Proliferative vitreoretinopathy (PVR) is a main cause of failure in retinal reattachment surgery. There have been many studies about the inhibition of proliferative vitreoretinophthy with several drugs. Authors investigated the inhibitory effect of proliferative vitreoretinopathy and retinal toxicity with various concentration of daunorubicin after intravitreal injection into the eyes of the pigmented rabbit. 7 pigment rabbit (11eyes) were used as subjects. After lensectomy and vitrectomy, control group was injected dermal fibroblast and F-BSS, and treatment group was injected dermal fibroblast and 5, 10, 15, 30 nmol Daunorubicin. At two weeks after intravitreal injection, both group were enucleated and examined with gross finding, light--microscopy, and electronmicroscopy. In all control group, proliferative vitreoretinopathy was found, but only preretinal membrane formation was found in 5, 10 nmol Daunorubicin injected group. In 15 nmol Daunorubicin injected group, the retina structure was preserved normally. In 30 nmol Daunorubicin injected group, the retinal outer segment was degenerated in microscopic finding. These results show that Daunorubicin has a potent effect on proliferative vitreoretinopathy, especially in 15 nmol, but retinal toxicity is suspected in marethan 30 nmol.
Daunorubicin* ; Fibroblasts ; Intravitreal Injections ; Membranes ; Retina ; Retinal Photoreceptor Cell Outer Segment ; Retinaldehyde ; Vitrectomy ; Vitreoretinopathy, Proliferative*

Pattern-reversal retinal potentials(PRRP) are electrical signals generated within retina, possibly by the retinal ganglion cells, when a phase-alternating check board pattern is viewed. Authors clinically studied the characteristics of PRRP the mean amplitude and latency with 24 minute checks, the effect of the spatial frequency, the effect of defocusing and the retinocortical time in 20 normals, using Nicolet CA 1,000. The results are as follows; 1. The mean latency P1 and the mean P1-N2 amplitude of PRRP in normal group was 39.19 +/- 3.30(msec), 1.32 +/- 0.22(uV), respectively. 2. The mean retinocortical time in normal group was 52.93 +/- 7.39(msec). 3. The P1-N2 amplitude of PRRP was reduced linearly with increasing defocusing, and significant amplitude reduction was observed when defocusing amounted to +1D. 4. When central 3 degree of stimulus was covered in order to simulate a macular pathology, PRRP to 24 minute checks was abnormal both in amplitude and latency. 5. Peak response amplitude of PRRP was obtained with large checksizes(3 degrees 12 minutes, 6 degrees 24 minutes).
Pathology ; Retina ; Retinal Ganglion Cells ; Retinaldehyde*

The pathogenesis of IgA nephropathy is unknown, but the systemic character of the IgA deposits (skin and glomerular capillaries), the presence of circulating IgG and IgA complexes and its similarity to Henoch-Schonlein purpura suggest that it is an immune-complex mediated disease. The nature and source of the antigen are unknown. Anatomically, the extremely vascular uvea offers a favorable site for the interplay of various components of immune reaction. We have experienced a 21-year old male who had a choroiditis and IgA nephropathy and had suffered the Henoch-Schonlein purpura when he was 16-years old. We performed the choroidal aspiration and scleral buckling, but steroid and other specific therapy were not given. In the course of follow-up check, the choroidal lesion and hematuria were progressively subsided. In conclusion, we report that the choroiditis is manifested as a part of immunecomplex mediated disease associated with IgA nephropathy.
Adolescent ; Choroid* ; Choroiditis* ; Follow-Up Studies ; Glomerulonephritis, IGA* ; Hematuria ; Humans ; Immunoglobulin A* ; Immunoglobulin G ; Male ; Purpura, Schoenlein-Henoch ; Scleral Buckling ; Uvea ; Young Adult

BACKGROUND: Induction of anesthesia with a high dose of fentanyl and vecuronium decreases the heart rate and blood pressure. This study was designed to evaluate the effect of preinduction atropine on these hemodynamic changes in patients undergoing coronary artery bypass graft surgery (CABG). METHODS: Forty-one patients who underwent CABG were randomly divided into two groups. After insertion of a radial artery cannula and a Swan-Ganz catheter, normal saline 1 ml (control group, n = 20) or atropine 0.5 mg (atropine group, n = 21) was injected intravenously 1 min before the induction of anesthesia. Anesthesia was induced with a first dose of fentanyl (5-8 microgram/kg) and vecuronium (0.12 mg/kg) and a second dose of fentanyl (5-10 microgram/kg). The patient was then intubated. Hemodynamic variables were measured before the induction of anesthesia, 1 min after the administration of each drug during the induction of anesthesia and 5, 10, and 30 min after the intubation. RESULTS: There was no significant differences between the two groups in terms of demographic data except that the number of patients with diabetes mellitus was greater in the control group than in the atropine group. The number of patients treated for hypotension or bradycardia during the induction of anesthesia was greater in the control group than in the atropine group, but this was not statistically significant. Heart rates significantly decreased in the control group but were maintained in the atropine group without any significant tachycardia. Blood pressure significantly decreased in both groups. CONCLUSIONS: Intravenous injection of atropine before anesthetic induction in patients undergoing CABG attenuates the decrease in heart rate resulting from anesthetic induction with high dose fentanyl and vecuronium. However, it didn't prevent the decrease in blood pressure nor did it reduce the incidence of treatment for hypotension.
Anesthesia ; Atropine* ; Blood Pressure ; Bradycardia ; Catheters ; Coronary Artery Bypass* ; Coronary Vessels* ; Diabetes Mellitus ; Fentanyl* ; Heart Rate ; Hemodynamics* ; Humans ; Hypotension ; Incidence ; Injections, Intravenous ; Intubation ; Radial Artery ; Tachycardia ; Transplants ; Vecuronium Bromide*

The acute retinal necrosis is characterized by necrotizing retinitis, vitritis, and retinal vasculitis occurring in otherwise healthy patients. It develops into retinal necrosis and detachment. The clinical course of ARN is rapidly progressive despite therapeutic efforts. We experienced 6 eyes of 5 cases with ARN. In this study, the distribution of patients was 3 males and 2 females and they were from 23 to 53 years old in age, average 39.4 years. The duration of observation was from 3 days to 24 months and we observed retinal detachment in 2 cases(40%) that developed 2 weeks and 4 weeks after onset in each cases. One case involved both eyes, and the other, in one eye. Four of 6 eyes(65%) had a final visual acuity of less than 0.1.
Female ; Humans ; Male ; Middle Aged ; Necrosis ; Retinal Detachment ; Retinal Necrosis Syndrome, Acute* ; Retinal Vasculitis ; Retinaldehyde ; Retinitis ; Visual Acuity

Authority estimated the concentration of fluorescein sodium in the blood, anterior chamber and vitreous after injection of 10% fluorescein sodium, 25mg/kg, according to the lapse of time. Also intravitreal fluorescein sodium concentration was measured after intravitreal injection of 0.1 % fluorescein sodium 10 micro liter according to the lapse of time. The results were as follows. 1. In the normal rabbits, the concentration of fluorescein sodium in the anterior chamber and vitreous were 159.3 +/- 101.7 X 10(-9) gm/ml and 3.6 +/- 4.7 X 10(-9) gm/ml, respectively after 1 hour of fluorescein sodium intravenous injection. The Cv/Ca was 0.02 +/- 0.028(Cv: concentration of fluorescein in the vitreous) (Ca: concentration of fluorescein in the ant. chamber). 2. After injection of sodium fluorescein in the normal rabbit, the concentration of fluorescein in the blood showed rapid decrease in logarithmic manner in the vitreous, the concentration peaked 1 hour and 2 hours after injection which persisted several hours. 3. The concentration of fluorescein sodium in the vitreous after intravitreal injection of fluorescein decreased at logarithmic rate which disappeared 24 hours after injection.
Anterior Chamber ; Ants ; Fluorescein* ; Injections, Intravenous ; Intravitreal Injections ; Pharmacokinetics* ; Rabbits

In order to evaluate the components of the proliferative membranes in the retinopathy of prematurity (ROP), 8 proliferative membranes obtained from 8 eyes with ROP stage 5 during lensectomy, vitrectomy and delamination of membrane were examined under electron mIcroscopy. Among the 8 proliferative membranes of ROP, fibrous astrocytes, myofibroblasts, lymphocytes, macrophages, calcifications were respectively found in 2 cases, vascular tissues in 4 cases and abundant collagen matrix in 7 cases. While in the 2 cases with fibrous astrocytic proliferations the membranes were hypercellular, in the other 6 cases the membranes were hypocellular and mainly composed of collagen matrix.
Astrocytes ; Collagen ; Lymphocytes ; Macrophages ; Membranes* ; Microscopy, Electron ; Myofibroblasts ; Retinopathy of Prematurity* ; Vitrectomy

We have attempted to measure parafoveal retinal acuity directly on the exact retinal locus, while observing the retinal image in real time using the scanning laser ophthalmoscope(SLO 101, Rodenstock, Munish, Germany). By the SLO Visumetry software(Rodenstock v. 3.0), thirty eyes of healthy volunteers were examined in 20degrees image field. Using Snellen E as stimulus, the examination was performed from the fovea by the radial pattern. The maximal retinal distance point, which responded to stimulus, was recorded by the pixel, and the distance(mm) from the fovea was calculated by the Bennett formula. The maximum distance from the fovea at the given stimulus size was achieved as follows: 0.32+/-0.01mmat the 15 x15 arc of minute(0.333), 0.63+/-0.01mm at the 17 x17 arc of minute(0.294), 1.05+/-0.03 mmat 20 x 20 arc of minute(0.25), and 1.44+/-0.0 5 mmat the 23 x23 arc of minute(0.217). It was also revealed that the horizontal maximal distance from fovea at given stimulus size was statistically superior to the vertical maximal distance(p<0.05). In conclusion we were able to establish the normal range of parafoveal retinal acuity in healthy volunteers. It may serve as the baseline for subsequent study of retinal pathology and functional evaluation as well as its treatment.
Healthy Volunteers* ; Pathology ; Reference Values ; Retinaldehyde* ; Visual Acuity*

In the condition of diabetic retinopathy, the vascular changes are localized primarily in the retinal capillaries and are presumed to promote angiogenesis. To investigate the change of retinal blood flow velocities and morphological parameters in diabetic retinopathy, we measured perifoveal capillary blood velocities(v) and the size of foveal avascular zones(FAZ). Thirteen patients with diabetic maculopathy and nine healthy volunteers were included in this study. The scanning laser technique in conjunction with an image analysing system were used to assess the morphological and hemodynamic changes in diabetic retinopathy. Diabetic maculopathy group showed a slower capillary blood velocity than normal group(2.44+/-0.39mm/sec vs2.75+/-0.61 mm/sec, p>0.18). The foveal avascular zone was significantly larger in diabetic maculopathy group than in normal group(313.5+/-64.6micrometervs. 238.9+/-93.8micrometer, p<0.05). This results indicate that the retinal microcirculation is altered in diabetic patients compared with healthy subjects. These alterations may be due to the change of the capillary wall and blood viscosity in diabetic patients. The determination of these parameters can be utilized in monitoring the progress of diabetic maculopathy.
Blood Flow Velocity ; Blood Viscosity ; Capillaries ; Diabetic Retinopathy ; Healthy Volunteers ; Hemodynamics ; Humans ; Microcirculation ; Retinaldehyde

Electrical conductivity of eyeball replaced with vitreous substitutes were also measured in both the non-enucleated and enucleated eye. The study of correlation between the electroretinogram and conductivity of eyeball to be replaced with vitreous substitutes was also evaluated. The electrical conductivity of each vitreous replacement was shown 74.5% in vitreous, 77.5% in saline, 100% in air and silicone oil respectively relative to standard material -1.5 battery, 100%. There was no difference of conductivity between the enucleated eye and non-enucleated eye. There were no correlation between the ERG amplitude and conductivity. As a result of experiments, the decrease in amplitude is suggested as a damage of retina by manipulation and surgery rather than decrease of conductivity with vitreous replacements.
Electric Conductivity* ; Rabbits* ; Retina ; Silicone Oils