AIM: To study the preventive effect of fibronectin on hepatic failure induced by endotoxin in mice.METHODS: The survival rate was observed in endotoxemia mice injected with fibronectin from human plasma. The tissue damage and expression of TNF?, IL-1?, IL-6 mRNA in hepatocyte were detected by the methods of histology, ultrastructure, DNA fragementation and RT-PCR. RESULTS: ①Fibronectin obviously reduced the mortality of endotoxemia mice sensitized by D-galactosamine(GalN). ②Histopathology showed that less necrosis occurred on the hepatocyte of endotoxemia mice injected with fibronectin, compared with saline control. ③Ultrastructure and DNA fragmentation showed fibronectin suppressed hepatocyte apoptosis induced by LPS. ④Fibronectin down-regulated the overexpression of TNF?, IL-1?, IL-6 mRNA on hepatocyte induced by LPS. CONCLUSION: Fibronectin supports endotoxemia mice surrival by down-regulating the expression of TNF?, IL-?, IL-6, it may be a potent therapy for endotoxemia.

Objective:To establish a feasible method for extracting polysaccharide from the discarded fibrous roots of Radix Panacis Quingueforlii afterpanaquilon had been extracted, and determine the polysaccharide content. Methods:Enzymolysis technique and alcohol was applied for decolorization, and phenol-sulfuric-acid method was used to determine the active polysaccharide content.The content of trace element and heavy mental was measured by element-analyzer and atomic fluorescence photometer respectively. Results: The yield of polysaccharide from the fibrous roots was close (about 11.7%) to the main root.And the content of heavy metal can match the national standard.Conclusion:It is valuable to extract the polysaccharide from the discarded fibrous root of Radix Panacis Quingueforlii.

Objective: To establish the quality standard of Jinwang Capsules.Methods: The technique of TLC was used to identify 10-Hydroxy-2-decylenic acid (10-HDA). Its content was determined by dual-wavelength UV spectrophotometry.Results: 10-HDA can be detected by TLC. The content of 10-HDA wasn't lower than 3.0mg per granule. Volatile alkalescent substance wasn't more 100mg per 100g.Conclusion: These methods are able to effectively control the quality of Jinwang Capsules.

Objective To investigate TEM and SHV ESBLs in Klebsiella pneumoniae and Escherichia coli clinical isolates from West China Hospital of Sichuan University and detect resistance of ESBLs-producing isolates. Methods The TEM and SHV ESBLs-encoding gene was amplified by PCR and was sequenced. And the MIC of eight antibiotics against the ESBLs-producing strains were detected by agar dilution. Results All strains were resistant to cefotaxime; eleven strains were resistant to aztreonam; two were resistant to ceftizidime; eleven, five and three were resistant to ciprofloxacin, amikacin and cefoxidine respectively; All strains were susceptible to imipenem. Ten strains of twelve ESBLs-producing strains carried bla SHV-2, two carried bla TEM-19. Conclusions ESBLs producers were mainly resistant to cefotaxime and aztreonam and most of them were multi-drug resistance; Cefotaxime resistance is partially due to production of SHV-2 and TEM-19 in this study.

Objective:To establish a feasible method for extracti ng polysaccharide from the discarded fibrous roots of Radix Panacis Quingueforli i afterpanaquilon had been extracted, and determine the polysaccharide content. Methods:Enzymolysis technique and alcohol was applied for decolorization, and ph enol-sulfuric-acid method was used to determine the active polysaccharide conte nt.The content of trace element and heavy mental was measured by element-analyze r and atomic fluorescence photometer respectively. Results: The yield of polysac charide from the fibrous roots was close (about 11.7%) to the main root.And the content of heavy metal can match the national standard.Conclusion:I t is valuable to extract the polysaccharide from the discarded fibrous root of R adix Panacis Quingueforlii.

Objective To discuss diagnostic classification and emergency treatment protocols for complex pelvic fractures. Methods The first aid management of 23 cases of major pelvic fractures were analyzed retrospectively. Results 22 of the patients survived, but one died of hemorrhagic shock.Conclusions Correct classification of severe pelvic fractures is of guidance value to the first aid management. Cooperation among multiple departments, comprehensive application of various surgical methods, early stabilization of hemodynamics, rapid repair of injured organs, stabilization of pelvic ring with external fixator are effective techniques to enhance the survival rate of patients with severe pelvic fractures.

AIM: To explore the relationship between methylation status of promoter region 5′CpG island and the biological phenotype in human colorectal cancer RKO cell lines. METHODS: RKO cells were treated with selective DNA methyltransferase (DNMTs) inhibitor, 5-Aza-2′-deoxycytidine (5-Aza-CdR), for 72 h. Methylation-specific PCR (MSP), T-A clone and DNA sequence analysis were used to detect 5′CpG island methylation status of p16/CDKN2 tumor suppresor gene. Cell growth, cell cycle arrest and apoptosis were analyzed by MTT, flow cytometry (FCM), fluorescent dye staining and transmission electron microscope. RESULTS: DNMTs inhibitor (5-Aza-CdR) effectively reversed the hypermethylation status of 5′CpG island. The effects of 5-Aza-CdR on cell growth inhibition (P

AIM: To study apoptosis in heart of multiple organ dysfunction syndrome (MODS) rat at high altitude. METHODS: The two-hit model of MODS rat was used at two different altitude(1 510 m, 3 900 m). Hemorrhage was induced in Wistar rats by catheterizing the femoral artery until a mean arterial pressure was 35 mmHg and maintained for 1 hour. Rususcitation was performed with lactated Ringer′s solution at 24 h after hemorrhage, cecal ligation and puncture(CLP) was performed .Then rats were killed at 3 h,6 h,12 h and 24 h after CLP, and myocardium sample was excised and stored in liquid nitrogen. Apoptosis in heart was determined by DNA agarose gel electrophoresis, flow cytometry(FCM), transmission electron microscope(TEM) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL). RESULTS: The special ladder pattern for apoptosis was seen in myocardium sample at high altitude(3 900 m) group.The apoptotic rate in myocardium was higher in high altitude(3 900 m) group than that in lower altitude(1 510 m) group( P

AIM: To observe the effect of antisense bcl-2 oligodeoxynucleotides(AS-PS-ODN) on bcl-2 mRNA and protein expression, cell proliferation,viability and apoptosis in a small-cell lung cancer cell line NCI-H446. METHODS: Semi-quantitative RT-PCR was performed to detect the bcl-2 mRNA expression, the Bcl-2 protein was determined by immunocytochemistry and flow cytometry analysis, and the effect of bcl-2 AS-PS-ODN on cell proliferation, viability and apoptosis were investigated by colony assay , cell count, DNA content analysis and TUNEL. RESULTS: ① 1 ?mol/L bcl-2 AS-PS-ODN significantly down-regulated the expression of bcl-2 mRNA and protein. The inhibition rate of mRNA and protein were 69.5% and 62.7%, respectively. ② bcl-2 AS-PS-ODN decreased cell proliferation and viability , induced cell apoptosis.The apoptosis rate was 22.3%-32.7% in cells treated with 1?mol/L bcl-2 AS-PS-ODN. CONCLUSION: bcl-2 AS-PS-ODN down-regulated expression of bcl-2 mRNA and protein, inhibited cell proliferation and induced apoptosis in a small cell lung cancer cell line, NCI-H446.

Objective Serum pharmacochemistry was performed to screen the bioactive constituents of Resina Draconis.Methods Based on HPLC fingerprints of Resina Draconis,the migrating constituents absorbed into blood were determined by comparing the HPLC fingerprints of extraction of Resina Draconis(ERB),herb serum sample,and control serum sample,and with the help of LC-MS/MS.Results Six compounds absorbed into blood were detected,five of them were original in form which were 3,4′-dihydroxy-5-methoxystilbene,cochinchinenin B,4′-hydroxy-4,2′-dimethoxy-dihydrochalcone(a new compound),cochinchinenin A,and loureirin B,respectively.The other might be the original constituent or the metabolite.Conclusion The six constituents absorbed into blood are possible bioactive components of Resina Draconis in vivo.Further research will help clarify the bioactive constituents and mechanisms of Resina Draconis.