children with congenital heart disease (CHD) usually suffered from malnutrition and growth retardation,which were connected with insufficiency of energy intake,poor absorption,and increase of nutritional requirement,anatomy and the changes of hemodynamics.Many infants with CHD gradually present ventricular dysfunction and growth restriction in their early childhood,which consequently lead to severe complications.Ghrelin is valuable in the early detection of potential CHD,plays an important role in improving the state of malnutrition and protecting the cardiac function,and provides an important reference to explore the new drug therapy for CHD.This review summarized the effects and significances of ghrelin on growth and cardiac function in infants with CHD.

Objective] To study the transmission of the Ishinpo versions ,understand the prevailing version of Ishinpo. [Method] With the research methods of the Chinese traditional history and philology,collect and sort historical cited references of the Ishinpo, by paying attention to three main emendation in the transmission history of Ishinpo. [Result] Ishinpo of Nakarai is the golden version for document research. The prevailing Ansei Ishinpo version of contemporary China is based on Nakarai version,which consists of twenty seven scrolls of Hei era and three scrolls of Edo Era. Ishinpo Versions have been divided into Emperor Nakarai versions,Uji versions and Family tradition versions. There are big differences in utilization and completeness of the three version system. The Emperor Nakarai versions are relatively good, of which the Nakarai version preserves best. The Uji versions are widespread, of which the RenHe Temple version is better preserved but lacuna. There are lots of lacunas in the family tradition versions, owing to circulateed copies. [Conclusion] In the three main emendation, of Ishinpo, mostly, the Nakarai version is the source edition, the Uji version is primary edition, and the family tradition version is the secondary edition. The paper, which is useful in the transmission of the Ishinpo and thus historical process of medical exchange between China and Japan, has established the system of Ishinpo versions, defined the golden version and the prevailing version.

Four methods for evaluating the academic impact of scholars were described according to their literature information, including subjective evaluation method, bibliometric method, social network analysis and comprehen-sive evaluation method .Their development trend was studied by analyzing their characteristics and applications .

1 5 cases,5 males and 1 0 females,aged 1 6-26 years,with ClassⅡdivision maxillary mild to moderate protrusion were includ-ed.Damon III appliance was used to arrange the upper and lower dentition.Stainless steel micro-screw implants were implanted in zygomatic crest to provide the best bone anchorage,stainless steel rectangular wire was used for maxillary complete dentition continuous ligation and maxillary teeth distal movement.X-ray cephalometry study showed that U1-NA(°)reduced by 2.51 ±1 .52(P<0.01 ),U1-NA(mm)re-duced by 4.56 ±1 .25,UL-EP(mm)reduced by 2.21 ±1 .83,U6E-PTV(mm)reduced by 2.93 ±1 .1 7,U6A-PTV(mm)reduced by 1 .56 ±0.95,SNA(°)and the mandibular parameters were not significantly changed(P>0.05),centric jaw relation of bilateral molar was obtained.

Objective To investigate the correlation between the serum levels of homocysteine (Hcy) ,folate acid (FA) and vita‐min B12 (VitB12 ) with cerebral infarction .Methods The enzymatic cycling method and chemiluminescence immunoassay were used to detect the serum Hcy ,FA and Vit B12 levels in 60 patients with cerebral infarction ,and then the detection results were compared the results of 40 people undergoing physical examination in the same period .Results The serum Hcy level in the cerebral infarction group was (23 .16 ± 8 .17)μmol/L ,which was significantly higher than (8 .09 ± 2 .45)μmol/L in the control group(P<0 .01) ,and the serum FA and Vit B12 levels in the cerebral infarction group were (6 .12 ± 2 .19) ng/mL and (323 .92 ± 133 .79)μmol/L ,which were in the control group lower than (10 .57 ± 5 .70) ng/mL and (530 .85 ± 168 .56)μmol/L in the control group (P<0 .01) .The serum Hcy level in the cerebral infarction group showed the negative correlation with FA and VitB 12 levels ( r1 = - 0 .444 , r2 = -0 .535 ,P<0 .05) .Conclusion High hyperhomocysteinemia is an independent risk factor of cerebral infarction and is related to the decrease of FA and VitB12 .

Objective To construct a new gene delivery system based on atelocollagen (ATE),and explore that modified aptamer (APT),and APT-ATE/miRNA (miRNA-15a and miRNA-16-1) were successfully synthesized to treat bone-metastatic prostatic cancers.Methods Flow cytometry (FCM) analysis was used to characterize APT-ATE complex.The diameter and zeta potential of complexes were measured by Zetasizer Nano-ZS9.The prostatic cancer (PCa) distribution experiments were used to explore its biological characteristics and targeting ability of PCa cells (PC3 and LNCaP).The inhibition of APT-ATE complex on LNCaP cell was determined with the cholecystokinin (CCK)-8 assay.Results FCM results demonstrated the successful synthesis of ATE-APT complex.The cellular uptake of vectors was concentration-dependent.The gene expression in vitro indicated that the modification of APT could increase the efficiency of gene expression and PCa targeting ability of ATE vectors to LNCaP [prostate specific membrane antigen (PSMA) over-expressing prostate cancer cells].The result of biodistribution showed that the bone uptake of APT-ATE was higher than ATE-APT.Conclusions APT-ATE/miRNA might be useful for preclinical and clinical studies on the treatment of bone-metastatic PCa.

Objective To study the T Lymphocyte Subsets and the cytokines in the newborns with infectious diseases. Methods Twenty cases of neonatal bacterial pneumonia (bacterial group), 15 cases of rotavirus enteritis (virus group) and 20 newborns with jaundice (control group) were recruited in this study. The peripheral CD4+T Cells and lymphocyte subsets were assessed by flow cytometry. Results The IL-4 level was significantly different among different groups (F=3.39, P=0.041). The levels of IL-17 and IFN-γdid not differ signiifcantly among different groups (F=0.28 and 1.24 respectively, P>0.05). The IL-4 level was higher in bacterial group than that in virus group and control group (P<0.05). The percentages of CD3+, CD4+, CD19+and NK cells and the ratio of CD4+/CD8+ had significant difference among different groups (F=3.30-26.69, P<0.05). The percentages of CD3+and CD4+cells and the ratio of CD4+/CD8+were lower in bacterial group and virus group than those in control group (P<0.05). The percentage of CD19+cells was higher in bacterial group and virus group than that in control group (P<0.05). The percentage of NK cells was lower in virus group than that in bacterial group and control group (P<0.05). Conclusions The pattern of cytokines level is different in newborns with infection caused by different pathogens. Newborns with infectious diseases have immune dysfunction and Th2-dominated imbalance. The low percentages of T lymphocyte subsets indicate the depressed cellular immunity after infection, which may result in atypical symptom and prolonged disease course.

Objective To establish the L929 cell line with high expression of asporin ,and to study the effect of asporin on the expressions of TGF- β1 and Col2α1 in mouse fibroblast L929. Methods Asporin cDNA was amplified from mouse mandible tissue by RT-PCR,then was inserted into the eukaryotic expression plasmid pcDNA3.1(-). The recombinant plasmid pcDNA3.1(-)-ASPNIII was transfected into the mouse fibroblast cell line L929, followed by G418 treatment. RT-PCR and Western blot assays were used to screen the stable, asporin high expressing L929 cell line.The effect of overexpression of asporin on the regulations of TGF-β1 and Col2α1 was further studied. Results The stable, aspori high expressing n L929 cell linewas successfully established. Results of RT-PCR and Western blot assays showed that overexpression of asporin could effectively inhibit the expressions of TGF-β1 and Col2α1. Conclusion Enforced expression of asporin can inhibit the expression of TGF-β1 and Col2α1 in L929 cells.

Proteomics is a new research area of the post-genomic era. Oncoproteomics is an important branch of it.Here we discuss the investigative outcome of thyroid oncoproteomics in recent years,such as specific tumor biomarkers and antineoplastic drug.

Melanoma-associated antigen MAGE-A3 is expressed in various types of tumors,but not in normal cells except testis and placenta.MAGE-A3 can be presented by both HLA class I and HLA class II molecules after being processed into antigen peptide.MAGE-A3 has recently been widely used in the diagnosis and immunotherapy of tumors.This article reviews the applications of MAGE-A3 in the diagnosis and immunotherapy of tumors.