In this study, a novel technique for the preparation of (125)I-5-trimethylstannyl-1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl) urail (FIAU) was developed, (125)I-FIAU biodistribution profile was detected in Kunming mice and the possibility of using FTAU radio-labeling for reporter gene imaging was explored. 5-trimethylstannyl-1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl) urail (FTAU) was labeled with radioiodine ((125)I). A rotary evaporation method was used to remove excess methanol. The reactant was purified through a Sep-Pak C18 reversal phase column. The radiochemical purity and in vivo stability were determined using silica gel thin layer chromatography (TLC). The biodistribution of (125)I-FIAU in Kunming mice was also detected. The results showed that (125)I-FIAU could be radiolabeled effectively with FTAU, with mean labeling rate being (81±0.38)% (n =5). The mean radiochemical purity of (98.01±0.40)% (n=5) was achieved after a reversal phase Sep-park column purification. (125)I-FIAU was stable when incubated in normal human serum or in saline at 37°C, with a radiochemical purity >96% during a 0.5-24 h time period. Biological experiments exhibited rapid clearance of (125)I-FIAU from the blood pool. (125)I-FIAU was mostly excreted by kidneys. (125)I-FIAU in myocardium dropped conspicuously after 8 h and there was hardly retention at 24 h. We were led to concluded that the new method of radioiodinization of FTAU for the preparation of (125)I-FIAU is easy, highly effective and stable in vivo. The biodistribution of (125)I-FIAU in Kunming mice showed it can serve as an imaging probe for myocardial reporter genes.

In this study,a novel technique for the preparation of 125I-5-trimethylstannyl1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl) urail (FIAU) was developed,125I-FIAU biodistribution profile was detected in Kunming mice and the possibility of using FTAU radio-labeling for reporter gene imaging was explored.5-trimethylstannyl-l-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl) urail.(FTAU) was labeled with radioiodine (125I).A rotary evaporation method was used to remove excess methanol.The reactant was purified through a Sep-Pak C18 reversal phase column.The radiochemical purity and in vivo stability were determined using silica gel thin layer chromatography (TLC).The biodistribution of 125I-FIAU in Kunming mice was also detected.The results showed that 125I-FIAU could be radiolabeled effectively with FTAU,with mean labeling rate being (81±0.38)％ (n =5).The mean radiochemical purity of (98.01±0.40)％ (n=5) was achieved after a reversal phase Sep-park column purification.125I-FIAU was stable when incubated in normal human serum or in saline at 37℃,with a radiochemical purity ＞96％ during a 0.5-24 h time period.Biological experiments exhibited rapid clearance of 125I-FIAU from the blood pool.125I-FIAU was mostly excreted by kidneys.125I-FIAU in myocardium dropped conspicuously after 8 h and there was hardly retention at 24 h.We were led to concluded that the new method of radioiodinization of FTAU for the preparation of 125I-FIAU is easy,highly effective and stable in vivo.The biodistribution of 125I-FIAU in Kunming mice showed it can serve as an imaging probe for myocardial reporter genes.

The endothelial glycocalyx(EG)is a polyglycoprotein complex present on the internal vascular surface,and its impairment is associated with the progression of multiple diseases,including atherosclerosis,stroke,sepsis,diabetes,kidney disease,hypertension,and lung edema.Therefore,glycocalyx health can be used as a biomarker to evaluate vascular health.Aging leads to dysfunctional changes in the glycocalyx;for example,its thickness decreases,and the genes of enzymes involved in its synthesis and digestion are dysregulated.As a natural barrier to the vascular system,age-related glycocalyx disruption is associated with vascular dysfunction,including impairment of vascular contraction and dilation,enhancement of permeability,dysregulation of inflammatory and immune reactions,and imbalance of anticoagulation and thrombin.From the perspective of'structure determines function'studies on the changing regularity of the thickness,components,microstructure,and mechanical properties of EG with aging and its relationship with vascular dysfunction are of great significance for the prevention,diagnosis,and treatment of atherosclerosis and other age-related cardiovascular diseases.