With advances on drug genomics of leukemia,the drug toxicity has been found to be associated with the drug metabolism gene polymorphisms in children with acute lymphoblastic leukemia.Current researches are to focuse on how to find a genotype to predict methotrexate toxicity effectively and treat patients individually.This review illustrates the association between three drug-metabolizing enzyme gene polymorphisms including methylenetetrahydrofolate reductase,reduced folate carrer and glutathione S-transferase and toxicity of methotrexate.

Objective:To study the role of APACHEⅡscore pre-operation to infusion of blood preparation during the periods of hepatectomy.Methods:The correlation between APACHEⅡscore pre-operation and infusion of erythrocyte suspension and plasma were analyzed retrospectively in 104 cases who suffered from hepatectomy.Results:Those whose APACHEⅡscore were less than 5 had significantly lower volume of bleeding and lower infusion of erythrocyte suspension and plasma during the operation than those whose APACHEⅡscore were not less than 5(P

Objective To explore the efficacy and safety of hormone replacement therapy with combination of Fosamax and simple use of Fosamax in osteoporosis and steopenia of perimenopausal women.Methods One hundred and thirteen perimenopausal women with osteoporosis accepted treatment in Outpatient Clinic of Panyu Convalescent Hospital of Guangzhou from December 2010 to June 2014 were chosen and divided into the control group (n=55) and the observation group (n=58) according to therapeutic method.The control group was treated with Fosamax alone while the observation group was treated with HRT basing on the simple use of Fosamax.The therapeutic effect,lumbar and hip bone mineral density(BMD) level,tibial ultrasound velocity (SOS) and T values of tibia,gonadal hormone concentrations,endometrial thickness and adverse reaction of the two groups before and after the therapy were compared.Results (1) The total effective rate of the observation group after the treatment was 93.10％,significantly higher than tbat of the control group (78.18％,x2=3.829,P=0.034).(2) Before and after treatment in two groups,there were statistically significant differences in terms of lumbar spine BMD,hip BMD,SOS of tibia,blood follicle stimulating prime (FSH) and estradiol(E2) (P＜0.05),and there were statistically significant differences between the two groups after the treatment in terms of lumbar spine BMD,hip B MD,SOS of tibia,FSH and E2 (t =3.389,5.012,3.997,5.829,3.902;P=0.035,0.028,0.033,0.024,0.038).(3) There was statistically significant differences in term of the incidence of adverse reactions between the observation group and the control group (6.90％ vs.16.36％;x2=8.273,P=0.012).Conclusion Compared with alone use of Fosamax,the therapeutic effect of hormone replacement therapy with combination of Fosamax and in osteoporosis and steopenia of perimenopausal women is more significant with low occurrence rate of adverse reaction.It is worth popularization.

0.05),the clinical control rate were 67.65% and 52.94% (P

Objective:To investigate genetic polymorphism of four X chromosome short tandem repeats(X-STR)loci(DXS7132、 DXS6789,HPRTB,DXS8378)in Chongqing Han population.Methods:EDTA-blood samples were collected from the unrelated individuals in Chongqing.After being extracted with Chelex-100 method,the DNA samples were amplified by PCR technique.The PCR products were analyzed by PAGE,genotyped with silver stain method.Hardy-Weinberg equilibrium was tested,and then used direct counting method to calculate allele and genotype frequency of these four loci.Results:The polymorphisms of all four STR loci were obtained from 120 unrelated females and 91 unrelated males from Chinese Han group.?2 tests on the genotype frequencies in female reveal that genotype frequencies in females did not depart from Hardy—Weinberg equilibrium.Conclusion:It was suggested that DXS7132,DXS6789,HPRTB,DXS8378 locus are suitable for individual identification and paternity testing,and we provide new selectable STR markers for chromosome X haplotype in forensic application.

Malnutrition is highly prevalent in hospitalized children and has been associated with rele-vant clinical outcomes.Timely nutrition risk screening and reasonable nutrition support therapy subsequently for the high risk patients is the best and standard way.Five nutrition risk screening tools have been developed to identify the nutritional risk in hospitalized children.Even though nutrition risk screening is a necessary part of standard pediatric care,there are no international standardized screening tools for pediatric inpatients so far.The near future will provide us with comparative data on teh exi sting tools which may oc ntribute to deve-loping a standard for admitted nutrti ion risk screening in pediatrics.

Objective To explore the clinical value of Holter heart rate turbulence in diagnosis of patients at high risk for coronary heart disease.Methods 110 patients with suspected coronary artery disease,according to the results of coronary angiography (CAG),were divided into CAG positive group (67 cases) and CAG negative group (43 cases).Heart rate turbulence and heart rate variability were analyzed in two groups.The atrial fibrillation,atrial flutter,and pseudo-differential interference were removed,the qualified ventricular contraction was selected.Two parameters of HRT,the initial shock value (TO) and turbulence slope value (TS) were calculated.Results The average value of TO and TS was (0.36 ± 0.15) ％,(1.98 ± 0.52) ms/RR in positive group,those in the negative group were (-0.87 ± 0.36) ％ and (4.83 ± 0.65) ms/RR,there were statistically significant differences between two groups (t =1.953,1.976,all P ＜ 0.05).After 1 year follow-up,9 cases (13.44％) died in positive group,2 cases (4.65％) died in negative group,the difference was statistically significant (x2 =5.476,P ＜ 0.05).Conclusion The heart rate turbulence with dynamic cardiogram can help to early diagnose the patients at high risk of coronary heart disease.It was important to improve the survival rate of patients.The method is simple,inexpensive,effective,which is worth promoting in the clinic.

Objective:Through research of corneal transparency and related influential factors,the authors try to look for some clinical variety regulations after penetrating keratoplasty(PKP) in order to enhance the rate of surgery success.Methods:104 blind patients due to corneal disease (107 eyes) undergone PKP were followed up on months 1,3,6,12,18,24 and 36.Clarity of cornea,corneal endothelial cells and all kinds of complications were recorded besides regular examinations.Results:Grafts were transparent in 82 of 107 eyes on month 1(76.6%),in 69 of 90 eyes on months 6 (76.7%),in 51 of 81 eyes on months 12(63%),in 26 of 40 eyes on months 12 (65%) after PKP.Corrected vision were improved especially in patients of grafts transparency.Vision of patients with transparent grafts were more than 0.1 in 67 of 82 patients(82%) on month 1,in 59 of 63 patients(94%) on months 3,in 66 of 69 patients(96%) on months 6.Conclusion:PKP is an important method to corneal disease blind patients.Rejection was the major cause affecting graft transparency.

Objective To determine the content of rutin in Circanetten New tablets by HPLC. Methods The determination is performed at C18(150 mm? 4.6 mm, 5 ? m) column with temperature being 30 ℃ ; the mobile phase is consisted of methanol- water- glacial acetic acid(40 ∶ 60 ∶ 1.8) and UV detection wavelength is at 354 nm. Results The linear range was 6.008 to 48.064 ? g/mL( r=0.999 9) for Circanetten New tablets and the average recovery of rutin was 100.3% (RSD=0.5 % , n=5) .Conclusion The method is simple and effective, and can be used to control the quality of Circanetten New tablets.

Objective:To analyze and determine the main component in volatilization oil which is extracted from the Chinese herbal medicine Kaempferia galanga L.Methods:Volatilization oil is obtained by steam-distillation from commercial available Kaempferia galanga L..After re-crystallization,GC-MS,UV-Vis,NMR,IR were processed for analysis and structure determination.Results:The m/z=205 peak in MS represents the quasi-molecular ion peak.The strongest absorption peak at 304nm in UV-Vis shows that there is big conjugated system in the molecule.Along with the data of chemical shifts and coupling constants of 1H-NMR,13C-NMR and wave numbers of IR absorption peaks,the main ingredient of Kaempferia galanga L.volatilization oil is verified as ethyl 3-(4-methoxyphenyl)-(E)-acrylate.Conclusion:We have employed advanced methods in separation and analysis in the determination of structure of the main ingredient in volatilization oil extracted from Kaempferia galanga L.and get detailed data to support the results,the methods are reliable and the results are dependable.