Objective: To explore the effect of comprehensive yoga intervention program on cardiopulmonary function and physical fitness among female college students. Methods: Twohundred and forty female college students were invited to participate in the program and were randomly divided into intervention (n=120) and control group (n=120) by random number table method. The intervention group received regular yoga exercise, while the control group had no exercise intervention. Body shape, cardiopulmonary function, body flexibility, muscle strength and mental health of the two groups were compared before and after intervention. Results: The cardiopulmonary function indices except PP of the intervention group improved significantly after intervention. There were no significant changes in cardiopulmonary function before and after the intervention for control group. The HR values of the observation group were significantly lower, and the values of SV, CO, VC, MVV and FEV1/FEV were significantly higher compared with those of the control group(P<0.05). Body mass index(BMI), waisttohip ratio and body fat percentage of observation group were significantly lower than that of control group(P<0.05). The performance on the sitandreach and situp test of observation group were significantly improved after intervention and were significantly higher than the control group(P<0.05). Somatization, sensitivity, depression and anxiety in the observation group decreased significantly after intervention and were lower than that of the control group(P<0.05). Conclusion The comprehensive yoga intervention program can effectively improve body shape, body flexibility and muscle strength, as well as improve cardiopulmonary function. It also plays a key role in improving mental health among female college students.