Objective: To understand the current situation of adolescents interactive health literacy in Yanbian poverty-stricken area and associated social-ecological factors, so as to provide scientific basis for targeted health education and policy adolescents in this area in the future. Methods: Using cluster sampling method, a questionnaire survey was conducted on 818 junior high school students in grades 7 and 8 of all 4 junior high schools in Antu County in November 2019. Results: The scores of interaction health literacy was in moderate level (101.38±20.12) in Yanbian poverty-stricken area. The average scores of all dimensions was (3.62±0.96), stress management (3.35±1.08), interpersonal relationship (3.29±0.82), nutrition (3.21±1.02), health awareness (2.99±0.82), physical activity (2.68±0.85). The results of multiple linear regression analysis showed that grade, gender, BMI, perceived health care, perceived academic performance, participation in extracurricular activities, types of guardians, parenting style; perceived school health policy; average screen time were associated with the level of adolescent interactive health literacy (P<0.05), explaining 54.5% of the total variation(F=35.97，P<0.01). Conclusion The health literacy level of adolescents in poverty-stricken areas is affected by personal, family environment, interpersonal relationship, school, media and other factors. It is recommended that future health literacy interventions for adolescents should consider the students themselves, as well as their socio-ecological environment.