1.Antiproliferative effect of difluoromethylornithine on human gastric and colorectal cancer cell lines in serum containing media.
Journal of the Korean Cancer Association 1992;24(3):359-364
No abstract available.
Cell Line*
;
Colorectal Neoplasms*
;
Eflornithine*
;
Humans*
2.Study of Skin Disorders in Alcoholic Patients.
Korean Journal of Dermatology 1990;28(5):575-581
No abstract available.
Alcoholics*
;
Humans
;
Skin*
3.Storage of the split-thickness skin piece using proper antibiotics.
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1998;25(6):997-1002
Todays, remnant split-thickness skin graft is stored for graft failure or for delayed grafting. Refrigerated skin is usually stored for 3 weeks, after which, cellular respiration ceaces. Even though the refrigerated skin can be used before 3 weeks after harvest, the success rate of the skin graft is usually lower than in case of fresh skin. One of the most reliable explanations is multiplication of microorganisms on the stored skin, that is, the more microorganisms on the refrigerated skin, the less the success rate of grafts. For this reasons, some kind of antibiotics have been used for storage of the split-thickness skin piece. But there is no report about the effect of antibiotics on stored skin. We want to know the effect of the antibiotics on stored skin. For this purpose, we did three experiments for qualititative bacteriology of refrigerated skin. Experiment 1 was qualititative identification of microorganisms colonizing split-thickness skin after 2 weeks storage in low temperature, and sensitivity tests for identified microorganisms. On the basis of experiment 1, the proper antibiotics were selected and samples of split-thickness skin were stored using this antibiotics. At 2 weeks after storage in low temperature, samples of split-thickness skin were cultured for identification of bacterial growth. This is experiment 2. Experiment 3 is histologic examination of the split-thickness skin involved in experiment 1 and 2.In the experiment 1, we found five kinds of microorganisms in 9 out of 30 split-thickness skin samples. The most common microorganism was coagulase negative Staphylococcus which was found in 4 samples. Through the antibiotics sensitivity test, teicoplanin was selected as the most proper antibiotics. In experiment 2, we could not find any microorganisms in 30 split-thickness skin samples. In experiment 3, there were no histologic differences in the split-thickness skin samples whether antibiotics were used or not. Through these results, we have confirms that split-thickness skin pieces are more safely stored using proper antibiotics.
Anti-Bacterial Agents*
;
Bacteriology
;
Cell Respiration
;
Coagulase
;
Colon
;
Skin*
;
Staphylococcus
;
Teicoplanin
;
Transplants
4.Clinical Application of Polymerase Chain Reaction for Diagnosis of Pulmonary Tuberculosis in Pneumoconiotic Patient.
Jae Hee PARK ; Chul Jae LIM ; Kyung Hye LEE
Korean Journal of Occupational and Environmental Medicine 1998;10(1):20-28
Recent development in thepolymerase chain reaction (PCR) has brought an extraordinary opportunity for the rapid detection of M. tuberculosis in clinical specimens for the diagnosis of tuberculosis. Pneumoconiosis is a sort of pulmonary fibrosis consequent to inhalation of the respirable dust. The association between pulmonary tuberculosis and pneumoconiosis is well recognized. There is a 10-fold increase in the tuberculosis risk among the workers who have pneumoconiosis demonstrated by chest roentgenogram. The physicians managing the patients with pneumoconiosis have to maintain a high index of suspicion for the development of mycobacterial infection, since the diagnosis of tuberculosis is often difficult. Mycobacterium tuberculosis is a very slow growing organism and acid-fast bacillus (AFB) staining frequently shows false negative results, and therefore PCR would be a very rapid, easy and sensitive diagnostic method for the diagnosis of Mycobacterium tuberculosis in pneumoconiotic patients. To compare the PCR method with the conventional methods in diagnosing Mycobacterium tuberculosis in sputum, we used the sputa of 115 pneumoconiosis patients in Munkyeong Cheil Hospital. Of 32 pulmonary tuberculosis in the pneumoconiosis patients, 29 were PCR positive and were higher than 28, 20 positive by culture and AFB stain. Overall sensitivity, specificity, and which were 90.6, 91.5 % respectively for the PCR assay, 87.5, 100 % for the culture method ; 62.5, 98.7 % for the AFB stain. The PCR assay is a rapid, efficient, sensitive method which can detect M. tuberculosis directly in pneumoconiosis patients, and further study should be followed for the development of the easier method.
Bacillus
;
Diagnosis*
;
Dust
;
Humans
;
Inhalation
;
Mycobacterium tuberculosis
;
Pneumoconiosis
;
Polymerase Chain Reaction*
;
Pulmonary Fibrosis
;
Sensitivity and Specificity
;
Sputum
;
Thorax
;
Tuberculosis
;
Tuberculosis, Pulmonary*
5.Variation of class I antigen(HLA-ABC), class II antigen(HLA-DR) and T6 positive cells according to the culture of epidermal cells.
Ho Chul PARK ; Won Joong KIM ; Jae Kyung PARK
Journal of the Korean Surgical Society 1993;45(4):453-464
No abstract available.
6.A study on the long-term preservation of artificial skin.
Ho Chul PARK ; Hyung Soo LEE ; Jae Kyung PARK
Journal of the Korean Surgical Society 1993;45(5):618-632
No abstract available.
Skin, Artificial*
7.Traumatic Lipoma.
Kwang Young PARK ; Jae Kyung PARK ; Sang Won KIM
Annals of Dermatology 1991;3(1):77-79
The occurrence of a lipoma-like condition associated with trauma would tend to be regarded as a subset of or a separate entity from ordinary lipoma. We report herein a case of traumatic Lpoma occuring on the dorsum of the left hand. The patient was a 46-year-old male, who had struck his left hand against a window about two months previously and noted casually an asymptomatic, firm, dermal lesion at the site of injury. On gross examination, the enucleated nodule was a smooth-surfaced, yellow fatty mass measuring 0.3 × 0.5 × 0.3cm which looked like a kernel of corn. Microscopic examination revealed encapsulated mature fat consistent with lipoma.
Hand
;
Humans
;
Lipoma*
;
Male
;
Middle Aged
;
Zea mays
8.Immune complex-induced increases in collagen production by cultured mesangial cells modulated by dexamthasone and heparin.
Chun Gyoo IHM ; Jae Kyung PARK ; Jae Hyung AHN ; Tae Won LEE ; Myung Jae KIM
Korean Journal of Nephrology 1992;11(3):207-213
No abstract available.
Collagen*
;
Heparin*
;
Mesangial Cells*
9.Effects of glucose, insulin and somatostatin on collagen production by glomerular mesangial cell.
Chun Gyoo IHM ; Jae Kyung PARK ; Jae Hyung AHN ; Tae Won LEE ; Myung Jae KIM
Korean Journal of Nephrology 1992;11(3):200-206
No abstract available.
Collagen*
;
Glucose*
;
Insulin*
;
Mesangial Cells*
;
Somatostatin*
10.A Case of Transepidermal Elimination in Gouty Tophus.
Jae Kyung PARK ; Chan KANG ; Sang Won KIM ; Jae Bok PARK
Korean Journal of Dermatology 1990;28(1):85-89
We herein report a case of gout, who was a 45-year-old man and had had multiple various-sized papules or tophi on the helices of ears, hands, elbows, knees, ankles and feet for 10 years. Histopathologic findings of the large tophus of right knee revealed the typical pattern of gout with needle-shaped urate crystal. The phenomenon of iransepidermal elimination was observed in serial sections of histologic speeirnerls, The nsrrow transepidermal channel, lined by pseudoepitheliomstous lips bilaterally, was Eilled with the cellular debris and urate crystal fragments and cornmunicated direotly with the upper dermis. Presumably, transepidermal elirnination in gouty tophus may be the phenomenon whieh could be found rnore commonly than the scarcity of literature.
Ankle
;
Dermis
;
Ear
;
Elbow
;
Foot
;
Gout
;
Hand
;
Humans
;
Knee
;
Lip
;
Middle Aged
;
Uric Acid