No abstract available.

No abstract available.
Infusions, Intraosseous* ; Resuscitation* ; Shock, Hemorrhagic*

Bowens disease is generally regarded as a premalignant dermatosis. If untreated, 3% to 5% of patients may develop squamous cell carcinoma. However, sebaciou carcinoma arising from Bowens disease is very rare. We presented a case of quarnous cell carcinoma and sebaceous circ s disease in a 68 year-old male. He had multiple bowenoid skin lesi nsties. A bean-sized nodule as developed on the bowenoid lesion of the he had a large yellow crust.ed exudative tumor on the Rt. thigh. We took a biopsy specimen of these three discrete lesions. The nu lipid stain of frozen section revealed Bowens disease, squarnous concllnoma arising from Bowen on the trunk and extremi Rt. lower abdomen. Almost of routine histology and carcinoma, and sebaceous carcinoma, respectively.
Abdomen ; Aged ; Biopsy ; Bowen's Disease ; Carcinoma, Squamous Cell* ; Frozen Sections ; Humans ; Male ; Skin ; Skin Diseases ; Thigh

PURPOSE: Long-term administration of anticonvulsants for pediatric epileptic patients may cause changes of bone metabolism. This study was performed to evaluate the influence of antiepileptics on the bone metabolism and growth in pediatric patients who received antiepileptics. Methods for early detection of adverse bone metabolism were also evaluated. METHODS: Forty-eight epileptic patients were enrolled in this study. All subjects had taken antiepileptics for at least 6 months. Subjects were grouped according to the antiepileptics : Group I(n= 31) was treated with carbamazepine(CBZ), Group II(n=9) used valproic acids(VPA), Group III(n=8) received multi-drugs including CBZ, VPA. We determined serum osteocalcin and total alkaline phosphatase levels as markers of bone formation, and urinary deoxypyridinoline as marker of bone resorption. Blood and urine samples were collected before the initial medication and follow up at least 6 months later. Serum osteocalcin and urinary deoxypyridinoline were measured by an ELISA method, respectively. Data from subjects were analyzed statistically by SPSS(ver. 10). RESULTS: Concentrations of serum osteocalcin and urinary deoxypyridinoline were significantly increased in the carbamazepine treated group. The increment of deoxypyridinoline positively correlated with the duration of treatment, especially in children who received carbamazepine therapy for more than 12 months(P<0.05). The serum concentration of calcium, phosphorus were within normal ranges, but the levels of alkaline phosphatase were significantly increased in all group(P<0.05). CONCLUSION: Our data suggests that serum osteocalcin and alkaline phosphatase, urinary deoxypyridinoline could be useful markers for the early detection of harmful bone metabolism during antiepileptic treatment.
Alkaline Phosphatase ; Anticonvulsants* ; Bone Resorption ; Calcium ; Carbamazepine ; Child* ; Enzyme-Linked Immunosorbent Assay ; Follow-Up Studies ; Humans ; Metabolism ; Osteocalcin ; Osteogenesis ; Phosphorus ; Reference Values

OBJECTIVE: The purpose of this study was to evaluate the efficacy and effect of various cryopreservation method on the survival and the cytoskeletal stability of metaphase II mouse oocyte. METHODS: Mouse ovulated oocytes were collected and cryopreserved by a modified slow-freezing method with 1.5 M 1,2-propanediol (PrOH)+0.1 M sucrose or by vitrification using cryo loop and EM grid with 40% ethylene glycol+0.6 M sucrose. Four hours after thawing, intact oocytes were fixed and stained with fluorescein isothiocyanate (FITC)-conjugated monoclonal anti-beta-tubulin antibody to visualize spindle and propidium iodide (PI) to visualize chromosome. Spindle morphology was classified as follows: normal (barrel-shaped), slightly and absolute abnormal (multipolar or absent). RESULTS: Survival rate of the frozen-thawed oocytes in vitrification group was significantly higher than that of slow-freezing group (62.7% vs. 24.4%, p<0.01). Vitrification with cryo loop showed significantly higher survival rate than that with EM grid (67.7% vs. 53.5%, p<0.05). On the other hand, proportion of normal spindle and chromosome configurations of the frozen-thawed oocytes between two vitrification group was not significantly different. CONCLUSION: For mouse ovulated oocytes, vitrification with cryo loop may be a preferable procedure compared to slow-freezing method. Further study should be needed to investigate developmental competency of frozen-thawed mouse oocytes.
Animals ; Cryopreservation* ; Fluorescein ; Hand ; Metaphase ; Mice* ; Oocytes* ; Propidium ; Propylene Glycol ; Sucrose ; Survival Rate ; Vitrification

Miller-Dieter syndrome consists of severe type I lissencephaly, abnormal facial appearance, and sometimes other birth defects. Lissencephaly is a brain malformation manifested by a smooth cerebral surface, thickened cortical mantle, and microscopic evidence of incomplete neuronal migration. It comprises the agyria-pachygyria spectrum of malformation, thus excluding polymicrogyria and other cortical dysplasia. Type I lissencephaly results from abnormal migration between about 10 and 14 weeks gestaion. The brain is often small, and the ventricle is enlarged posteriorly The corpus callosum may be small or absent. The structural pattern of the cerebral hemispheres and ventricles is distintly immature, reminiscent of fetal brain. The superficial cellular layer resembles an immature cortex, with some separation into zones similar to layers III, V, and VI of normal cortex, although the cell population is decreased. In 1963 Miller described a malformation syndrome in a brother and sister with postnatal growth deficiency, craniofacial defects, and serious abnormalities of neurologic function. Autopsy at 3 and 4month of age, respectively, revealed lissencephaly. Subsequently, Dieker reported four additional patients with this disorder and referred to it as the 'lissencephaly syndrome'. We have experienced a case with this syndrome. Then we report this rare case with brief review of literature.
Autopsy ; Brain ; Cerebrum ; Congenital Abnormalities ; Corpus Callosum ; Humans ; Lissencephaly ; Malformations of Cortical Development ; Neurons ; Siblings

Cervical disc arthroplasty (CDA) has become more widespread and diverges from the conventional technique used in anterior cervical fusion for cervical degenerative disc disease. As arthroplasty has become a popular treatment option, few complications have been reported in the literature. These include subsidence, expulsion, posterior avulsion fractures, heterotopic ossification, and osteolysis. One of the critical complications is osteolysis, but current studies on this subject are limited in terms of not elucidating the incidence, etiology, and consequences. The authors present two cases, who presented with clinical signs of gradually worsening myelopathy induced by posterior vertebral body osteolysis, 2 years after CDA. Subsequently, the patient underwent posterior decompression and fusion without prosthesis removal. Postoperatively, the clinical symptoms gradually resolved, with no severe deficits. The present rare cases highlight the osteolysis that occurs after CDA, which can cause cervical myelopathy, and suggest spine surgeons to be alert to this fatal complication.

PURPOSE: Acute administration of non-selective serotonin (5-HT) reuptake inhibitor such as imipramine and selective 5-HT reuptake inhibitor, like fluoxetine leads, to an increase of extracellular 5-HT concentration in the brain. We sought to determine the average time spent asleep, the frequency of REM sleep, and the percent of REM and NREM sleep in the total sleep time in saline (sham-treated control) (n=6), imipramine (n=8), or fluoxetine (n=6)-treated animals. METHODS: Right and left cortical and hippocampal electrodes were placed in 10-15 day old Sprague-Dawley rats. The following day 2 hour video EEG recordings were done to monitor sleep induced by intraperitoneal injection of saline, imipramine (10mg/kg), or fluoxetine (10mg/kg) after getting a baseline EEG during 30 minutes. And data were analyzed using t-test. RESULTS: 1) Following intraperitoneal injection of saline, imipramine, or fluoxetine, there were no epileptiform features or changes in the EEG except for a difference in sleep cycling. 2) The percent of average time spent asleep was significantly greater for control (87.8%) and fluoxetine-treated animals (92%) compared to imipramine-treated animals (66.1 %) (p<0.005). 3) The average frequency of REM sleep was 11.2 in control, 0.1 in imipramine-treated animals, and 8.7 in fluoxetine-treated animals, respectively during the 2 hours. And the frequency of REM sleep was significantly less for imipramine-treated animals compared to control (p<0.002). 4) Control animals (41.2%) spent significantly less time in NREM sleep compared to imipramine (98.8%)- and fluoxetine (93%)-treated animals (p<0.0001) and significantly more time (58.8%) in REM sleep compared to both imipramine (1.2%)- and fluoxetine (7%) treated animals (p<0.0001). CONCLUSION: We confirmed that postnatal 10-15 day old rats spent more time in REM sleep than NREM sleep, and acute administration of imipramine or fluoxetine increased NREM sleep by decreasing the frequency and the duration of REM sleep by 5-HT reuptake inhibition in the brain.
Animals ; Brain ; Electrodes ; Electroencephalography ; Fluoxetine* ; Imipramine* ; Injections, Intraperitoneal ; Rats* ; Rats, Sprague-Dawley ; Serotonin ; Sleep, REM

Malignant histiocytosis is a rare, usually fatal malignant neoplasm of reticuloendothelial systems. The disease is associated with fever, malaise, weight loss, hepatosplenomegaly, lymphadenopathy, pancytopenia, jaundice, and purpura. A 44-year-old female patient is described who had multiple, purple crusted nodules and plaques in the skin. In the laboratory study, pancytopenia was noted on the peripheral blood. In addition many atypical histiocytes were seen on the bone marrow aspiration. A lesional biopsy showed nodular infiltrations of atypical histiocytes in the dermis and some erythrophagocytosis was seen. Immunohistochemically, the histiocytes were weakly stained for lysozyme and α-l-antichymotrypsin, but were unstained for S-100 protein, cytokeratin, CEA(carcinoembryonic antigen), pan T/B marker CD30(ki-1), UCHL-1 LCA(leukocyte common antigen), and α-l-antitrypsin.
Adult ; Biopsy ; Bone Marrow ; Dermis ; Female ; Fever ; Histiocytes ; Histiocytic Sarcoma* ; Humans ; Jaundice ; Keratins ; Lymphatic Diseases ; Mononuclear Phagocyte System ; Muramidase ; Pancytopenia ; Purpura ; S100 Proteins ; Skin ; Weight Loss

PURPOSE: This study aims to understand the worries that patients and families with a history of colorectal cancer experience. METHODS: From January 2011 through January 2013, in-depth interviews and observations were conducted on 3 colorectal patients and 8 family members with a family history of colorectal cancer. The data were analyzed using a micro-ethnographic research method. RESULTS: After colorectal cancer patients and families realized and concerned with their increased chance of getting cancer, Eventually this worry became a motivator causing them to adhere to cancer preventive behavior. Three types of cancer worries were identified: confrontation, uneasiness, and warning. Depending on the type of cancer risk, psychological responses and cancer preventive behavior were found to be varied according on the three types of cancer worries. Patients and families tried to change dietary habits and regulary exercise. To help ease their cancer worries, they started to search for information on colon cancers and attempted to follow recommendations. However, they found that neither detailed guidelines nor systematic management from health professionals existed for families with colon cancer patients. CONCLUSION: A cancer prevention nursing program needs to be developed based on the level of cancer risk and perceived cancer worries of clients.
Colonic Neoplasms ; Colorectal Neoplasms* ; Early Detection of Cancer ; Food Habits ; Health Occupations ; Humans ; Methods ; Nursing