Proliferative vitreoretinopathy (PVR) is a main cause of failure in retinal reattachment surgery. There have been many studies about the inhibition of proliferative vitreoretinophthy with several drugs. Authors investigated the inhibitory effect of proliferative vitreoretinopathy and retinal toxicity with various concentration of daunorubicin after intravitreal injection into the eyes of the pigmented rabbit. 7 pigment rabbit (11eyes) were used as subjects. After lensectomy and vitrectomy, control group was injected dermal fibroblast and F-BSS, and treatment group was injected dermal fibroblast and 5, 10, 15, 30 nmol Daunorubicin. At two weeks after intravitreal injection, both group were enucleated and examined with gross finding, light--microscopy, and electronmicroscopy. In all control group, proliferative vitreoretinopathy was found, but only preretinal membrane formation was found in 5, 10 nmol Daunorubicin injected group. In 15 nmol Daunorubicin injected group, the retina structure was preserved normally. In 30 nmol Daunorubicin injected group, the retinal outer segment was degenerated in microscopic finding. These results show that Daunorubicin has a potent effect on proliferative vitreoretinopathy, especially in 15 nmol, but retinal toxicity is suspected in marethan 30 nmol.
Daunorubicin* ; Fibroblasts ; Intravitreal Injections ; Membranes ; Retina ; Retinal Photoreceptor Cell Outer Segment ; Retinaldehyde ; Vitrectomy ; Vitreoretinopathy, Proliferative*

Pattern-reversal retinal potentials(PRRP) are electrical signals generated within retina, possibly by the retinal ganglion cells, when a phase-alternating check board pattern is viewed. Authors clinically studied the characteristics of PRRP the mean amplitude and latency with 24 minute checks, the effect of the spatial frequency, the effect of defocusing and the retinocortical time in 20 normals, using Nicolet CA 1,000. The results are as follows; 1. The mean latency P1 and the mean P1-N2 amplitude of PRRP in normal group was 39.19 +/- 3.30(msec), 1.32 +/- 0.22(uV), respectively. 2. The mean retinocortical time in normal group was 52.93 +/- 7.39(msec). 3. The P1-N2 amplitude of PRRP was reduced linearly with increasing defocusing, and significant amplitude reduction was observed when defocusing amounted to +1D. 4. When central 3 degree of stimulus was covered in order to simulate a macular pathology, PRRP to 24 minute checks was abnormal both in amplitude and latency. 5. Peak response amplitude of PRRP was obtained with large checksizes(3 degrees 12 minutes, 6 degrees 24 minutes).
Pathology ; Retina ; Retinal Ganglion Cells ; Retinaldehyde*

The pathogenesis of IgA nephropathy is unknown, but the systemic character of the IgA deposits (skin and glomerular capillaries), the presence of circulating IgG and IgA complexes and its similarity to Henoch-Schonlein purpura suggest that it is an immune-complex mediated disease. The nature and source of the antigen are unknown. Anatomically, the extremely vascular uvea offers a favorable site for the interplay of various components of immune reaction. We have experienced a 21-year old male who had a choroiditis and IgA nephropathy and had suffered the Henoch-Schonlein purpura when he was 16-years old. We performed the choroidal aspiration and scleral buckling, but steroid and other specific therapy were not given. In the course of follow-up check, the choroidal lesion and hematuria were progressively subsided. In conclusion, we report that the choroiditis is manifested as a part of immunecomplex mediated disease associated with IgA nephropathy.
Adolescent ; Choroid* ; Choroiditis* ; Follow-Up Studies ; Glomerulonephritis, IGA* ; Hematuria ; Humans ; Immunoglobulin A* ; Immunoglobulin G ; Male ; Purpura, Schoenlein-Henoch ; Scleral Buckling ; Uvea ; Young Adult

BACKGROUND: Induction of anesthesia with a high dose of fentanyl and vecuronium decreases the heart rate and blood pressure. This study was designed to evaluate the effect of preinduction atropine on these hemodynamic changes in patients undergoing coronary artery bypass graft surgery (CABG). METHODS: Forty-one patients who underwent CABG were randomly divided into two groups. After insertion of a radial artery cannula and a Swan-Ganz catheter, normal saline 1 ml (control group, n = 20) or atropine 0.5 mg (atropine group, n = 21) was injected intravenously 1 min before the induction of anesthesia. Anesthesia was induced with a first dose of fentanyl (5-8 microgram/kg) and vecuronium (0.12 mg/kg) and a second dose of fentanyl (5-10 microgram/kg). The patient was then intubated. Hemodynamic variables were measured before the induction of anesthesia, 1 min after the administration of each drug during the induction of anesthesia and 5, 10, and 30 min after the intubation. RESULTS: There was no significant differences between the two groups in terms of demographic data except that the number of patients with diabetes mellitus was greater in the control group than in the atropine group. The number of patients treated for hypotension or bradycardia during the induction of anesthesia was greater in the control group than in the atropine group, but this was not statistically significant. Heart rates significantly decreased in the control group but were maintained in the atropine group without any significant tachycardia. Blood pressure significantly decreased in both groups. CONCLUSIONS: Intravenous injection of atropine before anesthetic induction in patients undergoing CABG attenuates the decrease in heart rate resulting from anesthetic induction with high dose fentanyl and vecuronium. However, it didn't prevent the decrease in blood pressure nor did it reduce the incidence of treatment for hypotension.
Anesthesia ; Atropine* ; Blood Pressure ; Bradycardia ; Catheters ; Coronary Artery Bypass* ; Coronary Vessels* ; Diabetes Mellitus ; Fentanyl* ; Heart Rate ; Hemodynamics* ; Humans ; Hypotension ; Incidence ; Injections, Intravenous ; Intubation ; Radial Artery ; Tachycardia ; Transplants ; Vecuronium Bromide*

Fundus perimetry is the device that provides visualization of the fundus and the precise localization of the stimuli on it. With the increasing importance of the direct fundus perimetry for certain focal retinal morphologic abnormalities, the scanning laser ophthalmoscope[SLO]has been utilized as a system to eliminate some of the classic technical problems in fundus perimetry. The aim of this study was to evaluate the normal light sensitivity values for microperimetryusing SLO system. Thirty eyes of thirty normal subjects were examined in 60 degrees image field using stimuli of variable intensity. The maximal retinal distance point which responded to stimulus was recorded, point which responded to stimulus was recorded, and the distance[millimeter]from the fovea was calculated by the Bennett formula. The maximum distance from the fovea at the given stimulus intensity was measured as follows :0.7596 +/-0.5262millimeter at 28decibel, 2.2050 +/-0.5878millimeter at 26decibel, 3.0571 +/-0.7151millimeter at 24decibel, 4.3690 +/-1.0973millimeter at 22decibel, and 5.6557 +/-1.3458millimeter at 20decibel. In conclusion, we were able to establish the normal range of light sensitivity in Microperimery examination. It may serve as the baseline for subsequent study of retinal pathology and functional evaluation as well as its treatment.
Ophthalmoscopes* ; Pathology ; Photophobia ; Reference Values* ; Retinaldehyde ; Visual Field Tests

In order to evaluate the components of the proliferative membranes in the retinopathy of prematurity (ROP), 8 proliferative membranes obtained from 8 eyes with ROP stage 5 during lensectomy, vitrectomy and delamination of membrane were examined under electron mIcroscopy. Among the 8 proliferative membranes of ROP, fibrous astrocytes, myofibroblasts, lymphocytes, macrophages, calcifications were respectively found in 2 cases, vascular tissues in 4 cases and abundant collagen matrix in 7 cases. While in the 2 cases with fibrous astrocytic proliferations the membranes were hypercellular, in the other 6 cases the membranes were hypocellular and mainly composed of collagen matrix.
Astrocytes ; Collagen ; Lymphocytes ; Macrophages ; Membranes* ; Microscopy, Electron ; Myofibroblasts ; Retinopathy of Prematurity* ; Vitrectomy

Authority estimated the concentration of fluorescein sodium in the blood, anterior chamber and vitreous after injection of 10% fluorescein sodium, 25mg/kg, according to the lapse of time. Also intravitreal fluorescein sodium concentration was measured after intravitreal injection of 0.1 % fluorescein sodium 10 micro liter according to the lapse of time. The results were as follows. 1. In the normal rabbits, the concentration of fluorescein sodium in the anterior chamber and vitreous were 159.3 +/- 101.7 X 10(-9) gm/ml and 3.6 +/- 4.7 X 10(-9) gm/ml, respectively after 1 hour of fluorescein sodium intravenous injection. The Cv/Ca was 0.02 +/- 0.028(Cv: concentration of fluorescein in the vitreous) (Ca: concentration of fluorescein in the ant. chamber). 2. After injection of sodium fluorescein in the normal rabbit, the concentration of fluorescein in the blood showed rapid decrease in logarithmic manner in the vitreous, the concentration peaked 1 hour and 2 hours after injection which persisted several hours. 3. The concentration of fluorescein sodium in the vitreous after intravitreal injection of fluorescein decreased at logarithmic rate which disappeared 24 hours after injection.
Anterior Chamber ; Ants ; Fluorescein* ; Injections, Intravenous ; Intravitreal Injections ; Pharmacokinetics* ; Rabbits

The acute retinal necrosis is characterized by necrotizing retinitis, vitritis, and retinal vasculitis occurring in otherwise healthy patients. It develops into retinal necrosis and detachment. The clinical course of ARN is rapidly progressive despite therapeutic efforts. We experienced 6 eyes of 5 cases with ARN. In this study, the distribution of patients was 3 males and 2 females and they were from 23 to 53 years old in age, average 39.4 years. The duration of observation was from 3 days to 24 months and we observed retinal detachment in 2 cases(40%) that developed 2 weeks and 4 weeks after onset in each cases. One case involved both eyes, and the other, in one eye. Four of 6 eyes(65%) had a final visual acuity of less than 0.1.
Female ; Humans ; Male ; Middle Aged ; Necrosis ; Retinal Detachment ; Retinal Necrosis Syndrome, Acute* ; Retinal Vasculitis ; Retinaldehyde ; Retinitis ; Visual Acuity

The measurement of the rate of flow of aqueous humor is very important for the investigation of the physiology in the aqueous humor and maintenance of the intraocular pressure. Argon laser trabeculoplasty apears to be effective in lowering intraocular pressure with much advantage over standard filtering operation but despite the, widespread use of argon laser traheculoplasty in medically unresponsive open angle glaucoma, the exact mechanism by which it reduce outflow resistance remains unknown and so the study of the mechanism and the most suitable site are required due to the associated complication and less effectiveness during long term evaluation. We studied the changes in the rate of constant, the rate of flow for 2 weeks, intraocular pressure and C-value for 4 weeks, histopathologic changes for 36 days after argon laser trabeculoplasty in 25 rabbits(21 white and 4 black). The normal control group of 20 rabbits was also measured for intraocular pressure and C-value and the rate of constant, the rate of flow. In trabeculoplasty, we used the Britt 900 argon laser photocoagulator(pulse wave) with 50~60 burns at a setting of 700~800 mW, 0.2 seconds duration, 50 micro beam diameter in 360 degrees portion at the site between scleral spur and trabecularmeshwork. The results were as follows; 1) In control group of 20 rabbits. 1. The mean normal intraocular pressure was 18.5 +/- 2.0mmHg. 2. The mean normal C-value was 0.385 +/- 0.072 cumm/min/mmHg. 3. The mean normal rate of constant was 0.0114 +/- 0.0021min(-1). 4. The mean normal rate of flow was 3.980 +/- 0.735 micro l/min. 5. The relationship between rate of flow and C-value was high. 2) In rabbits after argon laser trabeculoplasty. 1. The white and black rabbits showed a statistically significant decrease of intraocular pressure for 4 weeks and elevation of rate of flow for 2 weeks after argon laser trabeculoplasty (p<0.001). 2. The changes of post-laser intraocular pressure was related with changes of C-value after argon laser trabeculoplasty for 4 weeks. 3. The changes of the mean IOP and C-value for post-laser 4 weeks showed no significant differences between white group and black group. 4. In histopathologic changes for 36 days, inflammtory change was not seen for post-laser period, fibrosis and edema in trabecular meshwork was more prominent at the 8th day, edema and fibrosis was regressed as normal at the post-laser 27th day.
Aqueous Humor* ; Argon* ; Burns ; Edema ; Fibrosis ; Glaucoma, Open-Angle ; Intraocular Pressure ; Physiology ; Rabbits* ; Trabecular Meshwork ; Trabeculectomy*

We have attempted to measure parafoveal retinal acuity directly on the exact retinal locus, while observing the retinal image in real time using the scanning laser ophthalmoscope(SLO 101, Rodenstock, Munish, Germany). By the SLO Visumetry software(Rodenstock v. 3.0), thirty eyes of healthy volunteers were examined in 20degrees image field. Using Snellen E as stimulus, the examination was performed from the fovea by the radial pattern. The maximal retinal distance point, which responded to stimulus, was recorded by the pixel, and the distance(mm) from the fovea was calculated by the Bennett formula. The maximum distance from the fovea at the given stimulus size was achieved as follows: 0.32+/-0.01mmat the 15 x15 arc of minute(0.333), 0.63+/-0.01mm at the 17 x17 arc of minute(0.294), 1.05+/-0.03 mmat 20 x 20 arc of minute(0.25), and 1.44+/-0.0 5 mmat the 23 x23 arc of minute(0.217). It was also revealed that the horizontal maximal distance from fovea at given stimulus size was statistically superior to the vertical maximal distance(p<0.05). In conclusion we were able to establish the normal range of parafoveal retinal acuity in healthy volunteers. It may serve as the baseline for subsequent study of retinal pathology and functional evaluation as well as its treatment.
Healthy Volunteers* ; Pathology ; Reference Values ; Retinaldehyde* ; Visual Acuity*