A 21-year-old Korean male was referred to our department in June, 1999 for the evaluation of extensive reddish patches and gray-bluish pigmentation since birth. Physical examination revealed two kinds of patches over the various parts of the body. Reddish patches suggesting nevus flammeus were located on the left side of face, chest, and both upper and lower extremities. Gray-bluish pigmented patches suggesting nevus of Ota were found on both the periorbital areas. A 0.5×0.6 cm erythematous papule was found on the right anterior chest. The results of its histopathologic examination were compatible with pyogenic granuloma. We made the diagnosis of phakomatosis pigmentovascularis associated with pyogenic granuloma as well as Sturge-Weber syndrome and Klippel-Trenanunay syndrome.
Diagnosis ; Granuloma, Pyogenic* ; Humans ; Klippel-Trenaunay-Weber Syndrome* ; Lower Extremity ; Male ; Neurocutaneous Syndromes* ; Nevus of Ota ; Parturition ; Physical Examination ; Pigmentation ; Port-Wine Stain ; Sturge-Weber Syndrome* ; Thorax ; Young Adult

No abstract available.
Animals ; Embryonic Development* ; Embryonic Structures* ; Female ; Mice* ; Pregnancy

No abstract available.
Eating ; Functional Food ; Hypertrichosis ; Minoxidil

No abstract available.
Hepatitis E virus* ; Hepatitis E* ; Hepatitis* ; Korea*

In humans, the major stimulus for cutaneous pigmentation is ultraviolet radiation. Little is known about the mechanism underlying this response, in part, because of the complexity of the interactions involving the whole epidermis. The present stucy was undertake to evaluate the effects of a single exposure of UVB on cultured normal melanocytes. Melanocytes were exposed to UVB from 5.1 mJ/cm to 203 mJ/cm. The results were as follows : 1. The main morphologic changes in UVB-exposed groups w re larger sized cells, more blunted dendrites, and shorter dendrites than in the control group. These cells increased sized according to the increased doses of VVB, but above 101.5 mJ/cm, the melanocytes shrunk and were destroyed. 2. From 20.3 mJ/cm of UVB, the proliferation of melanocyte was decreased, Especially, there was statistical!y significant difference above 50.8 mJ/cm (p<0.05, p<0.01). 3. The antiproliferativo effect increased with the passage of tirie after UVB exposure. So, cell count could not be done in 101.5 mJ/cm and 203 mJ/cm on the third day, and in 50.8 mJ/cm, 101.5 m J/cm and 203 mJ/cm on the seventh day. 4. Statistically the melanin content per well was significantl dicreased to 11-28% of each control group with dose above 50.8 mJ/cm (p<0.05, p<0.01). The melanin content per cell was increased to 107-128% of each control group when doses were below 20.3 mJ/cm and decreased to 49-79% of each control group when above 0.8 mJ/cm on the third day, but there was no statistically significant difference. In summary, when melarocytes were exposed to UVB, morphclogic changes progressed to cell differentiation. The results also suggested that a low or dose of UVB has an antiproliferative arid mild melanogenic effect, and a higher dose of UVB has a direct cytotoxic effect.
Cell Count ; Cell Differentiation ; Dendrites ; Epidermis ; Humans ; Melanins ; Melanocytes* ; Pigmentation

The author evaluated the optimal concentration of 3 compositions of TIC medium which has used as the melanacytes culture medium. The concentrations of placental extract and bovine pituitary extract, which have the ability to promote proliferation of melanocytes, were evaluated also. Modified TIC medium with above 5 components of evaluated concentration was very effective in melanocytes culture. The results were as follows : l. 12-0-tetradecanoyl-phorbol-13-acetate (TPA) showed effective melanocytes proliferating activity at the concentration of 30ngml (p(0.05) 2. Isobutylmet:hyl xanthine (IBMX) showed effective melanocytes proliferating activity at the concentration of 0.3mM (p(0.05) 3. Cholera toxin (CT) showed effective melanocytes proliferating activity at the concentration of )OnM (p(0.05) 4. Two percentages of placental extract in culture medium showed effective melanocytes proliferating activity. S. Two percentages of bovine pituitary extract in culture medium showed effective melanocytes proliferating activity. 6. Placental extract and isobutylmethyl xanthine proved to have high melanocytes proliferating activity. 7. Melanocytes proliferated rapidly on modified TIC medium (Proliferation doubling time . about 43 hours) 8. The peak time of melanocytes proliferation (7.2 X 10/cm) was observed on the seventh day of culture, From this data, this culture system can be recommended as a new melanocytes culture.
Cholera Toxin ; Humans* ; Melanocytes* ; Tics ; Xanthine

Squamous cell carcinoma(SCC) is a recognized late complication of chronic discoid lupus erythematosus(CDLE). There are many case reports of SCC in white patients with chronic DLE. However, it is uncommon in blacks and Asians. The etiology of squamous cell carcinoma is multifactorial. The predisposing factors for the development of SCC in these patients include actinic keratosis, Bowen's disease, burn scars, arsenic keratosis, and chronic discoid lupus erythematosus. Of these causes, SCC developing in the lesions of CDLE is very rare. We report a patient who has been evaluated for ten years with a diagnosis of discoid lupus erythematosus and has squamous cell carcinoma.
African Continental Ancestry Group ; Arsenic ; Asian Continental Ancestry Group ; Bowen's Disease ; Burns ; Carcinoma, Squamous Cell* ; Causality ; Cicatrix ; Diagnosis ; Humans ; Keratosis ; Keratosis, Actinic ; Lupus Erythematosus, Discoid*

BACKGROUND: The main function of melanocyte is known to protect the skin from hazardous sunlight. But, some investigators have claimed lately that melanocytes are also related to the immunologic role in the epidermis because these cells produce IL-1 activity and IL-1beta convertase activity, in vitro. OBJECTIVE: Our purposes were to investigate the effects of rIFN-gammaon the proliferation of melanocytes, melanin content, and the expression of HLA-DR antigen on melanocytes after a rIFN-gammaexposure. MEHTODS: The number of melanocytes, the melanin content, and the expression of HLA-DR antigen were evaluated on cultured human melanocytes according to a time sequence and various concentrations of rIFN-gamma. RESULTS: Antiproliferative activity on melanocytes was dependent on the exposure time and the concentration of rIFN-gamma. According to the exposure time and the concentration of rIFN-gamma, melanogenic acivity was inhibitd or stimulated. Normal melanocytes didn't express HLA-DR antigen, but when normal melanocytes were exposed to rIFN-gamma, the expression of HLA-DR antigen increased in a time-and concentration-dependent fashion. After the removal of rIFN - gammafrom the culture media, the expression of HLA-DR antigen on melanocytes also disappeared. CONCLUSION: In our study, melanocytes seem to be related to the immunologic role in the epidermis because these cells expressed HLA-DR antigen after rIFN-gammaexposure and we think that study could help to investigate between melanocytes and immunologic mechanisms in various inflammatory skin diseases.
Culture Media ; Epidermis ; HLA-DR Antigens* ; Humans* ; Interleukin-1 ; Melanins ; Melanocytes ; Research Personnel ; Skin ; Skin Diseases ; Sunlight

Benign symmetric lipomat,osis is a rare disease of undetermined cause characterized by symmetric deposits of adipose tissue o:n the neck, upper trunk and abdomen. We report three cases of kienign symmetric lipomatosis, one of which is associated with alcoholic liver disease and the patient complained of a diffieulties in neck motion, All of them are about sixty year old men with history of frequent alcohol consumption. In histopathologic findings, the reticular dermis was completcly replaced by mature fat cells and there was a slight increase in vascular and fibrous elements.
Abdomen ; Adipocytes ; Adipose Tissue ; Alcohol Drinking ; Dermis ; Humans ; Lipomatosis* ; Liver Diseases, Alcoholic ; Male ; Neck ; Rare Diseases

Clinically, folliculosebaceous cystic hamartoma (FSCH) lacks distinct features, but it has been reported as an asymptomatic, 1- to 3-cm, dome-shaped nodule on the face. Histopathologically, FSCH is characterized by adnexal and folliculosebaceous cystic proliferation with various mesenchymal changes. This case presented an unusually large, small-fist-sized mass in the right mandibular area that was accompanied by intermittent itching. Histopathologic findings demonstrated appropriate features of FSCH. We present an interesting case of giant FSCH in a 48-year-old female.
Female ; Hamartoma ; Humans ; Middle Aged ; Pruritus