1.The Study on the Effects of Psoralen Derivatives on Epidermal Melanocytes in C57 BL Mice after Topical Photochemotherapy.
Seung Min LEE ; Seung Kyung HANN ; Yoon Kee PARK
Annals of Dermatology 1994;6(1):1-8
BACKGROUND: Monofunctional psoralens plus UVA radiation are not severely phototoxic and have less mutagenic activity than bifunctional psoralens plus UVA radiation. OBJECTIVE: The purpose of this study was to evaluate pigment producing effect using various concentrations(0.02%, 0.1%, 0.5%) of monofunctional psoralens such as angelicin, khellin and comparing it's effect with TMP in topical photochemotherapy. METHOD: Ninty three C57BL mice were painted with either angelicin, khellin or TMP solution in concentrations of 0.02%, 0.1% and 0.5% each and were UVA irradiated. Skin biopsies were performed at 1,3,5 weeks after UVA irradiation. The pigment producing effects were measured by the number, area and perimeter of the melanocytes after topical PUVA. RESULTS: The comparison of melanocyte numbers between different psoralens after five weeks of photochemotherapy showed a significant difference in decreasing order of TMP, khellin and angelicin. The area and perimeter of melanocytes were larger in the TMP group after five weeks photochemotherapy than the other group. However in the khellin and angelicin group, the area and perimeter of melanocytes were not increased by increasing the frequency of the UVA irradiation. CONCLUSION: The number, area and perimeter of melanocytes after topical PUVA increased in the TMP group compared to angelicin or khellin group. We expect the clinical application of angelicin and khellin in vitiligo is possible considering the result of the study of pigment producing effect with a higher concentration and higher dose of UVA.
Animals
;
Biopsy
;
Ficusin*
;
Furocoumarins
;
Khellin
;
Melanocytes*
;
Methods
;
Mice*
;
Mice, Inbred C57BL
;
Paint
;
Photochemotherapy*
;
Skin
;
Thymidine Monophosphate
;
Vitiligo
2.Morphological and Biomechanical Stidy about the Avascular Necrosis of the Femoral Head Part I : Morphological Classification and Treatment Modality
Young Min KIM ; Kyung Hoi KOO ; Kang Sup YOON
The Journal of the Korean Orthopaedic Association 1990;25(2):477-486
The exact etiologies and mechanisms of ischemia in avascular necrosis of femoral head are still under investigation. But this condition seems to be increasing during a few decades with increasing use of steroid and alcohol abuse in Korea. The treatment modalities have been based on the stages of the disease by simple radiographic findings that do not describe the accurate pathological extent or localization. Therefore more sophisticated study is needed to treat the AVN of the femoral head well. Magnetic resonance images, simple radiographs, scintigraphs of 13 patients(22 femoral heads), who were admitted and treated at Seoul National University Hospital from April, 1989 to December, 1989 were correlated, to characterize the type of AVN by extent and location regardless of its stage and reveal the relationship between types and stages. It is the result of this study that the type of AVN as well as stage should be considered in determining the treatment modality.
Alcoholism
;
Classification
;
Head
;
Ischemia
;
Korea
;
Magnetic Resonance Imaging
;
Necrosis
;
Seoul
3.The Change in Regional Cerebral Oxygen Saturation after Stellate Ganglion Block.
Hyeon Min PARK ; Tae Wan KIM ; Hong Gyu CHOI ; Kyung Bong YOON ; Duck Mi YOON
The Korean Journal of Pain 2010;23(2):142-146
BACKGROUND: Stellate ganglion block (SGB) is known to increase blood flow to the innervations area of the stellate ganglion. Near infrared spectroscopy reflects an increased blood volume and allows continuous, non-invasive, and bedside monitoring of regional cerebral oxygen saturation (rSO2). We investigated the influence of SGB on bilateral cerebral oxygenation using a near infrared spectroscopy. METHODS: SGB was performed on 30 patients with 1% lidocaine 10 ml using a paratracheal technique at the C6 level and confirmed by the presence of Horner's syndrome. The blood pressure (BP), heart rate (HR) and rSO2 were measured before SGB and 5, 10, 15 and 20 minutes after SGB. Tympanic temperature of each ear was measured prior to SGB and 20 minutes after SGB. RESULTS: The increments of the rSO2 on the block side from the baseline were statistically significant at 5, 10, 15 and 20 minutes. The rSO2 on the non-block side compared with the baseline, however, decreased at 15 and 20 minutes. The difference between the block and the non-block sides was significant at 15 and 20 minutes. The BP at 10, 15 and 20 minutes was increased and the HR was increased at 10 and 15 minutes. CONCLUSIONS: We observed an increment of the rSO2 on the block side from the baseline; however, the rSO2 on the non-block side decreased.
Blood Pressure
;
Blood Volume
;
Ear
;
Heart Rate
;
Horner Syndrome
;
Humans
;
Lidocaine
;
Oxygen
;
Spectrum Analysis
;
Stellate Ganglion
4.The activity of antimelanocyte autoantibodies in vitiligo patients.
Yoon Kee PARK ; Seung Kyung HANN ; Min Seok SONG ; Jung Ku YOON ; Hyung Il KIM
Korean Journal of Dermatology 1991;29(3):391-398
Several observation suggest that the antimelanocyte autoantibodies could play a role in melanocyte destruction. Some experiments indicate that melanocyte antibodies from patients with vitiligo can kill melanocyte in vitro. In these experiments, we demonstrated that vitiligo patient's sera containing antimelanocyte antibodies can lyse cultured human melanocytes by complement activation. Melanocyte cytotoxicity was measured using the ethidium bromide/ acridine orange viability assay. Significant melanocyte cytotoxicity was seen in sera from patients with both active and inactive vitiligo(p<0.01). Melanocyte cytotoxicity measured with complement-mediated cytotoxicity decreased after systemic steroid treatment(p<0.05) ; however melanocyte cytotoxicity showed no significant change with systemic PUVA therapy.
Acridine Orange
;
Antibodies
;
Autoantibodies*
;
Complement Activation
;
Ethidium
;
Humans
;
Melanocytes
;
PUVA Therapy
;
Vitiligo*
5.Neonatal Congenital Fibrosarcoma: A Case Report
Hyung Ku YOON ; Kuk Hwan OH ; Kyung Hoon KANG ; Kwang Pyo JEON ; Dae Eun JUNG ; Min Su YOON
The Journal of the Korean Orthopaedic Association 1994;29(5):1357-1361
Congenital fibrosarcoma of neonate is a very rare malignant tumor prone to behave aggressively with a tendency to local recurrence without metastasis. It is characteristically composed of collagenous fibroblasts and herringbone pattern in histology. We experienced a case of congenital fibrosarcoma on the left thigh and report this case with some review in literature with 1½ year follow up.
Collagen
;
Fibroblasts
;
Fibrosarcoma
;
Follow-Up Studies
;
Humans
;
Infant, Newborn
;
Neoplasm Metastasis
;
Recurrence
;
Thigh
6.Incidence and Contributing Factors of Malpositioning of the Endotracheal Tube after Endotracheal Intubation with Chest X-ray.
Kyoung Seop YOON ; Kyung Min LEE ; Hyun Kyo LIM ; Dae Ja UM
Korean Journal of Anesthesiology 1997;33(1):98-103
BACKGROUND: Malpositioning of the endotracheal tube within airway after intubation may results in serious complications, such as accidental extubation and inadvertent endobronchial intubation. Therefore, early detection of malposioning of the endotracheal tube is very impotant for deciding patient's prognosis. METHODS: We assessed the distance from the tip of the endotracheal tube to the carina according to the patient's age, sex, and the time, the location, and the route of intubation. The chest x-ray was taken for 333 patients (men, 226: women, 107) who were admitted to the intensive care unit of Wonju Christian Hospital from march 1, 1995 to February 28, 1996. Chest x-ray was obtained after intubation to verify endotracheal tube position. Appropriate endotracheal tube position on chest x-ray was defined as between 2 and 6 cm above the carina. RESULTS: Of the 333 intubations, 106 (31.8%) endotracheal tubes were inappropriately placed according to the chest x-ray. The percentage of malpositioned endotracheal tubes (<2 cm) was higher in women than in men (11.2% vs. 5.3%, p<0.05), with higher in night (7 PM to 7 AM) than in day (7 AM to 7 PM) (42% vs. 24%, p<0.05). Distance from the carina to the tip of endotracheal tube is 4.34 +/- 1.77cm in women and 5.23 +/- 1.64cm in men. Thus, position of the endotracheal tube in women is deeper than men (p<0.001). CONCLUSIONS: We conclude that the chest x-ray for confirmation of endotracheal tube position after endotracheal intubation may remain the standard of practice. And endotracheal tube position should be carefully assessed immediately after tracheal intubation, particularly in women and at night.
Female
;
Gangwon-do
;
Humans
;
Incidence*
;
Intensive Care Units
;
Intubation
;
Intubation, Intratracheal*
;
Male
;
Prognosis
;
Thorax*
7.Endoscopic Variceal Ligation for Treatment of Esophageal Varices.
Joon Soo HAHM ; Choon Suhk KEE ; Kyung Nam PARK ; Min Ho LEE ; Seok Jin YOON
Korean Journal of Gastrointestinal Endoscopy 1994;14(3):325-330
Endoscopic injection sclerotherapy(EIS) has been widely used in treating and eradicating acutely bleeding esophageal varies, but may be associated with some undesirable local and systemic complications. Endoscopic variceal ligation(EVL), which consists of mechanicai ligation and thrombosis of varices using elastic o-band, has been recently developed as a non operative alternative to EIS. We performed EVL in 65 patients who had bled from esophageal varices between November 1991 and September 1993. Total 274 sessions were performed and 774 o-bands were used. Six patients were actively bleeding and all of them were successfully controlled by emergency EVL. During the follow-up period, five patients who had combined hepatoma died. Varices were eradicated or reduced grade I in 43(71.6%) of the 60 survivals by 8-36 ligations(mean 15.6 ligation) in 2-13 EVL sessions(mean 5.6 sessions). During follow up period, five patients had recurred from grade 0 to grade 2 or 3 in 106-260 days(mean 182.6 days), and then eradicated by repeated EVL. During or after EVL, there were no complications, except mild substernal distress and mild dysphagia in 17 and 7 patients respectively. These results showed that EVL is a safe and effective method for eradication of bleeding esophageal varices.
Carcinoma, Hepatocellular
;
Deglutition Disorders
;
Emergencies
;
Esophageal and Gastric Varices*
;
Follow-Up Studies
;
Hemorrhage
;
Humans
;
Ligation*
;
Thrombosis
;
Varicose Veins
8.A case of occupational asthma induced by latexin a hospital personnel.
Byung Jae LEE ; Yoon Keun KIM ; Kyung Up MIN ; You Young KIM
Journal of Asthma, Allergy and Clinical Immunology 1998;18(3):510-517
IgE-mediated sensitization to natural rubber latex can induce immediate hypersensitivity reactions ranging from contact urticaria to life threatening anaphylaxis. Recent reports suggest that asthma is also relatively frequent manifestation of latex allergy. In this case report, lat,ex induced asthma is described in an operat.ing room nurse regularly exposed t,o latex gloves. Her latex sensitivity was detected by skin prick testing. Specific bronchial provocation test with latex extract showed an early asthmatic reaction. Her symptoms had been much improved after avoidance. Occupational asthma induced by latex may be not uncommon among health care workers.
Anaphylaxis
;
Asthma
;
Asthma, Occupational*
;
Bronchial Provocation Tests
;
Delivery of Health Care
;
Humans
;
Hypersensitivity, Immediate
;
Latex
;
Latex Hypersensitivity
;
Personnel, Hospital*
;
Rubber
;
Skin
;
Urticaria
9.A Case of Central Nervous System Toxicity Assoclated with Cyclosporine.
Do Yoon LEE ; Mee Kyung NAMGOONG ; Hwang Min KIM ; Baek Keum LIM
Journal of the Korean Society of Pediatric Nephrology 1997;1(2):179-182
Benign and malignant papillary neoplasms of the breast may be difficult to distinguish in both cytologic and histologic preparations. To define the cytologic features of benign and malignant papillary lesions, we retrospectively reviewed 18 cases of fine needle aspirates from histologically confirmed cases of papilloma or papillary carcinoma of the breast. This study included 3 intraductal papillary carcinomas, 3 invasive papillary carcinomas, and 12 intraductal papillomas. All cases were evaluated for presence or absence of papillary fragments, bloody background, apocrine metaplasia, macrophages, and degree of cellularity, atypia, and single isolated columnar epithelial cells. Papillary fragments were present in all cases. The background of the smear was bloody in all 6 carcinomas, but in only 7 out of 12 papillomas. Markedly increased cellularity was present in 4 carcinomas (67%) and 7 papillomas (58%). Single cells were present in 5 carcinomas (83%) and 8 papillomas (67%). The majority of papillomas and papillary carcinomas had mild to moderate atypia, and severe atypia was noted in one case of intraductal papillary carcinoma and one case of invasive papillary carcinoma. Apocrine metaplasia was absent in all cases of papillary carcinomas, but present in 8 papillomas (67%). Macrophages were noted in 4 carcinomas and were present in all cases of papillomas. The constellation of severe atypia, bloody background, absence of apocrine metaplasia and/or macrophages were features to favor carcinoma. Malignant lesions tended to show higher cellularity and more single isolated cells. The cytologic features mentioned above would be helpful to distinguish benign from malignant papillary lesions of the breast. However, because of overlapping of cytologic features, surgical excision should be warranted in all cases of papillary lesions of the breast to further characterize the tumor
Breast
;
Carcinoma, Papillary
;
Central Nervous System*
;
Cyclosporine*
;
Epithelial Cells
;
Macrophages
;
Metaplasia
;
Needles
;
Papilloma
;
Papilloma, Intraductal
;
Retrospective Studies
10.Childhood Viltiligo.
Seung Kyung HANN ; Min Seok SONG ; Yoon Kee PARK ; Sung Ku AHN
Annals of Dermatology 1991;3(2):112-118
We investigated the clinical aspect and immunologic parameters of childhood and adult vitiligo in our clinics and made a comparative study. The childhood vitiligo showed the following results : similar incidence of clinical patterns with adults except for the lower incidence of the acrofacial type than in adults ; less involvement in the extent of lesion of disease than in adults ; higher incidence of family history than with adults ; of the precipitating factors, trauma is the main one whereas emotional stress is for adults ; higher association with halo nevi than adults ; and lower incidence of serum autoantibodies than adults. We have ascertained that childhood vitiligo is a distinct subset of vitiligo, showing the above features which will be studied in more patients in a long term follow-up clinical study.
Adult
;
Autoantibodies
;
Clinical Study
;
Follow-Up Studies
;
Humans
;
Incidence
;
Nevus, Halo
;
Precipitating Factors
;
Stress, Psychological
;
Vitiligo