China ; Fluorine ; analysis ; toxicity ; Humans ; Neoplasms ; mortality ; Water Supply ; analysis

0.05). However the maximum enhancement index and contrast distribution volume in tumour and the maximum enhancement index of normal PZ in the post TURP group were significantly higher than those determined prior to surgery (?

Objective　To compare the effects of ouabain and digoxin on both the systolic blood pressure (SBP) and sodium pump α-subunit expression in myocardium of rats. Methods　Normal Sprague-Dawley (SD) rats were injected with ouabain, digoxin or normal saline (NS) everyday and indirect SBP was recorded once a week. Six weeks later, all the rats were killed and sodium pump α1-, α2-, and α3-subunit were detected in the left ventricular myocardium with RT-PCR method and immunohistochemical assay at mRNA and protein levels. Results　At the end of six weeks SBP of rats infused with ouabain increased significantly (132.6±9.0 vs 115.7±8.2 mm Hg， P＜0.01), while no difference of SBP was found between digoxin group and NS group. The effects of ouabain and digoxin on sodium pump α-subunit isoform expression in myocardium were also different: both ouabain and digoxin stimulated expression of α3-isoform whereas α2-isoform unchanged at both mRNA and protein levels. α1-isoform was decreased in ouabain group and α1-isoform unchanged in digoxin group at both mRNA and protein levels. Conclusion　It is suggested that both ouabain and digoxin could regulate sodium pump α-subunit isoform expression, which might be related to the physiological roles of endogenous ouabain and might be responsible for the difference between the pharmacological and toxicological effects of ouabain and digoxin, including their effects on blood pressure.

Objective To study the relationship between endoscopic and histologic mucosal changes in chronic gastritis.Methods All 403 patients with chronic gastritis were examined by endoscopy and two samples of gastric mucosa were examined by the same pathologist.Results (1)Gastroscopic results:there were 380 cases of chronic superficial gastritis(CSG),38 cases of chronic atrophic gastritis(CAG) including 15 cases of coexistence of CSG and CAG.(2)Pathologic results:365 cases of CSG;49 cases of CAG including 11 cases of coexistence of CSG and CAG.Among 403 cases,108 cases with intestinal metaplasia(IM),59 cases with mucosal erosion.39 cases had active chronic gastritis(ACG).(3)For CSG,comparing with pathology,endoscopic results had 95.3% of sensitivity and 91.6% of positive predictive value.In contrast their specific rate and negative predictive value were 15.8% and 26.6%,respectively;for CAG,endoscopy had 96.0% of specific rate and 93.2% of negative predictive value for pathologic diagnosis.Its sensitivity and positive predictive value were 46.2% and 63.2% respectively.(4)Comparing with pathologic results,endoscopic features had less sensitivity(50%),but some had a higher positive predictive value for pathologic CSG,CAG,and IM.(5)CAG was most likely associated with type Ⅱ IM(85.2%).Although most type Ⅰ IM was presented in CSG(81.0%).Conclusion Any single endoscopic finding was nonspecific for pathologic diagnosis;although some charactristical endoscopic findings had a higher positive predictive value. In other words,a single pathologic change may associated with various endoscopic findings, while a single later finding only presented one or two pathologic changes.

Objective To confirm the role of HLA B2704 in the pathogenesis of psoriasiform dermatosis by investigating the phenotype of skin diseases in HLA B2704 transgenic mice.Methods The transgenic positive mice were screened and identified by PCR,dot blot and Southern blot hybridization.HE and Immunohistochemistry staining of the diseased mice skin were performed to detect the pathological changes and expression of HLA B2704.Results Six transgenic positive mice developed obvious psoriasiform dermatosis.Histologically,the skin was massively thickened by psoriasiform hyperplasia and keratinization.HLA B2704 antigen was highly expressed on the cell surface.Conclusion HLA B2704 heavy chain can induce the psoriasiform dermatosis in the transgenic mice.

Aim To determine what effects propofol exerts on apoptosis in hippocampal neurons caused by anoxia-reoxygenation.Methods Newborn SD rats(0.05).When pretreated with propofol,neurons proliferation increased and apoptosis decreased(P

Objective To explore surgical treatment through posterior approach to reduce the fracture and dislocation complicated with longitudinal ligaments and intervertebral disc rupture in the thoracic or lumbar spine. Methods Eighteen cases suffered from the fracture and dislocation complicated with longitudinal ligaments and intervertebral disc rupture in the thoracic or lumbar spine between January 2001 and May 2004, retrospectively were reviewed all the patients are male, aged from 19 to 58 years old (average 38 years). All the patients are paraplegia. The involved vertebrae are T11 to L 3. All the lateral X-ray films showed the fractured vertebra taking on wedged shape. The posterior vertebral body-height was decreased in six patients. The fractured vertebra lost its height by 1/4 to 3/4 of normal height. The upper vertebral dislocation extent was from 25% to 100%. Two cases complicated with lateral displacement. All the patients were operated on within posttraumatic 10 days. One of the two posterior surgical techniques was used for a given patient: 1) One technique is implantation of pedicle-screws in upper and lower vertebrae just adjacent to fractured vertebra. 2) Another technique is implantation of pedicle-screws both in fractured vertebra and its adjacent upper and lower vertebrae. Results All the patients were followed up 3 months to 3 years (average 18 months). 8 cases were treated with two-vertebrae 4 pedicle-screws implantation technique, the height of fractured vertebral body was not improved in 5 cases, partially improved in 3 cases. The dislocation of the upper vertebra adjacent to fractured vertebra was partially improved in 6 case, not changed in 1 case, got worse in 1 case. 10 cases were treated with three-vertebrae 6 pedicle-screws implantation technique, the height of fractured vertebral body was all improved by 80% to 100%. The dislocation of the upper vertebra was corrected completely in all the ten cases. Conclusion The procedure of three-vertebrae 6 pedicle-screws implantation is practical, effective and useful for treatment of the fracture and dislocation comlicated with longitudinal ligaments and intervertebral disc rupture in the thoracic or lumbar spine.

Objective To study immune function of peripheral blood dendritic cells in chronic hepa titis B virus infected patients. Methods Peripheral blood DCs of patients and normal human were isolated and cultured in serum free media. The expression levels of DC surface molecule were analyzed by flow cytometry and the ability of DC to induce T lymphocyte proliferation were evaluated by a liquid scintillation counter and the amounts of cytokines in MLR were measured detected. Results The expression rate of CD86 (70.2?5.2)% on DC in patients was decreased compared with that in controls. (95.3?3.5)%, P

Objective To evaluate the MR findings of normal and abnormal collateral ligament. Methods The MR findings of 56 normal and 36 surgically confirmed injured collateral ligaments were retrospectively reviewed and compared. Results The normal tibial and fibular collateral ligaments were hypointense string on both T 1 and T 2 coronal MR images, with an average length of 6.8 cm for tibial collateral ligament and 5.7 cm for fibular collateral ligament. 36 collateral ligaments were injured, including 18 tibial and 12 fibular collateral ligaments. Three cases (6 ligaments) had both tibial and fibularcollateral ligaments injuries. The injury of collateral ligament was classified as: Grade I (18 ligaments), Grade Ⅱ (11 ligaments), and Grade Ⅲ (7 ligaments). The complications of anterior cruciate ligament sprains (13 cases), posterior cruciate ligament sprains (9 cases), meniscal tears (12 cases), bony contusion and fracture (7 cases), and joint effusion (20 cases) were found in 25 patients(78%). Conclusion Coronal MR imaging can clearly demonstrate the structure of the knee collateral ligaments, accurately diagnose and classify the ligament injury, and correctly detect the complications. MRI should be used as a routine examining method for knee trauma.

Objective To investigate the pathological features of Lewy body disease. Methods An autopsy case of an 80 year old Chinese woman suffering from Lewy body disease was studied The patient had progressive dementia and myotonia for three years and was suspected with Alzheimer dementia clinically Brain tissue was analyzed neuropathologically by using HE staining and specific staining for nervous system, immunohistochemical staining for ubiquitin and by using ? synuclein and electronic microscopy. Results Neuropathological examination revealed the most characteristic features as follows: (1) The number of melanin pigmented neurons were markedly decreased in the substantia nigra and locus ceruleus, and some typical brain stem type Lewy bodies in the residual neurons with moderate gliosis were found (2) There appeared numerous cortical type Lewy bodies in deep layers of the cerebral cortex particularly in the cingulate gyrus, hippocampus, parahippocampal gyrus, transentorhinal contex, amygdala and insula (3) There were selective spongiform changes and many Lewy related neurites in positive ubiquitin immunohistochemical staining on the CA2/3 sectors, parahippocampal gyrus and transentorhinal cortex (4) A strong positive ? synuclein immunoreactivity was found in all Lewy bodies and Lewy related neurites (5) Alzheimer disease related changes such as senile plaques and neurofibrillary tangles were scarcely found only in hippocampus and parahippocampal gyrus. Conclusion Lewy body disease is a new group of degenerative disease of the central nervous system ? synuclein may play an important role in the degeneration of neurons and their neurites may be a major component of Lewy bodies in Lewy body disease It is considered that the present case should be a pure type of diffuse Lewy body disease