1.Blood-retina barrier dysfunction in experimental autoimmune uveitis: the pathogenesis and therapeutic targets
Jeongtae KIM ; Jiyoon CHUN ; Meejung AHN ; Kyungsook JUNG ; Changjong MOON ; Taekyun SHIN
Anatomy & Cell Biology 2022;55(1):20-27
Experimental autoimmune uveitis (EAU), an animal model of human uveitis, is characterized by infiltration of autoimmune T cells in the uvea as well as in the retina of susceptible animals. EAU is induced by the immunization of uveitogenic antigens, including either retinal soluble-antigen or interphotoreceptor retinoid-binding proteins, in Lewis rats. The pathogenesis of EAU in rats involves the proliferation of autoimmune T cells in peripheral lymphoid tissues and breakdown of the blood-retinal barrier, primarily in the uvea and retina, finally inducing visual dysfunction. In this review, we describe recent EAU studies to facilitate the design of a therapeutic strategy through the interruption of uveitogenic factors during the course of EAU, which will be helpful for controlling human uveitis.
2.No association of prion protein gene polymorphisms with Alzheimer's disease in Korean population.
Kyungsook AHN ; Eunkyung KIM ; Young A KWON ; Doh Kwan KIM ; Jong Eun LEE ; Sangmee Ahn JO
Experimental & Molecular Medicine 2006;38(6):727-731
The polymorphism at codon 129 (M129V) of the human prion protein gene (PRNP) is a known risk factor for Creutzfeldt-Jakob disease (CJD) in Caucasians. There are few reports of this polymorphism's effect on memory and on the risk of Alzheimer's disease (AD). The M129V genotype distributions among Asians are very different from Caucasians. Another polymorphism, codon 219 (E219K) is not found in Caucasians. We investigated two polymorphisms of PRNP, M129V (rs1799990) and E219K (rs1800014) in 297 Korean AD patients and 217 healthy subjects. The analysis of the genotype and allele distributions showed no significant difference between the AD patients and the controls in both polymorphisms (P=0.19 genotype, P=0.51 allele for M129V; P=0.64 genotype, P=0.50 allele for E219K). Also, the PRNP polymorphisms were not significantly associated with AD when the populations were stratified for the presence or absence of apolipoprotein E-e4 (ApoE-epsilon4) allele. These results suggest that the PRNP genetic variants are not associated with the risk for AD in Korean population.
Prions/*genetics
;
Polymorphism, Genetic/*genetics
;
Male
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Korea/epidemiology
;
Humans
;
Genotype
;
Genetic Predisposition to Disease/genetics
;
Female
;
Codon/genetics
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Apolipoproteins E/genetics
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Alzheimer Disease/*epidemiology/*genetics
;
Alleles
;
Aged
3.Appearance of osteoporosis in rat experimental autoimmune encephalomyelitis.
Meejung AHN ; Sohi KANG ; Channam PARK ; Jeongtae KIM ; Kyungsook JUNG ; Miyoung YANG ; Sung Ho KIM ; Changjong MOON ; Taekyun SHIN
Korean Journal of Veterinary Research 2016;56(2):117-120
Experimental autoimmune encephalomyelitis (EAE) in Lewis rats is characterized by transient paralysis followed by recovery. To evaluate whether transient paralysis in EAE affects bone density, tibiae of EAE rats were morphologically investigated using micro-computed tomography and histology. The parameters of bone health were significantly reduced at the peak stage of EAE rats relative to those of controls (p < 0.05). The reduction of bone density was found to remain unchanged, even in the recovery stage. Collectively, the present data suggest that osteoporosis occurs in paralytic rats with monophasic EAE, possibly through the disuse of hindlimbs and/or autoimmune inflammation.
Animals
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Autoimmunity
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Bone Density
;
Encephalomyelitis, Autoimmune, Experimental*
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Hindlimb
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Inflammation
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Osteoporosis*
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Paralysis
;
Rats*
;
Tibia
4.Potential involvement of glycogen synthase kinase (GSK)-3β in a rat model of multiple sclerosis: evidenced by lithium treatment.
Meejung AHN ; Jeongtae KIM ; Changnam PARK ; Jinhee CHO ; Youngheun JEE ; Kyungsook JUNG ; Changjong MOON ; Taekyun SHIN
Anatomy & Cell Biology 2017;50(1):48-59
Glycogen synthase kinase (GSK)-3β has been known as a pro-inflammatory molecule in neuroinflammation. The involvement of GSK-3β remains unsolved in acute monophasic rat experimental autoimmune encephalomyelitis (EAE). The aim of this study was to evaluate a potential role of GSK-3β in central nervous system (CNS) autoimmunity through its inhibition by lithium. Lithium treatment significantly delayed the onset of EAE paralysis and ameliorated its severity. Lithium treatment reduced the serum level of pro-inflammatory tumor necrosis factor a but not that of interleukin 10. Western blot analysis showed that the phosphorylation of GSK-3β (p-GSK-3β) and its upstream factor Akt was significantly increased in the lithium-treated group. Immunohistochemical examination revealed that lithium treatment also suppressed the activation of ionized calcium binding protein-1-positive microglial cells and vascular cell adhesion molecule-1 expression in the spinal cords of lithium-treated EAE rats. These results demonstrate that lithium ameliorates clinical symptom of acute monophasic rat EAE, and GSK-3 is a target for the suppression of acute neuroinflammation as far as rat model of human CNS disease is involved.
Animals
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Autoimmunity
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Blotting, Western
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Calcium
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Central Nervous System
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Central Nervous System Diseases
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Encephalomyelitis, Autoimmune, Experimental
;
Glycogen Synthase Kinase 3
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Glycogen Synthase Kinases*
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Glycogen Synthase*
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Glycogen*
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Humans
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Interleukin-10
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Lithium*
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Models, Animal*
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Multiple Sclerosis*
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Paralysis
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Phosphorylation
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Rats*
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Spinal Cord
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Tumor Necrosis Factor-alpha
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Vascular Cell Adhesion Molecule-1
5.Gene Expression Profile of Olfactory Transduction Signaling in an Animal Model of Human Multiple Sclerosis
Jeongtae KIM ; Meejung AHN ; Yuna CHOI ; Poornima EKANAYAKE ; Chul Min PARK ; Changjong MOON ; Kyungsook JUNG ; Akane TANAKA ; Hiroshi MATSUDA ; Taekyun SHIN
Experimental Neurobiology 2019;28(1):74-84
Olfactory dysfunction occurs in multiple sclerosis in humans, as well as in an animal model of experimental autoimmune encephalomyelitis (EAE). The aim of this study was to analyze differentially expressed genes (DEGs) in olfactory bulb of EAE-affected mice by next generation sequencing, with a particular focus on changes in olfaction-related signals. EAE was induced in C57BL/6 mice following immunization with myelin oligodendrocyte glycoprotein and adjuvant. Inflammatory lesions were identified in the olfactory bulbs as well as in the spinal cord of immunized mice. Analysis of DEGs in the olfactory bulb of EAE-affected mice revealed that 44 genes were upregulated (and which were primarily related to inflammatory mediators), while 519 genes were downregulated; among the latter, olfactory marker protein and stomatin-like 3, which have been linked to olfactory signal transduction, were significantly downregulated (log2 [fold change] >1 and p-value < 0.05). These findings suggest that inflammation in the olfactory bulb of EAE-affected mice is associated with the downregulation of some olfactory signal transduction genes, particularly olfactory marker protein and stomatin-like 3, which may lead to olfactory dysfunction in an animal model of human multiple sclerosis.
Animals
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Down-Regulation
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Encephalomyelitis, Autoimmune, Experimental
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Gene Expression
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Humans
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Immunization
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Inflammation
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Mice
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Models, Animal
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Multiple Sclerosis
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Myelin-Oligodendrocyte Glycoprotein
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Olfactory Bulb
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Olfactory Marker Protein
;
Signal Transduction
;
Spinal Cord
;
Transcriptome
6.Attenuation of Experimental Autoimmune Uveitis in Lewis Rats by Betaine
Yuna CHOI ; Kyungsook JUNG ; Hyo Jin KIM ; Jiyoon CHUN ; Meejung AHN ; Youngheun JEE ; Hyun Ju KO ; Changjong MOON ; Hiroshi MATSUDA ; Akane TANAKA ; Jeongtae KIM ; Taekyun SHIN
Experimental Neurobiology 2021;30(4):308-317
Experimental autoimmune uveitis (EAU) is an animal model of human autoimmune uveitis that is characterized by the infiltration of autoimmune T cells with concurrent increases in pro-inflammatory cytokines and reactive oxygen species. This study aimed to assess whether betaine regulates the progression of EAU in Lewis rats. EAU was induced via immunization with the interphotoreceptor retinoid-binding protein (IRBP) and oral administration of either a vehicle or betaine (100 mg/kg) for 9 consecutive days. Spleens, blood, and retinas were sampled from the experimental rats at the time of sacrifice and used for the T cell proliferation assay, serological analysis, real-time polymerase chain reaction, and immunohistochemistry. The T cell proliferation assay revealed that betaine had little effect on the proliferation of splenic T cells against the IRBP antigen in an in vitro assay on day 9 post-immunization. The serological analysis showed that the level of serum superoxide dismutase increased in the betainetreated group compared with that in the vehicle-treated group. The anti-inflammatory effect of betaine was confirmed by the downregulation of pro-inflammation-related molecules, including vascular cell adhesion molecule 1 and interleukin-1β in the retinas of rats with EAU. The histopathological findings agreed with those of ionized calcium-binding adaptor molecule 1 immunohistochemistry, further verifying that inflammation in the retina and ciliary bodies was significantly suppressed in the betaine-treated group compared with the vehicle-treated group. Results of the present study suggest that betaine is involved in mitigating EAU through anti-oxidation and anti-inflammatory activities.
7.Attenuation of Experimental Autoimmune Uveitis in Lewis Rats by Betaine
Yuna CHOI ; Kyungsook JUNG ; Hyo Jin KIM ; Jiyoon CHUN ; Meejung AHN ; Youngheun JEE ; Hyun Ju KO ; Changjong MOON ; Hiroshi MATSUDA ; Akane TANAKA ; Jeongtae KIM ; Taekyun SHIN
Experimental Neurobiology 2021;30(4):308-317
Experimental autoimmune uveitis (EAU) is an animal model of human autoimmune uveitis that is characterized by the infiltration of autoimmune T cells with concurrent increases in pro-inflammatory cytokines and reactive oxygen species. This study aimed to assess whether betaine regulates the progression of EAU in Lewis rats. EAU was induced via immunization with the interphotoreceptor retinoid-binding protein (IRBP) and oral administration of either a vehicle or betaine (100 mg/kg) for 9 consecutive days. Spleens, blood, and retinas were sampled from the experimental rats at the time of sacrifice and used for the T cell proliferation assay, serological analysis, real-time polymerase chain reaction, and immunohistochemistry. The T cell proliferation assay revealed that betaine had little effect on the proliferation of splenic T cells against the IRBP antigen in an in vitro assay on day 9 post-immunization. The serological analysis showed that the level of serum superoxide dismutase increased in the betainetreated group compared with that in the vehicle-treated group. The anti-inflammatory effect of betaine was confirmed by the downregulation of pro-inflammation-related molecules, including vascular cell adhesion molecule 1 and interleukin-1β in the retinas of rats with EAU. The histopathological findings agreed with those of ionized calcium-binding adaptor molecule 1 immunohistochemistry, further verifying that inflammation in the retina and ciliary bodies was significantly suppressed in the betaine-treated group compared with the vehicle-treated group. Results of the present study suggest that betaine is involved in mitigating EAU through anti-oxidation and anti-inflammatory activities.
8.Histopathological evaluation of the Pathology lungs in experimental autoimmune encephalomyelitis
Sungmoo HONG ; Jeongtae KIM ; Kyungsook JUNG ; Meejung AHN ; Changjong MOON ; Yoshihiro NOMURA ; Hiroshi MATSUDA ; Akane TANAKA ; Hyohoon JEONG ; Taekyun SHIN
Journal of Veterinary Science 2024;25(3):e35-
Objective:
This study evaluated the inflammatory response in lungs of EAE mice by immunohistochemistry and histochemistry.
Methods:
Eight adult C57BL/6 mice were injected with myelin oligodendrocyte glycoprotein 35-55 to induce the EAE. Lungs and spinal cords were sampled from the experimental mice at the time of sacrifice and used for the western blotting, histochemistry, and immunohistochemistry.
Results:
Histopathological examination revealed inflammatory lesions in the lungs of EAE mice, characterized by infiltration of myeloperoxidase (MPO)- and galectin-3-positive cells, as determined by immunohistochemistry. Increased numbers of collagen fibers in the lungs of EAE mice were confirmed by histopathological analysis. Western blotting revealed significantly elevated level of osteopontin (OPN), cluster of differentiation 44 (CD44), MPO and galectin-3 in the lungs of EAE mice compared with normal controls (p < 0.05).Immunohistochemical analysis revealed both OPN and CD44 in ionized calcium-binding adapter molecule 1-positive macrophages within the lungs of EAE mice.
Conclusions
and Relevance: Taken together, these findings suggest that the increased OPN level in lungs of EAE mice led to inflammation; concurrent increases in proinflammatory factors (OPN and galectin-3) caused pulmonary impairment.